Objective To evaluate the effects of soybean protein and animal protein on renal function of patients with diabetic nephropathy.Methods In this Meta-analysis,literatures on the effects of soybean protein and animal protein on renal function of patients with diabetic nephropathy published during January 1980 and November 2011 were reviewed and analyzed quantitatively and synthetically by using Metaanalysis RevMan 5.0 software.Results Five case-control studies met the inclusive criteria and entered into random effect model.In comparison with animal protein,soybean protein significantly improved endogenous creatinine clearance rate (weight mean difference (WMD) =-11.51,95％ confidence interval (CI)-16.37 to 6.65),proteinuria (WMD =-0.96,95％ CI-1.36 to-0.58),urine creatinine (WMD =-1.11,95％ CI-1.91 to-0.30),glomerular filtration rate (WMD =0.28,95％ CI-0.12 to 0.68),serum urea nitrogen (WMD =-0.20,95％ CI-0.60 to 0.19) and serum creatinine (WMD =-0.22,95％ CI-0.54 to 0.10).Conclusions Soy protein seems to have potential to improve urine creatinine clearance,urine creatinine,proteinuria and serum urea nitrogen.However,animal protein may contribute to increased glomerular filtration rate.

Metallothionein is a sulfhydryl-rich,and chelating a large number of metal ions,which is widely found in biological world.It is very highlighted that MT had the same physical and chemical features from different origins.Plenty of research data indicated that MTs contain 20 free sulfhydryl genes,as well as their sulfhydryl clusters,It is a multifunctional protein in health.Many experiments demonstrate that MTs may be a novel target of original new drugs because they play a role in regulating and controlling the organ fibrosis and tumor.

Objective To evaluate the effects of propofol combined with resveratrol on hepatic ischemiareperfusion (I/R) injury in rats.Methods One hundred and eight male Sprague-Dawley rats,aged 6-7 weeks,weighing 220-270 g,were randomly divided into 6 groups (n =18 each):sham operation group (group S) ; I/R group; solvent group (group TW-80) ; propofol group (group P) ; resveratrol group (group R) ; propofol combined with resveratrol group (group P + R).The animals were anesthetized with intraperitoneal 10 ％ chloral hydrate 3.5-4.0 ml/kg.Liver ischemia was produced by clamping the first hepatic portal for 30 min,followed by 12 h reperfusion.In group P,propofol was infused intravenously at 10 mg· kg-1 ·h-1 starting from 10 min before ischemia until the end of operation.In group R,resveratrol 10 mg/kg was injected intravenously at 10 min before ischemia.In group P + R,resveratrol 10 mg/kg was injected intravenously and then propofol was infused intravenously at 10 mg· kg-1· h-1 starting from 10 min before ischemia until the end of operation.The equal volume of normal saline was given instead in groups S and I/R,and the equal volume of TW-80 was given instead in group TW-80.Six rats in each group were chosen at 3,6 and 12 h of reperfusion and blood samples were taken from the superior and inferior vena cava for measurement of serum alanine aminotransferase (ALT) and aspartate amino transferase (AST) activities.The rats were then sacrificed and livers were removed for microscopic examination and for determination of superoxide dismutase (SOD) and myeloperoxidase (MPO) activities,inducible nitric oxide synthase (iNOS) expression,and malondialdehyde (MDA) and nitric oxide (NO) contents in liver tissues.Results Compared with group S,the serum ALT and AST activities,and MDA and NO contents,MPO activity and iNOS expression in liver tissues were significantly increased,and the activities of SOD were decreased in the other four groups (P ＜0.05).Compared with group I/R,the serum ALT and AST activities,and MDA and NO contents,MPO activity and iNOS expression in liver tissues were significantly decreased,and the activities of SOD were increased in groups P,R and P + R (P ＜ 0.05).Compared with groups P and R,SOD activity was significantly increased,and the other parameters were decreased in group P + R (P ＜ 0.05).The pathological changes were significantly attenuated in groups P,R and P + R compared with group I/R.Conclusion Pretreatment with propofol and resveratrol can attenuate hepatic I/R injury in rats by increasing antioxidation and inhibiting lipid peroxidation and reducing production of endogenous NO,and the combination of the two agents provides better efficacy than etheir alone.

