Objective To evaluate the effects of TGF ? 1, EGF, bFGF, KGF on the cell growth of androgen independent prostate cancer cell line PC 3M in vitro. Methods We cultured androgen independent prostate cancer cell line PC 3M in vitro and added TGF ? 1, EGF, bFGF, KGF to the medium at different concentrations of 1, 5,10 and 50 ng/ml. Brdu incorporation method was used to determine the status of cell proliferation. Results The absorbance ( A ) value of the control group PC 3M was 0 759. Seventy two hours after adding the growth factors separately, the inhibition was enhanced with the increasing of concentrations. At the dosage of 50 ng/ml, A value decreased to 0 400(TGF ? 1),0 455(EGF),0 532(bFGF),and 0 491(KGF),respectively, showing significant difference compared to the controls. When the mixture of 1 ng/ml EGF with 0 5 ng/ml or 5 ng/ml TGF ? 1 was added into the medium, A values were 0 522 and 0 299, respectively. Conclusions All these four growth factors inhibited the proliferation of PC 3M cell line in a dose dependent manner. TGF ? 1 and EGF have no interaction to the cell proliferation.

Objective To investigate whether AGEs stimulate human lymphocytes to produce cytokines and whether the immune response to AGEs stimulation is the same or not in diabetics and normal subjects.Methods Venous blood was collected from 55 normal subjects and 50 diabetic patients.The blood sample was mixed in 1640 culture medium with 10?g/ml PHA or 20?g/ml AGEs respectively for 24 hours incubation.TNF-? and IL-6 of the medium were determined by ELISA test kit.Results After 24 hours incubation,AGEs-induced TNF-? production in diabetic patients and normal subjects was 473.20?66.93pg/ml vs 254.62?35.14pg/ml ( P 0.05).There was no relation of higher cytokine production with course of diabetes,glycemia levels,body weight and sex.Conclusion The above results demonstrated that (1) AGEs do stimulate human lymphocytes to produce TNF-? and IL-6;(2)The immune response to AGEs and PHA was more sensitive in diabetics,at least partially,than that in normal subjects;(3)It seems that the sensitive response to AGEs stimulation has no relation with course of diabetes,glycemia levels,body weight and sex in tested individuals.Our findings imply the possibility that immune response to AGEs of diabetic individual can be studied by this method.The sensitive response to AGEs stimulation in diabetes may be an independent risk factor involved in diabetic complications.

Objective To evaluate the clinical significance and choicing antibiotics of serum procalcitonin (PCT) in acute fe-ver.Methods To make a retrospective analysis of 170 cases of febriledisease from January 2013 to January 2014.According to clini-cal causes of fever, the cases were divided between infectious fever group and non -infected group.The infectious group was divided by bacterial(A group) and viral infectious(B) groups.The bacterial group(A group) was divided by bloodstream infectious (A1) and fo-cal infectious(A2) groups.Tests of blood routine and CRP and PCT before or used antibiotics were analyzed and compared between the different groups.Results The PCT serum concentration of bacterial infectious gloup was higher than the viral infectious group and non-infected group( P <0.01).There wasn't significant difference in the viral infectious group and non -infected group( P >0.05). In bacterial infectious gloup, the area under ROC of PCT was 0.950 and CRP was 0.943, if PCT≥ 0.25ng/ml was regard as the cut-ff value, the sensitivity was 91.6% and the specificity was 93.3% ; if PCT≥ 0.5ng/ml was regard as the cut-ff value, the sensitivity was 95.2% and the specificity was 77.6%.Conclusions PCT tests are helpful to identify bacterial infection in patients with infec -tious fever .The more serious the higher PCT level , the earlier using antibiotics ,it is the most in bloodstream infection.There is an important significance for choicing antibiotics by the guidance of PCT ≥ 0.25ng/ml.

Objective:To probe the influence ofrelated index ofmetabolic syndrome on TCM clinical syndrome.Methods:A statistical factor analysis was made to related index ofmetabolic syndrome in 1 231 random people ofphysical examination.To probe the influence ofsyndrome differentiation offive zang on common factor.Results:The results indicated that there were close relationship between liver and lipid,heart and blood pressure, kidney and fasting plasma glucose.There were neither evident mutuality between abdominal circumference and syndromes offive zang,nor between related index ofmetabolic syndrome and the lung or the spleen.Conclusion:Regulation ofthe liver,heart and kidney in TCM may be the key to prevention and treatment ofmetabolic syndrome.

