In last years we have produced a combination species from medicinal plants which are used in traditional medicine and has tendentious the increasing of needs and production of plant preparations from natural resources. Although traditional medicine has being increased to be used last years, standardization of them is not sufficient. The standardization is important to ensure the safety, effectiveness and quality of traditional medicine. This research was made with purposes for determining of qualitative and quantitative analyses of biological active substances in the preparation such as Jonsh-5 is utilized to prevent osteoporosis, to accelerate bone ankylose in period of injure. The compositions of the preparation Jonsh-5 are Calcitum, Carduus crispus L. Inula helenium L.Terminalia chebula Retz, Forsutima suspense Thunb. Vahl.In order to succeed the above mentioned aim, we have achieved the following results: 1. The chromatographical method and methodology for the qualitative and quantitative analyses of main compounds in the preparation Jonsh-5 were developed using HPLC. 2. Quantitative analyses of main compounds of the compositions in the preparations were defined by HPLC method. 1.000gr of the preparation Jonsh-5 involves gallic acid 1.11 mg, alantolaktone 1.77 mg.