Objective To explore the factors related with osteoporosis in middle-aged men who accepted high education. Methods 306 men were investigated about their bone density, also their behaviors, diet, exercise and so on. Results The incidence of osteoporosis increased with the aging. The Logistic regression showed that smoking (OR=4.61) and alcohol intake (OR=1.11) were the risk factors for osteoporosis,while the milk intake (OR=0.19) and exercise (OR=0.13) were the protective factors. Conclusion The low bone mass and osteoporosis of middle-aged men accepted high education are associated with the age, smoking, alcohol or milk intake and exercise.

Objective :To Study the Relationship between the Qi Deficiency and Blood Stasis Syndrome and artificial hematogenous metastasis of colon cancer by establishing a compound model of Qi Deficiency and Blood Stasis Syndrome and artificial hematogenous metastasis of colon cancer. Methods:Thirty six eight-weeks old female Balb/c rats were divided into six groups randomly:control group ,stasis group,tumor groupA、B ,stasis and tumor groupA、B. We observed the survival time of tumor groupB and stasis and tumor groupB. At the 28th day,after killing the mice of the other groups,we detected the whole blood viscosity and the amounts of metastasis tubercle on lung. Results:The weight of the stasis group increase more slowly than control group(P

bjective To induce a treatment program of evidence-based acupuncture and moxibustion for oculomotor paralysis by retrieving and analyzing the current literature for improving clinic technology.MethodsOne case was used as example and it was introduced how to raise clinical questions,retrieve relative literature,evaluate the retrieval literature and at last,stipulate treatment program based on the retrieval evidences.ResultsThe acupuncture and moxibustion treatment program for oculomotor paralysis established by evidence-based medicine was adopted to treat the patient for 10 times,as a result the patient got much better.Conclusion: Through clinically raising questions,searching for evidence,analyzing evidence,making a strategy decision,practicing evidence-based course,the patient could attain satisfactory therapeutic effect,and the physician could raise theoretical level and clinical ability.

Objective To investigate pathogenesis of liprd metabolism disorder in children with nephrotic syndrome. Methods Serum lipid and plasma llpoprotein lipase and hepatic lipase were detected in 62 nephrotic syndrome children and 30 normal children, respectively. Results The activity of lipoprotein lipase and hepatic lipase was lower than that in normal control group, while serum cholesterol, triglycerides and low -density lipoprotein in nephrotic group were higher than those in control group. Lipoprotein lipase and hepatic lipase were negative correlation with triglycerides and low - density lipoprotein, respectively. Conclusions Reduced activity of lipoprotein lipase and hepatic lipase is one of causes leading to hypertriglyceridemia in nephrotic syndrome.

Objective To explore the relationship between poor sleep quality and stoke.Methods A total of 738 stroke patients in Xuzhou city in 2013 were selected as the case group and age-and sex-matched healthy non-stroke subjects (n =738)as control group.The writer-designed general situation questionnaire and the Pittsburgh Sleep Quality Index(PSQI)analyses were conducted for a face-to-face investigation.Results No significant difference in mean age(66.1±10.9 and 65.8 ± 10.6,t =0.60,P =0.58)and in sex (50％ vs.50 ％) was found between two groups.There were statistically significant differences between case and control groups in baseline values of BMI(t=2.40,P =0.02),histories of hypertension(x2 =174.30,P =0.00),diabetes mellitus (x2 =27.20,P =0.00),coronary heart disease(x2 =115.60,P =0.00),smoking(x2 =6.10,P =0.01),drinking (x2 =7.30,P =0.01)and living stress(x2 =11.40,P =0.01).The PSQI sub-scores and PSQI total scores were higher in case group than in control group.The rate of poor sleep quality was higher in case group(279 cases,37.8 ％) than in control group(136 cases,18.4 ％) (x2 =6.10,P =0.01).Multivariate logistic analysis showed that,after adjusting for confounding factors of BMI,histories of hypertension,diabetes,coronary heart disease.smoking,drinking and living stress,the poor sleep quality in total male plus female was independent predictor variables for stroke[odds ratio(95 ％ CI) of 2.3 (1.8-3.0)],no matter their sex,with odds ratio (95 ％ CI) in male (2.5,1.7-3.7) or in female (2.2,1.5-3.2),respectively,but there was no significance difference in the odds ratio between male and female in case group(x2 =0.04,P=0.85).The risk stroke was 2.3 folds higher in poor sleep quality versus control in male plus female,with pure male or female of 2.5 or 2.2 folds.There was no significance difference between male and female in case group versus.the control(x2 =0.04,P=0.85).Conclusions Poor sleep quality is associated with the occurrence of stroke and may be a risk factor for stroke.

