1.The analysis of the sacroiliac joint CT examination in the detection rate and missed diagnosis of sacral canal cyst
Yanling HUANG ; Cong MENG ; Tianqi ZHAO ; Songbai LI ; Ke XU
Chinese Journal of Postgraduates of Medicine 2014;37(11):14-16
Objective To investigate the detection rate and missed diagnosis reason of sacral canal cyst with sacroiliac joint CT scan.Methods Retrospectively analyzed the changes of sacral canal in 1 286 cases with sacroiliac joint CT scan.CT scanning included bone algorithm reconstruction and standard algorithm reconstruction.Results Among 1 025 patients who had negative sacroiliac joint CT scan were found in 36 cases of sacral canal cyst,the detection rate was 3.5% (36/1 025).Single cyst in 34 cases and multiple cysts in 2 cases.Cyst in S1 level 5 cases,S1-2 level 4 cases,S2 level 22 cases,S2-3 level 4 cases,S3 level 3 cases.The minimal short diameter of the cysts was 0.5 cm and the maximal diameter was 3.2 cm,average 1.2 cm.CT scan showed a round uniform cystic low density,and combined with sacral canal expansion in 19 cases.CT scan used bone algorithm reconstruction in 29 cases,used standard and bone algorithm reconstruction in 7 cases.Clinical findings and correctly diagnosed in 11 cases,accounted for 30.6% (11/36),about 69.4% (25/36) cases were missed diagnosis.Conclusions About 3.5% patients are found sacral canal cyst who have negative sacroiliac joint CT scan.Both the standard and bone algorithm reconstruction should be used to avoid the missed diagnosis of sacral canal cyst.
2.Research on Composite Enzyme Assisted Hyperoside Extraction ofAcanthopanax senticosusand Its Process
Qiulian CHEN ; Manling ZHENG ; Enbo CAI ; Yan ZHAO ; Tianqi ZHAO ; Shumei TU ; Limin YANG
World Science and Technology-Modernization of Traditional Chinese Medicine 2015;(9):1866-1871
This study was aimed to optimize the extraction process of hyperoside from leaves ofAcanthopanax senticosusby compounding-enzyme method and orthogonal experiment. The hyperoside compound was regarded as standard and determined by HPLC. Based on the experiments of 4 factors including the enzyme amount, temperature, extraction time and PH values, the extraction process of hyperoside was determined by the orthogonal experiments and variance analysis. The results of single-factor experiments showed that different enzymes showed different effects on the enhance yield of hyperoside. The effects of different factors showed that the order of PH, neutral protease, temperature, time, pectinase, xylanase and cellulose was from strong to weak. Through orthogonal analysis, the optimum conditions were 2% pectinase, 2% xylanase, 0.5% neutral protease, and 0.5% cellulose, under the temperature of 30°C, extraction time of 10 min, and pH = 4.5. Under these conditions, the extraction rate was 1.84%. The yield was increased 107% compared with traditional process. It was concluded that the use of compounding enzyme can increase the yield of hyperoside, which possessed a lot of economic benefits.
3.Effect of Zinc Doped Calcium Phosphate Coating on Bone Formation and the Underlying Biological Mechanism.
Wenjing LUO ; Jinghui ZHAO ; Xing MENG ; Shanshan MA ; Qianyue SUN ; Tianqi GUO ; Yufeng WANG ; Yanmin ZHOU
Journal of Biomedical Engineering 2015;32(6):1359-1363
Implant surface modified coating can improve its osteoinductivity, about which simple calcium phosphate coating has been extensively studied. But it has slow osteointegration speed and poor antibacterial property, while other metal ions added, such as nano zinc ion, can compensate for these deficiencies. This paper describes the incorporation form, the effect on physical and chemical properties of the material and the antibacterial property of nano zinc, and summarizes the material's biological property given by calcium ion, zinc ion and inorganic phosphate (Pi), mainly focusing on the influence of these three inorganic ions on osteoblast proliferation, differentiation, protein synthesis and matrix mineralization in order to present the positive function of zinc doped calcium phosphate in the field of bone formation.
