Objective To analyze the complications of pregnant secondary cesarean in scar uterus,to provide theoretical guidance for cesarean section.Methods 160 pregnant women of secondary cesarean uterine scar were chosen as the study group.80 pregnant women of non-scar uterine were chosen as the control group.Results The bleeding volume during operation,operation time,operation fee,postoperative bleeding,uterine resection rate,neonatal asphyxia in study group were (432 ± 331) mL,(64 ± 21) min,(0.91 ± 0.11) million,(262 ± 187) mL,4.4％,11.9％,which in the control group were (361 ± 209) mL,(44 ± 16) min,(0.63 ± 0) million,(184 ± 132) mL,1.3％,6.3％ respectively,the differences were significant (t =2.52,8.19,26.10,4,4.76,x2 =3.98,all P ＜ 0.05).The two groups had no statistically significant difference in organ damage situation (P ＞ 0.05).Conclusion Secondary cesarean uterine scar easily lead to maternal blood loss and increased postoperative bleeding,prolonged surgery,increased hysterectomy rate,increased incidence of neonatal asphyxia and other complications.Pregnant uterine scar pregnancy is a high-risk pregnancy,pregnancy for maternal uterine scar secondary surgical indications should be strictly controlled.

Objective To investigate the effects ofviaminate on the proliferation and differentiation of HaCaT cells,a human keratinocyte cell line.Methods Cultured HaCaT cells were treated with various concentrations (2,5,10,15,20,25 and 30?g/mL) of viaminate for various durations.The cell proliferation was assessed by MTT method,the changes of cell cycle and apoptosis rate by flow cytometry,the changes of keratin 10 and involucrin mRNA expressions by semi-quantitative reverse transcription PCR.Results The proliferation of HaCaT cells was inhibited by the treatment with viaminate of≥2?g/mL for 48 h,and the inhibition rate was raised with the increase of treatment time and dosage.The viaminate of 30?g/mL inhibited the proliferation of HaCaT cells by 57.67% and 82.00% at 48 and 72 h after the incubation respectively,and elevated the mRNA expression of involucrin from 40.80% to 156.12%,decreased the mRNA expression of keratin 10 from 96.46% to 14.60%.The mRNA expression of involucrin increased with the elevation of viarninate dosage.Under the treatment with viaminate for 48 h,the cell population at G_1 phase significantly increased,that at S and G_2 phases decreased;the switching of G_1 to G_2 was inhibited;but the cell apoptosis was not affected.Conclusion Viaminate could inhibit the proliferation and induce the differentiation of keratinocytes.

Objective:To establish an HPLC method for the determination of EDTA-2Na in amphotericin B. Methods: A Waters C18 column(50 mm × 4. 6mm, 5 μm) was used. The mobile phase A was acetic acid solution (1. 5 ml acetic acid was added into 1000ml water, and 41 ml 10% tetrabutylammonium hydroxide solution was added), and the mobile B was acetonitrile with gradient e-lution. The flow rate was 0. 8 ml·min-1 , the column temperature was 30℃, the detection wavelength was 260 nm and the injection volume was 25μl. Results:The results showed that EDTA-2Na in amphotericin B could be detected without any interference. The cal-ibration curve of EDTA-2Na was linear within the range of 0. 92-7. 37μg·ml-1(r=0. 999 9), the LOD was 1. 93 ng·ml-1 and the LOQ was 6. 45 ng·ml-1. The average recovery was 102. 5% (RSD=2. 8%, n=9). Conclusion: The method is simple, selective and accurate. It can be used for the quality control of EDTA-2Na in amphotericin B.

Objective To establish a method for the determination of methyl tertiary butyl ether (MTBE) in the cerebrospinal fluid of rats by gas chromatography and to know whether MTBE can penetrate the blood brain barrier. Methods 9 male SD rats were randomly divided into 3 groups and then exposed to MTBE by gavage at a dose of 1000 mg/kg?bw and by inhalation at a dose of 8 ml/L for 30 days. The cerebrospinal fluid was collected at 6 h, 12 h and 24 h respectively at the end of treatment and the MTBE in rats cerebrospinal fluid was determined by gas chromatography. Results MTBE was detected in the cerebrospinal fluid of rats after subchronic exposure of MTBE through gavage and inhalation. Conclusion MTBE is able to penetrate the blood brain barrier and reach the brain.

