N". Except the average (Avg)-RNFLT had positive correlation with diopter in severe myopia group (r=0.372, P=0.047), no significant correlation was observed in other parameters among three groups. Conclusion RNFLT in myopia measured by OCT may have its own rule. Avg-RNFLT may have positive correlation with diopter in severe myopia group.

Objective To study the expression-mode and dynamic transmutation of high mobility group box-1 (HMGB1) in hepatocytes of the mouse model with acute hepatic failure and to study the interaction beween HMGB1 and tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β). Methods The mouse model of acute hepatic failure was established by injecting D-galactosamine (D-GalN) and lipopolysaccharide(LPS). Immunohistochemistry SABC method was used to detect the HMGB1 expression at 6 time points. The enzyme linked immunosorbent assay (ELISA) was used to determine serum TNF-α. IL-1β levels. Paired t test was used for statistical analysis. Results The HMGB1 expression was detectable at 2 hours after injection, which dramatically increased over time and peaked at 24 hours after injection. The serum TNF-a level and IL-1β level increased right after injection. The TNF-a level peaked at 8 hours after injection with a maximum value of (473.42±22. 99) pg/mL. The IL-1β level peaked at 2 hours after injection with a maximum value of (724. 49±34. 24) pg/mL. Both cytokine levels slowly decreased after peaking. IL-1β level returned normal with (51. 49±36. 87) pg/mL. Conclusions HMGB1 is one of the most important factors during the development of acute hepatic failure, which can promote the secretion of TNF-α and IL-1β at early stage and be abundantly expressed under the effect of these cytokines at middle and late stages with the result of liver damage. This process is directly correlated with the development and severity of hepatic failure.

At present, filtration surgery remains an important treatment of glaucoma, and filtering bleb fibrosis is the main cause for treatment failure. Filtering bleb fibrosis is a common fiber hyperplastic disease, and it relates to the activation and proliferation of fibroblasts and the excessive production of extracellular matrix ( ECM) such as collagen protein. The most frequently-used drugs for filtering bleb fibrosis in clinic are 5-fluoro-2,4 (1h, 3h) pyrimidinedione ( 5 - Fu ) and mitomycin ( MMC ) . Although they are effective in some degree, they also have some serious side effects which restrict their clinical use. Triptolide ( TPL) is a major active component of the medicinal plant, tripterygium wilfordii hook. f. ( TWHF) . TPL has multiple pharmacological activities including immunosuppressive, anti- inflammatory, anti- cancer and anti-fertility activity. Reviewing related literatures published in recent ten years, we confirmed that TPL seemed to possess a pharmacological activity in treating filtering bleb fibrosis. Since it has three major functions:1. inhibit the activation and proliferation of fibroblasts and the excessive production of collagen protein; 2. alleviate the inflammatory reaction after surgical wound to suppress fibrous scar formation; 3. TPL has a protective effect on retinal ganglion cells ( RGCs ) . We further find that TPL's anti-fibrosis activity mainly results from that it inhibits TGF-β/Smad,NF-κB and PI3K/AKT signal transduction pathway. This comprehensive analysis about the feasibility of Triptolide's role in treating filtering bleb fibrosis after the filtration surgery of glaucoma can help us develop new drugs for filtering bleb fibrosis and exploit TPL's clinical value on some level.

Objective In order to evaluate the quality and performance of chlorine dioxide disinfectant,it is necessary to develop a set of methods to determine the components in chlorine dioxide disinfectant.Methods Iodometry,malonic acid iodometry,improved malonic acid iodometry and five-step iodometry were employed to determine the components including ClO_2, Cl_2,ClO_2~-,and ClO_3~-in chlorine dioxide disinfectant,the advantages and disadvantages of the test methods were compared.Results Using iodometry,the liquid stabilized chlorine dioxide products activated by hydrochloric acid,the content of ClO_2 was 20.23 mg/ml that was accord with the content which was marked in the product label.Using malonic acid iodometriy,the liquid stabilized chlorine dioxide contents of ClO_2 was 19.99 mg/ml,Cl_2 was 0.35 mg/ml,in which a little chlorine was detected.Using improved malonate iodometry and five-step iodometry,the activation rates of the liquid stabilized chlorine dioxide products were 88.0% and 75.6% respectively,ClO_2 and ClO_2~-in the liquid stabilized chlorine dioxide products could be distinguished.Conclusion Active chlorine dioxide and residual ClO_2~-can be detected by using iodometriy and improved malonic acid iodometry,but iodometriy and malonic acid iodometry can not.

Objective To study the analysis of ritodrine hydrochloride in comparison with Magnesium Sulfate expect application effect in the treatment of placenta previa.Methods In Wenling Maternal and Child Health Care Department from January 2014 to December 2016 were 100 cases of expectant treatment of placenta previa patients as the research object in the course of the study, were randomly divided into control group and experimental group two were 50 cases each.The control group was treated with the Magnesium Sulfate treatment, patients in the experimental group of ritodrine hydrochloride.A comparative analysis of the experimental group and the control group of patients with successful pregnancy, prolonged pregnancy and neonatal weight and other indicators, including the adverse reactions of patients.Results After the treatment,the number of success cases in experimental group was 48 cases, the success rate was 96%,the number of cases to 42 cases stopped bleeding during pregnancy, to extend the time for(16.2±12.0)days, neonatal weight(3.21±0.35)kg.The number of cases of successful pregnancy control group of patients was 41 cases, the success rate was 82%,the number of cases to 36 cases stopped bleeding during pregnancy, to extend the time for(12.2±10.2)days, neonatal weight(2.39±0.48)kg.Available, the experimental group success time extension of pregnancy and neonatal weight were significantly better than the control group, with statistical difference(P<0.05).Can be obtained, the experimental group and the control group, the incidence of adverse reactions were statistically significant.Conclusion Ritodrine hydrochloride and Magnesium Sulfate expect application effect in the treatment of placenta previa compared the clinical effect of ritodrine hydrochloride, can effectively improve the indicators for further promotion and application, has the clinical significance.

