1.Dietary assessment on dietary exposure of aluminium residents in Shaanxi Province
Xiaoling NIE ; Rong GUO ; Yu LIU ; Tianlai LI ; Minjuan WANG
Chinese Journal of Food Hygiene 2017;29(4):499-504
Objective To investigate the contamination and dietary intake of the aluminium in commercial foods in Shaanxi Province,and evaluate its potential health risks in Shaanxi population.Methods Six hundred and sixty-six samples from 8 kinds of foods in Shaanxi Province were collected from 2013 to 2015.The aluminium contents were detected according to National Food Contamination and Harmful Factors in Risk Monitoring Manual,and dietary intake of aluminium in Shaanxi population was evaluated by point assessment model with of nutritional survey date.Results The average dietary intake of aluminium was 0.154 9 mg/kg BW per day,and the dietary intake of woman aged 18 to 59 years old was the highest,with an average of 0.178 5 mg/kg BW per day.When aluminum intake of the 97.5 percentile,the dietary intake of aluminium in children aged 2 to 7 years old was the highest.The margins of safety (MOS) of all age groups were smaller than 1,while the MOS of high exposure (P97.5) group was above 1.Fried and baked food were the main sources of aluminium exposure in two age groups (2-7 years and 8-12 years old group),while fried food,cold noodle and starch products were the main sources of aluminium exposure for others.Conclusion Dietary intake (tP97.5) of aluminium exceeded the provisional tolerable weekly intake (PTWI).The dietary intake of aluminium in women and children was high.Fried food was the main sources of aluminium exposure in all age groups.
2.THE PHARMACOKINETIC CHARACTERISTICS OF BORNEOL IN SERUM AND BRAIN TISSUE OF RATS
Meirong LIANG ; Qide LIU ; Tianlai HUANG ; Yinqing ZHANG ; Weiping OU ;
Traditional Chinese Drug Research & Clinical Pharmacology 1993;0(04):-
The concentrations of borneol in the serum and brain tissue of rats were determined by gas chromatography. Using brain tissue as a relatively independent system, PHARMFIT and PCNONLIN softwares are applied for the kinetic analysis of the infracorporal process of borneol in order to find out and compare the pharmacokinetic characteristics of borneol in the brain and serum. The results showed that borneol was absorbed very rapidly in the gastrointestinal tract and readily permeates the blood-brain barrier into brain tissue. There was a higher specific value of borneol concentration in the brain and serum. Moreover, the half life of distribution phase of borneol in the brain was three times higher than that in the serum. But the constants of elimination rate of borneol in the brain and serum decreased simultaneously and parallelly, hence the accumulation of borneol in brain will not be found.
3.Application of Rituxnab in antibody-mediated rejection among renal transplantation recipients
Yongguang LIU ; Tianlai LIU ; Min LI ; Ying GUO ; Hua CHEN ; Jianmin HU ; Lipei FAN ; Liangsheng YUE ; Liuyang LI ; Ming ZHAO
Chinese Journal of Organ Transplantation 2011;32(9):531-533
Objective Toobservetheindication, safetyandefficacyofanew immunosuppressant Rituximab in kidney transplantation. MethodsFive patients, who were diagnosed as antibody mediated rejection (AMR) from December 2010 to June 2011, were treated with single dose of Rituximab (500 mg) and followed up for 6 months. The clinical data, such as age, gender, onset of illness, induction therapy, maintaining therapy, allograft function, change of PRA, opportunistic infection and other complications were collected and retrospectively analyzed to evaluate the safety and efficacy of Rituximab used in AMR patients. ResultsAfter Rituximab therapy, all the patients had improved renal function measured by sera creatinine level: 4 cases retumed to normal, and 1 keep stable. Series of allograft biopsy demonstrated obviously reduced C4d deposition in nephridial tissue after treatment. One patient developed CMV viremia, another had urinary infection, but no one had lifethreatening infection during the follow-up period. The survival rate of human and allograft was both 100 %. Conclusion Rituximab has a good efficacy and safety in treatment of AMR after renal transplantation.
4.Inducible and constitutive expression of fip-fve from Flammulina velutipes in Pichia pastoris GS115.
