1.Effects of curcumin on NT-pro-BNP, IL-6, IL-10 and pulmonary function in patients with sepsis induced by lung injury
Lihua ZHANG ; Tielin ZHANG ; Jin DU ; Jingguo ZHANG
Chinese Journal of Biochemical Pharmaceutics 2015;37(7):89-91
Objective To investigate effect of curcumin on NT-pro-BNP, IL-6, IL-10 and pulmonary function in patients with sepsis induced by lung injury.Methods 40 patients with purulent septic lung injury were collected in the hospital and divided into control group and experimental group according to different treatment, 20 cases in each group.The control group were given conventional treatment and the experimental group were given curcumin capsule on the basis of control group for a consecutive treatment of one weeks.After treatment, the serum levels of NT-pro-BNP, IL-6, IL-10 and pulmonary function were detected of two groups.ResuIts Compared with control group after treatment, serum IL-6 and IL-10 levels of patients in experimental group decreased significantly ( P<0.05 ) , serum NT-pro-BNP levels in experimental group decreased significantly ( P<0.05 ) , vital capacity (VC), maximal voluntary ventilation (MVV), and forced expiratory volume in one second (FEV1) levels in experimental group increased significantly (P<0.05).ConcIusion Curcumin could significantly reduce serum NT-pro-BNP, IL-10 and IL-6 levels in patients with lung injury, improve the pulmonary function, and has a guiding significance for clinical use.
2.Three-dimensional CT angiography with volume rendering for the dignosis of multiple intracranial aneurysms
Bing FANG ; Tielin LI ; Jianmin ZHANG ; Chuanzhi DUAN ; Qiujing WANG ; Qingping ZAO
Chinese Journal of Cerebrovascular Diseases 2004;1(1):36-42
Objective:To evaluate the importance of 3D-CTA with volume rendering for the diagnosis of multiple intracranial aneurysms. Methods: Axial source images were obtained by helical CT scanning and reconstruction of 3D-CTA images was done by volume rendering technique in conjunction with multiplanar reformation. Results: In the past one year,there were 10 patients diagnosed as having multiple intracranial aneurysms by 3D-CTA and altogether 24 aneurysms were visualized,including 10 small aneurysms(≤5mm.Three dimensional CT angiography with volume rendering demonstrated aneurysms very well and provided useful information concerning the site,shape,size and spatial relationship with the surrounding vessels and bone anatomy. Conclusion: Three-dimensional CT angiography with volume rendering is a quick,reliable,and relatively noninvasive method for diagnosing multiple intracranial aneurysms.It delineates detailed aneurysmal morphology,and provides useful information for planning microsurgical approaches.
3.Relationship between TRX1 and JAB1 in acute myelogenous leukemia
Lingyun HUI ; Qiuju SHEN ; Ting WANG ; Shan MENG ; Chenyang LU ; Tielin YANG ; Wanggang ZHANG ; Fuling ZHOU
Journal of Xi'an Jiaotong University(Medical Sciences) 2017;38(1):62-65,70
ABSTRACT:Objective To detect the expressions of thioredoxin (TRX1)and c-jun-activation-domain binding protein-1 (JAB1)in patients with acute myelogenous leukemia (AML)and healthy controls,and measure the TRX1 level in AML patients at different stages for evaluating its clinical significance.Methods The expressions of TRX1 and JAB1 in leukemia samples were analyzed by RT-PCR and Western blot at mRNA and protein levels, respectively.The correlation between TRX1 and JAB1,and the relationship between the gene expression and peripheral blood leukocytes count were also analyzed.Furthermore,serum TRX1 was measured by ELISA.Results TRX1 and JAB1 expressions at both mRNA and protein levels were obviously upregulated in leukemia patients (P<0.05). TRX1 was positively related to JAB1 in both newly diagnosed and recurrent AML patients.And high levels of TRX1 and JAB1 expressions were associated with white blood cell (WBC)counts in AML patients (P<0.05).Moreover, abundance of TRX1 in serum was significantly greater in AML patients,especially in the patients with recurrent AML,than in healthy donors (P<0.05).Conclusion There is a positive correlation between the expressions of TRX1 and JAB1 ,which is closely related to the occurrence and progression of AML.
