OBJECTIVE:To compare antidiuretic activity of Ootheca Mantidis before and after processing,and to explore the best medicinal part and mechanism of Ootheca Mantidis. METHODS:96 rats were randomly divided into blank group,model group,positive group,Ootheca Mantidis group,Ootheca Mantidis stir-fried with salt group,steamed Ootheca Mantidis group, crude product eggs and egg shell groups,processed product eggs and egg shell groups,with 8 rats in each group,12 groups in to-tal. Except blank group,other groups were given adenine 250 mg/kg,ig,for 4 weeks to induce kidney-yang and diuresis model. From third week,Ootheca Mantidis crude drug group and processed Ootheca Mantidis group were all given relevant medicine 0.11 g(crude drug)/ml i.g,and crude product eggs and egg shell groups and processed product eggs and egg shell groups were given rel-evant medicine,ig,once a day,by mass ratio of eggs to egg shell(cude drug 1∶2.4,salt stir-fried product 1∶1.7,steamed prod-uct 1∶2.1)for consecutive 4 weeks. The urinary volume,body weight,renal index and the serum contents of ADH and ALD were all determined. RESULTS:Compared with blank group,body weight and serum content of ADH and ALD decreased in model group,while renal index and urinary volume increased(P<0.05). After 4 weeks of treatment,compared with model group,body weight and serum content of ADH increased in Ootheca Mantidis groups,while urinary volume and renal index decreased (P<0.05);serum content of ALD increased in treatment groups;there was statistical significance in the serum content of ALD in those groups except Ootheca Mantidis group,Ootheca Mantidis stir-fried with salt group and steamed Ootheca Mantidis group (P<0.05);except for ALD,those index were in descending order of steamed Ootheca Mantidis group>Ootheca Mantidis stir-fried with salt group>Ootheca Mantidis group,and steamed Ootheca Mantidis shell group had best exchange. CONCLUSIONS:The an-tidiuretic activity of Ootheca Mantidis has been enhanced after processing. The egg shell of steamed Ootheca Mantidis is main me-dicinal part. To increase the serum content of ADH might be one of the main mechanism of arresting polyuria.

AIM:To establish the method for quality control of Huisheng Diyidan Tablet(Eupolyphagea seu Steleophaga;Radix Angelicae sinensis,Sanguis Draconis,etc.). METHODS: Radix Angelicae sinesis,Eupolyphaga seu Steleophaga,Chinnabaris,Moschus,Boswellia carterii were identified by microscope.Sanguis Draconis was identified by TLC,Pyritum was identified by general identified reaction;The content limitation of cinnabaris(HgS) was determined by titration.Ferulic acid content of Radix Angelicae sinensis was determined by HPLC. RESULTS: Every part of the tablet was identified,the content limitation of cinnabaris(HgS) and ferulic acid content of Radix Angelicae sinensis were determined. CONCLUSION: The method showed that the characteristic for identification is distinct and highly specific,quantitative assay is simple,accurate,reliable and can be used for quality control of Huisheng Diyidan Tablet effectively.

OBJECTIVE:To establish a method for the contents determination of ginsenoside Rg1,ginsenoside Re,ginsenoside Rb1 and geniposide in Panax ginseng processed by Gardenia jasminoides. METHODS:HPLC was performed on the column of Agi-lent TC18 with mobile phase of acetonitrile-0.1% phosphoric acid,(gradient elution,ginsenoside Rg1,ginsenoside Re,ginsenoside Rb1)and acetonitrile-water(13∶87,V/V,geniposide)at a flow rate of 1.0 ml/min,the detection wavelength was 203 nm for ginsen-oside Rg1,ginsenoside Re,ginsenoside Rb1 and 238 nm for geniposide,column temperature was 40 ℃,and injection volume was 20 μl. RESULTS:The linear range was 0.418-3.762 μg for ginsenoside Rg1(r=0.999 8),0.270-2.430 μg for Re(r=0.999 8), 0.398-3.582 μg for ginsenoside Rb1(r=0.999 7)and 0.606-3.030 μg for geniposide(r=0.999 7);RSDs of precision,stability and reproducibility tests were less than 2.0%;recoveries were 97.86%-101.47%(RSD=1.29%,n=6),96.21%-100.11%(RSD=1.42%,n=6),96.24%-100.48%(RSD=1.57%,n=6)and 97.76%-102.44%(RSD=1.71%,n=6). CONCLUSIONS:The meth-od is simple with good precision,stability and reproducibility,and can be used for the contents determination of ginsenoside Rg1, ginsenoside Re,ginsenoside Rb1 and geniposide in P. ginseng processed by Gardenia jasminoides.