Medicinal materials research and development of new drug of traditional Chinese medicine (TCM) research is the premise and foundation of new drug research and development, it throughout the whole process of new drug research. Medicinal materials research is one of the main content of the pharmaceutical research of new drug of TCM, and it is also the focus of the new medicine pharmaceutical evaluation content. This article through the analysis of the present problems existing in the development of TCM research of new drug of TCM, from medicine research concept, quality stability, quality standard, etc are expounded, including medicine research idea value medicine study should focus on the important role and from the purpose for the top-level design of new drug research problem. Medicinal materials quality stability should pay attention to the original, medicinal part, origin, processing, storage, planting (breeding), and other aspects. Aspect of quality standard of medicinal materials should pay attention to establish the quality standards of conform to the characteristics of new drug of TCM. As the instruction of TCM new drug research and development and the scientific nature of the review, and provide the basis for medicinal material standards.
Biomedical Research ; methods ; standards ; China ; Drug Stability ; Drug Storage ; Drugs, Chinese Herbal ; chemistry ; standards ; Medicine, Chinese Traditional ; methods ; standards ; Quality Control

The strategies for automatic extraction of key words from medical news were put forward by adding the MeSH terms into the general classification table in combination with the length of MeSH terms and location-weigh-ted MeSH terms.The key words randomly selected from 100 papers reporting medical news on 10 Websites were in-dexed and verified by machine indexing.The extraction accuracy was 0.34 and the recall rate was 0.30, showing that the strategies can be used for automatic extraction of key words from medical news.

Objective To analyze the clinical effect of minimally invasive percutaneous nephrolithotomy titanium laser lithotripsy(MPCNL)on upper ureteral calculi.Methods According to digital table,68 patients with upper ureteral calculi were randomly divided into the study group and control group,cach group in 34 cases.The study group received the MPCNL treatment,the control group received extracorporeal shock wave lithotripsy(ESWL)therapy. The therapeutic effects were compared.Results The success rate of gravel,stone clearance rates of the study group were 91 .1 8% and 91 .1 8%,which were higher than 70.59%,67.65% of the control group,there were significant differences between the two groups(χ2 =4.660,5.757,P =0.031 ,0.0164).Conclusion Percutaneous nerve titanium laser lithotripsy for upper ureteral stones can achieve good therapeutic effect.

Objective To investigate the effect of dexamethasone combined with clomiphene on serum sex hormone levels in patients with polycystic ovary syndrome. Methods 80 pregnancies with polycystic ovary syndrome treated in our hospital from January 2013 to December 2015 were selected and they were randomly divided into observation group and control group with 40 cases in each group. The observation group was given dexamethasone combined with clomiphene, while the control group was given simple clomiphene. Compared the curative effect and the change of the levels of progesterone and estradiol before and after treatment for three months. Results After treatment, the total effective rate of the observation group was 95.00%, higher than the total effective rate of the control group was 72.50%, the difference was statistically significant (P<0.05), compared the levels of progesterone(P), estradiol(E2), luteinizing hormone(LH) and LH/FSH in the observation group and the control group, the difference was statistically significant (P<0.05), after treatment, both levels were higher than before treatment. The total ovulation rate of the observation group was 90.00%, higher than the total ovulation rate of the control group was 72.50%, the difference was statistically significant (P<0.05). The level of progesterone and estradiol in serum of polycystic ovary syndrome treated by dexamethasone combined with clomiphene had a obvious positive correlation (r=0.746, 0.812, 0.731 and 0.796, P<0.05). Conclusion Dexamethasone combined with clomiphene treatment of polycystic ovary syndrome than using only clomiphene treatment is good, which can also improve serum hormone levels in patients, effectively improve the patient's endocrine abnormalities.

Objective The aim of this study was to investigate the distribution of body weight related index and to explore the relationship between weight related index and cardiovascular diseases in rural residents in Yunnan Province. Methods By random cluster sampling method,rural residents were selected in Yunnan Province. The data were collected by face-to-face questionnaire and medical examination. Results 4070 rural residents in Yunnan participated in this study. The survey results showed that gender,age,education level and ethnicity affected both the distribution of body weight related index and low body weight,overweight,obesity,and central obesity(P < 0.05). Compared with low body weight,non overweight,non obesity and non central obesity group,non low body weight,overweight,obesity and central obesity group had higher prevalence rate of hypertension(P < 0.01). Among central obesity group,the prevalence rate of coronary heart disease was higher than non central obesity group(P < 0.01). Conclusion The distribution of body weight related index in rural residents of Yunnan province showed some special characteristic. The overweight,obesity,and central obesity were related to cardiovascular diseases. Change unhealthy living habits or behavior as well as improving their cultural quality have effects on controlling body weight related index and help to reduce the prevalence rates of overweight,obesity and central obesity which can further reduce the prevalence rates of cardiovascular diseases.