1.A Research on the Anti-tumor Effects of Acid-Soluble Polysaccharides from Gloeostereum incamatum in H22 Tumor Bearing Mice
Hong WANG ; Yan ZHAO ; Enbo CAI ; Tolgor BAU ; Wei LI ; Yu LI ; Haiying BAO
World Science and Technology-Modernization of Traditional Chinese Medicine 2017;19(1):142-148
This study aimed at exploring the inhibitory effect behind its mechanism on acid-soluble polysaccharides from G.incamatum in transplanted H22 tumor mice.Different indices,including tumor inhibitory rate,organ index of liver,thymus and spleen,IL-2,IFN-γ and TNF-α were detected for the evaluation of anti-tumor effects and the mechanism.Furthermore,HE staining and TUNEL assay were adopted to investigate the pathological changes of tumor tissue and cell apoptosis,respectively.As a result,the three dose groups of acidsoluble polysaccharides of G.incamatum successfully inhibited the proliferation of tumor cells,while organ indexes of spleen and thymus were improved and serum IL-2,IFN-γ and TNF-α increased.H&E staining and TUNEL assay showed the polysaccharides induced cell apoptosis,playing a significant role in the inhibition of tumor growth.In conclusion,acid-soluble polysaccharides of G.incamatum possessed significant anti-tumor effects,behind which the mechanism could be related to the regulation of immune regulation,cell apoptosis,and the protection of liver function.
2.Chemical constituents from Lyophyllum decastes.
Hong-Liang ZHENG ; Tolgor BAU ; Hai-Ying BAO ; Jun-Wen LIAN
China Journal of Chinese Materia Medica 2013;38(24):4335-4339
The chemical constituents from the fruiting bodies of Lyophyllum decastes (Fr.) Singer were studied in this paper. Thirteen compounds were isolated and purified by column chromatographies on silica gel and Sephadex LH-20. Their structures were identified by MS and NMR data analysis as adenosine (1), 2R, 3S, 4S, 8E)-2-[(2'R)-2-hydroxyheneicosanoylamino]-8-octadecene-1, 3, 4-triol (2), (2R, 3S, 4S, 8E)-2-[(2'R)-2-hydroxypentacosanoylamino]-8-octadecene-1, 3, 4-triol (3), nicotinic acid (4), (4E, 8E) -2-N-2-hydroxytetracosanoyl-1-O-beta-D-glycopyranosyl-9-methyl-4, 8-sphingadienine (5), D-mannitol (6), ergosteryl-3-O-beta-D-glucopyranoside (7), tuberoside (8), (2R, 3S, 4S, 8E)-2-[(2'R)-2-hydroxybehenoylamino]-8-octadecene-1, 3, 4-triol (9),(2R, 3S, 4S, 8E)-2-[(2'R) -2-hydroxytricosanoylamino] -8-octadecene-1, 3, 4-triol (10), (22E, 24R)-ergosta-7, 22-dien-3beta, 5alpha, 6beta-triol (11), (22E, 24R)-ergosta-5, 7, 22-trien-3beta-ol (12), and 5alpha, 8alpha-epidiory-(22E, 24R)-ergosta-6, 22-dien-3beta-ol (13), respectively. All the above compounds are first obtained from the mushroom and compounds 2-10 are reported to be obtained from the Lyophyllum for the first time.
Agaricales
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chemistry
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Drugs, Chinese Herbal
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chemistry
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Fruit
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chemistry
3.Advance in studies on chemical constituents and pharmacological activity of lichens in Usnea genus.
Laxinamujila ; Hai-Ying BAO ; Tolgor BAU
China Journal of Chinese Materia Medica 2013;38(4):539-545
OBJECTIVETo summarize the studies on chemical constituents and pharmacological activities of lichens of Usnea genus.
METHODA systematic literature survey was conducted to classifiy and summarize chemical constituents of lichens of Usnea genus, and sum up current studies on main pharmacological activities of lichens of the genus.
RESULTLichens of Usnea genus contained multiple chemical constituents, primarily including mono-substituted phenyl rings, depsides, anthraquinones, dibenzofurans, steroids, terpenes, fatty acids and polysaccharides, with such biological activities as antitumor, antibacterial, anti-inflammation, anti-oxidation and antithrombosis.
CONCLUSIONThis essay provides reference for further studies and development of lichens of Usnea genus.
Animals ; Humans ; Medicine, Chinese Traditional ; methods ; Organic Chemicals ; chemistry ; pharmacology ; Usnea ; chemistry
4.Chemical constituents from Usnea longgisima, a traditional mongolian medicine.
Jila LAXINAMU ; Yan-Xia TANG ; Hai-Ying BAO ; Tolgor BAU
China Journal of Chinese Materia Medica 2013;38(13):2125-2128
OBJECTIVETo study the chemical constituents of the whole lichen of Usnea longissima.
METHODThe compounds were separated by silica gel, Sephadex LH-20 chromatography and high performance liquid chromatography (HPLC). The structures of the compounds isolated were identified by physico-chemical properties and spectral analysis.
RESULTTen compounds were isolated and their structures were identified as (4aR,9bS)-2,6-diactyl-3,4a,7,9-tetrahydroxy-8,9b-dimethyl-1-oxo-1,4,4a, 9b-tetrahydrodibenzo [b,d]furan (1), (+)-usnic acid (2), orcinol (3), 18R-hydroxydihydroalloprotolichensterinic acid (4), 5, 8-epidioxy-5alpha, 8alpha-ergosta-6, 22E-dien-3beta-ol (5), ethyl everninate (6), arabitol(7), apigenin 7-O-beta-D-glucuronide (8), 3-hydroxy-5-methoxy-2-methylbenzoic acid(9), friedelin(10).
CONCLUSIONCompound 1 was a new compound. Compound 8 was isolated from genu Usnea for the first time and compounds 3, 4 and 7 were isolated from U. longissima for the first time.
Medicine, Mongolian Traditional ; Usnea ; chemistry