Objective To observe the effect of electroacupuncture on hand dysfunction after stroke. Methods Forty hemiplegic patients after stroke were selected from a multi-center large-scale trail, belonged to rehabilitation group (n=20) and electroacupuncture group (n=20), who accepted routine rehabilitation and additional electroacupuncture, respectively, for six weeks. They were assessed with Brunnstrom Grade, Fugl-Meyer Assessment (FMA), Wolf Motor Function Test (WMFT) and modified Barthel index (MBI) before treatment, two, four and six weeks during treatment, and twelve weeks after treatment. Results There were differences between two groups in scores of FMA, WMFT and total time to complete WMFT twelve weeks after treatment (t>1.900, P<0.05). There was no difference between two groups in scores of MBI and Brunnstrom Grade (t=0.532, Z<1.79, P>0.05), but electroacupuncture group seemed to improve more. Conclusion Elec-troacupuncture may improve the muscle strength for patients in flaccid paralysis, and promote the recovery of hand function for patients af-ter stroke.

Objective To investigate the change and its mechanism of serum S100? protein and C-reactive protein (CRP) content in patients with acute cerebral infarction(ACI).Methods The content of serum S100? and CRP in 54 patients with ACI and 21 normal control were measured. The changes of S100? and CRP were compared with patients of different pathogenetic condition and infarction sige. The correlation analysis between S100? and CRP was also made.Results The level of serum S100? and CRP in patients with ACI increased significantly compared with control group (all P

Objective To investigate nutrition and health behavior situation and mental situation on puerperal women of Chongqing,in order to provide scientific basis on their pertinent health education. Methods 424 puerperal women were chosen to take health care questionnaire and anxiety self-test table,and investigated by interviewers who had been through professional training,and then doing statistical analysis. Results Women in puerperal in Chongqing had some unreasonable nutrition and health knowledge and mental situation,diet behavior inhibition and so on. Among them,only 49.51% needed calcium supplementation,only 79.37% held that they could have fruit during confinement in childbirth,as high as 66.02% went out after confinement,only 33.49% washed during puerperal,20.39% had over light anxiety. Conclusion It is necessary to reinforce health education and puerperal health guide to puerperal women in Chongqing to improve nutrition and health level and mental situation of puerperal women.

Oral tuberculosis is one of oral infectious diseases and there are some difficulties in its diagnosis.This article describes a case of oral gingival tuberculosis for that the diagnosis had not been confirmed by biopsy.Local injection with streptomycin was undertaken.1 month after treatment the gingival lesion was improved.

Objective To observe the effects of LPS and PMA on proliferation of human len epithelial( HLE )cells and expression of epidermal growth factor receptor(EGFR) in HLE cells.Methods The expressions of EGFR protein of HLE cells from felus,adult lens age-related cataract and cultured HLE cells were detected by immunohistochemical staining.The expression of EGFR mRNA was detected by RT-PCR.The effects of LPS (0.5,1.0,2.0 mg?L-1 ) and PMA(25,50,100 nmol?L-1 )on proliferation of HLE cells were detected by MTT colorimetry method,and the EGFR mRNA expression in HLE cells was determined by RT-PCR. Results The expressions of EGFR protein and mRNA were positive in HLE cells from felus,adult lens age-related cataract and cultured HLE cells.The proliferation rates of HLE cells treated with 0.5,1.0,2.0 mg?L-1 LPS were (3.21?0.42)%,(12.25?1.34)% and (36.67?3.65)%,respectively.The proliferation rate of HLE cells in 2.0 mg?L-1 LPS group was higher than those in 0.5 and 1.0 mg?L-1 LPS groups(F=7.709,P0.05).PMA(25,50,100 nmol?L-1 )could not effect the expression of EGFR mRNA in HLE cells .Conclusion Inflammation stimulant factor such as LPS can promote the proliferation of HLE cells by increasing the expression of EGFR and result in occurrence of posterior capsular opacifition(PCO).

