Objective To observe the effect of underground part-obturated environment on human health. Methods 12 testees were chosen to undergo the experiment of 6 days in the part-obturated situation. The microclimate blood routine immunological items and the symptoms were investigated. Results In the part-obturated environment the average temperature was 15.2 ℃ the humidity was 75.5% the velocity of the wind was 0.06 m/s. Among the testees one was found had higher WBC number three had higher lymphocytes number. During the experiment the average value of CD3+ CD8+ and B cell were 60.05% 26.04% and 10.41% respectively the NK cell was higher compared with the controlbefore the experiment the differences were significant P

[Objective]To provide quantitative data of atlantoaxial pedicle for its surgical screw internal fixation by imageology measurement and improve the success rate of the surgical treatment.[Method]The examinations ofCR,DRX,MRI and 64-row CT were performed in each patient preoperatively,and the atlantoaxial pedical screw entry points and screw entry angles were then measured by PACsee system.[Result]The atlas pedicle screw entry points were localized position which its distance to the left of the atlas pedicle midline was(19.93?1.32) mm,and to the right of the atlas pedicle midline was(19.16?1.30)mm.The screw entry angles to the inside of the atlas pedicle were localized position which its distance to the left of the atlas pedicle midline was(23.72?2.09)?,and to the right of the atlas pedicle midline was(23.35?1.91)?.The screw entry angle to the head of the atlas pedicle was(9.00?1.20)?.The axis pedicle screw entry points were localized position which its distance to the left of the axis pedicle midline was(13.14+0.82) mm,and to the right of the axis pedicle midline was(13.85?0.79)mm.The screw entry angles to inside of the axis pedicle were localized position which its distance to the left of the axis pedicle midline was(24.52?1.26)?,and to the right of the axis pedicle midline was(20.42?1.42)?,The screw entry angle to the head of the axis pedicle was(25?3)?.48 patients were taken treatment with atlantoaxial pedicle surgical screw intemal fixation.Among these patients,there were 35 males and 13 females with a mean age 43.60 years old(ranged 22 to 61 years old),22 patients with type II old odontoid fracture,12 patients with odontoid nonunion and 14 patients disruption of the transverse ligament.The x-ray and CT scans of all post-surgery patients could prove the atlas were completely reduced,axis odontoid fracture had good reduction and bony fusion were achieved after 10.6 months.The JOA evaluation standards showed 31 patients were excellent,14 patients were good,2 patients were fair and a patient was poor,excellent and good ratio was 93.57%.[Conclusion]The imageology measurement quantitative data of atlantoaxial pedicle could guide effectively the screw internal fixation surgery.

Abdomen/surgery* ; Humans ; Laparoscopy/methods* ; Pelvic Floor/surgery* ; Perineum/surgery* ; Proctectomy ; Rectal Neoplasms/surgery*

Objective To investigate the correlation between inflammatory cells, body temperature changes, and TCM syndromes of deep venous thrombosis (DVT) patients in acute and subacute phases. Methods The data of age, gender, body temperature, blood routine, venous ultrasonography, and four diagnostic information of 130 DVT patients in acute and subcute phases were collected and analyzed in a cross-sectional study. The correlation between inflammatory cells and the changes of body temperature and TCM syndromes were analyzed. Results Among 130 DVT patients, 37 patients had damp-heat syndrome, 64 patients had blood stasis and dampness syndrome, and 29 patients had qi stagnation syndrome. Neutrophils increased most obviously in blood stasis and dampness syndrome (P<0.05), which had close correlation with the skin redness (OR=1.287, 95%CI: 9.412-21.247). The mononuclear cells increased most obviously in the damp-heat syndrome, which has close correlation with nouhof (OR=7.364, 95%CI:1.189–45.603), high skin temperature (OR=6.683, 95%CI:1.791–24.938), skin tightness (OR=6.107, 95%CI:1.423–26.203) and weakness (OR=3.302, 95%CI: 1.002–9.169). The temperature rising was the most common in the damp-heat syndrome, and the increase of mononuclear cells was the most common one. Conclusion DVT is often accompanied with elevated levels of inflammatory cells and body temperature. Damp-heat syndrome has close correlation with body temperature and mononuclear cells increasing. Dampness and blood stasis syndrome and neutrophils are closely related.

Objective To evaluate the effect of SvO2-guided early goal directed fluid therapy on hemodynamic and oxygen dynamics in septic shock pig model. Methods Twelve Bama miniature pigs (male, 21-24 kg) were equally randomized into 2 groups, group C and group G. Septic shock was induced by intravenous infusion endotoxin. Group C received hemodynamic support aiming central venous pressure at 8 to 12 mmHg, urinary output 0.5 mL/kg per hour, and mean arterial pressure greater than 65 mmHg. Group G maintained SvO2 greater than 0.65 in addition to the above indicators. The interventions lasted 6 h and at T0-T8 (0, 60, 120, 180, 240, 300, 360, 420 and 480 min) recorded temperature, hemodynamic and oxygen dynamics indexes for each group, and recorded 6 hours for accumulated liquid volume, vascular active drug, and changes of urine. Results There were no significant differences in mean arterial blood pressure (MAP), heart rate (HR) and systemic vascular resistance index (SVRI) at each time point between group G and group C ( P>0.05). Values of CI and CVP were increased at T4-T8 in group G (P<0.05). Values of MPAP and PVRI were decreased at T8 in group G (P<0.05). Values of SvO2 were increased at T3-T8, O2ER were decreased at T3-T8 (P<0.05), DO2 were increased at T4-T8 (P<0.05), Lac were decreased at T5-T8, andΔp(CO2) was decreased at T8 in group G (P<0.05). There were no significant differences in values of VO2 at T1-T8 between two groups (P>0.05). The amount of intravenous infusion and urine volume were more and the amount of norepinephrine was less in animals of group G (P<0.05). The dosage of dobutamine was more in animals of group G, and which was not used in animals of group C. Conclusion SvO2 guided fluid therapy is more effective than conventional treatment to stabilize hemodynamics and oxygen kinetics, which is characterized by the increased cardiac output, increased oxygen supply, normal oxygen uptake rate and good tissue perfusion.