Jingwei LIN ; Jia JIA ; Ming ZHONG ; Lijing CHEN ; Haoge LI ; Zhifu GUO ; Mingfang QI ; Lixia LIU ; Tianlai LI
Chinese Journal of Biotechnology 2014;30(3):464-471
We transformed the fip-fve gene into Pichia pastoris GS115 for inducible and constitutive expression to obtain feasible bioactvie recombinant Fip-fve. The fip-fve gene was cloned from Flammulina velutipes fruting body by PCR and ligated to pPIC9 to construct inducible expression vector pPIC9-FIP-fve, and promotor pgap was used to replace the paox1 to construct constitutive expression vector pPIC9-PGAP-FIP-fve. These two vectors were used to transform P. pastoris by PEG method. The fip-fve was expressed after histamine-absence screening and yeast colony PCR. The inducible expression level reached 158.2 mg/L at the fourth day and the constitutive expression level was 46.3 mg/L and 29.5 mg/L using glucose and glycerol, respectively. The SDS-PAGE and Western blotting both proved the correctness of rFip-fve, and the hemagglutination test indicats the rFip-fve's bioactivity.
Electrophoresis, Polyacrylamide Gel
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Flammulina
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chemistry
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Fungal Proteins
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biosynthesis
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Genetic Vectors
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Pichia
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metabolism
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Polymerase Chain Reaction
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Promoter Regions, Genetic
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Recombinant Proteins
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biosynthesis
5.Development of environmentally friendly flexible medical X-ray shielding materials and analysis and optimization of their protective performance
Tianyi QIU ; Guoqing ZHANG ; Minjuan WANG ; Tianlai LI ; Boyu WANG ; Yang LIU ; Boning LI ; Lin YUAN
Chinese Journal of Radiological Medicine and Protection 2023;43(12):1016-1021
Objective:To develope and analyze and optimize the performance of some kinds of environmentally friendly flexible X-ray protective materials in attempt to tackle the various environmental and high energy consumption problems in the development of traditional medical X-ray protective clothing.Methods:The Monte Carlo program was used to establish a simplified model of medical X-ray tube. The aim was to carry out numerical simulation and prediction of the shielding materials′ performance against X-ray, prepare the flexible X-ray shielding materials through experiments and test and verify the their shielding performances The development and optimization path was also obtained by comparing the result between simulation and experiment.Results:Bi was the preferred alternative to toxic Pb elements, while W was able to compensate for weak X-ray absorption zone of Bi. The shielding efficiency of the composite material doped with 25% Bi+ 25% W was able to reach 77.8% and 66.3% at 80 and 120 kV p tube voltages, respectively. Conclusions:With both the selection of elements and the optimization of functional particles, the combination of W and Bi is an economical, environmentally friendly, and efficient shielding way within the energy range of medical diagnostic X-rays. The numerical simulation helps reduce experimental costs, shorten the research period, and improve the design efficiency of X-ray shielding materials.
6.Correlation between work fatigue and work-related musculoskeletal pain or injury in the occupational population in China
Qing XU ; Huadong ZHANG ; Ruijie LING ; Yimin LIU ; Gang LI ; Zaoliang REN ; Yan YIN ; Hua SHAO ; Hengdong ZHANG ; Bing QIU ; Meibian ZHANG ; Dayu WANG ; Qiang ZENG ; Rugang WANG ; Jianchao CHEN ; Danying ZHANG ; Liangying MEI ; Yongquan LIU ; Jixiang LIU ; Chengyun ZHANG ; Tianlai LI ; Ning JIA ; Zhongxu WANG
China Occupational Medicine 2023;50(2):133-139
7.Improvement Effects of Sanggenone C on Lipid Accumulation in FFA-induced Human Liver Cancer HepG 2 Cells
Juling XING ; Fen LIU ; Meng FENG ; Mengjiao HAO ; Tianlai HUANG ; Xinxin ZHOU ; Xiangjiang TANG
China Pharmacy 2021;32(15):1868-1873
OBJECTIVE:To study the improvement ef fects of sanggenone C on lipid accumulation in human liver cancer HepG2 cells induced by free fatty acid (FFA). METHODS :HepG2 cells were divided into control group ,model group , fenofibrate group (10 μmol/L),sangerone C low ,medium and high concentration groups (2,4,8 μmol/L). Except for control group,other groups were treated with 1 mmol/L FFA to induce lipid accumulation model ,and administration groups were cultured with relevant medium containing drugs. The lipid accumulation was observed by oil red O staining ,and lipid level and triglyceride (TG) content were also determined. Real-time PCR and Western blot assay were adopted to detect the mRNA and protein expression of PPARα,CPT-1,SREBP-1c,FAS,SIRT1 and PGC- 1α in HepG2 cells. RESULTS :Compared with control group , the nucleus was atrophied significantly and the volume became smaller ,and the number of lipid droplets was significantly increased;the level of lipid ,TG content ,mRNA and protein expression of SREBP- 1c and FAS were significantly increased (P< 0.05 or P<0.01),mRNA and protein expression of PPARα,CPT-1,SIRT1 and PGC- 1α were decreased significantly(P<0.01). Compared with model group ,no obvious nucleus atrophy and normal volume were observed in sangerone C groups ,and the number of lipid droplets was significantly reduced ;the levels of lipid ,TG content ,mRNA and protein expression of PPARα pathway related genes (except for SREBP- 1c protein in saggenone C low concentration group )were significantly reversed (P< 0.05 or P<0.01). CONCLUSIONS :Sangenone C can significantly improve the lipid accumulation of HepG 2 cells,and its mechanism may associated with regulating PPAR α signaling pathway,improving cell lipid oxidation ability and inhibiting lipid synthesis.
