To explore the effectiveness of positron emission tomography (PET) respiratory gating based on time and amplitude to improve the reconstructed image artifacts. It is necessary to appropriately select acquisition and processing methods which ensure the readability of myocardial imaging images in radionuclide medicine myocardial imaging. This summary mainly discusses the identification method and how to avoid adverse effect for images from myocardial imaging process, and includes similarities and differences of varieties methods: identification for image artifacts of myocardial imaging, image reconstruction algorithms and software processing and so on. It should be significant to choose the appropriate method to identify myocardial imaging artifacts in image collection. The pros and cons of myocardial imaging image reconstruction algorithm and various software processing methods still remain unknown. How to choose better appropriate acquisition, image reconstruction and software processing methods for myocardial imaging need further study.

Heme oxygenase-1(HO-1) is an inducible stress protein.In addition to its role in heme degradation,several positive biological effects exerted by this enzyme have highlighted in recent years as anti-inflammatory,anti-apoptotic,anti-proliferative and cytoprotective functions.This oxidase system with multiple functions takes part in the pathophysiology of cardiovascular system.It is related to a variety of cardiovascular diseases including hypertension,atherosclerosis,ischemia/reperfusion injury and postangioplasty restenosis.

Purpose]To explore the paste treated postpartum body pain in curative effect. [Methods]By fol owing professor Zhang diagnosis ,the paper summarized with herbal paste for gynecology as the methods of treatment of postpartum body pain, and listed 2 proven cases.[Result]Postpartum woman is weak in both qi and blood, paste has an advantage in taking the fast-rising tonifying deficiency and curing correction,and it is superior to the liquid form, convenient for long-term use, paste treating pantalgia after childbirth in the disease has a unique advantage, and because of its smal side effects, especial y suitable for women. [Conclusion]Herbal paste in chronic disease of department of gynaecology modulation has a unique advantage due to the efficacy of comprehensive,and takes into consideration the physiological and pathological features of menstruation,leucorrhea,pregnancy and delivery. She focuses on the individual syndrome differentiation,and attaches great importance to the balances of relationship between pathogenic factors and healthy qi,yin yang heat cold,viscera and meridians qi blood,to achieve the aim of preventing and treating diseases,regulating the body ,and is worth promoting.

As a platform to learn authentic patient experiences, learning health system(LHS)acquires big data from clinical environment and other health-related events, analyzes data to produce new knowledge and applies these knowledge to constantly guide clinical decision and practice, thus contributing effectively to better quality of care and lower medical cost. The authors reviewed LHS in terms of its role model, organizational structure, infrastructure framework, core characteristic elements framework and researcher′s core competence framework. Based on the study, the authors recommended the integration of multi-source heterogeneous medical information platform data, and enhancement of synergy among medical institutions and multi-disciplinary teams in the introduction and localization of LHS into China.

Objective To investigate the clinical significance of lipoprotein-associated phospholipase A2(LP-PLA2) measure-ment for type 2 diabetes mellitus(T2DM) patients combined with atherosclerosis .Methods The concentrations of LP-PLA2 ,cho-lesterol(CHO) ,triglyceride (TG) ,low density lipoprotein(LDL) ,high density lipoprotein(HDL) were measured for 47 T2DM pa-tients combined with atherosclerosis(patient group) and 94 healthy people who just underwent healthy examination and were proved to be healthy(control group) ,meanwhile the age and gender information of those people were collected .Then the tests results were compared between the two groups .Results There was no significant difference in age ,gender ,CHO ,HDL ,TG ,LDL between the two patient and control group(P>0 .05);Plasma LP-PLA2 level in patient group was higher than that in control group ,and the difference was statistically significant(P<0 .05) .Non-conditional Logistic regression analysis showed that the LP-PLA2 level was the risk factor with statistical significance(P<0 .05) .Conclusion LP-PLA2 concentration is an independent risk factor for athero-sclerosis in T2DM patients ,and its measurement has certain value for the diagnosis of atherosclerosis in T2DM patients .