Objective To study the effects of vitamin A deficiency(VAD)and its supplementation on the skeletal growth in rats.Methods Eighteen female Wistar rats were randomly divided into VAD(Vit A 400 IU/kg diet,n=3),supplement(n=9)and control groups(normal diet Vit A 6 500 IU/kg,n=6).The supplement groups were randomly divided into 3 sub-groups,and their diet was changed to Vit A 6 500 IU/kg diet from 14 d after pregnancy,0 d and 4 weeks after birth respectively until they were 7 weeks age old.When they were 0 day,4 and 7 weeks old,their body weight,the length of the tibia,serum Vit A level were measured,and the histology of growth plate was also observed.Results At every measuring times,the serum Vit A level was significantly lower,and the tibial length was shorter in VAD group than in the control(P

The purpose of this study was to study the effect of three different transfection reagents (Lipofectamine™ LTX & PLUS™, Lipofectamine 2000 and Nano-PAMAM-D) and three different testicular injection methods (rete testicular injection, seminiferous tubules injection and testicular interstitial injection) on the efficiency of production transgenic mice. After the mixtures of plasmid DNA (pEFP-C1) and transfection reagent were injected with different testicular injection methods, the sperm density, vitality, positive sperm rates and PCR positive transgenic mice rate were examined 30 days after injection. The results showed that the damage degree from slight to serious of three transfection reagents was Lipofectamine™ LTX & PLUS™, Lipofectamine 2000, and PAMAM-D. The sperm positive rates with green fluorescence of these three groups were 35.65%±0.69%, 12.86%±0.35% and 10.04%±0.20%, respectively. The PCR positive rates of transgenic newborn mice were 29.17%, 13.70% and 5.88%, respectively. Among the groups of different testicular injection methods, the damage degree from slight to serious was rete testicular injection, seminiferous tubules injection, and testicular interstitial injection, whereas the sperm positive rates with green fluorescence were 35.13%, 15.13%, and 0%, respectively. The PCR positive rates of transgenic newborn mice among different testicular injection groups were 33.3%, 12.5%, and 0.0%. The combination of rete testicular injection and Lipofectamine™ LTX & PLUS™ had the lowest toxicity and highest transgenic efficiency in the production of transgenic mice.
Animals ; Humans ; Indicators and Reagents ; chemistry ; Injections ; methods ; Lipids ; chemistry ; Male ; Mice ; Mice, Transgenic ; Plasmids ; Polymerase Chain Reaction ; Seminiferous Tubules ; Spermatozoa ; Testis ; Transfection

Objective:To study the relationship between dietary vitamin A intake and plasma vitamin A concentration , and establish the theoretical basis for dietary intake predicting vitamin A nutritional status . Methods:By using cluster sampling , 492 children aged 2-7 years in kindergartens in Banan district of Chongqing were selected .A cross-sectional nutrition and health survey was conducted , including the clinical examination , anthropometry , laboratory test and dietary survey .Results: Among the children surveyed, 229 were boys, and 263 girls, the mean age was (4.54 ±0.87) years, height (107.50 ± 7.20) cm, and weight (18.42 ±3.41) kg, the mean value of plasma vitamin A was (1.04 ±0.30)μmol/L.The prevalence of marginal vitamin A deficiency ( MVAD) was 43.5%.No cases of severe clinical vitamin A deficiency were found (plasma vitamin A≤0.35μmol/L).Clinical examination found no conjunctiva, corneaor skin abnormalities, and no Bitot’s spots.Prevalence of the last two weeks colds were 27.4% (135/492), no diarrhea and other gastrointestinal or digestive diseases were found .The proportion of insufficient dietary vitamin A intake ( <600 μg RE/d) was as high as 50.0%.By using correlation analysis, plasma retinol concentrations were related to dietary vitamin A intake (r=0.162, P<0.001), and to dietary energy intake (r=0.107, P=0.017).After adjustment for the effects of other non-dietary factors on vitamin A deficiency , the multivariate logistic regression showed that vitamin A-rich foods of liver intake=0 g/d (OR=1.95, 95% CI:1.05-3.61, P=0.034), vitamin A-rich fruits intake=0 g/d (OR =1.55, 95% CI: 1.03 -2.33, P =0.034), vitamin A-rich vegetables intake<200 g/d (OR=3.47, 95%CI:1.37-8.75, P=0.009) were important risk factors of vitamin A deficiency .But we had not found the correlation between the intake of meat , eggs and milk and vita-min A deficiency .Conclusion:Dietary factors may be the major risk factor of vitamin A deficiency in the three kindergartens .The dietary vitamin A intakes are significantly related to plasma retinol concentra-tions, and the vitamin A-rich foods intakes can predict the body ’ s vitamin A nutritional status .