8.Analysis of proficiency test for determination of manganese in drinking water in Shaanxi Province
Yu LIU ; Wenting YUAN ; Caixia WANG ; Guoxia CHENG ; Xiaoling NIE ; Xiaocong LIANG ; Tianlai LI
Journal of Public Health and Preventive Medicine 2021;32(2):26-29
Objective To understand and improve the laboratory detection capacity of water manganese in testing organizations of Shaanxi Province. Methods The self-made manganese capacity testing samples were used as the reference in this proficiency testing program. The homogeneity of the samples was tested by single factor analysis of variance. The t-test method was used to evaluate the stability of the samples. The results of manganese in water provided by participant laboratories were analyzed by the robust statistical technique Z-score. Results A total of 136 laboratories participated in the proficiency testing program throughout the province. Among them, 129 laboratories (including retest laboratory) or 94.85% of total participating laboratories obtained satisfactory results. Conclusion The water quality monitoring agencies of our province had shown a strong detection capability for the determination of metal elements in drinking water, which ensures to provide safe water supply to the residents in Shaanxi Province.
9.Association between wrist pain and awkward postures among workers in 10 key industries
Guanlin LI ; Xin SUN ; Meibian ZHANG ; Huadong ZHANG ; Ruijie LING ; Yimin LIU ; Gang LI ; Nengzhou CHEN ; Zaoliang REN ; Yan YIN ; Hua SHAO ; Hengdong ZHANG ; Jiajie LI ; Bing QIU ; Dayu WANG ; Qiang ZENG ; Zhanhui LIANG ; Rugang WANG ; Jianchao CHEN ; Danying ZHANG ; Liangying MEI ; Yongquan LIU ; Jixiang LIU ; Chengyun ZHANG ; Tianlai LI ; Ning JIA ; Junyi WANG ; Zhongxu WANG ; Qingsong CHEN
Journal of Environmental and Occupational Medicine 2023;40(1):49-54
Background Prolonged awkward postures during occupational activities can lead to excessive musculoskeletal load on the wrist of workers and symptoms such as wrist pain or discomfort. Objective To survey the prevalence of wrist pain among workers in 10 key industries and analyze its correlation with wrist working postures. Methods By using stratified cluster sampling method, workers from 10 key industries, such as footwear manufacturing industry, shipbuilding manufacturing industry, and automobile manufacturing industry, were selected from seven regions in North China, East China, Central China, South China, Southwest China, Northwest China, and Northeast China. The demographic information, wrist working postures, pain in wrist of the workers were collected through a cross-sectional survey. Pearson χ2 test was used to compare prevalence by selected factors, trend χ2 test for between group comparison, and unconditional logistic regression models for the association of wrist working postures with wrist pain. Results There were 64052 workers enrolled in this survey, and 56286 provided valid questionnaires (the effective rate was 87.8%). According to the survey, the prevalence of wrist pain was 23.3% (13112/56286), and the industries with higher prevalences were footwear manufacturing (27.1%, 1927/7106), automobile manufacturing (24.9%, 5378/21560), and shipbuilding and related equipment manufacturing (24.4%, 850/3488) industries. Finger pinching (OR=2.09, 95%CI: 1.95-2.24), frequent wrist bending (OR=2.03, 95%CI: 1.92-2.15), fixed wrist bending (OR=1.77, 95%CI: 1.69-1.85), wrist on hard edge (OR=1.34, 95%CI: 1.28-1.40), and arms over shoulders (OR=1.11, 95%CI: 1.05-1.17) increased the risk of reporting wrist pain. Conclusion Awkward postures are related to wrist pain among workers in selected 10 key industries. The related factors are wrist on hard edge, frequent wrist bending, finger pinching, fixed wrist bending, and arms over shoulders.