[ ABSTRACT] Prenyl diphosphate synthase subunit 2 ( PDSS2), which encodes the second subunit of prenyl diphosphate synthase, an essential enzyme involved in the biosynthesis of coenzyme Q10 (CoQ10), is almost expressed in all tissues and organs at different developmental stages of human beings.The abnormal expression of PDSS2 may result in many diseases through impacting the biosynthesis of CoQ10.Recent studies show that PDSS2 gene has decreased in mela-noma, gastric cancer, lung cancer and hepatocellular carcinoma, and the degree of reduced level is closely related to the clinical features, which enlighten us that PDSS2 maybe a tumor suppressor gene involves in the development and progres-sion of carcinoma.This review aims to introduce the recent research progress about PDSS2 gene on carcinoma, discuss its roles and value on cancer research.

Asthenospermia accounts for about 30% of the causes of male infertility. Currently, most drugs for asthenospermia lack specificity and desirable therapeutic efficiency. An insight into the pathogenesis of asthenospermia is important for the development of specific therapies for this disease. The protein Na+/K(+)- ATPase α4 isoform (NKA4) presents in both mature testis tissue and the sperm tail, the absence or reduced activity of which may significantly decrease sperm motility. Ouabain is a natural inhibitor of NKA4, suppressing its activity by specifically binding the ouabain site in it. The hypothalamus and adrenal cortex excrete an ouabain-like steroid hormone called endogenous ouabain (EO), which may be associated with the pathogenesis of asthenospermia by inhibiting the activity of NKA4, affecting Na+/H+ exchange, Na+/Ca2+ exchange and sperm cell membrane potential, and eventually reducing sperm motility.
Asthenozoospermia ; physiopathology ; Humans ; Male ; Membrane Potentials ; Ouabain ; chemistry ; Protein Isoforms ; Sodium-Potassium-Exchanging ATPase ; antagonists & inhibitors ; Sperm Motility ; Sperm Tail ; Spermatozoa ; Testis

Anti-Bacterial Agents ; therapeutic use ; Bacterial Infections ; diagnosis ; therapy ; China ; Communicable Diseases ; diagnosis ; therapy ; Cross Infection ; prevention & control ; therapy ; Granulocyte Colony-Stimulating Factor ; therapeutic use ; Granulocyte-Macrophage Colony-Stimulating Factor ; therapeutic use ; Humans ; Immunoglobulins, Intravenous ; therapeutic use ; Infant, Newborn ; Sepsis ; diagnosis ; therapy

Child ; Chromobacterium ; Female ; Humans ; Sepsis ; drug therapy ; etiology ; microbiology ; mortality

BACKGROUND:Due to the much higher requirement of biocompatibility and anticoagulant of smal-diameter vascular grafts than those of large-diameter ones, in situ blood vessel regeneration occurs as a new research direction. OBJECTIVE:To summarize the recent research development of electrospun smal-diameter scaffolds and to explore the application of in situ blood vessel regeneration and the development tendency. METHODS:The first author retrieved China National Knowledge Infrastructure database, Wanfang data and ISI Web of Knowledge foreign database to retrieve literatures addressing the fabrication of electrospun smal-diameter nanofibrous vascular grafts, surface modification and mimicking extracel ular matrix, as wel as the evaluation of biocompatibility and security after grafting. RESULTS AND CONCLUSION:Electrospun smal-diameter nanofibrous vascular grafts have emerged as promising candidates in vascular tissue engineering. By using both natural and synthetic polymers, the scaffolds can achieve a good balance between mechanical property and biocompatibility. Meanwhile, the fabrication of multi-layered vascular scaffolds, functional surface modification and mimicking extracel ular matrix structural y and functional y are now becoming attractive research directions. However, at current stage, electrospun vascular scaffolds used clinical y are basical y formed by synthetic materials, which have limited biocompatibility and anticoagulant activity. In this case, more efforts should be paid to find an optimal ratio between natural and synthetic materials for the improvement of biocompatibility and anticoagulant ability of smal-diameter vascular grafts.