Biocompatible Materials
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Calcium
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Calcium Phosphates
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chemistry
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Cell Differentiation
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Cell Proliferation
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Humans
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Ions
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Metal Nanoparticles
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chemistry
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Osteoblasts
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cytology
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Osteogenesis
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Phosphates
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chemistry
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Zinc
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chemistry
4.The effect of shoulder subluxation on the electrophysiological characteristics of the peripheral nerves in the upper limbs of stroke survivors: A self-controlled study
Xiangzhe LI ; Panpan XU ; Sheng WANG ; Xiaomeng ZHAO ; Tianqi WEI ; Xifeng LI ; Na MEI ; Wei ZHOU ; Qinfeng WU
Chinese Journal of Physical Medicine and Rehabilitation 2021;43(2):122-127
Objective:To explore the effect of shoulder subluxation on the peripheral nerves in the hemiplegic upper limbs of stroke survivors.Methods:Twenty stroke survivors with shoulder subluxation were enrolled. Conduction in their suprascapular, axillary, musculocutaneous, radial, median and ulnar nerves was monitored and needle electromyography was used to monitor activity in the supraspinatus, deltoid, biceps brachii, extensor digitorum, abductor pollicis brevis and abductor digiti minimi muscles of their affected upper limbs at rest. Upper limb and hand function were assessed using the Brunnstrom scale. The rate of change in the amplitude of the compound muscle action potentials (CMAPs) was correlated with the patient′s disease duration, age, and upper limb and hand Brunnstrom stages.Results:Compared with the healthy side, a significant decrease was observed in the CMAP amplitudes of the suprascapular, axillary, musculocutaneous, radial, median and ulnar nerves of the hemiplegic arm, and the latency of the suprascapular and axillary nerves was significantly prolonged. There was no inter-arm difference in the conduction velocity of the musculocutaneous, radial, median and ulnar nerves. The rates of change in the CMAP amplitudes of the suprascapular, axillary and musculocutaneous nerves were significantly higher than those of the radial, median and ulnar nerves. The sensory nerve action potential (SNAP) amplitudes of the median, ulnar and radial nerves on the hemiplegic side were significantly lower than on the healthy side, but there was no significant difference in the sensory conduction velocity between the two sides. On the hemiplegic side, the median nerve had the highest rate of change rate in the SNAP amplitude, followed by the radial and ulnar nerves, but there was no significant difference among them. Nor was there any significant difference in the rate of change in sensory nerve conduction velocity. The muscles of the affected upper limbs had higher potentials in the proximal than that in the distal nerves after shoulder subluxation. The rate of change in the CMAPs was not significantly correlated with a patient′s disease duration, age, or upper limb or hand Brunnstrom stage on the hemiplegic side.Conclusions:Shoulder subluxation after a stroke can cause greater damage to the peripheral nerves in the shoulder and upper arm than to those in the forearm and hand, possibly affecting the recovery of upper limb function.
5.Sinapic Acid Attenuates the Neuroinflammatory Response by Targeting AKT and MAPK in LPS-Activated Microglial Models
Tianqi HUANG ; Dong ZHAO ; Sangbin LEE ; Gyochang KEUM ; Hyun Ok YANG
Biomolecules & Therapeutics 2023;31(3):276-284
Sinapic acid (SA) is a phenolic acid that is widely distributed in fruits and vegetables, which has various bioactivities, such as antidiabetic, anticancer and anti-inflammatory functions. Over-activated microglial is involved in the development progress of neurodegenerative diseases, such as Parkinson’s disease and Alzheimer’s disease. The objective of this study was to investigate the effect of SA in microglia neuroinflammation models. Our results demonstrated that SA inhibited secretion of the nitric oxide (NO) and interleukin (IL)-6, reduced the expression of inducible nitric oxide synthase (iNOS) and enhanced the release of IL-10 in a dose-dependent manner. Besides, our further investigation revealed that SA attenuated the phosphorylation of AKT and MAPK cascades in LPS-induced microglia. Consistently, oral administration of SA in mouse regulated the production of inflammationrelated cytokines and also suppressed the phosphorylation of MAPK cascades and AKT in the mouse cerebral cortex. These results suggested that SA may be a possible therapy candidate for anti-inflammatory activity by targeting the AKT/MAPK signaling pathway.