Objective:To study the asaphia in children with ankyloglossia and the effect of articulation therapy after anaplasty.Methods:Articulation analysis by testing 21 shengmu and 8 yunmu, particularly the articulation involving front tongue was carried out in 16 healthy children and in 32 children with ankyloglossia before and after treatment.Among the 32 children with ankyloglossia 16 were treated with articulation training 1 month after operation(trained group),another 16 had the articutation test 2 months after operation(untrained group).Results:The articulation clarity in the children with ankyloglossia and in the healthly controls was 29.36 and 97.86 respectively(P

Objective To introduce the current studies of the role of vascular endothelial growth factor-C(VEGF-C) and VEGF-D in lymphangiogenesis and lymph node metastasis of gastrointestinal neoplasma.Methods The related literatures in recent 5 years were reviewed.Results The growth factors VEGF-C and VEGF-D enhance lymphangiogenic metastasis of gastrointestinal neoplasma with the property of angiogenesis and lymphangiogenesis.In gastric adenocarcinoma,VEGF-C mRNA and tissue protein expression correlate with lymphatic invasion,lymph node metastasis,venous invasion and reduced 5-year survival rates.The role of VEGF-C in esophageal squamous cancer and colorectal cancer and VEGF-D in colorectal cancer is not certain,with conflicting reports in the published literatures.Conclusion The VEGF-C,VEGF-D/VEGFR-3 signal pathway may become the ideal target for inhibition of tumor proliferation and metastases,antilymphangiogenesis therapy may be a novel potential strategy in tumor biological therapy.

BACKGROUND: Electroacupuncture has a good clinical effect on Alzheimer disease, but its mechanism remains unclear.OBJECTIVE: To observe the effect of electroacupuncture on number of synaptic numeric density (Nv), surface density (Sv) and average size of synaptic conjunction and ultrastructure in hippocampal CA3 area of neurons of model rats with Alzheimer disease (AD).DESIGN: Completely randomized grouping and controlled study.SETTING: Department of Traditional Chinese Medicine of Sichuan Provincial People's Hospital; College of Acupuncture & Moxibustion and Massage of Chengdu University of Traditional Chinese Medicine.MATERIALS: A total of 50 male SD rats of 24-month old weighing (480±20) g and 6 male rats of 3-month old weighing (250±15) g were selected in this study. Passage water maze (2.1×1.7×0.6) m3 was made of black glasses with 40 cm deep water and 4 caecums. WQ1002F Hans electrically heated distilling apparatus was used.METHODS: The experiment was carried out at the Grade Ⅲ Animal Experimental Center of Chengdu University of Traditional Chinese Medicine between, September 2002 and June 2003. ① Old SD rats were grouped on the basis of water-maze test results. Firstly, 6 young rats were submitted to water-maze test at 8 days before modeling for 4 continuous days to obtain average escaping latency. Secondly, 50 old rats were accepted in the water-maze test at 4 continuous days before modeling to obtain average escaping latency. A total of 36 rats whose latencies were less than average values plus one standard deviation of young rats were regarded as normal old rats. Among them, 12 rats were randomly divided into control group and sham operation group with 6 in each. Another 24 rats were transected at fornix-fimbria AD modeling. Thirdly, 24 modeling rats were all adopted in the water-maze test at 2 days after modeling. Rats whose latencies were more than average value plus two standard deviation of young rats were chosen as AD model. Twelve AD models were randomly divided into model group and electroacupuncture group with 6 in each.② On the 6th day after modeling, rats in electroacupuncture group were acupunctured at Baihui (Du20) of 0.5 inch in slope, Yongquan (K1),Taixi (K3) and Xuehai(Sp10) of 0.3 inch in depth with No. 30 milli-needle (3.33 cm); Then, electrically heated distilling apparatus was used with successive waves, 20 Hz in frequency and 2-4 V in voltage. Tolerant stress of rats at quiet state was regarded as the standard value (2 mA),the needle was maintained for 30 minutes, and the acupuncture was done once a day for totally 20 successive days. Rats in control group sham operation group (cerebral cortex was exposured at the same site as model rats, and fornix-fimbria was not cut off) and model group were only fixed but treated with nothing. ③ After treatment, ultrastructure in hippocampal CA3 area of rats was observed with transmission electron microscope;synapse numbers and cross-point numbers between synaptic conjunction and test line were counted with stereological technique; stereological parameters, such as numeric density (Nv), area surface (Sv) and average size of synaptic conjunction, which could reflect plastic changes of synaptic form, were calculated at the same time. ④ Differences between every two groups were compared with t test at regular variance and t'test at irregular variance.MAIN OUTCOME MEASURES: Comparisons of numeric density (Nv),surface density (Sv) and average size of synaptic conjunction.RESULTS: A total of 24 old rats were involved in the final analysis with 6 in each group. ① Ultrastructure: Synaptic density of control and sham operation group was higher than that of model group, and average area of synapse was smaller;, synaptic density of electroacupuncture group increased compared with that of model group, and average area of synapse was smaller. ② Numeric density and surface density in hippocampal CA3 area in model and electroacupuncture group were lower than those of control group and sham operation group (P ＜ 0.05). There was no significant difference between sham operation group and control group (P ＞ 0.05), and those of electroacupuncture group were higher than those of model group (P ＜ 0.01). ③ Average size of synaptic conjunction in hippocampal CA3 of model and electroacupuncture group was higher than that of control and sham operation group (P ＜ 0.05). There was no significant difference between sham operation group and control group (P ＞ 0.05), and the value of electroacupuncture group was lower than that of model group (P ＜ 0.01). CONCLUSION: Electrotherapy can repair synaptic form and inhibit synaptic degeneration of hippocampal neurons in AD rats.

