Objective To investigate the effect of microcolumn gel reagent card,micro-column glass bead reagent card and classical test tube on the antibody titer of O-type pregnant women with ABO blood group.Determination of antibody potency of pregnant women with rabbit by reagent card.Methods Using microcolumn gel reagent card,micro-column glass beads reagent card,and classical test tube parallel detection to detect the IgG blood group antibody titer of O blood type pregnant women.Results There was no significant difference in the positive detection rate between the microcolumn gel reagent card and the microcolumn glass beads reagent card at 1:128 and the test tube method at 1:64 (P＞0.05).Conclusion The clinical reference value of microcolumn gel reagent card and microcolumn glass beads reagent kit for detecting antibody titer of IgG blood group in O-type pregnant women should be set to ≥ 1:128.

Objective To construct the rapid training program for auxiliary rescue personnel within the restricted area after major disasters,and to provide references for disaster rescue in China.Methods Based on literature review and group discussion,two rounds of consultation with experts using Delphi method were conducted to construct the rapid training program for post-disaster auxiliary rescue personnel.Results The authority coefficients of two rounds of consultations were 0.81 and 0.82,and the coordination coefficients ranged from 0.272 to 0.460.Conclusion Experts had consistent opinions on items of each level for the rapid training program for post-disaster auxiliary rescue personnel.Experts had high levels of enthusiasm and authority,and the rapid training program can meet training requirements for post-disaster auxiliary rescue personnel,and provide references for efficient rescue after major disasters in China.

【Objective】 To explore the clinical applications of low ionic strength salt solution polyethylene glycol (LISS-PEG) and low ionic strength salt solution bovine serum albumin (LISS-BSA) in the indirect antiglobulin test (IAT). 【Methods】 The common standard red blood cell(RBC) IgG irregular antibodies (anti-D, anti-M, anti-N, anti-S, anti-s, anti-Jk^a, anti-Jk^b, anti-Fy^a, anti-Fy^b, anti-Di^a, anti-K) were reacted with RBCs with corresponding antigens in IAT with LISS-PEG and LISS-BSA modification for 5-minute and 10-minute incubations, and then compared the results with conventional IAT. One hundred of blood samples from patients presenting irregular antibodies of erythrocyte IgG were selected to observe the effect of these two self-made modification. The agglutination intensity was recorded by AABB scoring method. 【Results】 No difference was noticed in IAT intensity reaction between LISS-PEG 5-minute and 15-minute incubation (P>0.05), nor between 5-minute/15-minute LISS-BSA incubation and conventional IAT (P>0.05). However, LISS-PEG modification demonstrated a significant superiority over the conventional technique just after incubation with 5 minutes(P<0.05). 【Conclusion】 Using the self-made LISS-PEG as the enhancement medium allows not only reduced incubation time (5 minutes) but also increased intensity of the reaction, which shortens the cross-matching time for emergency blood transfusion and is worthy of popularization.

Osteoarthritis (OA), in which M1 macrophage polarization in the synovium exacerbates disease progression, is a major cause of cartilage degeneration and functional disabilities. Therapeutic strategies of OA designed to interfere with the polarization of macrophages have rarely been reported. Here, we report that SHP099, as an allosteric inhibitor of src-homology 2-containing protein tyrosine phosphatase 2 (SHP2), attenuated osteoarthritis progression by inhibiting M1 macrophage polarization. We demonstrated that M1 macrophage polarization was accompanied by the overexpression of SHP2 in the synovial tissues of OA patients and OA model mice. Compared to wild-type (WT) mice, myeloid lineage conditional Shp2 knockout (cKO) mice showed decreased M1 macrophage polarization and attenuated severity of synovitis, an elevated expression of cartilage phenotype protein collagen II (COL2), and a decreased expression of cartilage degradation markers collagen X (COL10) and matrix metalloproteinase 3 (MMP3) in OA cartilage. Further mechanistic analysis showed thatSHP099 inhibited lipopolysaccharide (LPS)-induced Toll-like receptor (TLR) signaling mediated by nuclear factor kappa B (NF-κB) and PI3K-AKT signaling. Moreover, intra-articular injection of SHP099 also significantly attenuated OA progression, including joint synovitis and cartilage damage. These results indicated that allosteric inhibition of SHP2 might be a promising therapeutic strategy for the treatment of OA.