6.Role of autophagy in orthodontic tooth movement
Tianqi WANG ; Chengcheng LIAO ; Jianguo LIU ; Lulu CHEN ; Piao ZHAO ; Linlin XIAO ; Xiaoyan GUAN
Chinese Journal of Tissue Engineering Research 2024;28(34):5558-5564
BACKGROUND:The application of orthodontic force triggers autophagy in the periodontal tissue via diverse signaling pathways,augmenting or attenuating the activity of relevant cell types such as periodontal ligament cells,osteocytes,osteoclasts,and osteoblasts,thus facilitating the process of periodontal remodeling. OBJECTIVE:To review the research progress in orthodontic force mediated autophagy in periodontal tissue and its impact on orthodontic tooth movement. METHODS:The PubMed,Web of Science,China Biology Medicine disc and CNKI were searched for literature published from 2010 to 2023 to summarize the progress in orthodontics-related autophagy.And 76 papers were finally included in the analysis and discussion. RESULTS AND CONCLUSION:Orthodontic force can trigger a series of biochemical signal changes through periodontal mechanical receptors and aseptic inflammation they cause,leading to autophagy in periodontal tissue.Subsequently,autophagy generates corresponding feedback through cascaded amplified signaling pathways such as Phosphoinositide 3-kinase/protein kinase B,Hippo,and mitogen-activated protein kinase pathways,promoting periodontal tissue remodeling and ultimately achieving tooth movement and stability.Orthodontic force-induced autophagy can differentially regulate bone resorption on the tooth pressure side and bone formation on the tension side.Related targets have good prospects in the clinical application of orthodontic treatment.Orthodontics and autophagy have complex mechanisms.However,existing research has only focused on exploring the role of autophagy in orthodontic tooth movement.Further exploration is needed to investigate the mutual regulatory effects between autophagy and orthodontic tooth movement,as well as the interactions between upstream mechanical receptors and signaling pathways involved in related pathways.
7.Commutability Assessment of Candidate External Quality Assessment Materials for Aminotransferase Activity Measurements Based on Different Approaches in China
Qichen LONG ; Tianqi QI ; Tianjiao ZHANG ; Jing WANG ; Jie ZENG ; Ying YAN ; Meng WANG ; Wei HUANG ; Haijian ZHAO ; Wenxiang CHEN ; Chuanbao ZHANG
Annals of Laboratory Medicine 2021;41(1):68-76
Background:
Using commutable external quality assessment (EQA) materials is important for monitoring successful harmonization efforts. We assessed the commutability of four human serum pool (HSP) preparations to identify candidate EQA materials for alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity measurement.
Methods:
One set each of 85 clinical samples (CSs) was collected for ALT and AST activity measurement. The 15 candidate EQA materials included four types of HSP preparations (A to D): materials A, C, and D contained human original recombinant (HOR) aminotransferases; materials B was mixed leftover samples. The CSs and 15 candidate EQA materials were analyzed using seven routine assays, and the ln-transformed results were analyzed in 21 assay pairs. Commutability was assessed using Deming regression, with a 95% prediction interval (CLSI approach) and the difference in bias with an error component model (International Federation of Clinical Chemistry and Laboratory Medicine [IFCC] approach).
Results:
For ALT, all materials were commutable for 14–21 assay pairs according to the CLSI and IFCC approaches. For AST, B01-03 showed commutability for 14-21 assay pairs, and C01-03 and D01-03 showed commutability for no less than 10 assay pairs according to the two approaches. A01-06 were commutable for 9-16 assay pairs according to the CLSI approach, but for 6-9 assay pairs according to the IFCC approach.
Conclusions
Mixed leftover samples showed desirable commutability characteristics as candidate EQA materials for routine aminotransferase activity measurements. Human serum bases supplemented with HOR were commutable for most routine ALT activity measurements.
8.In vitro study on cyclic fatigue resistance of three types of nickel titanium files in preparation for bending root canals
WANG Yuxin ; JIAO Rentian ; ZHAO Ying ; WANG Tianqi ; LIANG Guangzhi
Journal of Prevention and Treatment for Stomatological Diseases 2024;32(2):101-107
Objective
The purpose of this study was to investigate the differences in the anti cyclic fatigue performance of Woride KS (WKS), Proteper Gold (PTG), and Hyflex CM (HCM) nickel titanium instruments with different tip diameters in curved root canal models, and to provide reference for the targeted selection of suitable nickel titanium instruments in clinical preparation of curved root canals.
