1.Respiratory tract microecology and bronchopulmonary dysplasia of preterm infants
International Journal of Pediatrics 2021;48(1):27-30
Bronchopulmonary dysplasia(BPD)is a serious complication of respiratory system in preterm infants.The etiology and pathogenesis are complex and have not been clarified yet.In recent years, studies have shown that there is a certain relationship between respiratory microecology and BPD.Before premature infants develop BPD, their respiratory tract microecology has changed, including abnormal microbial diversity and evolution pattern.Therefore, research on the colonization and evolution of neonatal respiratory tract microecology and its relationship with BPD is expected to provide new ideas for its prevention and treatment.
2.Reprogramming in origin and development of leukemia stem/progenitor cells.
Journal of Experimental Hematology 2009;17(5):1123-1126
The success of yielding induced pluripotent stem (iPS) cells from human somatic cells demonstrates the important role of reprogramming in the formation of stem/progenitor cells and initiates the exploration of the origin of leukemia stem cells. In our previous work, we have found two types of leukemia, bona fide leukemia and non-bona fide leukemia. Different leukemias originate from different leukemia stem/progenitor cells which are critical to the genesis and evolution of leukemia. Bona fide leukemia and non-bona fide leukemia originate from leukemia stem cells and progenitor cells, respectively. Recent research suggests that different types of leukemia are influenced by the reprogramming state of their origin cells.
Cell Differentiation
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Cellular Reprogramming
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Humans
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Induced Pluripotent Stem Cells
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Leukemia
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genetics
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Neoplastic Stem Cells
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Stem Cells
4.Functional multi-polarization of white blood cells and its significance.
Journal of Experimental Hematology 2010;18(1):1-6
Immune and hemopoiesis are one of basic project of experimental hematology. Immune function is a essential activity of white blood cells. It was puzzled for the diversity and complexity of immune response. Polarized immune response of immune cells was discovered 30 years ago, which facilitates the study on differentiation of lymphocyte. Recently recognition on multifunctional polarized immune response of lymphocyte and monocyte/macrophage would promote to elucidate the regulatory network of immune cells, diversity and complexity of immune response as well as the study on hemopoiesis. In this paper the approach of multifunctional polarized immune response of lymphocyte, monocyte/macrophage and dendritic cells were reviewed, and their role, especially in cytokine storm and tumor pro-inflammation condition were discussed.
Cell Differentiation
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Cytokines
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immunology
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Cytotoxicity, Immunologic
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Dendritic Cells
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cytology
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immunology
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Humans
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Monocytes
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cytology
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immunology
5.Comparison between effects of mycophenolate mofetil and rapamycin on proliferation and apoptosis of cyst-lining epithelial cells of ADPKD patients
Tong ZHANG ; Lili FU ; Changlin MEI
Academic Journal of Second Military Medical University 2000;0(08):-
Objective:To investigate the effect of mycophenolate mofetil(MMF)on proliferation and apoptosis of cyst-lining epithelial cells in patients with autosomal dominant polycystic kidney disease(ADPKD),and to compare its effect with that of rapamycin(RAPA)in vitro.Methods: Primary cultured cyst-lining epithelial cells were treated with MMF and RAPA at different concentrations(0,0.005,0.05,0.5,5 ?g/ml)for 48 h or 72 h.The inhibitory effects of them on the cells were evaluated by MTT assay;the cell cycle distribution and apoptotic ratio were determined by flow cytometry.The morphological changes of cyst-lining epithelial cells were observed under transmission electron microscope.Results: Both MMF and RAPA significantly inhibited the proliferation of cyst-lining epithelial cells in a dose-and time-dependent manner.After 48 h treatment,the cells were blocked at S phase by MMF and at G0/G1 phase by RAPA.Both drugs induced cell apoptosis,with the maximal apoptotic rate being(5.53?0.27)% for MMF and(4.36?0.10)% for PAPA.Typical morphological changes of apoptotic cells were observed under electron microscope.Conclusion: MMF can effectively inhibit proliferation and induce apoptosis of cyst-lining epithelial cells,but its inhibitory effect is weaker than that of RAPA.
6.Application of repairing defection by using vascular tissue flap with external fixators in hand surgery
Yongqing ZHUANG ; Xiaokuan FU ; Jing TONG
Chinese Journal of Microsurgery 2000;0(03):-
Objective The purpose is to report the clinical result of repairing defection in hand surgery by using both the vascular tissue flap and external fixators. Methods The vascular tissue flap including bone flap and joint flap to repair the complex defection (soft tissue, bones and joints) in hand and forearm were applied in 9 cases. At the same time in surgery,injured bones and joints were fixed by A-O and mini external fixators in order to keep the non-injured joint actively and accelerate the cure of bones. Result All 9 cases were applied successfully with the all transplanted tissue survived. Pulling about the external fixator about 10- 12 weeks after surgery showed bone union and the f unction of hand recovered satisfactorily' Conclusion it is a reliable method for repairing the complex tissue defection in hand and forearm by combining external fixation of traumarology and microsugery technique. And it is also characterized by a minor injury in transplanted bones,early exercise for joints,flap circulation observed conveniently,and nursing easily.
7.Diagnosis and treatment of severe acute pancreatitis complicated with toxic epidermal necrolysis
Tian FU ; Zhihui TONG ; Weiqin LI
Chinese Journal of Digestive Surgery 2013;12(8):634-636
Toxic epidermal necrolysis (TEN) is an acute skin inflammation,which belongs to heavy drug eruption.With the extensive and widespread use of antibiotics,nonsteroidal antipyretic analgesics and other pharmacotherapies in the treatment of critically ill patients,the trend of morbidity of TEN is gradually increased,especially in intensive care unit.In consideration of the high mortality rate of TEN and its great influence on the overall prognosis of patients with critical illness,we summarized the diagnosis and treatment experiences in the management of TEN based on retrospective analysis of one patient with severe acute pancreatitis and TEN.
8.Apoptosis or necrosis, should which be expected for tumor cells?.
Ke-Fu WU ; Xiao-Tong MA ; Yu-Hua SONG
Journal of Experimental Hematology 2005;13(6):921-923
Evidence has indicated that low doses of anti-tumor regimens can induce cell apoptosis in vitro, although different regimens induce apoptosis by different mechanism and pathway. In recent years, new tumor treatment strategy has been mainly focused on inducing tumor cell apoptosis. The present review discusses the advantages and disadvantages of inducing tumor cell apoptosis. The benefit of inducing apoptosis is not to cause inflammatory reaction, but as its disadvantage, it inhibits immune responses, and the phagocytosis of apopotic bodies may result in horizontal transfer of genes (including oncogenes and other oncogenic materials), which can be one of the causes of tumor relapse. This paper proposes that the tumor treatment strategy should be turn into promoting tumor cell necrosis and inducing anti-tumor immune responses.
Antineoplastic Agents
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therapeutic use
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Apoptosis
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drug effects
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Humans
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Necrosis
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chemically induced
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Neoplasms
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drug therapy
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immunology
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pathology
10.Ala nasal leiomyoma misdiagnosed as nasal vestibular cyst: a case report.
Cui-ping SHE ; Fu-mei MA ; Yi-feng TONG
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2012;47(2):154-154
Child, Preschool
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Cysts
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Diagnostic Errors
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Humans
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Leiomyoma
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diagnosis
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Male
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Nose Neoplasms
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diagnosis
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Vestibular Diseases