To explore relationship of body mass index (BMI), waist circumference (WC), blood lipid level with non-alcoholic fatty liver (NAFL) to provide evidence for its early intervention. Methods Data of 13 532 people living in Beijing undergone regular physical examinations at Beijing Hospital, including liver-ultrasonic scanning, measurements of blood lipid, height, weight, waist circumference and BMI during January to December 2007 were statistically analyzed. Results Totally, 4 807 people were diagnosed as NAFL by B-type ultrasonic scanning, with a mean age of (54 ± 14) years and median age of 53 years. Prevalence of NAFL significantly increased with changes in body building (normal weight, overweight and obesity) and age groups (X2 = 1640.394, P<0.01 ). There was statistical significance in prevalence of NAFL between groups with normal weight and overweight at varied ages ( X2 = 172.618 and 31.928, respectively, P < 0.01 ), with the highest at ages of 51 - 60 years. No statistical difference in prevalence of NAFL between varied age groups was found in those with obesity (X2 = 6.194, P =0.402). Mean of WC, BMI, serum levels of triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C) were significantly higher in NAFL group than those in those without NAFL, but mean serum level of high-density lipoprotein cholesterol (HDL-C) was significantly higher in the former than that in the latter ( P < 0.05 ). Prevalence of dyslipidemia was significantly higher in NAFL group than that in those without NAFL ( X2 = 112. 974 - 895. 500, respectively, P < 0. 01 ), with the highest of hypertriglyceridemia, accounting for 54. 36 percent. Prevalence of NAFL was significantly higher in those with larger WC than that in those with normal WC in the normal weight and overweight group (X2 = 120.982 and 45.327, respectively, P<0.01). Results of logistic regression analysis showed that WC, BMI, TG and LDL-C all were risk factors for NAFL both in men and women, and HDL-C was a protective factor for NAFL only in women. Conclusions Overweight, obesity and dyslipidemia closely correlate with occurrence of NAFL Control of body weight and blood lipid level are essential for decreasing prevalence of NAFL.

Objective To explore the direct association between metabolic syndrome (MS) and gamma glutamyltransferase (GGT) or alanine aminotransferase (ALT) in Beijing men and women.Methods We investigated the medical check-up data from the 14,204 physical examination population (male 9524,female 4680) of year 2008.A cross-sectional study was carried out to explore the direct association between MS and GGT or ALT.MS was defined by the Chinese Diabetes Society (2004).Chisquare test was performed to analyze the categorical variables.The receiver operating characteristic curve (ROC) was plotted to obtain area under curve (AUC) and the cutoff point of GGT and ALT.Results The prevalence of MS was significantly higher in subjects with the highest quartile of GGT or ALT than the subjects with the lowest quartile of GGT or ALT.For male,the prevalence of MS is 7.57％ in the lowest GGT quartile and 29.04％ in the highest GGT quartile.For female,the prevalence of MS is 1.28％ in the lowest GGT quartile and 17.86％ in the highest GGT quartile.For male,the prevalence of MS is 9.27％ in the lowest ALT quartile and 21.88％ in the highest ALT quartile.For female,the prevalence of MS is 3.52％ in the lowest ALT quartile and 14.70％ in the highest ALT quartile (P ＜0.01).The optimal cutoff points of GGT to diagnose MS were 31 U/L in men and 18 U/L in women.The optimal cutoff points of ALT to diagnose MS were 23 U/L in men and 19 U/L in women.Conclusions The prevalence of MS increases with the increase in serum levels of GGT or ALT even through the normal range of GGT or ALT in Chinese men and women.

Objective: To investigate the correlation between the presence of a crown (CR) or a proximal restoration (PR) and furcation involvement (FI) on molars. Methods: Data were collected from 237 teeth in 44 patients who had restored and non-restored molars with or without FI.The clinical examinations were conducted on the molars including CR,PR,FI, mobility(MO), endodontic treatment(EN) and probe of periodontal attachment level(PAL). Results: Molars with CR or PR had a significantly highter percentage of FI (P0.05) when compared to molars without restorations. Conclusion: The molars with crowns or restorations involving the proximal tooth surfaces have a highter prevalence of FI and more periodontal attachment lose than molars without restorations.

Ultrasonography can clearly show pelvic anatomic structures,as well as the location and length of mesh after pelvic floor reconstructive operation.The consistency of ultrasonic diagnosis of severe pelvic organ prolapse with pelvic organ prolapse quantitation is good.Furthermore,ultrasonography can provide imaging basis for evaluating the safety and efficacy of pelvic floor reconstruction.The progresses of ultrasound in diagnosis of severe pelvic organ prolapse and postoperative evaluation of pelvic floor reconstruction were reviewed in this article.

Thyroid nodule is a common diseases of neck,which property is usually identified by ultrasound examination.Color doppler ultrasonography can reflect the distribution of blood vessels and blood flow within the nodules.And with development of new technologies,three-dimensional ultrasound increase the accuracy of identification.Three-dimension power doppler ultrasound (3D-PDUS) can reflect blood flow information within the nodule space,which is more accurate than two-dimensional ultrasound and it can be used to evaluate the blood flow in nodules qualitatively and quantitatively,provide a new reference for clinical diagnosis of thyroid nodule.