Objective:To study the relationshop between cell adhesion molecules CD 62 P expression and biological behavior of rectal cancer.Methods:The levels of CD 62 P expression was observed in 40 cases of rectal cancer using SP immunohistochemistry staining.Rusults:The rate of CD 62 P expression was 65%(26/40) in rectal cancer.CD 62 P expression was more intensive in rectal cancer with infiltrated serosa and lymph node metastasis ( P

Objectives:In order to study the immune function after postoperative parenteral nutrition enriched with arginine in patients with gastrointestinal carcinoma. Methods:Forty patients who underwent radical operation of gastrointestinal carcinoma were randomly divided into experimental group( n= 20),in which patients received TPN enriched with arginine,and control group (TPN, n =20).At day 1 before operation,day 1 and day 8 after operation,IgG,IgA,IgM,T cell subgroups.NK cell activity and IL 2 were dectected. Results:The immune function of patients with gastrointestinal carcinoma was depressed at different levels.At day 1 after operation,IgG,IgM,IgA,CD4 +,CD4 +/CD8 +,Nk cell activity and IL 2 decreased more significantly than those before operation.The immunefunction recovered rapidly and exceeded the level before preoperation in experimental group.The difference was significant( P

Objective To establish a method for the quality control of Semen Cassiae.Methods A high performance liquid chromatographic method was developed to establish the fingerprint of Semen Cassiae,and 32 samples from various batches of Semen Cassiae were analyzed.Cluster analysis and principal component analysis were applied to study HPLC fingerprint and chemical pattern recognition.Results The fingerprint of Semen Cassiae was set up.Conclusion The method could be used for the quality control and comprehensive evaluation of Semen Cassiae.

Objective To establish a method for quality control of Radix Paeoniae Alba.Methods A high performance liquid chromatographic method was developed to establish the fingerprint of Radix Paeoniae Alba and 47 samples from various batches were analyzed.Cluster analysis and principal component analysis(PCA) were applied to study on HPLC fingerprint and chemical pattern recognition method. Results The result of PCA and cluster analysis showed that the samples were divided into two types.The HPLC fingerprinting of Radix Paeoniae Alba,showing 10 characteristic peaks,was established from 47 batches of Radix Paeoniae Alba.Conclusion The method provides an academic reference for the quality control of Radix Paeoniae Alba.

Objective To establish a method for quality control of Rhizoma Chuanxiong.Methods A high performance liquid chromatographic method was developed to establish the fingerprint of Rhizoma Chuanxiong,and 23 samples from various batches were analyzed.Cluster analysis and principal component analysis were applied to studying HPLC fingerprint and chemical pattern recognition.Results The fingerprint of Rhizoma Chuanxiong was set up.Conclusion The method provides an academic reference for controlling the quality of Rhizoma Chuanxiong.

Objective To study the separation and purification technology of iridoid glycosides and xanthones from Swertia mussotii by macroporous resin.Methods The dynamic adsorption and desorption characteristics of macroporous adsorption resins HPD-300,HPD-400,HPD-600,AB-8,DM-301,and D-101-Ⅰ for swertiamarin and swertianolin were investigated.On the base of the investigation,the better macroporous adsorption resin was chosen to finish the experiments.Results The adsorption and elution characteristics of HPD-300 used to separate and purify iridoid glycosides and xanthones were very good.In the course of adsorption,the amount of used adsorption is 0.9 g dried medicinal herb/mL resin,with about 8 pH value of adsorption solution,and the volumetric rate is 2 BV/h; In the course of elution,the resin column chromatography was eluted gradiently with 7 BV 20% and 5 BV 70% EtOH after removing impurities with water.The elution rates were both more than 90%,and the contents of iridoid glycosides and xanthones were both up to 50% in the two dried fractions of 20% and 70% ethanolic elutions.Conclusion HPD-300 is an ideal resin with the best enrichment for separating and purifying both iridoid glucosioles and xanthanoes simultanously.

