Objective To observe relationship between the phlegm and adhesion molecule and further explore the functions of the phlegm in the metastatic potential of the tumors. Methods Examin the expression difference of ICAM-1,E-cad,MMP-9 of gastric cancer by enzyme linked immunosorbent assay between the phlegm-stamp type and non-phlegm-stamp type,then reducing phlegm by chinese medicine treatment and comparing theirs expression variation.Results The expressions of ICAM-1,E-cad, MMP-9 were obviouslv different between the phlegm group and non-phlegm group[(403.6±99.7)μg/L,(9.08±1.69)mg/L,(465.0±96.64)μg/L &(319.9±81.4)μg/L,(7.56±1.15)mg/L,(228.1±43.79)μg/L].After using xiao-tan-san-jie recipes,the expressions of ICAM-1 and E-cad of the phlegm group were obviously lower than before(P<0.05);the expression of MMP-9 was also falling down,but the difference was not significative.Conclusion There is a correlation between the phlegm and adhesion molecule, which could take action in tumor metastams by affecting adhesion molecule expression.

Objective To observe the relationship between plasma level of homocysteine(HCY) and ischemic stroke in young and middle-aged adults,explore the clinical signficance concerning the occurrence and development of ischemic stroke in young and middle-aged adults.Methods The plasma homocysteine level of 132 young and middle-aged adults patients with ischemic and 86 control peoples were measured by means of enzymatic cycling assay.Results The plasma homocysteine level in ischemic stroke was higher than that in control group( P ＜ 0.01 ).The higher the plasma homocysteine level,the larger infarcted focus in acute ischemic stroke group of young and middleaged adults.The plasma homocysteine level and the infarcted focus was positively linearly correlated.Conclusion Hyperhomocysteinemia is a risk factor of ischemic stroke among young and middle-aged adults.The higher the plasma homocysteine level,the larger infarcted focus in acute ischemic stroke group.The plasma homocysteine level can reflect the size of the infarcted focus and the degree of disease,and the plasma homocysteine level should serve as a kind of regular examination or as an index intervention can be taken,all of which are great importance to prevent ischemic stroke and reduce its morbilily.

OBJECTIVETo analyze the distribution of chemical constituents in different parts of Changium smyrnioides.

METHODThe content of polysaccharides, mannitol and choline was determined by colorimetry and the water-soluble components was determined by HPLC. similarity coefficient of water-soluble components was calculated by the included angle cosine method.

RESULTThe Contents of chemical constituents were significantly different between the leaves and the roots. The contents of choline and polysaccharides in the roots was higher than those in the leaves, but the contents of water-soluble extract and physiological ash in the leaves was higher than those in the roots. Contents of chemical constituents were also significantly different at different segments in the root, which decreased from the upper to the lower parts, increased from the outside to the inside parts except physiological ash, and the difference in horizontal segments was higher than that in vertical segments. Total peak area of water-soluble components in the leaves was 2.81 times higher than that in the roots, and gradually increased from the outside to the inside parts.

CONCLUSIONTotal content of chemical constituents in the leaves was significantly higher than that in the roots. The quality of medicinal used parts decreased gradually from the inside to the outside parts and from the upper to the lower parts in the root of Ch. smyrnioides.

Apiaceae ; chemistry ; growth & development ; Chromatography, High Pressure Liquid ; Plant Leaves ; chemistry ; Plant Roots ; chemistry

OBJECTIVETo analyze the dynamic change of chemical constituents in Changium smyrnioides at different ages and give a reference for standard cultivation.

METHODThe contents of polysaccharides, mannitol and choline in the roots were determined by colorimetry. HPLC was used to analyze water-soluble components in the roots. GC-MS was used to analyze volatile oil components in the leaves. Similarity coefficient of water-soluble components was calculated by included angle cosine method.

