CCL21 is originally reported as a CC chemokine mainly expressed in secondary lymphoid tissues. CCL21 can dictate lymphocytes homing in the flow environment,where T cells use integrins stimulated by CCL21 to arrest on endothelial cells,and can act as a chemoattractant for T cells,B cells,mature dentritic cells and natural killer cells strongly. CCL21 can collect activated effector cells and keep them around the tumor. It is also a pivotal molecule for priming T cell responses. Therefore,CCL21 plays a potential role in anti-tumor therapy, and its high expression is responsible for many immune diseases.

Acetazolamide, a sulfonamide-derived medication, is frequently used in the treatment of various types of glaucoma. But this kind of medicine is associated with acute angle closure glaucoma. This paper is a review for the researches of this rare adverse reaction at internal and abroad. It can prompt more ophthalmologists to understand and pay attention to this rare adverse reaction of acetazolamide and sulfonamides derived drugs. Then the doctor will make accurate diagnosis, timely treatment and grasp the right operation opportunity and reduce the occurrence of postoperative complications ultimately.

Mass data is absent for the employment of the graduates in biomedical engineering specialty in China. Questionnaire was carried out in the graduates from 1989 to 2013 in School of Biological Sciences & Medical Engineering of Southeast University, and more than 1 500 questionnaires on the graduate employment were classified, summarized and analyzed to provide instructions for graduate employment guidance and student education in biomedical engineering specialty.

The purpose of this review is to investigate the value of the calcification in differentiating malignant thyroid neoplasm and the molecular mechanisms for the formation of the calcification. Many published reports have proved the presence of calcifications in thyroid neoplasm and calcified nodules in these studies are more frequently malignant than noncalcified nodules. Through viewing the related references, we found that psammoma bodies (PBs), Runx2, osteocalcin, osteopontin, CD44v6 play an important role in the molecular mechanisms in the formation of the calcification in PTC. But further study is required for elucidating the mode of action.
Calcinosis ; diagnosis ; pathology ; Core Binding Factor Alpha 1 Subunit ; metabolism ; Humans ; Hyaluronan Receptors ; metabolism ; Osteocalcin ; metabolism ; Osteopontin ; metabolism ; Thyroid Neoplasms ; diagnosis ; pathology ; Thyroid Nodule ; pathology

Objective: To study collagen distribution and its quantitative variation in t he aortic adventitia of spontaneously hypertensive rats Methods: Sirus red stain was used to observe collagen distribution in aortic adventitia；Chloramine-T oxidation assay was employed to quantitate the collagen of aortic adventitia；Immuno-histochemical staining was adopted to detect type Ⅰ、Ⅲ collagen synthetic characteristic of vascular adventitial fibroblasts. Results: Collagen of rat aoritc wall mainly distributes in adventitia；SHR shows more collagen deposition in adventitia than WKY at the age of 8weeks and 24 weeks；The immuno-histochemical staining of type Ⅰ、Ⅲ collagen in vascular a dventitial fibroblasts is positive. Conclusions: All those results indicate that vascular adventitia ma y be involved in hypertensive vascular remodeling.and vascular adventitial fibroblasts can synthe size collagen type Ⅰ and Ⅲ.

AIM:Effects of anatotensin Ⅱ on type Ⅰ collagen synthesis and its mRNA expansion in cultured vascular adventitial fibroblasts. METHODS: Vascular adventitial fibroblasts (VAF)were isolated, cultured from rat thoracic aorta by explant method. ELISA was used to study type Ⅰ collagen synthesis and competihve RT - PCR was employed to detect its mRNA expression after angiotensin Ⅱ administration. RESULTS: Angiontensin Ⅱ caused a dose dependent increase of type Ⅰ collagen synthesis and its mRN expression in VAF. CONCLUSION: The results support that angiotensin Ⅱ is an important factor controlling collagen metabolism of VAF and VAF may play an im- portant role in vascular remodelling of hypertension.