Objective To study the analysis of ritodrine hydrochloride in comparison with Magnesium Sulfate expect application effect in the treatment of placenta previa.Methods In Wenling Maternal and Child Health Care Department from January 2014 to December 2016 were 100 cases of expectant treatment of placenta previa patients as the research object in the course of the study, were randomly divided into control group and experimental group two were 50 cases each.The control group was treated with the Magnesium Sulfate treatment, patients in the experimental group of ritodrine hydrochloride.A comparative analysis of the experimental group and the control group of patients with successful pregnancy, prolonged pregnancy and neonatal weight and other indicators, including the adverse reactions of patients.Results After the treatment,the number of success cases in experimental group was 48 cases, the success rate was 96%,the number of cases to 42 cases stopped bleeding during pregnancy, to extend the time for(16.2±12.0)days, neonatal weight(3.21±0.35)kg.The number of cases of successful pregnancy control group of patients was 41 cases, the success rate was 82%,the number of cases to 36 cases stopped bleeding during pregnancy, to extend the time for(12.2±10.2)days, neonatal weight(2.39±0.48)kg.Available, the experimental group success time extension of pregnancy and neonatal weight were significantly better than the control group, with statistical difference(P<0.05).Can be obtained, the experimental group and the control group, the incidence of adverse reactions were statistically significant.Conclusion Ritodrine hydrochloride and Magnesium Sulfate expect application effect in the treatment of placenta previa compared the clinical effect of ritodrine hydrochloride, can effectively improve the indicators for further promotion and application, has the clinical significance.

Objective To construct a recombinant Bifidobacterium bifidum (Bb)vaccine[Bb (pGEX-Sj26GST-Sj14-3-3)] of Schistosomajaponicum (Sj) and analyze the expression of the fusion gene Sj26GST-Sj14-3-3 of Sj in Bb.Methods The recombinant plasmid pGEX-Sj26GST-Sj14-3-3 was electroporated into Bb to construct a recombinant Bb (pGEX-Sj26GST-Sj14-3-3) vaccine.Mter induction with isopropyl-β-D-thiogalactoside (IPTG),double restriction enzymes digestion and polymerase chain reaction (PCR) were used to identify the recombinant Bb (pGEX-Sj26GST-Sj14-3-3),expression of the recombinant protein was analyzed and identified by sodium dodecyl sulfonate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting.Results The recombinant plasmid pGEX-Sj26GST-Sj14-3-3 was successfully transformed into Bb identified by double restriction enzymes digestion and PCR.SDS-PAGE analysis showed that the relative molecular mass of the expressed recombinant protein was approximately 67 × 103.The expressed protein could be recognized by the immune sera from rabbits infected with Sj by Western blotting.Conclusions The recombinant Bb (pGEX-Sj26GST-Sj14-3-3) vaccine of Sj is successfully constructed.The fusion gene Sj26GST-Sj14-3-3 can be expressed in recombinant Bb and the expressed target protein shows specific antigenicity.

