Objective To investigate the safety and feasibility of laparoscopic surgery in the treatment of common bile duct stones after subtotal gastrectomy.Methods A retrospective analysis was performed for the clinical data of 46 patients with gallstones and common bile duct stones after subtotal gastrectomy who underwent surgical treatment in Luoyang Central Hospital Affiliated to Zhengzhou University from January 2010 to October 2016.Among these patients,25 underwent laparoscopic cholecystectomy + common bile duct exploration (laparoscopic group),and 21 underwent open cholecystectomy + common bile duct exploration (open group).The surgical conditions and postoperative complications were compared between the two groups.The t-test was used for comparison of continuous data between groups,and the chi-square test was used for comparison of categorical data between groups.Results No patients died during the perioperative period and 2 patients (8.0％) in the laparoscopic group were converted to open surgery.There were significant differences between the laparoscopic group and the open group in time to ambulation after surgery (1.2 ± 0.6 d vs 2.4 ± 1.2 d,t =4.395,P ＜ 0.001),time to passage of gas by anus after surgery (1.8 ±0.5 d vs 2.8 ±0.8 d,t =5.168,P ＜0.001),and length of postoperative hospital stay (5.2 ± 1.1 d vs 7.5 ±2.3 d,t =4.439,P ＜ 0.001).There were no significant differences between the two groups in time of operation,intraoperative blood loss,hospital costs,T tube placement,and rate of residual stones (all P ＞ 0.05).After surgery,2 patients (8.0％) in the laparoscopic group and 3 (14.3％) in the open group experienced complications,and there was no significant difference between the two groups (P =0.495).Conclusion Laparoscopic surgery is safe and feasible in the treatment of common bile duct stones after subtotal gastrectomy and has the advantages of minimally invasive surgery.

Constriction, Pathologic ; diagnosis ; pathology ; therapy ; Humans ; Palate, Soft ; pathology ; Sleep Apnea, Obstructive ; diagnosis ; pathology ; therapy ; Tongue ; pathology ; Uvula ; pathology

Child, Preschool ; Foreign Bodies ; surgery ; Humans ; Intestines ; Laparoscopy ; methods

Epilepsy ; diagnosis ; drug therapy ; surgery ; Humans ; Magnetoencephalography ; Positron-Emission Tomography ; Stereotaxic Techniques ; Surgery, Computer-Assisted ; methods ; Treatment Failure

BackgroundAdenoid cystic carcinoma is a malignant tumor originating from lacrimal gland epithelium with high recurrence rate and mortality because of its invasiveness. Although surgery, radiotherapy and chemotherapy are used clinically,the curative effect is not enough satisfied. ObjectiveThis study was to provide an experimental basis for clinical application of 125 Ⅰ seeds interstitial brachytherapy. MethodsHuman ACC-2 cell links were transplanted subcutaneously in the back of 30 SPF BALB/C nude mice to establish the ACC model,and 25 of these mice with suitable sizes of tumor were selected and randomly divided into the G1 (0. 4 mCi 125 Ⅰ ), G2 (0. 6 mCi 125 Ⅰ ) ,G3(0. 8mCi 125 Ⅰ ) ,and G4( 1.0mCi 125 Ⅰ ) groups according to the different therapeutic radioactivity treatments,with 5 nude mice for each group,and a 125 Ⅰ seed without radioactivity was used in 5 mice as the control group. The dimensions of the tumors were measured at 2-day intervals and the inhibition rates of tumors were calculated. Nude mice were killed 14 days later by the broken neck method,and the amount of apoptosis and necrosis as well as the maximum effective radius of tumor to 125Ⅰseed were detected under the transmission electron microscope and routine pathological examination. ResultsFourteen day after operation, the dimension of tumors was (3713.19±243.23)mm3 in the G0 group;while in the G1 ,G2 ,G3 and G4 groups,the dimensions of tumors were (3113.35±316.54) mm3,(2635.85±261.21) mm3, (2538.37±312.16) mm3 and(1686.28±231.65) mm3,respectively, showing a significant decrease in comparison with the Go group( P＜0. 05 ). The tumor inhibitory rates in the G1 ,G2,G3 and G4 group were(20. 11±3.09)％, (36. 18±2.54)％ ,(40. 83±4. 17)％ ,and(66. 63±5.34)％ ,with an obvious elevation with the increase in the dose of 125 Ⅰ ( F=120. 240,P=0. 000). Correlative analysis showed that the intensity of radioactivity from 125Ⅰhad a positive correlation with tumor inhibitory rate (r =0. 653,P =0. 008 ). The maximum effective radius were ( 5.2 ±0.5 ) mm, ( 6.4 ±0. 7 ) mm, ( 7.4 ±0.4 ) mm, and ( 8.2 ±0. 5 ) mm in the G1 ,G2, G3 and G4 groups, with the considerable differences among them (F=29. 22, P=0. 000). Radioactivity of 125 Ⅰ exhibited positive correlation with the maximum effective radius ( r =0. 609, P =0. 004). Conclusions125 Ⅰseed implantation brachytherapy can inhibit the growth of the transplanted ACC in BALB/C nude mice by suppressing the proliferation of tumor cells. It is a safe ,feasible and effective method to treat adenoid cystic carcinoma.

