1.Assay of curcumol in Baofukang Suppository by capillary GC
Yusheng WANG ; Xiaogen WANG ; Tiexin TANG
Chinese Traditional Patent Medicine 1992;0(06):-
AIM: To develop the assay of curcumol in Baofukang Suppository by capillary GC. METHODS: GC Internal standard method was used to determine curcumol in Baofukang Suppository with capillary column. RESULTS: In the assay, curcumol and the internal standard are both well separated from other substances. A good linear relationship is obtained in the range of 0.1~0.5?g(r=0.9995). The RSD of precision, repeatability and recovery are all less than 3%. CONCLUSION: The method is accurate, reliable, and repeatable, suitable for the assay of the preparation and curcuma oil.
2.Comparative Study of Several Pattern Recognition Methods in the Identification of Volatile Oils of Tradition-al Chinese Medicine by Infrared Spectroscopy
Xinhua QIU ; Tiexin TANG ; Yan LIU ; Meizhu WU ; Xiongsi TAN ; Kelin GAN ; Weisheng YAO
China Pharmacy 2015;(21):2986-2988
OBJECTIVE:To compare the performance of several pattern recognition methods in the identification of volatile oils of traditional Chinese medicine(TCM)by infrared spectroscopy. METHODS:The volatile oils of several Lonicera and Citrus TCM were determined by infrared spectroscopy. All samples of infrared spectrum were classified by hierarchical clustering,K-mean clustering,artificial neural networks,and support vector machine. RESULTS:The results of hierarchical clustering and K-mean clus-tering were ineffective. Methods of artificial neural networks and support vector machine achieved correct classification rate of 100%. CONCLUSIONS:Artificial neural networks and support vector machine can be combined with infrared spectroscopy to cre-ate chemometric fingerprinting for the identification of volatile oils of TCM.
3.Identification of Lonicerae japonicae flos Volatile Oils by Fourier-transform Infrared Spectroscopy
Yan LIU ; Tiexin TANG ; Xinhua QIU ; Meizhu WU ; Xiongsi TAN ; Kelin GAN ; Weisheng YAO
Chinese Journal of Information on Traditional Chinese Medicine 2013;(11):63-65
Objective To set up a method for identification of Lonicerae japonicae flos volatile oils using Fourier-transform infrared spectroscopy. Methods The volatile oils of Lonicerae japonicae flos and Lonicerae flos was extracted by steam distillation combined with continuous liquid-liquid extraction with hexane. An oil film was prepared for Fourier-transform infrared spectroscopy scanning by dropping the volatile oils solution on the KBr disc and evaporating the solvent. The obtained infrared spectrum was treated by baseline removing and median filter smoothing. The spectral data within 1800-850 cm-1 was selected as the characteristic spectrum for hierarchical cluster analysis. And the volatile oils of Lonicerae japonicae flos and Lonicerae flos were discriminated by the result of hierarchical cluster analysis. Results Enough volatile oils were extracted for obtaining Fourier-transform infrared spectrum from small amount of Lonicerae japonicae flos. The method developed in the study was able to discriminate Lonicerae japonicae flos volatile oils from Lonicerae flos volatile oils. Conclusion The method can be used for identification of Lonicerae japonicae flos volatile oils.