1.Recognizing research fronts and trends of synthetic biology
Li SHENG ; Wei LIU ; Yuxia LI ; Tianxi DIAO
Military Medical Sciences 2015;(2):143-146
Objective To identify research fronts of synthetic biology by mapping knowledge domains .Methods The research articles were clustered by co-cited analysis and bibliographic coupling while research domains of synthetic biology were mapped using software VOSviewer .Results There were seven research clusters by co-cited analysis and 32 clusters by bibliographic coupling .The articles published in the last two years have changed the structure of research domains .Con-clusion Bibliographic coupling is better at recognizing research fronts .Synthetic bioengineering , RNA expression , biology bone, biology library,and biology parts are research fronts of synthetic biology .
2.Metrology and visualized analysis of H5N1 virus:a research of literature
Ting CHEN ; Shuxia WU ; Li SHENG ; Wei LIU ; Tianxi DIAO
Military Medical Sciences 2015;(4):296-301
Objective To analyze domestic and foreign papers on H5N1 virus research using literature metrology and visualized knowledge mapping.Methods Pajek, Bibexcel,and VoSviewer software were used based on Web of Knowledge platform.Results Study on H5N1 virus has been a research focus wide since 2005 and a series of institutions and investi-gators have appeared, with US being the leader in this area and followed by China.The most popular topics of studies on H5N1 included its virology, epidemiology, basic research, anti-virus drugs and vaccines.Conclusion Studies on H5N1 have received more attention, which suggests that more efforts have to be made in China in terms of communication and co-operation with key institutions and famous researchers so as to achieve more success in key areas.
3.Characteristics of U.S.Military Nuclear Medical Emergency Rescue Forces
Tianxi DIAO ; Peng LI ; Lijuan LI ; Wei ZHOU ; Bo FU
Military Medical Sciences 2013;(11):847-849,853
Nuclear medical emergency rescue , as an important task for the military , has become a priority in many countries.The U.S.military has accumulated rich experience in the development of nuclear medical emergency rescue forces.This paper analyzes the legal status , roles, responsibilities and tasks of the U .S.military during nuclear emergency rescue as well as the characteristics and current status of nuclear emergency rescue forces , hoping to provide some refer-ences for development of our military nuclear medical emergency rescue forces .
4.R & D of medicine and vaccine for Ebola hemorrhagic fever
Tianxi DIAO ; Shoujun XU ; Xiaoyu ZHAO ; Wei ZHOU ; Peng LI ; Shu LIU ; Wei WANG
Military Medical Sciences 2014;(8):569-575
The outbreak of Ebola hemorrhagic fever in West Africa in February 2014 is the worst one in the history of the disease.Medicines and vaccines for Ebola have attracted special attention .This study utilizes Cortellis database of Thom-son Reuters and knowledge about Ebola virus to analyze current R&D of medicines and vaccines for Ebola in order to offer references for research and development .Study findings shows that there are mainly 5 important categories:vaccines, neu-tralizing antibodies , small-molecule anti-Ebola drugs , RNAi drugs and nucleotide drugs .In each category , there are prom-ising products under development that are worthy of attention .
5.Establishment of a quality evaluation method for Angelica different processed products from genuine producing areas based on data mining.
Yansheng GUO ; Yongli HUA ; Tianxi DU ; Hongshen YANG ; Yaling QU ; Yanming WEI
Acta Pharmaceutica Sinica 2010;45(9):1155-9
The paper reports the development of a quality evaluation method for Angelica different processed products. The data of high-performance liquid chromatography, water, total ash and extract were analyzed with SPSS Clementine 11.0 software. Discriminant analysis (DA) established the classification model and parameter for Angelica different processed products. Fish's discriminant functions of Angelica different processed products were generated using 8 predictor variables selected from 59 indexes. The correct rate of discriminating back substitution is 96.7%. Angelica different processed products can be accurately and reliably recognized and validated with DA of SPSS Clementine 11.0 software.
6.The Microtox-Based Fast Test of Comprehensive Toxicity of Safflower Injection
Limin LUO ; Liangchun YAH ; Tianxi WEI ; Hua HUA ; Yongjun ZHU ; Junning ZHAO
World Science and Technology-Modernization of Traditional Chinese Medicine 2016;18(11):1935-1941
This study aimed at exploring a new method--microtox technology to evaluate the comprehensive toxicity of safflower injections from different drug manufacturers.In the experiment,we investigated the characteristic parameters of vibrio fischeri (CS234) under different conditions,and then locked the optimum parameters for safflower injection assay:recovery liquid volume 0.9 mL in freeze-dried vial,50 μL bacteria suspension in each sample,and the detection time of 10 mins after adding the bacteria suspension.Under this optimum detection condition,we found that the EC50 values were 3.36%,5.58% and 4.33%,being significantly different between 3 representative drug manufacturers (P < 0.05).In conclusion,it was implied that the biological detection standards of safflower injections need improving,while the microtox-based fast test of the comprehensive toxicity of safflower injection showed a favorable and prospective application to controlling the product qualities of different manufacturers.
