ABSTRACT:Objective To detect the expressions of thioredoxin (TRX1)and c-jun-activation-domain binding protein-1 (JAB1)in patients with acute myelogenous leukemia (AML)and healthy controls,and measure the TRX1 level in AML patients at different stages for evaluating its clinical significance.Methods The expressions of TRX1 and JAB1 in leukemia samples were analyzed by RT-PCR and Western blot at mRNA and protein levels, respectively.The correlation between TRX1 and JAB1,and the relationship between the gene expression and peripheral blood leukocytes count were also analyzed.Furthermore,serum TRX1 was measured by ELISA.Results TRX1 and JAB1 expressions at both mRNA and protein levels were obviously upregulated in leukemia patients (P<0.05). TRX1 was positively related to JAB1 in both newly diagnosed and recurrent AML patients.And high levels of TRX1 and JAB1 expressions were associated with white blood cell (WBC)counts in AML patients (P<0.05).Moreover, abundance of TRX1 in serum was significantly greater in AML patients,especially in the patients with recurrent AML,than in healthy donors (P<0.05).Conclusion There is a positive correlation between the expressions of TRX1 and JAB1 ,which is closely related to the occurrence and progression of AML.

Objective:To explore the effect of hederagenin (HE) on the proliferation of bladder cancer cell T24 in vitro and in nude mice.Methods:Human bladder cancer cells T24 were divided into control group and experimental group. The experimental group was cultured with DMEM medium containing 25μg/mL hederogenin, and CCK8 was used to detect cell proliferation ability. Nude mice were divided into a control group and an experimental group and injected with T24 cells. The cells of the experimental group were injected with ivy sapogenin at 30 mg/kg every other day. The protein of T24 cells and tumor mass was extracted to detect the expression of p-JNK/JNK and p-p38/p38.Results:After the bladder cancer cells T24 were treated with hederagenin, the CCK8 results showed that the cell proliferation ability of the experimental group was significantly decreased ( P<0.05) . The expression levels of p-JNK in experimental group and control group were 0.21±0.06 and 0.89±0.15, respectively, and the expression levels of p-p38 were 0.38±0.09 and 1.44±0.26, respectively. The expressions of p-JNK and p-p38 were up-regulated (all P<0.05) . In vivo, it was found that after treatment with ivisaponin, the volume of tumor mass were 1192.07±250.92μm 3 in the subcutaneous tumor experimental group and 2280.50±600.1μm 3 in the control group, and the mass were 0.65±0.29g and 1.62±0.38g, respectively. The mass and volume of the experimental group were decreased (all P<0.05) . We extracted mass proteins, and western blotting results showed that the expression levels of p-JNK in the experimental group and control group were 0.38±0.08 and 1.03±0.19, respectively, and the expression levels of p-p38 were 0.71±0.12 and 1.36±0.25, respectively. The expressions of p-JNK and p-p38 were up-regulated (all P<0.05) . Conclusion:Hederagenin inhibits the proliferation of bladder cancer in vitro and in nude mice through the JNK/p38 MAPK signaling pathway.

ObjectiveIn recent years， with the sharp decline of wild resources in Arisaematis Rhizoma and Pinelliae Rhizoma and the immaturity of medicinal cultivation technology， their adulterants have appeared frequently in the market， and the main identifying characteristics have mostly disappeared in the circulation of medicinal materials. Therefore， there is an urgent need to establish a molecular identification method that can quickly and effectively identify the specificity of Arisaematis Rhizoma and Pinelliae Rhizoma. MethodAfter comparison of the rbcL sequences of Arisaematis Rhizoma，Pinelliae Rhizoma， and their adulterants， the specific enzyme cleavage sites Hae Ⅲ and Dra Ⅰ of Arisaematis Rhizoma and Pinelliae Rhizoma， respectively， were selected and identified by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP）. The main system conditions of PCR-RFLP reaction were established and optimized， and their durability and the ability to detect genuine， adulterants， and mixed counterfeits were investigated. ResultThe PCR-RFLP identification method of Arisaematis Rhizoma and Pinelliae Rhizoma was established. After specific primer amplification， Arisaematis Rhizoma and Pinelliae Rhizoma could be digested by Hae Ⅲ and Dra Ⅰ-restricted endonucleases respectively， at annealing temperature of 54 ℃， the number of cycles of 35， and the amount of DNA template of 3-30 ng， producing two fragments or small cut fragments with a single band between 100-250 bp， whereas the mixed counterfeits were not cleaved and both showed a band at 250 bp. The method is highly accurate in identifying adulterants and mixed counterfeits of Arisaematis Rhizoma or Pinelliae Rhizoma. ConclusionThe PCR-RFLP method developed in this study allows for the rapid identification of Arisaematis Rhizoma and Pinelliae Rhizoma.