10.3969/j.issn.1007-3969.2013.04.009

OBJECTIVE To investigate the protective effect of total alkaloids of Rhizoma Corydalis (TARC) on experimental gastric mucosal lesions in rats. METHODS Gastric mucosal lesions were induced in rats by injecting acetic acid under gastric mucosal. From the 2nd day post the preparation of the rat model, cimetidine 400 mg · kg-1 or TARC 20, 40 and 80 mg · kg-1 was ig delivered for 15 d in different groups. Two days after the last delivery, gastric juice volume and total acidity were measured. Histopathology of stomach tissues was observed by HE staining. The area of gastric ulcer area was measured and the ulcer index and ulcer inhibitory rate were calculated. The expression of epidermal growth factor (EGF) and EGF receptor (EGFR) was detected by immunohistochemical staining. RESULTS Comparing with shame group, the eare of gastric ulcer and ulcer index were increased signifi?cantly in the model group(P<0.01), suggesting that the model rats were prepared properly. Compared with the model group, the ulcer area in rats of cimetidine and TARC 80 mg·kg-1 groups was decreased by 39.9%and 23.7%,respectively. The ulcer index was decreased by 52.3%and 30.5%,respectively. There was no significant difference between the cimetidine group and TARC 80 mg · kg-1 group, in the ulcer area or index. Compared with model group, EGF protein expression of cimetidine and TARC 40 and 80 mg · kg-1 groups was increased by 81.8%,24.2%and 57.6%,respectively while EGFR protein expression was increased by 45.9%,16.2%and 29.7%,respectively(P<0.05,P<0.01). Deciduous and necrotic gastric mucosal and a large amount of inflmmatory cells infiltration were observed in model group, and gastric mucosal lesions were improved in cimetidine and TARC 40 and 80 mg · kg-1 groups. CONCLUSION TARC has protective effect on gastric mucosal lesions in rats. The mechanism may be related to the increase of EGF and EGFR protein expression.

Objective To quantify the concentration of peripheral blood plasma neutrophil gelatinase-associated lipocalin (NGAL) in patients with acute pancreatitis (AP) and to explore its value in assessment of the severity of AP.Methods From June 2011 to March 2012,83 patients with AP were selected,among those 43 cases were mild acute pancreatitis (MAP) and 40 were severe acute pancreatitis (SAP).The control group included 30 healthy individuals.The peripheral blood of patients with AP and healthy controls was collected,and plasma was isolated after centrifuged.The concentration of NGAL in plasma was detected by enzyme-linked immunosorbent assay (ELISA).The t-test was performed for comparison between groups.The correlation between the concentration of NGAL in plasma and clinical parameters of AP was analyzed by Spearman rank order correlation analysis.The diagnosis value of the concentration of NGAL in SAP was evaluated by receiver operating characteristic (ROC) curve and area under the curve (AUC).Results The concentration of plasma NGAL in AP group ((10.30± 5.97)nmol/L) was higher than that in healthy control group ((1.94±1.35) nmol/L) and the difference was statistically significant (t=11.924,P＜0.01).The concentration of plasma NGAL in SAP group ((14.61 ±5.28) nmol/L) was higher than that in MAP group ((6.27±-3.09) nmol/L) and healthy control group,the differences was statistically significant (t=8.677 and 14.539,both P＜0.01).The concentration of plasma NGAL of AP patients was positively correlated with acute physiology and chronic health evaluation-Ⅱ score,Ranson score,bedside index for severity in acute pancreatitis score,computed tomography (CT) severity index,C-reactive protein,white blood cells and the days of hospitalization (r=0.651,0.556,0.514,0.620,0.320,0.458 and 0.346,all P＜0.05).The area under the ROC curve of plasma NGAL concentration in diagnosis of SAP was 0.926 (95％CI:0,870-0.983).The cutoff value of plasma NGAL level in diagnosis of SAP was 8.44 nmol/L.The sensitivity and specificity was 87.5 ％ and 88.9％,respectively.Conclusions Plasma NGAL level is correlated with the severity of patients with AP.NGAL may be one of the markers for the early diagnosis of SAP.