Humans ; Infant ; Job Syndrome ; diagnosis ; immunology ; therapy ; Male

AIM: To investigate cerebrodynamic feature of fear and fright in traditional Chinese medicine theory of emotion through the compiling model of spontaneous electroencephalic sign.METHODS: Using clinical electroencephalography to input electroencephalic wave signs of the patients with fear and fright emotions psychologically into a real computer for image formation, investigating the cerebrodynamic feature.RESULTS: The electroencephalic waves were mainly escape mode for patients with fear emotion; while the electroencephalic waves were mainly condensing mode for patients with fright emotion.CONCLUSION: The emotional states (fear and fright) of human could be presented with physical method.

Recombinant human epidermal growth factor (rhEGF, 20 ng/ml) and Genistein (740 μmol/ml) were added to the cells from 12 cases of human pituitary tumors and co-cultured in vitro. The 3H-thymidine uptake of tumor cells was measured by scintillation counter and tyrosine kinases activity was detected by using immunocytochemical method. The results showed that 3H-thymidine uptake and tyrosine kinases activity were increased in 10 out of 12 cases of human pituitary tumor by EGF and both declined by Genistein. It was suggested that EGF had a strong mitogenic effect on majority of pituitary tumors. Genistein could abolish the effects of EGF promoting the proliferation of the tumor cells, inhibit the growth of the tumor and might be useful in pituitary tumor treatment.

Animals ; Antibodies, Monoclonal ; metabolism ; therapeutic use ; Biomarkers, Tumor ; metabolism ; Breast Neoplasms ; metabolism ; pathology ; therapy ; Drug Delivery Systems ; Epidermal Growth Factor ; immunology ; metabolism ; physiology ; Epithelial-Mesenchymal Transition ; GPI-Linked Proteins ; Humans ; Immunotherapy ; methods ; Intercellular Signaling Peptides and Proteins ; Membrane Glycoproteins ; immunology ; metabolism ; physiology ; Neoplasm Invasiveness ; Neoplasm Metastasis ; Neoplasm Proteins ; immunology ; metabolism ; physiology

Objective:To analyze the effect and safety of blood glucose fluctuation in brittle diabetes treated with intensive insulin therapy combined with sodium-glucose transporter 2 inhibitor.Methods:Ninety-eight patients with brittle diabetes in Shenzhen Longhua District People's Hospital were selected from May 2017 to May 2019. They were divided into two groups by random number table, 49 cases in each group. The control group was treated with intensive insulin therapy combined with saglitine, while the observation group was treated with intensive insulin therapy combined with sodium-glucose transporter 2 inhibitor. Following up for 12 weeks, the plasma glucose and blood glycemic variations, insulin dosage, plasma 8-iso-prostaglandin F2α, hypersensitive C-reactive protein and safety related indicators were compared between the two groups before and after treatment.Results:Compared with the control group, the standard deviation of blood glucose [(1.8±0.5)mmol/L vs (6.5±1.4)mmol/L, t=7.235], large amplitude glycemic excursions [(6.5±1.1)mmol/L vs (17.3±4.7)mmol/L, t=13.446], postprandial glucose excursion [(1.2±0.4)mmol/L vs (9.2±2.0)mmol/L, t=8.921], inter-quartile range [(3.7±1.1)mmol/L vs (12.4±4.2)mmol/L, t=7.003], means of daily difference [(1.5±0.4)mmol/L vs (4.6±0.8)mmol/L, t=4.537] in the observation group were significantly decreased ( P<0.05), and the levels of plasma 8-iso-prostaglandin F2α [(7.8±1.2)ng/L vs (13.6±2.3)ng/L, t=4.882], hypersensitive C-reactive protein [(5.2±1.3)mg/L vs (8.7±1.3)mg/L, t=4.406], insulin dosage [(30.9±10.2)U/d vs (42.3±13.4)U/d, t=5.726] and body mass index [(18.3±1.2)kg/m 2 vs (21.0±2.3)kg/m 2, t=4.135] also decreased significantly ( P<0.05), and the incidence of hypoglycemic events [16.3%(8/49) vs 36.7%(18/49), χ 2=9.697] and severe hypoglycemia [0 vs 14.3%(7/49), χ 2=7.268] decreased significantly ( P<0.05). Conclusions:Combination of dapagliflozin and intensive insulin therapy can significantly improve glucose metabolism in brittle diabetes, and reduce insulin dosage and hypoglycemic events.