The interaction between heart and brain has drawn more and more attention in recent years. The concept of heart-brain inte-gration has been gradually established, which means the interaction in physiology and pathology between the two organs. On the basis of nerve-fluid system, sympathetic nervous system and renin-angiotensin-aldosterone system (RAAs) are involved in the modulation of heart and brain. As review of the ancient literature of China, heart and brain were considered as the organs of cognition and behavior, which are the functions of only brain from modern science. Several recent researches supported the opinions of ancient Chinese scholars in some ways.

Objective To study the expression of malignant melanoma(MM) related genes by cDNA microarray technique. Methods mRNA, extracted from tissues of patients and normal controls, was reversely transcripted into cDNA and marked with 33P. The cDNA probes were hybridized to cDNA microarrays, which contained 2000 human genes each array. The down-regulation of two co-differentiated expressed genes was confirmed by quantitative real-time RT-PCR. Results Different expression between MM and normal controls was found in 4.7%-6.15% of genes by more than 2 times,0.75%-1.4% by more than 5 times, and 0.45-0.5% by more than 10 times. These genes were pro-oncogenes, tumor suppressor genes, genes related to apoptosis, cell cycle related genes, and so on. Three genes were down-regulated in all of the patients. Two of those genes, histidine triad nucleotide-binding protein (HINT) and RBP1-like protein (BCAA), were down-regulated, as identification by quantitative real-time RT-PCR. Conclusions cDNA microarray can be used effectively to reveal melanoma gene expression profiling for the propose of carcinogenesis study. HINT and BCAA are the first reported genes down-regulated in MM. However, further studies are needed for their expressive specificity and mechanism in MM.

Hepatocyte growth factor receptor c-Met and epithelial-mesenchymal transition (EMT) are closely related to the hepatocellular cancer.Recent researches have found that the mechanism of c-Met regulating EMT may play an important role in the carcinogenesis of hepatocellular cancer,which becomes a new research hot topic,Now we overviewed the progress on the mechanism of c-Met regulating EMT in hepatocellular cancer.

Bronchopulmonary dysplasia(BPD)is a serious complication of respiratory system in preterm infants.The etiology and pathogenesis are complex and have not been clarified yet.In recent years, studies have shown that there is a certain relationship between respiratory microecology and BPD.Before premature infants develop BPD, their respiratory tract microecology has changed, including abnormal microbial diversity and evolution pattern.Therefore, research on the colonization and evolution of neonatal respiratory tract microecology and its relationship with BPD is expected to provide new ideas for its prevention and treatment.

Objective To explore the clinical characteristics and management of urinary tract injuries incidental in gynecologic surgery. Methods Urinary tract injuries in gynecologic surgery during the past 10 years were reviewed retrospectively.The clinical features of initial operations including the types of disease,operative procedures and the methods of diagnosis and treatment was studied. Results 21 urological injuries were incurred during the performance of 6075 gynecologic surgical procedures,an incidence of 0.35% including of 10 ureter injuries and 11 bladder injuries with incidence of 0.16% and 0.18% respectively. The time of diagnosis from 0 to 23 days postoperatively.As for operation way,Laparoscopic surgery 10 cases(48%),radical surgery for cancer 7 cases(33%),other surgery 4 cases.injury of urinary tract was found intraoperatively in 15 patients(67%) and postoperatively in 7 patients(33%).Urinary fistulae occurred in 6 patients(29%).Urinary tract injuries were mainly diagnosed methylene blue infusion,via excretory urogram(IVP),CTU.An appropriate repair during operation,putting the doubleJ-catheter and catheterization was useful. Conclusion The rate of urinary tract injury is increased as more patients received laparoscopic surgery.Most of urinary tract injuries in gynecologic surgery had optimal results when they were diagnosed early and managed correctly.

Objective To explore the effects of Acer truncatum oil on oxidative stress and inflammatory reaction in rats after exhaustive exercise to provide a scientific basis for the prevention of sports injury by Acer truncatum oil.Methods Thirty healthy male SD rats were randomly divided into control group (A,normal diet + saline every day),exhaustive group (B,normal diet + saline daily) and exhaustive with Acer truncatum oil group (C,0.21 mL/kg daily Acer truncatum oil gavage),6 weeks later,the rats performed once exhaustive swimming exercise,then the creatine kinase (CK),lactate dehydrogenase (LDH),cytokine interleukin (IL-1β,IL-10) and tumor necrosis factor alpha (TNF-α) in the serum;malondialdehyde (MDA) homogenate reduction glutathione (GSH),superoxide dismutase (SOD),catalase (CAT) and glutathione peroxidase (GSH-Px) in muscle were measured.Results After exhaustive exercise,when compared with the group A,the serum levels of CK,LDH,IL-1,TNF-α and IL-10 were significantly higher than in the group B(P＜0.05,P＜0.01,P＜0.05,P＜0.01,P＜0.05),IL-10/ TNF-α ratio had no statistically significant difference(P＞0.05),the level of MDA in gastrocnemius muscle increased significantly (P＜0.05),GSH level and GSH-PX activity decreased significantly (P＜0.05,P＜0.01),SOD and CAT activity were not statistically significant (P＞0.05).when compared with the group B,serum CK,LDH level and IL-1 β,TNF-α were significantly decreased (P＜0.05,P＜0.01,P＜0.01,P＜0.05),IL-10 had no statistical significance (P＞0.05),a significant increase in IL-10/TNF-α(P＜0.05),the level of MDA of gastrocnemius muscle decreased significantly (P＜0.05),the level of GSH and SOD,CAT and GSH-PX were significantly increased (P＜0,01,P＜0.01,P＜0.05,P＜0.01).Conclusion Acer truncatum oil can significantly reduce oxidative stress and inflammatory reaction caused by the exhaustive exercise,which has a protective effect on skeletal muscle.

This paper briefly introduced the characteristics, origin and development of virtual reality, and the value for rehabilitation; reviewed the application of virtual reality in the rehabilitation of balance function after stroke. Some assessment commonly used for balance function were summarized.

Haplotypes ; Humans ; Neoplasms ; genetics ; Polymorphism, Single Nucleotide ; genetics

Colorectal cancer is a common malignant tumor of digestive tract, and the risk of inflammatory bowel disease developing into colorectal cancer is significantly increased. Immune signaling pathways NF-κB, IL-6/STAT3, COX-2/PGE2, IL-23/Th17 and TLRs have been confirmed to promote the transformation from colitis to colorectal cancer. NOD2 and intestinal microbes also participate in the regulation of inflammation mediated carcinogenesis. Chronic inflammation is a potential risk for colorectal cancer, and anti-inflammatory drugs may play a chemical preventive role. In this review, we summarize the signaling pathways involved in inflammation-associated colon carcinogenesis and evaluate the chemoprophylaxis of colon cancer.

Objective:To obtain apoptin gene and to induce tumor cell (HeLa) apoptosis. Methods:Apoptin gene was amplified by PCR from CAV TK5803 genomic DNA,and was then cloned into pCDNA3.1/His/Topo vector. After confirming by DNA sequencing, apoptin gene was subcloned into pCIneo vector.Recombinant plasmid pCDNA3.1/His/Topo-apoptin and pCIneo-apoptin were transformed into HeLa cells by Lipofectamine reagent. Three days after transfection,the HeLa cell was stained by Honchest 33258 and apoptosis was analysed by fluorescence microscope. Results and Conclusions: The full-length apoptin gene was cloned by PCR and inserted into pCDNA3.1/His/Topo vector. Sequence analysis demonstrated that it was same as published apoptin gene sequence. Three days after transfection, HeLa cell turned into apoptosis.