1.The significance of congenital small nevus in the pathogenesis of malignant melanoma
Tianwen GAO ; Rongqing LIU ; Qingyi YE
Journal of Third Military Medical University 1988;0(05):-
There are controversies concerning whether congential small nevus(CSN)is liable to undergo malignant degeneration and whether it should be resected promptly.The signif-icane of CSN in the pathogenesis of malignant melanoma(MM)was assessed with microspec-trophotometric determination of DNA quantity,the detection of the gene product of N-ras,p21 protein,with ABC technique,ultrastructural study of MM tissue with electron microscopy,and analysis of clinical data of MM.The findings were as follows:(1)The DNA content increased sequentially in order of acquired nevus(AN),CSN,congenital giant nevus(CGN)and MM,and the difference was statistically significant among the 4 groups(P
2.Notch and metal handle broken in artificial hip joint
Changxing LIU ; Wanlin GUO ; Tianwen LI
Chinese Journal of Tissue Engineering Research 2009;13(48):9523-9525
A broken metal handle accompanied by femoral trunk fracture, which would shorten service life of handles. Therefore, it is necessary to judge whether the notch is the main reason for handle broken. Some literatures thought that the pores of sintered porous coated anatomic (PCA) porous layer produce the notch on the handle surface and the notches lead to the handle broken being incorrect. The Apex of the pore is taken for the bottom of the notch, expanding into a crack, and it should also not grow to the base-alloy, accordingly, the above conclusion is wrong. Some reports pointed out the notch, apex of the pore, will expand into the crack, but it is not associated with the broken handle. Under the sintering temperature, the grain boundary apertures be generated on the base metal surface, the aperture is expanded into the crack, which leads to handle broken in the forced area. However, neither theoretical basis, nor a case of corroboration, can prove exist of notch. Actually, the broken handle is caused by the sintering technology. The high temperature of sintering joining results in grain boundary aperture, when encounter the largest forced area and expand into crack, it will break the handle.
3.Influence and effect of joining techniques on porous layer quality of porous coated anatomic
Changxing LIU ; Wanlin GUO ; Tianwen LI
Chinese Journal of Tissue Engineering Research 2009;13(51):10141-10144
INTRODUCTION: General joining techniques are at low level, which produces some potential problems in porous layer of porous coated anatomic (PCA).LIMITATIONS: The problems in general joining techniques are caused by limitations of each technique, which could not be solved using advanced measures.APPLICATION: The activated diffusion brazing technology is an effective way. Its many advanced processes can solve those potential problems.CONCLUSION: The stress-shielding problem caused by PCA porous layer can be solved by using small metal balls in the same diameter in combination with the advanced activated diffusion brazing technology. Moreover, the high performance brazing metals by activated diffusion brazing technology is harmless for humans.
4.Misunderstanding of porous coated anatomic porous layer bonded by sintering technology
Changxing LIU ; Wanlin GUO ; Tianwen LI
Chinese Journal of Tissue Engineering Research 2009;13(47):9337-9340
OBJECTIVE: Sintering of porous coated anatomic porous layer bonding technology remains controversial because of poor understanding to its essence.ESSENCE: Sintering is a technology that the bonding temperature exceeds 5 ℃ or higher than the base-alloy solids temperature of 1 230 ℃, resulting in base-alloy produce a series of serious problems, such as grain boundary racks, handle broken and so on.HAZARDS: In sintering temperature, the low melting point eutectlc phase and the brittle metal compounds flow out of the grain boundary to bond the small metal balls and the handle, which greatly reduces the fatigue strength. The grain boundary cracks formed and expanded ultimately leading to handle broken.DISCUSSION: The cause of a handle broken is neither notch nor metallurgical defects but sintering process, because high sintering temperature results in grain-boundary lacunas.CONCLUSION: Sintering is not suitable for connecting the force components. High sintering temperature produces grain-boundary lacunas which can expand into cracks and ultimately result in handle broken under some condition.
5.Significance of aberrant melanosomes in the diagnosis of malignant melanoma
Tianwen GAO ; Qingyi YE ; Rongqing LIU
Journal of Third Military Medical University 1983;0(04):-
Seventeen cases of malignant melanoma (MM) were observed with transmission electron microscopy in order to clarify the significance of abberant melanosomes in its diagnosis.30 cases of nevus were also studied to serve as control.It was found that aberrant melanosomes were present in 14 cases of MM including 2 cases of amelanotic MM and absent in 2 cases of pigmented MM and one case of amelanotic MM.In addition,aberrant melanosomes were found in 4 cases of congenital nevus.The findings suggest that the significance of aberrant melanosomes in the diagnosis of MM has been overemphasized.It is believed that the diagnosis of MM must be judged comprehensively on all the ultrastructural changes under transmission electron microscope.
6.A study on the labeling of melanocytic tumors and nevi with anti-human proliferative cell antibody Ki-67
Tianwen GAN ; Rongqing LIU ; Qingyi YE
Journal of Third Military Medical University 1988;0(05):-
Fifteen cases of malignant melanoma,17 cases of acquired nevus,18 cases of congenital small nevus,and 3 cases of congenital giant nevus were labeled with anti-human prolifera-tive cell antibody Ki-67 with ABC labeling method.It was found that 13 out of the 15 cases of malignant melanoma were positive and the positive rate of the tumor cells was ranged between 2% to 33%.After culture,the positive rate of the melanoma cells was 88%.But all the 3.types of melanocytic nevi were negative.The findings suggest that the labeling of anti-human proliferative cell antibody Ki-67 is a sensitive and specific method to identify melanocytic tumors and it is not sensitive enough to differentiate the minor changes of the 3 types of nevi.