AIM:To research the chemical modification from berberine hydrochloride to berberrubine in Cortex Phellodendri before and after processing.METHODS:Berberrubine was isolated by column chromatography and the contents of berberine hydrochloride and berberrubine in different processed Cortex Phellodendri were determined by HPLC.RESULTS:With increacing processing temperature and time,part of berberine hydrochloride became berberrubine in processed Cortex Phellodendrin,and berberrubine content decreased under the condition of high temperature operation or overtime processing.CONCLUSION:The experimental data shows that the processed Cortex phellodendri has the maximum average content of berberrubine as processing temperature is maintained as 180 ?C for 40 min.

Objective:To investigate the optimal extraction and dripping pills preparation of total terpene constituents in platycladi seed. Methods:The extraction processes of ultrasound, reflux, microwave and homogenization were investigated by single factor tests. The effects of extraction time, temperature, solid-liquid ratio and extraction times on the extraction of total terpene constituents in platy-cladi seed were investigated by orthogonal tests. Orthogonal tests were used to investigate the effects of the ratio of polyethylene glycol 6000 to poloxamer 188, the ratio of drug to matrix, the temperature of liquid and the dropping process on the quality of dropping pills. Results:The homogeneous extraction was used, and the optimum extraction conditions were as follows: the extraction time was 10 min, the temperature was 80℃, the solid-liquid ratio was 1 :10, and extraction time was once. The best preparation process of drop-ping pills was as follows:the ratio of polyethylene glycol 6000 to poloxamer 188 was 5 :1, the ratio of drug to matrix was 1 :3, the temperature of liquid was 80℃ and the dropping distance was 10 cm. Conclusion: The method of homogeneous extraction is simple, which can break the materials and extract the active ingredients simultaneously. The preparation process of dropping pills is reasonable and feasible, which is easy for the production and application.

OBJECTIVE:To optimize the processing technology for Stemonae radix fried with honey. METHODS:Firstly,sin-gle factor test of processing technology for Stemonae radix fried with honey was investigated with the amount of added water,infil-trating time,processing time and temperature as factors,using property and total alkaloid content as indexes. Then,the test was de-signed by orthogonal test;the processing technology was optimized by comprehensive weighted mark method with the amount of added water,processing time and temperature as factors,using total alkaloid content and anti-cough activity for mice as indexes;validation test was conducted. RESULTS:The optimal technology was as follows as 12.5 g honey dissolved with 10 g water for each 100 g Stemonae radix,at 140 ℃,processing for 6 min. In validation test,average content of total alkaliods was 0.77%(RSD=1.5%,n=3);average anti-cough activity was 91.20%(RSD=1.2%,n=3). CONCLUSIONS:The optimized process is simple,stable and easy,and provides reasonable trial reference for the formulation of processing technology for Stemonae radix fried with honey.

Objective To establish the fingerprints analysis of the methanol extracts of nutmeg,and study quality uniformity of nutmeg in different areas.Methods A ZORBAX EclipseXDB-C18 (4.6 mm?150 mm,5 ?m) column was used.The mobile phase consisted of methanol-acetonitrile-water (25∶35∶40),the flow rate was 1 mL/min,the column temperature was 30 ℃,the detective wavelength was 270 nm.Dehydrodiisoeugenol was used as reference compound.Results Fingerprint of nutmeg was established,consisted of l7 common peaks.The similarity of fingerprints was over 0.9.Conclusion The fingerprints of nutmeg in different areas have no differences.This method is accurate,reliable and provides a scientific basis for the quality control of nutmeg.

AIM:To research the chemical modification from berberine hydrochloride to berberrubine in Cortex Phellodendri before and after processing.METHODS:Berberrubine was isolated by column chromatography and the contents of berberine hydrochloride and berberrubine in different processed Cortex Phellodendri were determined by HPLC.RESULTS:With increacing processing temperature and time,part of berberine hydrochloride became berberrubine in processed Cortex Phellodendrin,and berberrubine content decreased under the condition of high temperature operation or overtime processing.CONCLUSION:The experimental data shows that the processed Cortex phellodendri has the maximum average content of berberrubine as processing temperature is maintained as 180℃ for 40 min.

Chinese medicinal decoction pieces were the mainly form for traditional Chinese medicine (TCM) treatment,which means TCM prescription drugs.Any processed (heated or unheated) pieces are known as prepared pieces,which include both raw and cooked pieces.Based on differences of the clinical application of processed pieces,this paper summarized three traditional processing theories,such as the raw and prepared effect difference theory,processing accessories action theory,and pharmaceutical theory.The key points were focused on how different processing methods affect the properties of Chinese herbs,such as four natures and five flavors,floating and sinking,attributive channel,tonifying or purging action,and the toxicity.Based on changes of pharmacodynamic substance base and pharmacological action before and after processing,the action mechanism of processing in changes of Chinese medicinal properties was explained.