Two ent-kauranoids were isolated from the ethanol extraction of rhizomes of Canna generalis (Cannaceae), and were purified by various technologies, including silica gel and high performance liquid chromatography, and their structures were determined by modern spectroscopy techniques as (5R,8S,9S,10R,13R)-2-oxo-ent-kaur-15-en-17-oic acid (1) and (4R,5S,8S,9S,10S,13R)-19-hydroxy-ent-kaur-15-en-17-oic acid (2). Compound 1 is a new ent-kauranoid, and compound 2 is obtained from rhizomes of Canna generalis for the first time.

Objective To study characteristic findings and the diagnostic value of MRI in pituitary stalk interruption syndrome (PSIS). Methods Twenty-one patients with PSIS were included. Small field of view (FOV) MR1 scanning and clinic hormone detection were performed in all patients. Moreover, fluid attenuated inversion recovery (FLAIR)T,1 WI with fat-suppression sequence was performed in 8 cases. The appearance on FLAIR T1 WI and T2 WI were recorded. The shape of pituitary stalk and antehypophysis, and the signal intensity of posthypophysis were analyzed simultaneously. Results Growth hormone deficiency (GHD) was confirmed by clinic hormone detection in all 21 cases. The level of basal GH varied from 0. 03 μg,/L to 1.50 μg/L. The peak value under GH provocation was from 0. 13 μg/L to 4. 14 μg/L, and the GH was absolute default in all patients. Seventeen cases of them were combined pituitary hormone deficiency (CPHD), and 4 cases were isolated growth hormone deficiency (IGHD). The height of antepituitary was in the range from 1 mm to 3 mm, and the average value was (1.9±1.2) mm. Pituitary stalk was absent in 18 cases and showed as linear and discontinuous stalk in the other 3 cases. The high signal intensity was invisible in normal position in all cases, the high signal intensity spot in the region of infundibular recess of the third ventricle was shown in 19 cases, while it could not be found anywhere in 2 patients with diabetes insipidus. Conclusion PSIS often shows characteristic appearance on MRI, and a definite diagnosis can be made by using MRI combined with clinic hormone detection.

Objective To study the chemical constituents from the aerial parts of Anabasis aphylla.Methods The chemical constituents were isolated and purified by silica gel column and Sephadex LH-20 column chromatography.Spectroscopic methods such as MS and NMR spectra were used for the structural identification.Results A new caffeate ester, named eicosyl-(Z)-caffeate (1), along with fourteen known compounds was isolated from the EtOAc part.Conclusion Compound 1 is a new compound and compounds 2-13 are isolated from the plants of Anabasis L.for the first time.

Objective To investigate the effect of compound catechu anti-diarrhea ointment on the expression of aquaporin3 (AQP3) in the colonic tissues of diarrhea rat model, and the mechanism thereof. Methods Forty SD rats were randomly divided into four groups:blank control group, model control group, positive control group and compound catechu anti-diarrhea ointment group. Rats were given 6 g blank matrix cream in model control group, 2 mL suspension of berberine in positive control group and compound catechu anti-diarrhea ointment in compound catechu anti-diarrhea ointment group, two times/d for 7 d. The rat model of diarrhea was established by using senna intragastric administration. The water content of feces was measured. The expression of AQP3 in colonic tissues was detected by immunohistochemistry assay. Results The water contents of feces were significantly higher in model control group (64.09±0.41)%than those of other three groups (F=53.879,P＜0.05). There was no significant difference in the water content of feces between compound catechu anti-diar-rhea ointment group (48.83 ± 1.08)%and positive control group (46.87 ± 2.19)%. The AQP3-positive cells were mainly ex-pressed in the intestinal mucosa. The dyeing index was significantly lower in model control group (0.85±0.18) than that of other three groups (F=14.971,P＜0.05). There was no significant difference in the dyeing index between compound catechu anti-diarrhea ointment group (1.30±0.18) and positive control group (1.37±0.14). Conclusion Compound catechu anti-di-arrhea ointment can significantly reduce the water contents of feces, which may be related to the increased AQP 3 expression in colonic tissues.