7.Expression of mouse acidic mammalian chitinase gene, and hydrolysis of fungal cell wall by the en- zyme
Ling CHEN ; Zhu SHEN ; Yufeng LIU ; Bin LIU ; Tianwen GAO
Chinese Journal of Dermatology 1995;0(04):-
Objective To clone and express the mouse acidic mammalian chitinase ( AMCase ) gene and protein, and to analyze the hydrolysis of the cell walls of Trichophyton rubrum and Candida a/ft/cans by this enzyme. Methods Total RNA was isolated from the stomach of BALB/c mouse, and AMCase gene was amplified by RT-PCR. The recombinant fusion expression vector of pET28a ( + ) -AMCase was constructed. AMCase protein was expressed in prokaryotic system and purified. After incubation of AMCase with the cell wall extracts, the level of N- acetylglucosamine was measured. Results AMCase gene was cloned and expressed successfully. Purified AMCase protein can hydrolyse the chitin in the cell walls of T. rubrum and C. albicans. Conclusion The AMCase expressed in prokaryotic system can hydrolyse chitin in the cell walls of T. rubrum and C. albicans, which implies its antifungal potential.
8.The Inhibition of Antisense TRPl in the Proliferation of Malignant Melanoma Cells in vivo and in vitro
Chunying LI ; Tianwen GAO ; Xianlong QI ; Gang WANG ; Yufeng LIU
Chinese Journal of Cancer Biotherapy 1996;0(04):-
Objective: To study the inhibition of antisense TRP1 on cell growth of malignant melanoma(MM) and explore a new way for therapy of melanoma. Methods: Antisense TRP-1 recombinant vector was constructed and transfected into MM cells. According to the results of MTT, cell growth curves were drawn and then clonogenic assay was performed in vitro. At last, tumorigenesis assay was undertaken in nude mice in vivo. Results: Cell proliferations of TRP-1 transfected MM cells were inhibited compared with the control cells. The results of clonogenic assay displayed the difference of clonogenic percentage between TRP-1 transfected MM cells (52% , P
9.Epidemiological investigation on impaired fasting glucose of office worker and enterprise personnel in Shijiazhuang region
Li ZHANG ; Wenzhi WEI ; Tianwen HOU ; Xiaoming LIU
International Journal of Laboratory Medicine 2015;(19):2821-2823
Objective To investigate impaired fasting glucose(IFG)status of office worker and enterprise personnel in Shiji-azhuang area,for providing a basis for early prevention of diabetes mellitus.Methods The fasting blood glucose level of 109 083 of-fice workers and enterprise personnel in Shijiazhuang area were detected in physical examination in 2012.Results The people of IFG (fasting blood glucose 5.6-<7.0 mmol/L)accounted for 14.61% in all subjects,1 6.61% in men,10.23% in women.3.5 1%male of the 18-<21 years old group were IFG.With the increasing of age,the ratio of IFG people gradually rised to a peak of 30.44% at the age group of 71-<76,and then decreased a little.While the proportion of female with IFG was 1.55% at 18-<21 years old group,with the increasing of age,to the age group of 5 1 - < 56 reached the first peak 20.27%,then decreased at the 56-<61 age group.Rapidly rising again,up to 31.74% the second peak in 76-<81 age group,and then decreased a little.Conclu-sion The proportion of IFG in male is higher than that in female.The proportion of IFG increases with age,and reaches the peak in a certain age (over 70 years old),and then at a relatively stable level.
10.Downregulation of K14 Genes and Protein Expression by Liposome Conjugated Antisense Oligonucleotides in Keratinocytes
Yuxin CHEN ; Wei LI ; Jianyong FAN ; Yufeng LIU ; Tianwen GAO
Chinese Journal of Dermatology 1995;0(04):-
Objective To probe into the gene therapy of psoriasis using antisense oligonucleotides to attenuate the expression of K14 gene and protein in keratinocytes and evaluate the inhibitory effects of liposome conjugated antisense oligonucleotides on the proliferation of keratinocytes. Methods The antisense, sense and mismatched oligonucleotides for K14 gene were synthesized and conjugated with lipofectin respectively. Finally they were subsequently transfected into cultured keratinocytes in vitro. The expression of K14 gene was tested by reverse transcription polymerase chain reaction (RT-PCR). The expression of K14 protein was measured by immunohistochemistry. The variation of cell growth cycle was detected by flow cytometry. Results The expression of K14 gene and protein was markedly decreased in keratinocytes treated with K14 antisense oligonucleotides. The cell growth cycle was inhibited effectively by antisense oligonucleotides with lipofection, but not by sense and mismatched oligonucleotides. Conclusions Antisense oligonucleotides conjugated with lipofectin might be a hopeful method to inhibit the proliferation of keratinocytes by inhibiting the expression of K14 mRNA and protein.