Objective] To explore the relationship between the pathogenesis theory of Qi and blood and stroke. [Method] The system research of ancient literature on the pathogenesis of stroke, analysis of stroke from the relationship between Qi and blood and stroke, focus on exploring the basic role of Qi and blood in the onset of stroke. [Results] Before the Tang and Song Dynasties doctors hadargument in the imaginary evilstroke pathogenesis;Jin era, that the internal gas is stroke, or qi deficiency Erzhi phlegm blockage;Ming and Qing Dynasties, many from the deficiency of Qi and Blood Stasis Theory, gas, blood throughout the stroke always. Supplementing qi and activating blood circulation method can improve the clinical symptoms of patients with stroke, stroke with Qi and blood as the pathogenesis treatment, clinical therapeutic effect. [Conclusion] Stroke syndrome differentiation from the blood, more can promote blood regulating. The stroke engine has extremely important theoretical and clinical significance from the blood, can provide a theoretical basis for the treatment of stroke in different stages, and guide the clinic, it is worth further study.

0.05),but all the outcome measures at 1 month in the treatment group were significantly improved as compared with control group(P0.05).The mean light sensitivity in the treatment group was better than in control group(P

Objective To explore the changes of blood 8-iso-Prostaglandin F_(2a)(8-iso-PGF_(2a))content in different time after building-model and study its significance,and study the intervention effect of N-acetylcysteine(NAC) on it.Methods Seven-day-old Sprague-Dawley (SD) rats were randomly divided into 3 groups:intervention,operation and false operation groups.rats in intervention group were administered with NAC 0.1 mg/(g?d),Rats in operation group were given an equal volume of normal saline,and rats in false group operation had no administration.The blood was obtained to determine serum of 8-iso-PGF_(2a) content in 30 minutes,on the first,third,seventh and twenty first day after the building-model,respectively.EIA assay method was used to determine serum 8-(iso-PGF)_(2a) content.Results Serum 8-iso-PGF_(2a) concentration in rats from operation group,intervention and false operation groups were(168.7?(24.2)),(120.9?23.4) and (50.0?8.8) ng/L within 30 minutes after the building-model, respectively.There were a remarkable difference between serum 8-iso-PGF_(2a) concentration in three groups(P0.05).Conclusions The concentration of serum 8-iso-PGF_(2a) of rats rises remarkably early after building-model, and the peak concentration appears at 30 minutes after the building-model. NAC can effectively decrease serum 8-iso-PGF_(2a) content, which suggest that there is large value of clinical administration.

Adult ; Aged ; China ; epidemiology ; Female ; Gold ; Humans ; Male ; Middle Aged ; Mining ; Silicosis ; mortality ; Young Adult

Objective To explore the expression of beta 2 adrenergic receptors(?2-AR) in bone marrow cells and its significance and provide a scientific basis for the theory of hematopoietic regulation by nerve factors.Methods After bone marrow mononuclear cells were isolated from normal mouse bone marrow by the Ficoll density gradient centrifugation,the expressions of ?2-AR were studied by immunohistochemistry in bone marrow mononuclear cells,cultured bone marrow stromal cells,GM-CFUs,paraffin sections and smears of bone marrow.Mouse models of immune-mediated aplastic anemia(AA) were made by lymphocyte infusion.Mice were divided into 3 groups including animal model group,?2-AR antagonist (ICI 118551) group,and control group,and the expression of ?2-AR in bone marrow mononuclear cells at the 8 th day was detected by Western blotting.Results The positive expression of ?2-AR in cytoplasm or membrane of normal mouse bone marrow cells showed different brown by immunohistochemical staining.There were intensive positive expressions of ?2-AR in mononuclear cells(48%) and bone marrow stromal cells (68%),while progenitor cells only showed middle-positive(55%)or weak positive(45%),and other hematopoietic accessory cells were positive in varying degrees.The relative values of ?2-AR of bone marrow mononuclear cells in 3 groups were 1.03?0.31,1.72?0.29 and 1.41?0.35,respectively;the expression of ?2-AR in animal model group was more lower than that in control group(P

Objective To investigate the effect of chronic stress on the mucin secretion and muc2 protein content in goblet cells of rat colonic mucosa. Methods 24 healthy Wistar rats were randomly divided into 2 groups. Control group moved freely, experimental group was forced to undergo 2-hour-long immobilization stress per day for 14 days. The number of goblet cells containing mucus was determined by AB/PAS histochemical staining. The expression of muc2 protein was detected by immuohistochemical staining. Results Chronic stress decreased the number of goblet cells containing mucus, and down-regulated the expression of muc2 protein in goblet cells in rat colonic mucosa. Conclusion Chronic stress damaged the colonic epithelial barrier and functions of colonic goblet cells.

