Activating transcription factor 3 (ATF3) is a protein produced by the cells in the ischemic reperfusion injury,neuronal or liver damage,skin damage,viral oncogene expression or DNA damage.Recent studies have found that ATF3 plays an important role in the occurrence and development of human malignant tumors.

Objective To improve the knowledge of mammographic signs of sclerosing adenosis.Methods The X-ray and pathological features of sclerosing adenosis of 17 focuses in 15 cases proved by histology were reviewed in comparison with that of breast cancer.The study was put emphasis upon the difference between sclerosing adenosis and breast cancer on clinic and radiographs.Results On mammography,the lesions appeared as asymmetric and disseminated density in 5/17(29.4%),dense nodules in 10/17(58.8%),calcifications inside the lesions in 3 and focal architectural disorder combined with stellate constriction in 2/17(11.8%).Conclusion The sclerosing adenosis is easily confused with breast carcinoma on radiographic signs,the diagnosis would be combined with clinical history and palpation examination.

Objective To evaluate the efficiency of injection of colloidal chromic phosphate 32 P cavity in rats with rheumatoid arthritis.Methods Collagen induced arthritis (CIA) was produced in Sprague Dawley rats.After one week immune,the right post ankle was given colloidal chromic phosphate 32 P intra articular injection.Severity of arthritis was observed by the left to right diameter of the right post ankle joint,the volume of right post paw and the arthritic index.Results The group treated with colloidal chromic phosphate 32 P intra articular injection developed milder arthritis compared with control CIA group after 6 weeks.The left to right diameter of the right post ankle joint was (3 96?0 05) mm of the colloidal chromic phosphate 32 P intra articular treated group compared with (4 11?0 08) mm of the control CIA group ( P

The calcium antagonist action of arctigenin (ACT) was studied in order to verify the ef-fect of Fructus Arctii for the relieve of exterior syndrome. Muscular contraction of isolated rat trachea,colon, pulmonary artery and thoracic aorta induced by KC1, that of guinea pig trachea induced by CaCI2,before and after the addition of ACT were assessed and their contraction-response curves drawn and PD'2calculated according to Scott. The inhibition rate of two-phase contraction of guinea pig trachea induced byacetylcholine chloride (Ach) in comparison with verapamil (VER) was calculated. Results of the studyshowed that ACT could non-compatitively antagonize the muscular contraction of the test specimens withPD'2 of 4.01, 5.11, 5.98 and 6.05 respectively. Similar to VER, ACT could non-competitively antagonizethe isolated guinea pig trachea with PD'2 of 4.04 and 5.62 respectively. Both of them could inhibit thefirst phase contraction induced by Ach with inhibition rates of 66.14% and 81.42% respectively. It wasconcluded that ACT, as the active constituent of Fructus Arctii, relaxed smooth muscle contraction byblocking the potential dependant Ca2+ channel and the internal release of Ca2+.

AIM:In order to ensure the quality stability of Fructus Polygoni Orientalis,to study the determination method of the fingerprint of Fruetus Polygoni Orientalis and to establish the fingerprint of Fructus Polygoni Orientalis.METHODS:The HPLC assay was used to establish the fingerprint of Fructus Polygoni Orienlalis and 28 pieces of goods were compared.RESULTS:The fingerprint of Fructus Polygoni Orientalis with 7 common peaks was established.The relative retention time and the ranges of relative area of the common peaks were determined.CONCLUSION:The established fingerprint can be used for the quality control of Fructus Polygoni Orientalis.

Objective Toinvestigatethevalueofimagingdiagnosisofthe lymphangioleiomyomatosis( LAM ).MethodsFifteen patients with LAM confirmed by pathological assessment were analyzed retrospectively for radiologic findings.They had chest radiograph, chest highresolution CT (HRCT),abdominal CT, direct lymphangiography(DLG), chest CT and abdominal CT after DLG.Results Chest radiograph findings included normal (1),increasing of lung markings (3),disseminated honeycomb or reticular pattern ( 11 ), pneumothorax ( 2 ), and pleural effusion ( 14 ). Chest conventional CT and HRCT showed typical imaging manifestation of PLAM in all cases, including sporadic or disseminated cysts in bilateral lungs. According to the grading standard of pulmonary disease made by Avila et at, there were 3 cases in grade Ⅰ , 5 cases in grade Ⅱ and 7 cases in grade Ⅲ . Fourteen of 15 patients with LAM had positive abdominal CT findings in retroperitoneum and pelvic cavity. Common abdominal CT findings included cystic lymphangioma in 9 of 14 patients, lymphangiomyoma in 13 and both coexisting in 7.One of the14patients alsohadhepaticlipomaandangiomyolipomas.Onepatienthadrenal angiomyolipomas; and one patient had hysteromyoma. All 15 cases underwent DLG, 1 cases had lymphatic obstruction in the lumbar 3 level, the remaining 14 cases had varying degrees of thoracic duct stenosis, or obstruction. Neck trunk, subclavian trunk and bronchial trunk showed lymphatic reflux. On post-DLG CT,thoracic duet outlet obstruction was not demonstrated in 3 cases, the remaining 12 cases showed thoracic outlet obstruction, consistent with the DLG findings.Conclusion HRCT is a useful diagnostic method showing characteristic findings of PLAM. MSCT can help to detect abdominal LAM. DLG and MSCT after DLG have value in displaying obstruction site of thoracic duct or lymphatic trunks and provide guidance for operative treatment.