Objectives To explore the effect of mibefradil, a kind of novel calcium channel antagonists, on proliferation of human pulmonary artery smooth muscle cells (HPASMCs) induced by platelet-derived growth factor (PDGF). MethodsHPASMCs were culturedin vitro, and randomly divided into control group, PDGF group, Mib group, and PDGF+Mib group. The PDGF group was stimulated by 25 ng/ml of PDGF. Mib group was intervented by 10 μmol/L of Mib. PDGF+Mib group was treated by PDGF and Mib. The reproduction rate in 48 hours and 72 hours were detected by MMT. Cell cycle was detected by lfow cytometry. The expression of proliferating cell nuclear antigen (PCNA) was observed by immunolfuorescence staining (IFS).ResultsThere were statistical differences among four groups in both 48 hours and 72 hours (P all??0.05). There were statistically differences of G0/G1 phase and S phase among four groups (P?

Objectives To explore the clinical features and pathogenesis of Kawasaki disease (KD) combined with sterile pyuria. Methods A total of 420 patients diagnosed of KD were recruited and divided into pyuria group ( 95 patients) and control group ( 325 patients) according to urine routine examination on admission. The clinical data between the two groups were compared. Results There was no difference in gender, age, and the incidence of atypical KD (P all?>?0 . 05 ). The levels of C-reactive protein, D-dimer concentrations, fibrinogen degradation products, alanine aminotransferase, aspartate aminotransferase, and urine retinol binding protein were higher in pyuria group than those in control group (all P>?0 . 05 ). No difference was found in the duration of fever before admission between two groups (P>?0 . 05 ). However, pyuria group had longer duration of fever after treatment with immunoglobulin (P?0.05). Conclusion The morbidity of sterile pyuria in KD patients was 22 . 6%. KD patients with sterile pyuria have more intense inlfammatory response, markedly high coagulation condition, and mild or subclinical renal damage.

Objective:To investigate the effects of Jianpijiedutang combined with Kangfuxin Liquid in the treatment of recurrent oral ulcer(ROU).Methods:80 patients with ROU were divided into 2 groups(n=40).The patients in control group were treated with Kangfuxin Liquid local spray,those in the test group were treated with Jianpijiedutang combined with Kangfuxin Liquid spray.The patients were followed up for 24 weeks.The effects were evaluated according to the trial standard of Chinese Stomatological Association.Results:The interval of ROU increased and ulcer number decreased in the 2 groups during the observation period,the test group showed more effective outcome than the control group(P<0.05).In the treatment group ROU interval increased more than in the control group(P<0.01),and ROU ulcer number decreased more(P<0.01).Conclusion:The Jianpijiedutang combined with Kangfuxin Liquid spray is more effective than the latter used only.

Objective To compare the duration of neuromuscular blockade in laparoscopic and open surgical approaches following a single bolus dose of rocuronium or cisatracurium.Methods One hundred female patients underwent either laparoscopic or open gynaecological surgery were randomly and equally divided into OR group (rocuronium administered in open surgery),OC group (cisatracurium administered in open surgery),LR group (rocuronium administered in laparoscopic surgery) and LC group (cisatracurium administered in laparoscopic surgery).Anaesthesia was induced with sufentanil of 0.5 μg/kg and propofol of 2 mg/kg,and neuromuscular blockade was induced with rocuronium of 0.9 mg/kg or cisatracurium of 0.15 mg/kg,intravenously.Then adductor pollicis trainof-four responses following ulnar nerve stimulation were monitored with mechanomyography.We recorded the duration of the first twitch (T1) occurrence after the injection of rocuronium or cisatracurium,and the duration from T1 recovered to 5 ％ and 25 ％ of baseline.Results The mean time from the end of injection of rocuronium until spontaneous recovery to T1,and to recovery to 5％and 25％ of baseline,was significantly prolonged in LR group [(44.8 ± 10.7) min,(52.8 ± 11.2) min and (62.6 ± 13.5) min] compared with OR group [(36.2 ± 4.0) min,(41.8 ± 6.8) min and (49.5 ±7.5) min] (P all＜0.05) On the other hand,there was no significant difference on the duration of neuromuscular blockade between LC group and OC group.Conclusions Neuromuscular blockade may be prolonged following a single bolus dose of rocuronium given during laparoscopic procedures,which should be on the alert,and assess neuromuscular function to ensure safe recovery.