Objective To evaluate the feasibility of 80 kV tube kilovoltage in aortic artery CT angiography with 256-slice CT. Methods A total of 62 patients undergoing aortic artery CTA were enrolled into this study and divided into conventional tube kilovoltage (120 kV, n = 31 ) and low tube kilovoltage (80 kV, n = 31 ). The signal-to-noise ratio (SNR) , contrast-to-noise ratio (CNR), volume CT dose index (CTDIvol) and effective dose (E) were evaluated, respectively. Results The mean image SNR was ( 35.92 ± 5.04) and ( 33.95 ± 8. 30) for conventional tube kilovoltage and low tube kilovoltage,respectively, with no significant difference (t = 1. 131, P =0. 263). The mean image CNR was (30. 32 ±4.78) and (28.71 ± 7.96 ) for conventional tube kilovoltage and low tube kilovoltage, respectively, with no significant difference ( t = 0. 964, P = 0. 339 ). The average effective dose ( E ) was ( 14. 28 ± 0.96 )mSv and (9. 72± 0. 81 )mSv for conventional tube kilovoltage and low tube kilovoltage, respectively, with significant difference ( t = 20. 12, P ＜ 0. 001 ). Conclusions 80 kV tube kilovoltage aortic artery CTA can reduce radiation dose by 31.9% , and contrast dose 50% ,and maintain image quality compared with 120 kV tube kilovoltage.

Objective To explore the effects of bone marrow mesenchymal stem cells (BMSCs) on viral myositis in mice. Methods Four-week-old BALB/C male mice were randomly divided into normal control group, myocarditis group, and BMSCs intervention group at different stages (3 days and 2 weeks). The mouse model of viral myocarditis was established by intraperitoneal injection of Coxsackie virus B3. The mice in the intervention group were injected with BMSCs in the tail vein at 3 days and 2nd week after the injection of the virus. Four weeks later, echocardiography was performed, and the pathological integral and collagen volume fraction (CVF) were observed and calculated by light microscopy. The qRT-PCR method was used to detect the mRNA expression of homogenates collagen I (col1α1) and collagen fiber III (col3α1) in myocardial tissue. Results Compared with the normal control group, the left anterior and posterior wall became thinner, the diameter and volume of the left ventricle at end systolic period was increased; left ventricular ejection fraction (LVEF) and short axis shortening rate (FS) decreased in the myocarditis group. The differences were statistically significant (P all<0.05). The LVEF and FS in each subgroup of the intervention group were better than those of the myocarditis group, and the improvement in the intervention group was more obvious at the 2nd week after the treatment of the myocarditis. The differences were significant (P all<0.05). Light microscope showed that myocardial CVF in myocarditis group was higher than in normal control group, and CVF in intervention group was reduced compared with myocarditis group and CVF in the 2nd week intervention group was lower than that in the 3 day intervention group. The differences were significant (P all<0.05). Compared with the control group, the mRNA expressions of col1α1 and col3α1 in the myocarditis group were increased, and they were lower in the intervention group than in the myocarditis group, and the differences were significant (P all<0.05). Conclusions BMSCs can reduce the degree of cardiac fibrosis and improve cardiac function in mice with viral myositis, and the intervention effect is better when the virus is infected in the 2nd week.

Objective To investigate the efficacy and safety spinal ropivacaine and fentanyl-epidural anes-thesia for the first stage of preclinical of childbirth.Methods 78 cases of full-term maternal were divided into the three groups randomly by using digital representation,each group had 26 cases.A group did not implement analgesia, B group was given analgesia in the first stage of incubation(ropivacaine and fentanyl epidural anesthesia),C group was given labor analgesia on the first line of the active phase.The labor time,mode of delivery,Apgar score,pain VAS score and the occurrence of adverse reactions of three groups were observed.Results The VAS pain scores of B group were (5.01 ±0.61)points,(2.30 ±0.32) points and (1.65 ±0.21) points after 5,10,30min of analgesia, which were significantly lower than those of before(t=6.59,9.62,10.73,all P<0.05) and A group(t=6.76, 9.80,11.02,all P<0.05);The VAS pain scores of C group were (4.92 ±0.57)points,(2.34 ±0.30)points and (1.61 ±0.23)points after 5,10,30min of analgesia,which were significantly lower than those of before(t=9.72, 9.55,10.91,all P<0.05) and A group( t=6.88,9.73,11.30,all P<0.05);The VAS pain scores of B group and C group showed no significant difference(t=2.22,2.08,1.99,all P>0.05) at each time point;The first stage of labor of B group and C group were (327.43 ±31.02)min and (335.62 ±30.18)min,respectively,which were significantly decreased compared with A group(t=7.21,6.90,all P<0.05);The natural birth rate of B group and C group was 80.77%and 84.62%,respectively,which were significantly higher than 57.69%of A group(χ2 =8.43, 8.81,all P<0.05);There was no significantly different among three groups in the incidence of adverse reactions (χ2 =2.23,2.23,all P>0.05).Conclusion Ropivacaine and fentanyl in epidural anesthesia had a preferable effect for the first stage of preclinical and active period of childbirth which can effectively reduce maternal labor pain and improve the natural birth rate and has a fewer adverse reactions.

