Chinese materia medica of hemostasis are frequently used in clinic,the author has the honor to follow Professor LI Tie to study the clinical knowledge,and benefits lots from it.This article sums up the clinic experience of LI Tie in using hemostatic Chinese herbs,such as Xianhecao,Baimaogen,Qiancao,Sanqi,Baiji,Zonglvtan,Diyutan,Xueyutan,Aiye,Puhuang and so on.Besides using hemostatic method,the methods of removing heat to cool blood,reinforcing spleen and invigorating qi,promoting blood flow for removing blood stasis are used according to the cause of disease.The treatment principle are treating secondary symptoms for emergency and paying more attention to strengthening the body resistance.There are the unique characteristics in compatibility of drug pairs,and the cliniceffect is very good.

OBJECTIVE:To investigate the drug treatment of hospitalized patients with depression in our hospital during 2008. METHODS: In cross section study, the medical records which in accordance with ICD-10 diagnostic criteria for depression disorders in our hospital during 2008 were retrieved, then the utilization of psychotropic drugs was analyzed and summarized. RESULTS: The utilization rate of new antidepressants including selective serotonin reuptake inhibiter (SSRI), selective serotonin and noradrenaline reuptake inhibiter (SNRI), noradrenaline and specific serotonin antagon (NaSSA) reached 89.9%, among which sertraline accounted for the largest proportion (22.5%). New antidepressants combined with atypical antipsychotics took up a big proportion, particularly quetiapine (39.2%). CONCLUSION: New antidepressants become the mainstream drug in the treatment of depression. Drug combination occupies a big proportion, especially combining with atypical antipsychotics. The safety of drug combination need to be further explored.

ObjectiveTo investigate the protective effect of calcium channel blocker, verapamil against human hepatic ischemia-reperfusion injury.MethodsThe patients undergoing lobe/segment hepatectomy were injected with 5?mg/2ml verapamil through vena gastroepiploica dextra before blocking hepatic portal.ResultsThe levels of free calcium ions in the hepatic cell and serum enzyme from the test group increased significantly than those of the control group. The degree of hepatic pathological injury was lower and the hepatic function restored earlier in test group.ConclusionCalcium channel blockers administered by hepatic portal vein before blocking hepatic portal have an important protective effect against human hepatic ischemia-reperfusion injury.

This paper introduces a multi-function adjustable anesthetic laryngoscope.The laryngocope or called tongue-spatula can be adjusted freely in length between12-14cm and in ante-posterior diameter between 2.8-4.5cm according to patients＇condition.An oxygen -supply pipe and suction tube are settled on the bottom and side of tongue-spatula to provide oxygen and absorb phlegm simultaneously on exposure of glottis.The tongue-spatula not only fits for normal adults but also for those with high prominewtia,short neck,long epiglottis, absent of incisor teeth as well as tall and fatty persons.It has got satisfying result in 29 cases of medical experiment.

Objective To investigate the methods for localization of the lung segments on three-dimentional (3D) pulmonary perfusion SPECT/CT imaging.Methods Twelve healthy adults were recruited into this study.Lung segments were defined based on the anatomical criteria of CT on transaxial, sagittal and coronal images, including apical, posterior, anterior segments in right upper lobe ( RUL), lateral, medial segments in right middle lobe ( RML), superior, anterior basal, lateral basal, posterior basal segments in right lower lobe (RLL); apical posterior, anterior, superior lingual, inferior lingual segments of left upper lobe ( LUL), superior, medioanterior basal, lateral basal, posterior basal segments of left lower lobe (LLL).Results (1) Eleven typical sections were selected on transaxial SPECT pulmonary perfusion imaging:sternoclavicular joint, supra-arch vessels, aortic arch, azygous arch, right upper lobar bronchus, left upper lobar bronchus, middle lobar or lingual bronchus, basal trunk of bronchus, lower lobar vein, superior and inferior basal vein and basal vein.(2) Twelve typical sections were defined on sagittal imaging:left hilum, bifurcation of left principal bronchus, septa of left pulmonary artery, cardiac apex, the first and second sections left to cardiac apex; right hilum, bifurcation of intermite bronchus, interlobar artery, bifurcation of interlobar artery, the second and third sections right to right atrium.(3) Seven typical sections were defined on coronal imaging:sternoclavicular joint, ascending aorta, bifurcation of pulmonary artery, bifurcation of trachea, intermite bronchus, basal vein and thoracic aorta.Conclusion The developed method was able to localize lung segments on pulmonary perfusion SPECT imaging.