4.Angiographic features and clinical significance of kinking of extracranial internal carotid artery
Yingguang ZHANG ; Jixiang ZHU ; Guifu LI ; Xiaoxin BAI ; Wenyan ZHU ; Shengping HUANG ; Tielin LI ; Hao LIN ; Wangchi LUO
Chinese Journal of Radiology 2009;43(2):181-184
Objective To investigate the clinical manifestations, angiographic features and clinical significance of kinking of extracranial internal carotid artery (ICA). Methods The clinical and radiological data of 21 patients with kinking of extracranial ICA were retrospectively reviewed in our hospital from April 2003 to July 2007. Fisher exact test was performed. Results Of the 21 patients, 7 hod no clinical symptoms, the other 14 showed manifestations of cerebral iachemia with varying degree. One of the characteristic clinical manifestations that neck rotation or specific positions of head and neck might induce the occurrence of clinical symptoms was found in 5 cases. The whole-brain coverage DSA accurately showed the location of kinking of extracranial ICA and the degree of vascular stenosis. In patients with α < 66%, 80% > α≥ 66% and α≥ 80%, clinical symptoms were found in 3 out of 5,7 out of 10 and 4 out of 6 patients, respectively. Fisher exact test revealed that the positive rates of clinical symptoms in three groups had no significant difference (P > 0. 05). Conclusion Kinking of extracranial ICA is a frequent vascular morphologic variation, and it is also a kind of potential disease. The whole-brain coverage DSA is a relatively reliable method to detect this variation.
5.Diagnosis and treatment of a gastric cancer patient with hemorrhage and new cerebral infarc-tion
Changlong CHEN ; Yuhai LIU ; Yongqing ZENG ; Guannan SHI ; Hanlin WU ; Mengqi ZHANG ; Chaobing SUN ; Tielin ZHANG ; Junsong ZHANG
Chinese Journal of Digestive Surgery 2023;22(S1):82-86
Gastric cancer with hemorrhage and cerebral infarction is a serious complica-tion with poor prognosis in clinic. Although the incidence rate is extremely low, the fatality and disability rates are very high. In addition, the opposition in treatment between the two complica-tions increases the difficulty of clinical diagnosis and treatment. The authors report the diagnosis and treatment of a gastric cancer patient with hemorrhage and new cerebral infarction, in order to to provide reference for related treatments.
6.Mechanical thrombectomy versus Intra-arterial Thrombolysis in Patients with Stroke Caused by Acute ce-rebral Arterial Occlusions:A Single-center study
Zhaohui MA ; Guifu LI ; Jinsong YOU ; Jixiang ZHU ; Wangchi LUO ; Yingguang ZHANG ; Jianwen GUO ; Fajun CHEN ; Yao SHI ; DaoJin XUE ; Foming ZHANG ; Longlong WEN ; Wenyan ZHU ; Zhenyun GU ; Yan HUANG ; Tielin LI
Chinese Journal of Nervous and Mental Diseases 2015;(7):406-411
Objective To investigate the safety and efficacy of mechanical thrombectomy (MT) compared with In?tra-arterial Thrombolysis (IAT) treatment in patients with severe acute ischemic stroke (AIS) caused by large cerebral ar?tery occlusion. Method The patients with AIS caused by large cerebral artery occlusion and underwent MT or IAT from 2005 May to 2014 May was included. A retrospective analysis was conducted on the onset to emergency(OTE)time, emergency to acupuncture(ETA)time, acupuncture to recanalization (ATR) time, stroke severity as measured by the Na?tional Institutes of Health Stroke Scale (NIHSS) score, and site of arterial occlusion on magnetic resonance angiography (MRA). A comparison was made between MT and IAT patients in rates of recanalization, symptomatic intracranial bleed?ing (SIB), mortality, and functional outcome. Three-month favourable outcome was defined as a modified Rankin Scale (mRS) score≤2. Result One hundred and two AIS patients were treated with MT and 50 with IAT. There was no differ?ence between MT and IAT groups with regard to demographics, onset NIHSS