Objective To explore the association between estrogen receptor gene polymorphism and systemic lupus erythematosns in women of Han nationality from Jiangxi province.Methods Whole blood samples were obtained from 95 female patients with SLE of Han nationality (18 with pre-menarche onset and 77 with post-menarche onset) and 100 healthy female controls from Jiangxi province.Genomic DNA was extracted from these samples followed by the analysis of polymorphism at Pvu Ⅱ and Xba I sites of estrogen receptor alpha gene with PCR-restriction fragment length polymorphism (RFLP).The frequencies of alleles and genotypes were compared between the control and patients and between patients with pre-menarche onset and those with post-menarche onset.Results The frequency of P allele was higher in patients with SLE than in the controls (35.8% vs 27%,P<0.05),but lower in patients with pre-menarche onset than in those with post-menarche onset (19.4% vs 39.6%,P<0.05).Further more,an increment was observed in the frequency of Xxpp genotype in patients with pre-menarche onset compared with normal controls and patients with postmenarche onset (16.7% vs 1% and 1.3%,both P<0.05).Conclusions The frequency of P allele in ER alpha gene is increased in female patients with SLE of Han nationality from Jiangxi province.The Xxpp genotype of ER gene may be associated with the susceptibility to pre-menarche onset SLE.

Objective To investigate the efficacy of N-acetylcysteine on prevention of contrast -induced nephropathy (CIN).Methods According to the regulation of evidence-based medicine,the selection criteria,elimination criteria and search strategy were defined.PubMed,Cochrane Library,Wiley Online Library and Google Scholar were searched.The literature limited range was from January 2000 to December 2011.Two investigators extracted the data independently from all the studies that accorded with selection criteria using a suitable form.All the statistical analyses were performed with RevMan version 5.1.Results A total of 151 potential literatures were screened and 16 remained literatures (including 4588 patients) were identified to accord with the criteria in this meta-analysis.In 14 literatures,the Jadad score was 3 at least.The meta-analysis of 16 trials showed N-acetylcysteine could prevent CIN from happening [odds ratio (OR)=0.65,95％ CI 0.46-0.92,P=0.01].In the groups of average Scr baseline ＜ 132.6 μmol/L,result displayed the OR of incidence associated with Nacetylcysteine for prevention of CIN was 0.93 (95％ CI 0.75-1.15,P=0.49).In the groups of average Scr baseline ≥ 132.6 μmol/L,the OR for N-acetylcysteine associated with incidence of CIN was 0.52 (95％ CI 0.30-0.93,P=0.03).Conclusion There is specific effect that N-acetylcysteine prevents CIN from happening in the groups of average Scr baseline ≥ 132.6 μmol/L.

Objective To investigate the infection situation of HIV in patents in hospital ,and to provide basis for the prevention of AIDS in the hospital .Methods Three kinds of enzyme‐linked immunosorbent assay(ELISA)kits(Beijing wantai ,Beijing kewei , French BIO‐RAD)were used for screening HIV antibody and antigen in 240 781 samples from Jan .2011 to Dec .2013 .All HIV re‐peated positive screening samples were confirmed by western blotting in Chongqing shapingba Center for Disease Control and Pre‐vention .Results Among 240 781 samples from 2011 to 2013 ,593 samples(0 .246% ) were HIV positive at the first screening ,558 samples(0 .231% ) were HIV positive in at the repeated screening ,29 samples(0 .012% ) were HIV indeterminate ,6 samples(0 . 002% ) were HIV negative.Male and female ratio was 3 .39:1 .Conclusion Screening in hospital patients could be an important way to discover cases with HIV infection .It is nesscessary to strengthen the promotion and propaganda of HIV detection ,and new technology of HIV detection could be used to strengthen the inspection of HIV .Moreover ,the consciousness of self protection should be promoted in the treatment of HIV patients .

OBJECTIVE: To examine the circulating levels of miR-214 in acute myocardial infarction (AMI) patients and to explore the relationship between the circulating levels of miR-214 and the degree of coronary lesion. METHODS: A total of 45 patients with AMI (AMI group) were enrolled from Xiangya Hospital of Central South University between September, 2011 and January, 2012. Twenty healthy volunteers served as a normal control group (n=20). According to the coronary artery lesion area, AMI group was also divided into a single-branch group, a double-branch group and a triple-branch group (n=20, 13, 12 respectively). Circulating levels of miR-214 and plasma levels of placental growth factor (PLGF) were measured by real time-PCR assay and enzyme-linked immunosorbent assay respectively on the day when the patients admitted to the hospital. The plasma levels of miR-214 and PLGF were compared among the various branch groups. The correlation between miR-214 and PLGF was analyzed in AMI subgroups. RESULTS: The miR-214 levels in the AMI subgroups were lower than that in the control group. The more decrease in miR-214 level, the larger size of coronary lesion. There was significant difference in the different groups (all P<0.05). Compared with the control group, the levels of plasma PLGF were significantly higher in the AMI subgroups. The more increase in PLGF level, the larger size of coronary lesion. There was significant difference in the different groups (all P<0.05). The plasma levels of miR- 214 were negatively correlated with that of PLGF in the AMI group (r=-0.588, P<0.01). CONCLUSION The circulating level of miR-214 was significantly decreased in the AMI group, which might be correlated with the extent of the coronary lesion. Circulating miR-214 may be a promising biomarker for the diagnosis and prognosis of severe AMI.
Biomarkers ; blood ; Case-Control Studies ; Enzyme-Linked Immunosorbent Assay ; Humans ; MicroRNAs ; blood ; Myocardial Infarction ; pathology ; Placenta Growth Factor ; Pregnancy Proteins ; blood ; Prognosis

Obstructive sleep apnea syndrome (OSAS) is a complicated chronic disease caused by certain reasons, characterized by obstruction of the upper airway and apnea or hypopnea during sleep, which can be followed by anoxia, snoring and daytime sleepiness. Recent studies have shown that hypertension is closely connected to OSAS. OSAS can lead to hypertension by several possible mechanisms. The diagnosis of OSAS mainly depends on the medical history, sign, polysomnogram (PSG) result and the frequency of apnea and hypopnea. OSAS can be relieved by continuous positive airway pressure (CPAP), oral orthodontic treatment, medicine, change of lifestyles and others. This brief review focuses on the mechanism of hypertension due to OSAS and the diagnosis criteria and treatment of OSAS.
Continuous Positive Airway Pressure ; Humans ; Hypertension ; epidemiology ; Polysomnography ; Sleep Apnea, Obstructive ; epidemiology ; Snoring