Objective To investigate the effects of sodium arsenite (NaAsO2) on cell survival circumstance,reactive oxygen species (ROS) and cell apoptosis in human normal hepatic cells (L-02).Methods L-02 cells were exposed to different doses of NaAsO2 (0,50,100,150 μmol/L) for 24 h.MTT assay was used to detect the survival of L-02 cells,and flow cytometry (FCM) was used to detect the ROS levels and the early (Q4),late (Q2) apoptosis of L-02 cells.Results Cell survival rate:cell survival rate was compared between groups,the difference was statistically significant (F =350.51,P ＜ 0.05),the cell survival rates of 50,100 and 150 μmol/L NaAsO2 groups [(87.30 ± 3.74)％,(49.03 ± 4.72)％,(13.44 ± 4.01)％] were significantly lower than that of the control group [(100.00 ± 0.00)％,all P ＜ 0.05];compared with 50 μmol/L NaAsO2 group,the cell survival rates of 100 and 150 μmol/L NaAsO2 groups were significantly decreased (all P ＜ 0.05);compared with 100 μmol/L NaAsO2 group,the cell survival rate of 150 μmol/L NaAsO2 group was significantly decreased (P ＜ 0.05).The ROS levels:ROS levels were compared between groups,the difference was statistically significant (F =407.78,P ＜ 0.05),the ROS levels of 100 and 150 μ mol/L NaAsO2 groups (3 212.00 ± 221.93,5 521.33 ± 179.63) were significantly higher than that of the control group (1 691.67 ± 73.98,all P＜ 0.05);compared with 50 μmol/L NaAsO2 group (1 927.67 ± 62.45),the ROS levels of 100 and 150 μmol/L NaAsO2 groups were significantly increased (all P ＜ 0.05);compared with 100 μmol/L NaAsO2 group,the ROS level of 150 μ mol/L NaAsO2 group was significantly increased (P ＜ 0.05).Cell apoptosis:cell apoptosis rates of Q2,Q4 and Q2 + Q4 were compared between groups,the differences were statistically significant (F =256.84,26.53,63.89,all P ＜ 0.05);excecpt the cell apoptosis rate of Q4 in 50 μ mol/L NaAsO2 group [(5.43 ± 0.57) ％],the cell apoptosis rates of Q2 [(5.67 ± 0.21)％] and Q2 + Q4 [(11.10 ± 0.40) ％] in 50 μ mol/L NaAsO2 group,the cell apoptosis rates of Q2 [(13.60 ± 0.79) ％],Q4 [(7.37 ± 2.01) ％] and Q2 + Q4 [(20.97 ± 2.38) ％] in 100 μmol/L NaAsO2 group,the cell apoptosis rate of Q2 [(13.47 ± 0.78) ％],Q4 [(16.97 ± 3.45) ％] and Q2 + Q4 [(30.43 ± 3.84) ％] in 150 μmol/L NaAsO2 group were significantly higher than those of the control group [Q2:(3.47 ± 0.12) ％,Q4:(2.90 ± 0.90) ％,Q2 + Q4:(6.37 ± 1.00) ％,all P ＜ 0.05];compared with 50 μmol/L NaAsO2 group,the cell apoptosis rates of Q2,Q4 and Q2 + Q4 in 100 and 150 μmol/L NaAsO2 groups were increased,except the cell apoptosis rate of Q4 in 100 μ mol/L NaAsO2 group,the differences were statistically significant (all P＜0.05);the cell apoptosis rates of Q4 and Q2 + Q4 in 150 μmol/L NaAsO2 group compared with 100 μmol/L NaAsO2 group were significantly increased (all P ＜ 0.05).Conclusions NaAsO2 can induce L-02 cells to increase ROS levels,and inhibit L-02 cell proliferation.In addition,NaAsO2 can induce early apoptosis and late apoptosis in L-02 cells.