Objective To express human chemokine-like factor 1 (CKLF1) in Drosophila S2 cells and study its function. Methods The pMT/V5-His-CKLF1 expression plasmid was constructed and transfected into Drosophila S2 cells. The positive clones were selected through PCR and RT-PCR. The culture medium was analyzed by Western blot with anti-CKLF1 polyclonal antibody. Chemotaxis and MTT assays on human peripheral blood and C2C12 cells, respectively, were then carried out with the medium. Results CKLF1 was transcribed efficiently in S2 cells. The expressed CKLF1 protein could be detected in the culture supernatant by Western blot, which showed weak chemotactic activity on both human peripheral blood neutrophils and lymphocytes as well as enhancing effect on the proliferation of C2C12 cells. Conclusion CKLF1 was expressed successfully in Drosophila S2 cells and secreted into the culture medium. The recombinant CKLF1 expressed in Drosophila cells can chemoattract leucocytes and promote the proliferation of C2C12 cells.

Objective:To discuss the results interpretation and clinical significance of Acinetobacter Baumanni ( AB) positive spu-tum samples .Methods:The anti-infection treatment of one patient with lung infection after colon cancer surgery in ICU was analyzed , and the results interpretation and clinical significance of AB positive sputum samples were discussed .Results:Although the culture re-sults of sputum samples were positive , the quality of sputum samples was low and the credibility was poor .The possibility of multiple drug resistance AB ( XDR-AB) screened by antibiotics selective stress was small .Meanwhile, the clinical infection symptoms were mild, and the treatment with imipenem was effective .Although the patient had high risk factors for the colonization of Baumanni infec-tion, XDR-AB was not a pathogen .Conclusion:When respiratory samples are AB positive , the quality of samples should be evaluated by smear results firstly , especially the existence of white blood cell phagocytosis or accompanying should be paid attention to , and then the possibility of AB screened by antibiotics selective stress and high risk factors for colonization should be analyzed .Finally, combined with the clinical symptoms of patients and the treatment efficacy before drug sensitivity tests , whether XDR-AB is pathogenic bacteria should be judged , and then the corresponding anti-infection treatment plan should be determined .

Objective To design a post-peripheral intervention observation form that uses the timepoint and observation project as the framework of contents,and to evaluated its clinical application effect.Methods Non-concurrent patients were used to make a control study.By using the convenient sampling method,101 patients,who received peripheral interventional therapy during the period from June 1,2014 to December 31,2014,were collected and used as the control group;routine nursing care was adopted and the results were documented.A total of 102 patients,who received peripheral interventional therapy during the period from January 1,2015 to June 30,2015,were used as the intervention group,for whom routine nursing care was carried out and self-designed post-peripheral intervention observation form was employed to record the nursing observation.The results of the two groups were statistically analyzed.After using the form,every month 10 patients,5 visiting physicians and 5 responsible nurses were asked to make an evaluation of nursing quality satisfaction.Results The incidence of main postoperative complications in the intervention group was lower than that in the control group,the chi-square test showed that the difference between the two groups was statistically significant (P＜0.05).The average hospitalization days and the mean medical expense of the intervention group were lower than those of the control group,the chi-square test indicated that the difference between the two groups was statistically significant (P＜0.05).After using this form,the nursing quality satisfaction of patients and visiting physicians was improved,the self-rating satisfaction assessed by responsible nurses was also improved,the chi-square test revealed that the difference between the two groups was statistically significant (P＜0.05).Conclusion All the evaluation indexes of the patients in the intervention group are better than those of the patients in the control group,indicating that this observation form is reasonably designed,meets the specialty characteristics,heightening the dynamic observation for patients after peripheral intervention,strengthens the effectiveness of monitoring postoperative complications,and is helpful for the implementation of the observation after peripheral intervention.Therefore,this observation form is worth promoting use.

Objective To analyze the factors which influence the present nursing management ability in the alliance and to provide theoretical evidences and methods to improve the management. Methods 120 nursing administrators from 7 hospitals in the alliance were selected in convenience sampling for the site survey with customized questionnaires on nursing management ability appraisal. Results The valid return rate of the questionnaires is 100%;the average score of all respondents is 82.84±10.10;the differences in nursing management between the leading hospital and its members are statistically significant (P <0.05)while the differences among the members are not.Conclusion The leading hospitals play a very important role in improving the nursing management ability and administration in the alliance,which should integrate and leverage leadership roles to develop high quality nursing management among the hospitals in the alliance.

Objective To analyze the content and methods of different humanistic nursing care evaluation tools,both domestic and abroad,and to provide reference for the development of more objective,scientific and operational evaluation tools.Methods The CNKI,Wanfang database,CqVIP,PubMed and Cochrane library database were retrieved by computer,and supplemented by manual searches.Then the articles which in accordance with inclusion criteria were analyzed by descriptive analysis.Results A total of 25 literatures were involved,including 22 foreign literatures,while only 3 in domestic.Conclusions The foreign humanistic nursing care evaluation tools are more mature at present,but China is still in its infancy,so it should develop humanistic nursing care evaluation tool which conforms to Chinese culture and national conditions,objective and scientific,in order to enhance humanistic caring capacity of nurses in China.