The distribution of hepatitis B virus genotype in Hubei province and its clinical significance were investigated. HBV genotypes of 276 patients were detected by PCR-microplate sandwich hybridization-ELISA technique. The level of HBV DNA was detected by using PCR-fluorescence quantification test. Among 276 patients, there were 78 cases of HBV asymptomatic carriers, 110 cases of chronic hepatitis B (CHB), 62 cases of severe hepatitis (SH) or liver cirrhosis (LC) and 26 cases of hepatocellular carcinoma (HCC). The genotypes of HBV included C, B, mixtures (B+C, B+D, C+D) and D, accounting for 55.8%, 25.4%, 16.7% and 2.1% respectively. The average level of HBV DNA in genotypes C, B, mixtures and D was 1.20x10(6), 7.81x10(4), 3.26x10(5) and 5.01x10(4) copies/mL respectively. The ratio of SH, LC and HCC in genotype B, C and mixtures was 20%, 30% and 48% respectively. Statistical analysis revealed the percentage of genotype mixtures infection was significantly higher than that of genotype B infection. There was no significant difference in the percentage between genotype B and genotype C or between genotype C and mixtures. The distribution of genotype B, C and mixtures in SH, LC and HCC was significantly different. The frequency of HCC was zero in patients with co-infection. Genotype D was only related with SH and LC. The increased ALT could be converted to categorical grades of severity. From mild, moderate to severity, the prevalence of genotype C showed an opposite trend, although no statistically significant difference was observed. The HBeAg positive rate was higher in patients with genotype C infection than in those with genotype B, especially in the patients whose ages were from 31 to 40 years old. Compared with genotype B, genotype C showed a higher HBeAg positive rate in patients with SH and LC. The percentage of SH, LC and HCC was higher in patients with genotype C and mixtures infection. On the contrary, the percentage of genotype B was lower. The HBeAg positive rate in patients with genotype C infection was higher than those with genotype B infection. Genotype C and mixtures may be associated with development of severe liver disease.

ObjectiveTo investigate the preventive effect of drugs imitating the ischemic preconditio ning to the donor lung.MethodsAdenosine was used to imitate the early protection of ischemic preconditioning, SOD to imitate the delayed protection and both of them to imitate the integral p rocess of ischemic preconditioning. At the same time, a control group was set up . The rabbits' lungs were flushed, the heart-lung blocs harvested, and adenosin e, SOD, both or none of them wadded respectively. After preservation for 6 h, th e lungs were reperfused for 1 h with the isolated rabbit lung reperfusion model. The PaO 2, airway pressure, pulmonary compliance, wet/dry ratio and content of NOS were measured. The microscopic and electron microscopic examinations were c arried out.ResultsPaO 2 values were the highest in the control group, followed by the SOD group, the adenosine group, the combined use group. The differences among the groups we re significant. The wet/dry ratio was the highest in the control group, followed by the adenosine group, the SOD group, the combined use group with the differen ces among the groups being significant. The airway pressure and NOS content in t he imitating ischemic preconditioning groups was lower than that of the control group. The pulmonary compliance of the imitating ischemic preconditioning groups was higher than that of the control group. However, the differences were not si gnificant. The microscopic and electron microscopic examination revealed that th e pathological changes in the imitating ischemic preconditioning groups was obvi ously lightened as compared with those in the control group.ConclusionsAdministration of the drugs imitating ischemic preconditioning could improve pul monary oxygenation function and ameliorate pulmonary edema. Although the drugs s howed less effect in ameliorating airway pressure, pulmonary compliance and NOS activity, the imitating ischemic preconditioning could protect the donor lung.

