Tea polyphenols were tried to induce apoptosis of human cultured hepatocellular carcinoma cell line HepG-Ⅱ. MTT assay, DNA agarose gel electrophoresis, transmission electron microscopy , fluorescence decoration and DNA end labeling method (Tunel) were used to identify apoptosis. Having been treated by tea polyphenols in 250μg/ml , HepG-Ⅱcell apoptosis was induced. The induction of apoptosis was the dose dependent. Chromatin condensation, apoptotic body formation, fluorescence of yellow green and pellet were observed. Agarose gel electrophoresis analysis revealed DNA cleavages( DNA ladder). The results indicated that tea polyphenole could induce apoptosis of cultured human hepatocellular carcinoma cell line HepG-Ⅱ. It suggested that tea polyphenols could be used to treat human liver carcinoma.

Tea polyphenols were tried to induce apoptosis of human cultured hepatocellular carcinoma cell line HepG Ⅱ. MTT assay, DNA agarose gel electrophoresis, transmission electron microscopy , fluorescence decoration and DNA end labeling method (Tunel) were used to identify apoptosis. Having been treated by tea polyphenols in 250?g/ml , HepG Ⅱcell apoptosis was induced. The induction of apoptosis was the dose dependent. Chromatin condensation, apoptotic body formation, fluorescence of yellow green and pellet were observed. Agarose gel electrophoresis analysis revealed DNA cleavages( DNA ladder). The results indicated that tea polyphenole could induce apoptosis of cultured human hepatocellular carcinoma cell line HepG Ⅱ. It suggested that tea polyphenols could be used to treat human liver carcinoma.

BACKGROUNDTo investigate the distribution of anti-TTV antibody and the different ORF gene in different populations of China.

METHODSThe antibody to TTV in sera collected from different population were detected by using ELISA and the different ORF genes were amplified with PCR.

RESULTSThe positive rates of TTV ORF1 DNA, ORF2 DNA and the antibody in various populations were as follows: 16.0% (12/75), 10.7% (8/75) and 25.3% (19/75) in paid blood donors; 10.0 (3/30), 16.7% (5/30) and 16.7% (5/30) in patients with hepatitis A, 47.5% (19/40), 42.5% (17/40) and 22.5% (9/40) in patients with hepatitis B; 42.9% (15/35), 37.1% (13/35) and 28.6% (10/35) in patients with hepatitis C; 20.0% (3/15), 26.7% (4/15) and 13.3% (2/15)in patients with hepatitis D; 16.7% (2/12),16.7% (2/12) and 33.3% (4/12)in patients with hepatitis E; 23.8% (5/21), 38.1% (8/21) and 23.8% (5/21) in patients with hepatitis G; 61.1% (11/18), 50.0% (9/18) and 44.4% (8/18) in patients with non A-G hepatitis, respectively. The positive rate of different ORF DNA had no significant difference. Significant differences were found in the positive rates of TTV DNA in various populations. There was no relationship between the TTV DNA and the antibody to TTV.

CONCLUSIONSThe antibody to TTV and TTV DNA were found in every population of China. There was no significant difference in the positive rates of TTV DNA between ORF1 and ORF2. The positive rate in patients with non A-G hepatitis was higher than those in the other populations.

Adolescent ; Adult ; Aged ; Antibodies, Viral ; blood ; China ; epidemiology ; DNA Virus Infections ; epidemiology ; virology ; DNA, Viral ; blood ; Female ; Humans ; Male ; Middle Aged ; Molecular Epidemiology ; Open Reading Frames ; genetics ; Seroepidemiologic Studies ; Torque teno virus ; genetics ; immunology