Objective:To study the expression and relationship of Pin1 and CyclinD1 in adult papilloma of larynx,and the effect of both in laryngeal papilloma'S canceration.Method:Ninety-two cases of paraffin section with immunoperoxidase(SP)staining method was used to detect the distribution of Pin1 and CyclinD1 in 10 cases of laryngeal normal epithelial tissue,39 cases of laryngeal papilloma,27 cases of laryngeal papilloma with middle,severe atypical hyperplasia and 16 cases of laryngeal carcinoma.Result:The distribution of Pin1 and CyclinD1 increased gradually from laryngeal normal epithelial tissue tO laryngeal carcinoma(P＜0.05);No difference of the expression of CyclinD1(not including Pin1,for Pin1,P=0.009)was found between laryngeal papilloma and laryngeal papilloma with middle,severe atypical hyperplasia(P＞0.0125),but there had significant difference of the expression of Pin1 and CyclinD1 among the rest groups;There was significantly direct correlation between the expression of Pin1 and CyelinD1(P＜0.05).Conclusion:The hyper-expressions of Pin1 and CyclinD1 may play a key role in laryngea1 papilloma's malignant change.Pin1 up-regulating the expressions of cyclinD1 possibly participate in its malignant change.

Hair follicle stem cells located bulge region of hair follicle,had characteristics of all adult stem cells,including slow-cycling,undifferentiated.They also had the abilities of self-renew and proliferation in vitro.CD34,K15,K19 and Nestin might be the makers of the hair follicle stem cells.They could be induced to differentiate to neurons,glial cells,keratinocytes,smooth muscle cells,melanocytes In vitro and neurons,melanocytes in vivo.There were many signals in regulating the hair follicle stem cells involved Wnt signal,BM Psignal and NFATc1 etc.

Animals for model of osteoporosis involve rat, mouse, rabbit, beagle dog, minipig, sheep, etc. The types of model include aged related model, ovariectomized model, orchietomied model, drug treated model, abolition degeneration model, and dietary bone loss. The rat with ovariectomized model is used widely. Biochemical determination, bone mineral density measurement, bone histomorphorphormetry and bone biomechanics are used to judge the formation of experimental osteoporosis.

Genetic polymorphism of the C1R subcomponent of human first complement component was in-vestigated in 360 unrelated Chinese individuals using isoelectric focusing followed by immunoblotting.Six common and four rare were detected. The allele frequencies were as follows: C1R * 1 = 0. 5181,C1R ? 2=0. 5291,C1R * 3= 0. 1472 and C1R * R(C1R * 5,C1R * 6,C1R * 12 and C1R * 13) = 0.0056. The distribution of allotypes fitted the Hardy-Weinberg equilibrium. This complemlement sys-tem is a useful genetic marker for anthropological studies and forensic science practice.

Objective To study the inhibitory effects of modification c-myc antisense oligodeoxynucleotide (ASODN) on the growth of renal carcinoma cell line (GRC-1) and cell cycle. Methods We transfected c-myc antisense oligodeoxynucleeride in renal carcinoma cell line to investigate the effect of c-myc ASODN on it. Results ① By MTT assay,we found that the c-myc ASODN significantly inhibited the growth of carcinoma cell, compared with sense and mismatch ODN. The growth-inhibition rate of 12 ?mol?L -1 c-myc ASODN group of renal carcinoma cell was 47% at 48 hour, and the inhibitory effect increased with the increase of ASODN dosage and changed with ASODN time. ② The measurements by flow cytometry proved that ASODN mainly blocked the G 1 to S of the cycling of renal carcinoma cell. Conclusion c-myc ASODN can inhibit the growth of renal carcinoma cells with sequence specificity and cycling specificity.