7.Organization and implementation of aeromedical evacuation of US patients with Ebola virus infection from West Africa
Peng LI ; Xiaoyu ZHAO ; Wei WANG ; Zhen HE ; Tianxi DIAO ; Shu LIU
Military Medical Sciences 2014;(8):576-578
In 2014,serious Ebola fever epidemic broke out in West Africa .Afterwards, the US transported two Ebola patients from Liberia by air, which aroused world wide concern .During the aeromedical evacuation , there were lots of tech-nical problems and management procedures involved .First, the US government toke emergency measures for the patients in a local hospital ,which made evacuation possible .Secondly , the US CDC and other government departments coordinated this matter during and after the evacuation .Finally,upon arrival at home the two patients received careful isolation and treat-ment.Thus, the successful aeromedical evacuation is instructive for us .
8.Metrology and visualized analysis of anthrax research literature
Wei LIU ; Shuxia WU ; Li SHENG ; Ting CHEN ; Xiaojing ZHANG ; Tianxi DIAO
Military Medical Sciences 2015;(4):292-295,318
Objective To explore the development of the anthrax research field in order to provide information and strategies for anti-bioterrorism in China.Methods Pajek, Vosviewer, Bibexcel running on Web of Knowledge platform, and Excel running on CSCD platform were used.Results According to the results of anthrax literature metrology,the USA is by far the leader in research while the Academy of Military Medical Sciences, Chinese Academy of Sciences and other in-stitutions are our main anthrax research institutions in China, but there is a gap between China and international research institutions.Conclusion We should strengthen anthrax research, improve the overall research strength, and provide the necessary protection to respond to potential bioterrorism incidents.
9.Quality control and discrimination of angelica different processed products based on HPLC fingerprints combined chemometrics methods.
Yansheng GUO ; Yongli HUA ; Tianxi DU ; Hongshen YANG ; Yanming WEI
China Journal of Chinese Materia Medica 2010;35(12):1551-1555
OBJECTIVETo establish a chemical fingerprint method for reorganizing and validating angelica different processed products.
METHODA high-performance liquid chromatographic method was developed to establish the fingerprint. Principal component analysis, hierarchical cluster analysis and discriminate analysis were applied to study HPLC finger printing and chemical pattern reorganization.
RESULTThere were difference of characteristic peaks and its relative peak area of HPLC fingerprints between different processed products. Fish's discriminate functions were generated by using six selected predictor variables, the tested samples of different processed products were classified with 100% accuracy, and discriminate analysis plots for the five groups were well-resolved.
CONCLUSIONThe developed HPLC finger print, combined with chemometrics, can accurately identify and validate angelica different processed products, the research provide theoretical basis for the processing mechanism and quality assess of angelica different processed products.
Angelica ; chemistry ; classification ; China ; Chromatography, High Pressure Liquid ; methods ; Drugs, Chinese Herbal ; analysis ; standards ; Food Handling ; Plant Roots ; chemistry ; Quality Control
10.Sedation and analgesia effects of dexmedetomidine on multiple trauma patients with mechanical ventilation
Anping LIU ; Wei NI ; Peng YE ; Qian YANG ; Tianxi ZHANG ; Anyong YU
Chinese Journal of Trauma 2017;33(12):1118-1122
Objective To explore the sedation and analgesia effect of dexmedetomidine (DEX) in patients with multiple trauma during mechanical ventilation.Methods Eighty cases of multiple trauma patients under mechanical ventilation treated from September 2016 to March 2017 were analyzed by retrospective case-control study.There were 58 males and 22 females with an age range of 18-60 years (mean,41.87 years).The injury severity score (ISS) was (18.45 ±4.53)points.The patients were treated with sedation and analgesia,and they were divided into two groups according to the sedative drugs.DEX composite tartaric acid butorphanol were used in 40 patients as DEX group.Midazolam composite tartaric acid butorphanol were used in 40 patients as Midazolam group.The degree of sedation score (Ramsay score) were compared between groups.The time from initial drug use to effective sedation achievement,daily wake-up time,mechanical ventilation duration,emergency intensive care unit (EICU) time,dosage of tartaric acid butorphanol,heart rate,blood pressure changes,and incidence of delirium were compared.Results (1) Two groups could both reach the target of sedation.DEX group had shorter daily wake-up time,shorter mechanical ventilation time and shorter length of EICU stay compared with Midazolam group (P < 0.05).(2) The time to achieve satisfied sedation after initial usage in Midazolam group was shorter than that in DEX group (P < 0.05).(3) DEX group had smaller tartaric dosage of acid butorphanol,and lower incidence of delirium compared those in Midazolam group (P < 0.05).(4) The comparison of systolic blood pressure,diastolic blood pressure,heart rate within either group showed significant difference before and after sedation (P < 0.05),but had no significant difference between two groups (P > 0.05).Conclusions For multiple trauma patients with mechanical ventilation,DEX can attain sedation and analgesia and shorten daily wake-up time,mechanical ventilation time,and length of hospital stay.DEX can reduce the dosage of analgesic (butorphanol) and the incidence of delirium.Blood pressure and heart rate are associated with small variations before and after sedation.