Objective To investigate effect of laparoscope anesthesia inducted by sevoflurane combined with different doses of propofol on vascular endothelial growth factor-C (VEGF-C) and prostaglandin E2(PGE2)in patients with hysteromyoma.Methods 77 cases of patients with uterine fibroids were selected and divided into two groups.40 cases in group A were anesthetized inducted by 2.0 mg/kg propofol combined with sevoflurane, 37cases in group A were anesthesia inducted by 1.0 mg/kg propofol combined with sevoflurane.VEGF-C and PGE2 in serum were compared before and 48h after the operation.Results Compared with preoperation, VEGF-C and PGE2in serum were lower (P<0.05), VAS score was lower (P<0.05);Compared with group B, VEGF-C and PGE2 in serum of the group A were lower (P<0.05), VAS score was higher (P<0.05).Conclusion Different doses of propofol on serum VEGF-C and PGE2 in patients with laparoscopic uterine fibroids have different effects, 1.0 mg/kg has a sedative effect, 2 0 mg/kg with anesthesia induction.

Objective:To explore the significance of the expression of HIF-1α,MVD and EGFR on diagnostic of nasal inverted papilloma and the clinical effect of taxol .Methods:The expression of HIF-1α,MVD and EGFR were detected by immunohistochemistry in nasal inverted papilloma .Results:The expression of HIF-1α,MVD and EGFR were enhanced with the degree of atypical hyperplasia in epithelium.The research also showed that the expression of HIF-1α,MVD and EGFR were increased dramatically in severe atypical hyperplasia with a significant statistical differences ( P<0.01 ) .The expression of EGFR and MVD showed a positive correlation with HIF-1α.The administration of Taxol alleviated the enhanced expression of HIF-1α, MVD and EGFR with a significant statistical difference(P<0.01),but with on statistical difference with moderately severe tissue (P>0.05).Conclusion:HIF-1α,MVD and EGFR are involved in the pathogenesis and deteriorate of nasal inverted papilloma and can be taken as biomarkers for the diagnosis of the dete -rioration of nasal inverted papilloma .

Bovine pericardium is mainly composed of collagen which has many good properties of extracellular matrix. This in vitro study was performed to quantitatively investigate and compare the degradation behavior of the bovine pericardial, which were modified with different methods. The purpose is to find out one method that presents not only the degradation regularity of material but also minimum antigenicity, so that bovine pericardium can be used as a well degradable guide tissue regeneration material. The results of the experiments showed that the bovine pericardium treated with ethanol is more appreciated than that treated with epoxy cross-linking agent or glutaraldehyde. The mensuration of protein and special amino acid may be a useful base of constructing a mathematic model for further researches on the rate of degradation of bovine pericardium.
Animals ; Biodegradation, Environmental ; Bioprosthesis ; Cattle ; Cross-Linking Reagents ; chemistry ; pharmacology ; Hydroxyproline ; analysis ; In Vitro Techniques ; Materials Testing ; Pericardium ; chemistry ; drug effects ; metabolism ; Prosthesis Design ; Proteins ; analysis ; Surface Properties

Objective To study the effective sterilization method of laparoscopic instruments. Methods Using 2 different methods to sterilize the same laparoscopic instruments,and then compared the effects of sterilization. Results The bactericial rate of the 2 methods were both 100%. Conclusion The Huiri sterilizing method only need 40 minutes,which fits the requirement of one by one operation.

ObjectiveTo investigate the single nucleotide polymorphisms (SNPS) in the coding regions of the human ABCA2 gene and to determine the association of some of these SNPs with gallstone disease in a Chinese population. MethodsThe exons and part of the introns of the ABCA2 gene were sequenced using a fluorescent labeling automatic method in 24 patients with gallstone disease to identify and characterize the SNPs in a Chinese population. For SNPs in the exons, case-control studies were performed on patients and controls. ResultsTwelve SNPs were found within a 16911 bp region of the ABCA2 gene. Among them, two were in the exons, ten in the introns and five were novel SNPs. There was no significant difference in the SNPs genotype between the patients and the controis. ConclusionsThere is an important ethnic difference in the SNPs distribution of the human ABCA2 gene. The distribution of SNPs in the coding regions of the human ABCA2 gene is not significantly different between the patients and the controls.