RESULTThere was significant difference in content of the analyzed chemical constituents except polysaccharide. Contents of mannitol, physiological ash and polysaccharide in the adult plant were higher than those in the young plant. On the contrast, content of water-soluble components and choline in the young plant was higher than that in the adult plant. With the increase of the growth years, water-soluble components in the roots and volatile oil components in the leaves were gradually concentrated.

CONCLUSIONThe growth time significantly affects the chemical constituents in Ch. smyrnioides, the reasonable harvest time is from the second to the forth year after transplanting.

Apiaceae ; chemistry ; growth & development ; Plant Extracts ; analysis ; Plant Leaves ; chemistry ; growth & development

OBJECTIVETo analyze water-soluble components in the roots of Ch. smyrnioides among different populations that distributed in the main areas and give a reference for germplasm evaluation and quality control.

METHODWater-soluble components were extracted with the cold-soaking method and analyzed by HPLC, similarity coefficient was calculated by included angle cosine method according to relative content of major water-soluble components, and systematic relationships were constructed based on UPGMA method.

RESULTThere was significant difference in water-soluble components in root among population. Jiuhuashan population had the highest content of water-soluble extract. The content of water-soluble extract was below the pharmacopoeia standard in the root of Dalongshan population and Fushan population. There was significant difference in the HPLC chromatogram of water-soluble components in the root of Ch. smyrnioides from different populations, and the number of common peak was small. Similarity coefficient significantly ranged from 0.0306 to 0.9995 among 10 populations of Ch. smymrnioides. Water-soluble components in the root of Zijinshan population was the most unique, similarity coefficients were relatively small among Zijinshan population and the other seven populations except Hongshan population, and similarity coefficient was in a higher level of 0.9697 between Zijinshan population and Hongshan population. Water-soluble components were extremely similar in four populations that were Laoshan, Maoshan, Qinglongshan and Langyashan, and similarity coefficients among them were in a high level exceeded 0.99. 10 populations were divided into 3 groups according to clustering results.

CONCLUSIONWater-soluble components show a high diversity in the roots of Ch. smyrnioides among different populations, and can be clearly divided into 3 types.

Apiaceae ; chemistry ; Chromatography, High Pressure Liquid ; Plant Extracts ; analysis ; isolation & purification ; Plant Roots ; chemistry

Objective:To optimize the diagnosis-related groups (DRG) weight calculation method based on the perspective of hospital performance evaluation and the high-quality development orientation of public hospitals.Methods:Using the first page data of 3 256 701 inpatient medical records from secondary and tertiary hospitals in Beijing from January to December 2021, three algorithms including payment DRG weight, five-category DRG weight, and optimized DRG weight were used to calculate the weights of each DRG, and the differences between different algorithms were analyzed. The case-mix index (CMI) of the entire hospital and the clinical specialties to which the key DRG belongs in secondary and tertiary hospitals was calculated by using the three DRG weight algorithms, to reflect the performance evaluation results of different algorithms.Results:The results of one-way ANOVA showed that there was a significant difference ( P=0.019) among the three DRG weight algorithms. Comparing the optimized DRG weight with the payment DRG weight, the weight of BD29 (neural stimulator implantation or removal surgery) in the key DRG decreased from 7.77 to 4.61, and the weight of LA19 (renal tumor surgery) increased from 2.06 to 2.58; Compared with the five-category DRG weight, the weight of ES31 (respiratory infection/inflammation with severe complications or comorbidities) decreased from 2.36 to 1.72, and the weight of CB39 (crystalloid surgery) increased from 0.22 to 0.30. Comparing the use of optimized DRG weights and five-category DRG weights to calculate CMI, all types of hospitals and clinical specialties showed varying degrees of improvement in CMI. The CMI of tertiary hospitals increased from 1.02 to 1.20, and the CMI of secondary hospitals increased from 0.88 to 0.95. The difference in CMI between secondary and tertiary hospitals was even more pronounced. Conclusions:Optimized DRG weights could better reflect the value of medical technology compared with the payment DRG weights. Compared to five-category DRG weights, optimized DRG weights could better reflect the differences in CMI of different levels of hospitals.