BACKGROUND:Which an ideal method to treat type Rockwood Ⅲ acromioclavicular joint dislocation is,traditional or surgical therapy,is still controversial.OBJECTIVE:To investigate the clinical effect of clavicular hook plate implantation on type Rockwood Ⅲ acromioclayicular joint dislocation.METHODS:A total of 56 patients with type Rockwood Ⅲ acromioclavicular joint dislocation were selected from Department of Orthopaedics of Yiling Hospital between December 2005 and June 2008.There were 42 males and 14 females,aged 16-65 years and mean age of 32 years.All patients were treated with clavicular hook plate implantation.Indicators including visual analogue scale (VAS),the United States shoulder and elbow surgeon score (ASES),and Constant and Murley scoring system were tested pre-operatively,one year postoperatively,before internal fixation,and 3 months after internal fixation;additionally,functional changes of the shoulder joint and complications were analyzed before and after clavicular hook plate implantation.RESULTS AND CONCLUSION:The surgery and following-up were successfully achieved in 56 cases.The following-up lasted for 15-30 months,with the mean time of 20 months.The internal fixation was taken out at about 1 year after implantation.Coracoclavicular ligament was repaired in 32 cases but not in the 24 cases.Two patients with acromioclavicular joint dislocation recurrence were excluded,and coracoclavicular ligament therapy was not performed.Shoulder pain,foreign body sensation,and internal fixation failure occurred in two cases.One year after operation,VAS was decreased compared with that before operation,but ASES and Constant and Murley score were significantly increased (P＜0.01).At three months after internal fixation,VAS was decreased compared with that at 1 year after operation,but ASES and Constant and Murley score were increased (P＜0.05).The results demonstrated that clavicular hook plate implantation for treating type Rockwood Ⅲ acromioclavicular joint dislocation is simple and less invasive,thus it is an ideal internal fixation.

Objective To assess the predictive value of short-latency somatosensory evoked potential (SSEP) in the acute phase of stroke regarding functional recovery. Methods One hundred and fifty stroke patients were included. SSEPs were recorded on the first 7 days after stroke, and the patients were then stratified into groups with absent, abnormal and normal responses. Clinical state was determined according to the NIH stroke scale (NIHSS), the Fugl-Meyer assessment (FMA), the modified Barthel index (MBI) and the modified Rankin scale (MRS). SSEP was followed up at 1, 3, 6 and 12 months. Results The NIHSS and FMA scores were significantly different comparing any two groups or at different times in the same group. The median SSEP N20 latency and tibial nerve SSEP P40 latency during stroke were positively correlated with MRS scores 12 months later but negatively correlated with MBI results. The ADL results 12 months after stroke were used to evaluate functional recovery, and the prediction rate of the SSEP results alone was 40.8% , however the rate improved to 44.2% when SSEP results were combined with FMA scores and became 46.1% when SSEP, FMA and MRS results were all considered. Conclusion SSEP has independent predictive value regarding functional recovery after stroke. Combined assessment of initial FMA, MRS and SSEP substantially improves the predictive power for stroke prognosis.