This study sought to analyze the conditions of nurses in acute care hospitals struggling with mental health issues. We administered a questionnaire survey to 204 nurses and analyzed 163 valid responses. A total of 81 conditions related to the working environment and 35 conditions related to interpersonal relations were identified. Conditions related to the working environment included working overtime beyond regular workhours, feelings of stress due to assignments, frequency of night shifts, stress in new environments, mistakes made on the job, worries about assigned positions, worries about work procedures, inability to take public and paid holidays, balance between work and raising children, heavy burden of responsibility, loss of confidence, flashbacks to occasions of past stress, trauma, and poor quality of nursing care. Conditions related to interpersonal relations included stress due to pressure from superiors and co-workers, attitude of senior colleagues, stress from senior colleagues, verbal abuse from superiors, behavior of doctors, negative feelings toward patients, changes in department assignments after maternity leave, verbal abuse from co-workers, verbal abuse from senior colleagues, neglect of duties by chief nurses, attitude of juniors, cooperation with other departments, and training of juniors. There were more statements about conditions related to the working environment than to interpersonal relations.