Objective To evaluate the feasibility of automatic tube current modulation(ATCM) in craniocervical artery CT angiography with 256-slice CT,while maintaining the image quality.Methods Sixty patients undergoing craniocervical artery CTA were enrolled into this study,and were divided into conventional tube current group(250 mAs,group A,n=30) and low tube current group(ATCM,group B,n=30).The signal-to-noise ratio(SNR),contrast-to noise ratio(CNR),volume CT dose index(CTDIvol) and effective dose(ED) were evaluated respectively.Results The mean image SNR was(37.47?9.39) and(36.85?7.96) for group A and group B respectively,and was not significantly different(P=0.79).The mean image CNR was(32.13?9.06) and(31.86?7.66) for group A and group B respectively,and was not significantly different(P=0.90).The CT volume dose index(CTDI) was 16.5 mGy and 410.55?0.94mGy for group A and group B respectively,and was significantly different(P

Objective To explore the expression levels of microRNA(miR)-34a in hippocampus of temporal lobe epilepsy rats and the effect of miR-34a on its target signaling pathway Notch1.Methods Rats were divided randomly into experiment group (n=40) and control group (n=40) by adopting random number table method.The status epilepticus model and the temporal lobe epilepsy model were induced by using lithium-pilocarpine for experiment group.The control group rats received an injection of an equal amount of 9 g/L saline as instead of pilocarpine.Racine grading was performed at 24 hours,day 3,day 7,day 15,and 1 month after modeling to evaluate the behavior.Real-time fluorescent quantitative polymerase chain reaction was used to test the mRNA expressions of miR-34a and Notch1.Western blot was performed to explore the protein expression of Notch1.Results The expression levels of miR-34a at post-status epilepticus in 24 hours,day 3,day 7,day 15 were 2.55±0.29,2.11±0.17,1.68±0.49 and 1.84±0.42,respectively,which showed statistically significant difference compared with the control group (1.00±0.00) (t=-1.55,-1.11,-0.68,-0.84,all P<0.05).The expression levels of Notch1 mRNA at post-status epilepticus in 24 hours,day 3,day 7,day 15,1 month were 1.44±0.31,1.27±0.13,1.52±0.28,0.91±0.33,and 0.80±0.09 respectively.There were significant differences at 24 hours,day 7 in Notch1 mRNA expression (t=-0.44,-0.52,all P<0.05) compared with the control group(1.00±0.00).The expression level of Notch1 mRNA on day 15 was significantly lower than 24 hours and day 7 (t=-0.54,-0.62,all P<0.05),and the expression in 1 month was significantly lower than in 24 hours,or day 3 and day 7 (t=-0.64,-0.46,-0.72,all P<0.05).The expression levels of Notch1 protein at post-status epilepticus 24 hours,day 3,day 7,day 15,1 month were 0.78±0.09,0.57±0.13,0.55±0.16,0.42±0.13,and 0.33±0.09,respectively.There was significantly up-regulated at 24 hours of Notch1 protein expression compared with control group (0.51±0.15)(t=-0.20,P<0.05);and the expression level at day 15 were significantly lower than 24 hours (t=-0.26,P<0.05),while the expression in 1 month was significantly lower than in 24 hours and on day 3 (t=-0.36,-0.24,all P<0.05).Conclusion miR-34a is significantly up-regulated in the post-status epilepticus rat hippocampus,and it may contribute to temporal lobe epilepsy by activating Notch1 signaling pathway.