ObjectiveTo investigate the effects and indication of intermittent catheterization(IC) by relative safe bladder capacity(RSBC) to treat vesical-ureteral reflux(VUR).MethodsThere were 23 male complete SCI patients, aged between 7～45 years, including 15 patients without vesicao-ureteral reflux injuried 1～3 months as control group, 3 patients in mild group affected only ureter, and 5 patients in severe group affected not only ureter but also the pelvis. All the patients were taken intermittent catheterrization according to their own RSBC companied with supersonic device. The times of IC per day is that the average total urine volume one day was divided by RSBC. The observation time is over 2 years. During the oberservation some medicine was used to enlarge some patients bladder capacity.ResultsThere were no reflux in the control group; the reflux in the mild group improved a lot, some even disappeared, and there were no change in the severe group.ConclusionIC by RSBC is effective to prevent and cure vesical-ureteral reflux in some SCI patients.

Objective To explore the effect of lymph node metastases in gastric cancer on vagniae vasorum of gastric vessels,in order to provide evidence for the surgical treatment of gastric cancer.Methods The study included 90 specimens of left and right gastric arteries from 47 patients who underwent radical gastrectomy for carcinoma from January 2004 to 2006.All specimens were dealt with frozen section method,then they were stained with HE and immunohistochemical method respectively.Results Metastatic cancer cells were not found inside vagina vasorum in stage Ⅰ specimens.Cytokeratiu positive of tumor cens in or on vagina vasorum was showed on 25 slices from 10 tumors.Among them,2 slices from 1 tumor belonged to stage Ⅱ.8 slices from 3 tumors belonged to stage Ⅲ and 15 slices from 6 tumors belonged to stage Ⅳ.There was significant differences among cytokeratin positive rate in different stages of gastric cancer(P＜0.01).Conclusion Vaginae vasorum dissection is recommended when clearing lymph node around arteries in radical gastrectomy for carcinomas in stage Ⅱ above.

Human T-cell leukemia virus type 1 (HTLV-1) is a retrovirus demonstrated to be associated with human disease. Infection by the HTLV-1 can cause T-cell leukemia (ATL) in adults. HTLV-1 bZIP factor (HBZ) is a viral protein encoded by the minus strand of the HTLV-1 provirus. Among the regulatory and accessory genes of HTLV-1, HBZ is the only gene that remains intact and which is expressed consistently in all patients with ATL. Moreover, HBZ has a critical role in the leukemogenesis of ATL. Here, we review the function of HBZ in the oncogenesis of HTLV-1 and its molecular mechanism of action.
Animals ; Basic-Leucine Zipper Transcription Factors ; genetics ; metabolism ; Carcinogenesis ; HTLV-I Infections ; pathology ; virology ; Human T-lymphotropic virus 1 ; genetics ; metabolism ; Humans ; Leukemia, T-Cell ; pathology ; virology ; Retroviridae Proteins ; genetics ; metabolism

Objective To observe the effect of Boari bladder muscle flap surgery in nephron sparing surgery for patients with middle and lower ureteral carcinoma. Methods Totally 80 patients with middle and lower ureteral carcinoma underwent surgery in our hospital from March 2009 to March 2012 were selected and divided into the control group and the observation group,with 40 cases in each group. Patients of the control group were treated with ureteral carcinoma radical prostatectomy while patients of the observation group were treated with Boari bladder muscle flap surgery,and all of them were followed up for 3 years after operation. Observed the renal function,renal tumor markers and the level of inflammatory symptoms of the 2 groups before the operation and 3 months after the operation,and compared the long-term progno-sis of the 2 groups. Results There was no significant difference between the 2 groups in renal function index before operation. The level of BUN,Scr,blood urea and serum uric acid index in the 2 groups were all increased compared with those before operation,but the increase of the control group was significantly higher with statistically significant differences (P<0. 05). The tumor markers CA19-9, Survivin and in-flammatory marks WBC,NEC%,IL-6,hs-CRP had no significant difference before operation,and all the level of the 2 groups were decreased after operation,but there was still no difference between the 2 groups (P>0. 05). The survival rate 1 year,2 years and 3 years after operation also had no significant difference between the 2 groups (P>0. 05). Conclusion The use of Boari bladder muscle flap surgery in nephron sparing surgery for patients with middle and lower ureteral carcinoma can effectively maintain the renal function of patients without affecting the radical and long-term prognosis.