Objective To investigate the possible correlation between obstructive sleep apnea hypopnea syndrome(OSAHS)and serum epithelial neutrophil-activing peptide-78(ENA-78)and VE-cadherin in pregnant patients.Methods One hundred and two pregnant women combined with OSAHS,42 of which were with hypertension,and 36 healthy pregnant women were underwent polysomnography(PSG),and their apnea hypopnea index(AHI)was recorded.Based on the apnea hypopnea index(AHI),they were divided into mild OSAHS group(36 patients),moderate OSAHS group(34 patients)and severe OSAHS group(32 patients)Serum ENA-78 and VE-cadhefin levels were detected by enzyme-linked immunosorbent assay(ELISA),and mean arterial pressure(MAP)was measured simultaneously.Results The values of AHI,MAP,ENA-78 and VE-cadherin in OSAHS with hypertension were significantly higher than those of OSAHS only group(t =13.46,11.65,12.47,13.36,all P ＜ 0.01)and the control group(t =16.72,15.31,16.35,16.73,all P ＜ 0.01).The levels of serum ENA-78 and VE-cadherin were gradually significantly decreased in the order of severe OSAHS group,moderate OSAHS group and mild OSAHS group(P ＜0.01).The AHI of OSAHS group was significantly correlated with MAP,VE-cadherin,ENA-78(r =0.75,0.71,0.68,all P ＜ 0.01).ENA-78 of OSAHS group were positively correlated with MAP and VE-cadherin(r =0.64,0.67,all P ＜ 0.01).Conclusions The condition OSAHS in pregnant patients is closely related to the levels of ENA-78 and VE-cadherin.Pregnancy combined with OSAHS may take part in the pathological process of hypertensive disorder complicating pregnancy.

The ultramicro-powder dosage is a new type decoction pieces of Chinese medicine.Due to high cell-wall penetrating rate of vegetal cell and increasing of specific surface area,the development of ultramicro-powder dosage has great significance to increasing clinical effects and bioavailability of Chinese medicine.

Objective To evaluate the combination of foot bath using tradirional Chinese medicine and foot massage on sleep quality of patients with insomnia. Methods 84 cases of insomnia patients were divided into the treatment group(44 cases) and the control group(40 cases)according to their willingness whether to accept foot bath and foot massage or not. The control group received routine care, the treatment group was given foot bath using traditional Chinese medicine and foot reflex zone massage at bedtime on the basis of the routine care. The treatment effect were evaluated after 10 days of continuous intervention. Pittsburgh Sleep Quality Index Scale (PSQI)was used to assess sleep quality of patients. Results The treatment efficacy and quality of sleep of the treatment group was significantly better than the control group. After treatment, sleep condition in the treatment group significantly improved than before. ConduMons The combination of foot bath using traditional Chinese medicine and foot massage can improve sleep quality in patients with insomnia.

Objective To explore the etiopathology of slow transit constipation(STC). Methods The model of rat with cathartic colon was established and the changes of colonic electromyography and reaction to acetylcholine(Ach) were examined. Result The frequency, amplitude of slow wave and colon reaction to Ach of rats with cathartic colon decreased markedly. Conclusion Long term abuse of stimulant laxatives could damage enteric nervous system(ENS) and accelerate the pathological changes of STC.

Object To develop a method for the determination of palmatine hydrochloride and berberine hydrochloride in Chinese Mahonia Stem from different habitats Methods HPLC method was set up, using Intersil ODS 3 C 18 column, the mobile phase was acetontrile water sodium laurylsulfonate (470∶ 530∶1 g), the UV detection wavelength was 265 nm, with a flow rate of 1 0 mL/min at 40 ℃ Results A good linearity was obtained in the range of 4 368 52 416 ?g/mL(r=0 999 9) for palmatine hydrochloride and 4 532 54 384 ?g/mL (r=0 999 9) for berberine hydrochloride The average recovery of palmatine hydrochloride and berberine hydrochloride was 98 97% and 98 98%, respectively Conclusion The method is simple, rapid and with better reproducibility for the determination of palmatine hydrochloride and berberine hydrochloride in Chinese Mahonia Stem