Objective To investigate the potential roles of pattern recognition receptor in the pathogenesis of asthma,the concentrations of serum nucleotide -binding oligomerization domain like receptors 1 (NOD1 )and Toll like receptor 1(TLR1)and Dectin and sCD14 protein were determined.Methods Blood samples were obtained from 45 cases of severe asthma(asthma group)during acute attack and convalescent period,the concentrations of serum NOD1,TLR1,Dectin and sCD14 protein were assessed using enzyme linked immunosorbent assay(ELISA),and compared with 30 healthy children(control group).Results The concentrations of NOD1,TLR1,Dectin and sCD14 protein were significantly higher in acute attack period of the asthma group[(65.53 ±19.95)ng/mL,(10.46 ±3.35)ng/mL, (80.38 ±19.51)ng/mL,(4.51 ±1.29)ng/mL]than those in the control group[(25.57 ±9.64)ng/mL,(5.54 ± 1.49)ng/mL,(47.37 ±15.16)ng/mL,(2.21 ±0.68)ng/mL](t =11.57,8.63,7.82,9.95,all P <0.01).Moreo-ver,the concentrations of serum NOD1,Dectin and sCD14 protein were dramatically decreased in convalescent period of the asthma group[(39.67 ±11.25)ng/mL,(60.04 ±16.76)ng/mL,(3.38 ±0.97)ng/mL]compared with acute asthma attack period(t =8.19,6.32,5.42,all P <0.01),while the concentration of serum TLR1 was not different between this two period (9.51 ±3.19)ng/mL(t =1.27,P >0.05).Furthermore,level of NOD1 was positively corre-lated with sCD14(r =0.49,P <0.01).Conclusion Levels of serum NOD1,TLR1,Dectin and sCD14 protein were increased in acute severe asthma,whereas,they were decreased in convalescent period.The elevation of them indicated that pattern recognition reception has synergistic function in the pathogenesis of asthma attack.

Objective To investigate the effect and safety of a low-dosed urokinase combined with fosinopri plus leflunomide and prednisone on treatment of moderate and severe IgA nephropathy (IgAN).Methods Fifty-seven patients with IgAN confirmed by biopsy combining with clinical presentations were enrolled,with their ages ranged from 15 to 55,proteinuria excretion ≥ 1.0 g/24 h,serum creatinine (SCr) ≤ 265.20 μmol/L,grade Ⅲ or above in Hass histologic grading systems for renal biopsy.The patients were randomly divided into trial group( n =28 ) and control group( n =29 ) and received treatment for six months.The patients in trial group were treated with a low-dosed urokinase combined with fosinopri plus leflunomide and prednisone,while those in control group were treated with prednisone and fosinopril.Results ( 1 ) After 6 months of treatment,the rates of complete remission (CR) and partial remission (PR) were 52.0％ and 40.0％ respectively in trial group,and 20.8％ and 54.2％ respectively in control group.The total remission rate(TR)and CR in trial group were significantly higher than those in control group ( TR x2 =0.47,P ＜ 0.05 ; CR x2 =5.11,P ＜ 0.05 ).( 2 ) The SCr level was decreased in trial group ( [ 115.83 ± 16.78 ] μmol/L v.s.(93.29 ±12.41 ) μmol/L,P ＜ 0.05 ),while increased in control group ( [ 112.79 ± 12.79 ] μmol/L v.s.( 136.99 ±25.97 )μmol/L,P ＜ 0.05 ).The two groups had significant difference on SCr clearance rate after treatment( P ＜0.05).There was no significant difference between the two groups on the endogenous creatinine clearance rate (Ccr) ( P =0.52 ),and no significant difference within trial group ( [ 79.34 ± 6.09 ] ml/min v.s.[ 85.12 ±12.26] ml/min,P =0.05)and within control group( [80.18 ±6.51 ] mi/min v.s.[84.22 ±8.39] ml/min,P =0.67 ).The levels of 24-hour proteinuria excretion after 6 months were decreased in both groups ( trial group:[1.93 ±0.55]g/24 hv.s.[0.78 ±0.42]g/24 h,P＜0.05;control group:[1.85 ±0.51]g/24 h v.s.[1.30±0.35 ] g/24 h,P ＜ 0.05 ),and the treatment effect was better in trial group ( P =0.04 ).Conclusion Treatment of moderate and severe IgA nephropathy with low-dosed urokinase combined with fosinopri plus leflunomide and prednisone is effective and safe by improving renal function and decreasing proteinuria.