Objective To assess the diagnostic value of confoeal laser endomicroscopy(CLE) in histological gastritis.Methods A total of 149 patients underwent CLE.Standard locations and focal lesions were examined by confocal imaging, and specimens were taken by targeted biopsy.The relationship between confocal images and histological findings were studied systemically.Results The sensitivity,specificity and accuracy of CLE in diagnosis of atrophy were 83.6%,99.6%and 97.5%,respectively;and those in moderate to severe neutrophil infiltration were 81.9%,99.3%,and 95.8%,respectively.The mean kappa values for inter-and intra-observer agreement on the classification of confocal images in groups were 0.749 and 0.811 respectively.Conclusion The validity and reproducibility of CLE in diagnosis of histologic gastritis are promising,which might warrant its further application in clinical practice.

OBJECTIVETo study the antitumor activities of the liposoluble components from caulis Marsdenia tenocissimae and analyze its chemical constituents.

METHODThe supercritical fluid extraction was used to extract the liposoluble components from caulis M. tenocissimae. MIT test was applied to study the anticancer effects of liposoluble components on growth inhibition in human liver cancer line HepG-2 cells, and the chemical constituents were analyzed by gas chromatography/mass spectrometry (GC-MS).

RESULTThe liposoluble components of caulis M. tenocissimae have obvious growth inhibitory action to HepG-2 cells. Twenty-four compounds were identified and their relative contents were determined.

CONCLUSIONThe liposoluble components from caulis M. tenocissimae have antitumor activities, and its pharmacological activity is related to its chemical constituents.

Antineoplastic Agents ; analysis ; chemistry ; pharmacology ; Cell Proliferation ; drug effects ; Gas Chromatography-Mass Spectrometry ; Hep G2 Cells ; Humans ; Lipids ; chemistry ; Marsdenia ; chemistry ; Solubility

Objective To investigate the clinical value of CT lymphangiography (CTL) combined with direct lymphangiography (DLG) in primary intestinal lymphangiectasia (PIL). Methods Sixteen patients diagnosed as PIL with intestinal enteroscopy were recruited in this retrospective study. All the patients were performed DLG and CTL one week before exploratory laparotomy. Subjective assessment in DLG included weak lymphatic fluid drainage, lymphangiectasia, lymphatic reflux, fistula and thoracic outlet reflux or obstruction. While for CTL combined with DLG, the intestinal and extra-intestinal lesions were evaluated, including lymph node, edema, lymphangiectasia and abnormal distribution, fistula, and lymphangiomatosis. All the diagnosis was compared with intestinal endoscopy results. Results For DLG, 16 weak lymphatic fluid drainages, 9 lymphangiectasia, 1 fistula with abdomen and 14 thoracic outlets weak lymphatic fluid drainage or obstruction were found. For DLG combined with CTL, 16 intestinal lumens dilatation and 14 circumferential intestinal thickening were found in intestinal lesions. While for extra-intestinal lesions, the imaging features included edematous findings (12 in mesentery, 7 ascites only, 2 hydrothorax and ascites, and 3 pericardial, thoracic and abdominal effusions), abdominal lymph nodes (6 cases), lymphangiectasia and abnormal distributions (14 cases), fistulas (lymph-intestinal luminal fistula in 4 cases, and lymph-abdominal fistula in 3 cases), lymphangiomatosis (3 cases), and thoracic duct outlet dysfunction and reflux (14 cases).The number of cases diagnosed as intestinal lymphangiectasia, intestinal luminal lymph exudation and lymph fistula were 16, 10 and 6 with intestinal endoscopy, while the number were 11, 0, and 4 with CTL combination with DLG. Conclusion Combination of CTL with DLG is valuable in the diagnosis of PIL.

Objective To evaluate the application of pathological diagnosis by rapid paraffin sections in the diagnosis and treatment of cervical diseases. Methods A total of 176 cases from our hospital between September 2009 and January 2010 with abnormal cervical cancer screening (including abnormal cytology result and high-risk HPV continuous positive) were randomly divided into 2 groups. Eighty-seven cases of them whose biopsy were got by Belinson forceps under the direction of colposcopy with rapid paraffin sections by ultrasonic histopathological rapid processor and BT transparent agents were selected as group A, while 89 cases with conventional paraffin sections were selected as group B. The production time and quality for paraffin sections were analyzed in the two groups. Those diagnosed as cervical intraepithelial neoplasia (CIN)Ⅱ or even worse and some special patients with CIN Ⅰ in the two groups received surgery, including loop electrosurgical procedure (LEEP) ,cold knife conization (CKC),.hysterectomy or radical hysterectomy.Tissue obtained after surgery was sent for routine pathological examination. If the results of postoperative routine pathological examination were inconsistent with the rapid or routine biopsy pathological examination,the heavier results were regard as the final diagnoses. The pathological results and diagnose accordance rates were recorded and compared between group A and group B. Results The quality of sections in two groups were all satisfied or basically satisfied to meet the diagnostic requirements. There were statistically significant difference in average production time between group A and B (40 minutes vs 24 hours, P < 0. 05). Thirty patients in group A and 32 patients in group B received surgery. The coincidence rate of biopsy pathological results and final diagnoses were 93% (28/30) for group A and 91% (29/32) for group B, in which there were not statistically significant difference (P > 0. 05). Conclusion Bapid paraffin sections technology is safe, accurate and economical for rapid pathological diagnosis of cervical diseases, which is worthy for being widely used in hospitals.