9.Experimental study on the treatment of sphincter of Oddi dysfunction by Qingre Lidan Decoction
Yixuan LIANG ; Haonan LIN ; Wangqiang ZHAO ; Junwei CAO ; Tianqi WANG ; Changmiao WANG
Chinese Journal of Hepatobiliary Surgery 2023;29(11):857-861
Objective:To study the changes in the sphincter of Oddi pressure in rabbits after bilateral vagus nerve trunk severance and the therapeutic effect of Qingre Lidan Decoction, to provide a new way for the treatment of sphincter of Oddi dysfunction.Methods:Twenty-four 1.5~2.0 kg New Zealand Large White rabbits of either gender were randomly selected and divided into the control group, the model group, and the treatment group. In the control group, only pyloroplasty was performed; in the model group and the treatment group, pyloroplasty plus bilateral vagus nerve trunk dissection at the level of the diaphragm were performed, and in the treatment group, one month of gavage treatment with Qingre Lidan Decoction was carried out 7 days after the operation. Multi-channel bio-signal acquisition system was used to record the pressure changes of the sphincter of Oddi in rabbits of each group; ELISA was used to detect the changes of inflammatory factors IL-6 and tumor necrosis factor TNF-α in the serum of rabbits.Results:The pressure of the sphincter of Oddi in the model group was significantly higher than those in the control group [low-pressure area: (51.95±0.35) mmHg (1 mmHg=0.133 kPa) vs (21.60±1.13) mmHg ( P<0.05) ; High pressure area: (60.75±0.49) mmHg vs (20.70±0.85) mmHg ( P<0.05)], the pressure of sphincter of Oddi in the treatment group of Qingre Lidan Decoction was lower than that of the model group [low-pressure area: (22.70±1.13) mmHg vs (51.95±0.35) mmHg ( P<0.05); high-pressure area: (32.15±0.49) mmHg vs (60.75±0.49) mmHg ( P<0.05)]. Serum IL-6 and TNF-αwere significantly elevated in the model group compared to the control group; IL-6 and TNF-α levels were decreased in the treatment group compared to the model group, and the differences were statistically significant ( P<0.05). Conclusion:Bilateral vagus nerve trunk severance leads to Oddi sphincter dysfunction, and treatment with Qingre Lidan Decoction could improve Oddi sphincter dysfunction.
10.Baitouweng Tang Suppresses Colorectal Cancer HCT116 Cells by Regulating Hedgehog Signaling Pathway
Maolun LIU ; Shan REN ; Han YANG ; Hui ZHAO ; Qiu TAO ; Shun TANG ; Tianqi MING ; Haibo XU
Chinese Journal of Experimental Traditional Medical Formulae 2023;29(8):125-132
ObjectiveTo explore the effect of Baitouweng Tang (BTWT) on the apoptosis of human colorectal cancer HCT116 cells and decipher the underlying mechanism based on the Hedgehog (Hh) signaling pathway. MethodHCT116 cells were treated with BTWT (25, 50, 100, 200, 500, 750, and 1 000 mg·L-1) for 24 h, and then the cell proliferation was detected by methyl thiazolyl tetrazolium (MTT) colorimetry. Five groups were designed for the treatment of HCT116 cells, including a blank control group, BTWT groups (125, 250, and 500 mg·L-1), and a positive control (5-fluorouracil, 5-FU, 40 mmol·L-1) group. The cell morphology was observed under an inverted microscope. The migration of the cells was detected by scratch test, and the apoptosis by Hoechest 33324/propidium iodide (PI) staining and flow cytometry. Western blot was employed to determine the protein levels of sonic hedgehog (SHh), GLI family zinc finger protein 1 (Gli1), smoothened (Smo), suppressor of fused (SuFu), cellular-myelocytomatosis viral oncogene (c-Myc), and the apoptosis-related proteins B-cell lymphoma-2 (Bcl-2) and Bcl-2-associated X protein (Bax). The quantitative real-time reverse transcription PCR (Real-time PCR) was employed to determine the mRNA levels of Bax, Bcl-2, SHh, Gli1, Smo, SuFu, and c-Myc. ResultCompared with the blank control group, BTWT changed the cell morphology (making the cell become round with dense nucleus), inhibited the proliferation of HCT116 cells in a dose-dependent manner, decreased the ability of migration (P<0.05, P<0.01), and increased apoptotic cells. Compared with the blank control group, BTWT (500 mg·L-1) treatment for 24 h up-regulated the protein and mRNA levels of Bax (P<0.05, P<0.01) and down-regulated the protein and mRNA levels of Bcl-2 in HCT116 cells (P<0.05, P<0.01). Moreover, the treatment down-regulated the mRNA and protein levels of SHh, Gli1, Smo, and c-Myc (P<0.05, P<0.01) and up-regulated the mRNA and protein levels of SuFu (P<0.05, P<0.01). ConclusionBTWT inhibited the proliferation and migration and induced the apoptosis of colorectal cancer HCT116 cells by down-regulating the Hh signaling pathway.