score (13.37±6.95 vs. 12.70±6.11;P=0.572) and discharge NIHSS score (8.40 ± 6.69 vs. 7.53 ± 7.28, P= 0.522) and the change of NIHSS score (3.87 ± 7.14 vs. 4.26 ± 5.42, P=0.766). There were significantly differences between MT and IAT groups in the OTE time (Median 300 min vs. 120 min,Z=-5.704,P=0.000) , ATR time (Median 30 min vs. 65 min,Z=-5.011,P=0.001) ,recanalization (91.2%vs. 60.0%,P =0.01),the rate of AIB(21.7% vs. 36.0%,P =0.046),3-month mortality (16.6% vs. 26.0%,P =0.043). The above parameters were better in MT group than in the IAT group. There were no significant differences between MT and IAT groups in the rate of SIB (12% vs. 16%,P =0.055), the NIHSS change(Median 3 vs. 4,Z =-0.236,P =0.823) and mRS score on 90d ( 48.2%vs. 46.0%, P=0.823). MT patients had significantly higher percentages of stent use (22.5%vs. 8%,P=0.018) . The Recanalization for ICA(81.8%vs. 55.6%,P=0.048),BA(93.1%vs. 55.6%,P=0.032)and MCA( 97.5% vs. 60.0%,P =0.026)was higher in MT group than in IAT group .The SIB rate for ICA(13.8% vs. 33.3%,P =0.000),BA(13.8%vs. 33.3%,P=0.000)was lower in MT group than in IAT group . The mortality rate of was significant?ly lower in MT than in IAT group for MCA (2.5%vs. 20.0%,P=0.000) . the good outcome rate for BA was higher in MT group than in IAT group(41.3%vs. 22.2%,P﹤0.01). Conclusions Compared to IAT,MT can provide broader time win?dow,higher recanalization rate and better outcome in patients with severe acute ischemic stroke (AIS) caused by large ce?rebral artery occlusion.
7.Effect of Light and Temperature on Photosynthetic Fluorescence Parameters and Volatile Oil Content of Atractylodes lancea
Meiyu CAO ; Kai SUN ; Binbin YAN ; Sheng WANG ; Tielin WANG ; Yan ZHANG ; Lanping GUO
Chinese Journal of Experimental Traditional Medical Formulae 2022;28(20):108-115
8.Identification of Arisaematis Rhizoma and Pinelliae Rhizoma by PCR-RFLP
Jiancai XIAO ; Binbin YAN ; Jian YANG ; Jiahui SUN ; Tielin WANG ; Xiufu WAN ; Kai SUN ; Chao JIANG ; Yan ZHANG
Chinese Journal of Experimental Traditional Medical Formulae 2023;29(6):194-201
ObjectiveIn recent years, with the sharp decline of wild resources in Arisaematis Rhizoma and Pinelliae Rhizoma and the immaturity of medicinal cultivation technology, their adulterants have appeared frequently in the market, and the main identifying characteristics have mostly disappeared in the circulation of medicinal materials. Therefore, there is an urgent need to establish a molecular identification method that can quickly and effectively identify the specificity of Arisaematis Rhizoma and Pinelliae Rhizoma. MethodAfter comparison of the rbcL sequences of Arisaematis Rhizoma,Pinelliae Rhizoma, and their adulterants, the specific enzyme cleavage sites Hae Ⅲ and Dra Ⅰ of Arisaematis Rhizoma and Pinelliae Rhizoma, respectively, were selected and identified by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP). The main system conditions of PCR-RFLP reaction were established and optimized, and their durability and the ability to detect genuine, adulterants, and mixed counterfeits were investigated. ResultThe PCR-RFLP identification method of Arisaematis Rhizoma and Pinelliae Rhizoma was established. After specific primer amplification, Arisaematis Rhizoma and Pinelliae Rhizoma could be digested by Hae Ⅲ and Dra Ⅰ-restricted endonucleases respectively, at annealing temperature of 54 ℃, the number of cycles of 35, and the amount of DNA template of 3-30 ng, producing two fragments or small cut fragments with a single band between 100-250 bp, whereas the mixed counterfeits were not cleaved and both showed a band at 250 bp. The method is highly accurate in identifying adulterants and mixed counterfeits of Arisaematis Rhizoma or Pinelliae Rhizoma. ConclusionThe PCR-RFLP method developed in this study allows for the rapid identification of Arisaematis Rhizoma and Pinelliae Rhizoma.