ObjectiveTo explore the influence of peritoneal transport characteristics on the nutritional status of patients with continuous ambulatory peritoneal dialysis(CAPD).MethodsSeventy-three CAPD patients were involved in this cross-sectional study.According to the results of peritoneal equilibration test(PET),the patients were divided into high transport group with 43 cases and low transport group with 30 cases.Nutritional status was evaluated with the subjective global assessment (SGA) score and the differences were analyzed between two groups in the albumin (ALB),total protein (TP),hemoglobin (Hb),primary disease,age,weight,dialysis time,ultrafiltration,blood urea nitrogen ( BUN ),serum creatinine (SCr) and so on.Results There was no difference between two groups in age,weight,ultrafiltration,systolic blood pressure,diastolic blood pressure,Hb,SGA score,BUN and SCr (P＞0.05).However,the dialysis time,the case number of diabetic nephropathy (DN) as original disease,the case number of non DN as original disease,TP and ALB between high transport group and low transport group had significant differences [(14.35±13.88) months vs.(24.20 ±19.62) months,16 cases vs.12 cases,27 cases vs.18 cases,( 64.98±7.59 ) g/L vs.( 68.73 ± 6.96 ) g/L,( 34.61 ± 5.43) g/L vs.( 38.71±3.82 ) g/L,P ＜ 0.05 ].Conclusions The nutritional status of CAPD patients with different transport characteristics have significant differences.CAPD patients with high transport characteristics are complicated with worse nutritional status,compared with those patients with low transport characteristics.

Objective To investigate the effect of Qi-boosting & Yin-nourishing formula and purslane herb on expression of nitric oxide synthase (NOS) positive neuron in hippocampal CA1 area of diabetic rats. Methods The diabetic rat models were induced by intraperitoneal injection of streptozotocin. After prevented and treated with Qi-boosting & Yin-nourishing formula and purslane herb one month, the diabetic rats were killed, the expression of NOS was detected by NADPH-diaphorase histochemistry. Results The expression of NOS positive neuron in hippocampal CA1 area of diabetic rats which were prevented and treated with Qi-boosting & Yin-nourishing formula and purslane herb was increased (P

Objective To evaluate the influence of peritoneal transport characteristics on nutritional status of peritoneal dialysis patients and estimate the role of peritoneal transport characteristics and nutritional status in evaluation of peritoneal dialysis adequacy. Methods Peritoneal transport properties (D/Pcr) were evaluated by Short Peritoneal Equilibration Test and then 53 cases were divided into high transport group (D/Pcr ≥0. 65) and low transport group (D/Pcr < 0. 65). Urea kinetics (Kt/v) and total clearance of creatinine (TCcr) were calcu- lated. Serum albumin (ALB), prealbumin, transferring (TF), hemoglobin (Hb), lean body mass (LBM) and LBM% were examined or calculated. Subjective global assessment (SGA) was used to evaluate the nutritional sta- tus of patients. Results There were 30 cases in high transport group and 23 cases in low transport group. D/Pcr (0. 82±0.15 vs. 0. 55±0. 08, P < 0. 01), TCcr (62. 93%±25. 98% vs. 49. 69%±16. 92% , P < 0. 05) and age were significantly higher in high transport group than those in low transport group, while ALB, TF, Hb, LBM% , and SGA were significantly lower than those in low transport group (P < 0.05). ALB was negatively cor-related with Kt/v (r= -0.2708, P<0. 05). Conclusions High peritoneal transport patients have better solute clearance but worse nourishment status compared with low transport ones. Nutritional status is one of the factors to evaluate dialysis adequacy. Nutrition management and guidance should be strengthened for the continuous ambula- tory peritoneal dialysis patients, especially for the elderly ones.

Objective To detect the expression and correlated significance of inducible nitric oxide synthase and p53,Bax in benign and malignant diseases of gallbladder. Methods In 16 cases of chronic cholecystitis,11cases of chronic cholecystitis with adenomyoma and 24 cases of gallbladder adenocarcinoma,the expression of iNOS,p53 and Bax in gallbladder's wall was detected by SP immunohistochemistry. Results 1^Inducible NOS and Bax expressed in both benign and maligmant diseased gallbladders' wall,compared with benign diseased gallbladders,the expression of iNOS and Bax in adenocarcinoma was decreased(P

Objective To explore the etiology and pathogenesis of Hirschsprung disease(HD) and the role of nitric oxide in pathophysiology of HD. Methods The whole amount preparations of dilative and transitional segments were stained by NADPH\|diaphorase histochemistry mothod,and colonic walls were digested by dispase. Then the NOS positive nervous structure were observed by light and scanning electron microscope. Results In dilative segment, under light microscope, ganglion and neuron were bigger and more darkly stained. Intraganglionic neurons distributed mostly in peripheral area of ganglion and basal part of ganglionic fiber bundles. Under scanning electron microscope, the neurons were denser, more nerve fibers from them and connected with each other in all dimensions. And more transverse connected fibers between neurons which were arranged along muscle fibers. Myenteric plexus even connected with the submucosal plexus by nerve fibers which passed through circular muscle layer. In transitional segment, under light microscope, intraganglionic neurons cytoplasm were lightly stained and also variedly. The ganglion and neurons of the segment were smaller, and the fibers from them were thinner and paler than those of the dilative segment. Under scanning electron microscope, neurons' density was lower, the fiber connection between neurons and muscle fibers/neurons was lesser. In the transitional segment, neurons and nerve fibers were distributed linearly along the longitudinal muscle fibers. Conclusion Our results indicated that an intimate relationship existed between the development of Hirschsprung disease and abnormal distribution and metabolism of NOS positive neuron in colonic wall. [

Objective To detect the expression of inducible nitric oxide synthase (iNOS) and heme oxygenase-1(HO-1) in human colon of irritable bowel syndrome(IBS), aim at finding out the role of gas messenger molecule such as nitric oxide(NO) and carbon monoxide(CO) in the pathogenesis of IBS. Methods The expression of iNOS and HO-1 in human colonic tissue specimen of enteroscope was detected by En vision and SABC immunohistochemistry each in 34 cases of IBS and 16 cases of normal control group. Results The expression of iNOS and HO-1 expressed mainly in the colonic mucous membrane epithelia, and the expression in IBS group was higher than that in normal control group(P