Objective: To explore the relationship between lifestyle and ankle-brachial blood pressure. Methods: Ankle-brachial blood pressures of 2121 adults from all over China were measured with self-developed KD-991 portable ankle-brachial blood pressure simultaneous detecting instrument. They were divided into smoking group and no smoking group, heavy drinking group and moderate drinking group, exercise group and lack of exercise group and comparisons of abnormal ankle-brachial index (ABI, ≤0.9 or≥1.3) rate between paired two groups were performed. Results: Rate of abnormal ankle-brachial index (ABI, ≤0.9 or≥1.3) in smoking group (15.3%), heavy drinking group (13.6%) and lack of exercise group (10.5%) were significantly higher than those of no smoking group (7.9%), moderate drinking group (7.5%) and exercise group (7.4%) respectively, P<0.05 all. Conclusion: Unhealthy lifestyle is related to abnormal ankle-brachial blood pressure and it is very good to prevent occurrence of atherosclerosis , early discover and effectively control its development using ABI to guide people to change unhealthy lifestyles.