OBJECTIVE: To establish a genetic diagnosis method for a novel MSH2 mutation. METHODS: A specific primer on the mutated site of MSH2 was synthesized and PCR was conducted using the specific primer and another downstream primer. PCR products were electrophoresed and then the carriers with the novel gene mutation of the carriers or non-carriers were identified. RESULTS: MSH2 in a hereditary nonpolyposis colorectal cancer family were successfully found. CONCLUSION The method is effective and simple for genetic diagnosis of the novel mutation in MSH2.
Base Sequence ; Colorectal Neoplasms, Hereditary Nonpolyposis ; genetics ; DNA Mutational Analysis ; methods ; Female ; Humans ; Male ; Molecular Sequence Data ; MutS Homolog 2 Protein ; genetics ; Pedigree ; Point Mutation ; Polymerase Chain Reaction

Objective To investigate the etiology and clinical symptoms of dural arteriovenous fistula (DAVF) and explore its diagnostic and treatment approaches. Method Of the 19 patients with DAVFs, 17 were examined by the microcather technique under close monitoring by cerebral digital substraction angiography (DSA), and were treated by embolization method with several prepared materials. The other 2 cases were managed by way of compression of the affected carotid artery. Results Of the 19 patients, 10 were cured, and the symptoms of 4 were significantly improved. Four patients showed gradual improvement after treatment, and 1 died. Conclusion The embolization method combined with compression of the affected carotid artery was effective in managing dural arteriovenous fistula.

Objective To evaluate the therapeutic effects of mitral valve repair for the treatment of the anterior learlet prolapse of mittsl valve.Methods From November 1998 to October 2007,210 patients with severe anterior leaflet prolapse of mitral valve underwent valve repair.The condition of valve was preoperative,intraoperative,and postoperative assessed with echocardiography.Results Edge-to-edge repair technique was used in 134 cases(63.8%).The cardiac function was NYHA class Ⅰ in 168 cases and class Ⅱ in 40 cases after operation.Patients were followuped for 1-150(25.7±29.0)months,two patients(0.95%)died of postoperative low cardiac output syndrome.Echocardiography examination indicated that the mean JP2postoperative left atrial diameter was(37.7±9.2)mm against the preoperative value of(47.5±12.7)mm(P＜0.05),the mean postoperative left ventricular end-diastolic diameter was (51.7±7.9)mm against the preoperative value of(67.7±10.3)mm(P＜0.05),the mean postoperative left ventrlcular ejection fraction was(62.2±3.2)%against the preoperative value of(52.2±6.4)%(P＜0.05),and the mean preoperative regurgitation area was(10.4±4.1)cm~2 against the postoperative value of (4.1±1.7)cm~2(P＜0.01).Conclusions Optimal outcome was achieved by appropriate edge-to-edge technique or other mitral valve repair techniques for anterior leaflet prolapse of mitral valve.Edge-to-edge technique is a reliable and efficient surgical technique.

Objective To investigate the etiology and clinical symptoms of dural arteriovenous fistula (DAVF) and explore its diagnostic and treatment approaches. Method Of the 19 patients with DAVFs, 17 were examined by the microcather technique under close monitoring by cerebral digital substraction angiography (DSA), and were treated by embolization method with several prepared materials. The other 2 cases were managed by way of compression of the affected carotid artery. Results Of the 19 patients, 10 were cured, and the symptoms of 4 were significantly improved. Four patients showed gradual improvement after treatment, and 1 died. Conclusion The embolization method combined with compression of the affected carotid artery was effective in managing dural arteriovenous fistula.

BACKGROUNDGenetic mechanisms contribute to blood pressure regulation. This study investigated whether glutathione peroxidase (GPx-3) tag single nucleotide polymorphisms (SNPs) are associated with hypertension in the rural areas of Fuxin county, Liaoning province, China.

METHODSIndigenous Fuxin Han people participated, 523 unrelated hypertensives and 547 controls were recruited. All tag SNPs of GPx-3 gene were selected. We estimated SNP allele frequency in DNA pools with pyrosequencing.

RESULTSBefore Bonferroni correction, C allele frequency for rs8177417 was significantly higher in hypertensives than those in controls (23.4% vs. 19.3%, P = 0.014); T allele frequency for rs3828599 was significantly lower in hypertensives than those in controls (35.6% vs. 40.8%, P = 0.009). However, when a Bonferroni correction for multiple testing was applied, only the polymorphisms rs3828599 of GPx-3 gene was associated with hypertension (P = 0.045, OR: 0.833, 95%CI: 0.695 - 0.998).

CONCLUSIONThe polymorphism of rs3828599 of GPx-3 gene might be associated with hypertension in rural Han Chinese from Fuxin, Liaoning.

Aged ; Female ; Gene Frequency ; genetics ; Genetic Predisposition to Disease ; genetics ; Glutathione Peroxidase ; genetics ; Humans ; Hypertension ; genetics ; Male ; Middle Aged ; Polymorphism, Single Nucleotide ; genetics