Objective:To study influence of type 2 diabetes mellitus (T2DM) on ambulatory blood pressure (ABP) levels and blood pressure variability (BPV) in aged patients with essential hypertension (EH) .Methods:A total of 80 aged EH +T2DM patients treated in our hospital from Jun 2014 to Jan 2016 were selected as observation group ,another 80 aged pure EH patients were selected as EH control group .The 24hABP indexes and BPV indexes were compared between two groups . Results:Compared with EH control group , there were significant rise in 24h mean systolic blood pressure [24hSBP , (126.47 ± 9.64) mmHg vs .(134.98 ± 10.63) mmHg] ,nighttime mean SBP [nSBP ,(125.73 ± 10.19) mmHg vs .(133.74 ± 11.29) mmHg] ,daytime mean SBP [dSBP ,(128.29 ± 10.97) mmHg vs .(136.42 ± 12.18) mmHg] ,daytime mean pulse pressure [dPP ,(59.46 ± 10.79) mmHg vs .(65.38 ± 11.69) mmHg] ,nPP [ (58.00 ± 10.11) mmHg vs .(67.14 ± 9.93) mmHg] and 24hPP [ (59.27 ± 10.16) mmHg vs .(64.76 ± 11.62) mmHg] , P<0.01 all;and significant rise in 24hSBP standard deviation [24hSBPSD ,(12.63 ± 2.87) mmHg vs .(18.41 ± 3.09) mmHg] and nSBPSD [ (10.14 ± 3.53) mmHg vs .(14.89 ± 3.54) mmHg] in observation group , P<0.01 both .Conclusion:Diabetes mellitus elevates daytime and nighttime SBP ,increases BPV and risk of cardiovascular diseases in aged EH patients .

A comparative study of systemic lupus erythematosus in 39 male(MSLE) and 120 female (FSLE) patients was carried out. The results showed that, in MSLE, the mean age at the time of disease onset was similar to FSLE, and the clinical features were nearly the same as those in females, except that the first signs of MSLE were less complicated than those of FSLE, malar rash occurred less commonly in MSLE than in FSLE(P

Objective To investigate the effects of Tianshu capsule on vasoactive substances in blood plasma or brain and hemodynamics in migraine animal models. Methods After subcutaneously administration of nitroglycerin (NTG) and tube feeding Tianshu capsule, the radioimmunoassay or spectrophotometry was used to measure the plasma contents of calcitonin gene related peptide (CGRP), nitric oxide (NO) and nitric oxide synthase (NOS) in rats.The distribution of NOS1, CGRP in rat nucleus tratus spinalis nervi trigemini was detected by immunohistostaining, and the changes of internal carotid artery flow velocity in rabbit were measured by transcranial Doppler.Results The levels of NO, NOS and CGRP were elevated in the plasma of migraine rats. Tianshu capsule could inhibit these increases, and the moderate-and high-dose had much significant effects (P

Objective To investigate the effects of Tianshu capsule(TSC) on the plasma levels of ?-endorphin(?-EP) and 5-Hydroxytryptamine(5-HT) and c-fos expression of brain tissue in the rat with migraine.Methods The rat migraine model was established by subcutaneously administration of nitroglycerin.The intragastric administration dose of TSC was divided into three groups as follows:high dose(7.5 g/kg),middle dose(3.75 g/kg) and low dose(1.88 g/kg).TSC was administrated respectively in therapeutic and prophylactic model.The control group and model group were administrated corresponding dose of normal saline.The plasma levels of ?-EP and 5-HT in rat were measured by radioimmunoassay and HPLC respectively;the distribution of ?-EP,5-HT and c-fos in rats periaqueductal grey were evaluated by immunohistochemistry.Results Compared to model group,the plasma levels of ?-EP were increased remarkably in high dose TSC therapeutic and prophylactic groups,middle dose prophylactic group(all P

Objective In this research ,the effects of exogenous C 2-ceramide on the induction of apop-tosis of mesothelioma cells in vitro and related important proteins are investigated .Mtehods Mesothelioma cells were treated with various doses of C 2-ceramide for different duration .Cell viability were analyzed by cell count-ing kit-8 assay.Morphological changes of apoptosis of mesothelioma cells were observed by Diff Quik staining . Apoptosis of mesothelioma cells was also detected by Caspase -3 assay and lactate dehydrogenase(LDH)assay. Related important proteins involved in the signal transduction of apoptosis were detected by western blot .Results In vitro,C2-ceramide demonstrated a dose -and time-dependent inhibition of cell proliferation .Apoptotic bodies were observed by Diff Quik staining .The antiproliferative effect of 80 μm C2-ceramide was paralleled with an increase in Caspase -3 activity.LDH assay showed that C2-ceramide at a concentration of 80μm signifi-cantly promoted cells death .After treated by C2-ceramide,the expressions of Bax and the phosphorylated JNK in mesothelioma cells were increased , however , the expression of phosphorylated ERK 1/2 kinase was decreased . Conclusion Our results indicate that C 2-ceramide induced apoptosis of malignant mesothelioma cells in vitro . This anti-tumor affect is achieved by adjusting related important proteins .

Objective:To detect in vitro the effect of IFN? on HL 60 cells or ATRA resistant HL 60 cells Methods:MTT was used to measure the proliferation of HL 60 cells or ATRA resistant HL 60 cells The differentiation of HL 60 cells or ATRA resistant HL 60 cells was detected by NBT To detect the apoptosis of HL 60 cells or ATRA resistant HL 60 cells with Flow Cytometric Analysis Otherwise,the ATRA resistant HL 60 cells was induced by way of ATRA density gradually increasement Results:IFN? could inhibit the proliferation of HL 60 cells,especially accompanied with the ATRA IFN? not only induce the differentiation but also promote the differentiation of HL 60 cells or ATRA resistant HL 60 cells ATRA resistant HL 60 cells post treatment with IFN? were sensitive to ATRA Conclusion:Effect on differentiation of HL 60 cells was enhanced by IFN? IFN? also reverse the resistance of ATRA resistant HL 60 cells to ATRA

Objective To investigate the effect of salidroside on brain edema and neurological function in global cerebral ischemia-reperfusion injury in rats.Methods A total of 100 Sprague Dawley rats were randomly divided into sham operation,ischemia-reperfusion and salidroside 12,24 and 48 mg/kg groups (n =20 in each group),and than redivided into 6 h,24 h,72 h and 7 dsubgroups (n =5 in each subgroup).A rat model of global cerebral ischemia was established using the four-vessel occlusion method.Immediately after modeling,all groups were administered intragastrically for 7 days.The brain water content was quantitated by the wet-dry weight method.The neurological evaluation was performed using a neurological deficit score (NDS).Results After modeling both the ischemia-reperfusion group and all the salidroside groups had significant neurological deficit,and as time went by,it was improved gradually.Compared to the ischemia-reperfusion group at the corresponding time points,neurological deficit in all the salidroside groups was improved significantly (all P ＜ 0.05),and showing a dose-dependent trend.Compared to the salidroside 12 mg/kg and 24 mg/kg groups,neurological deficit in the salidroside 48 mg/kg group was improved significantly at 72 hours and 7 days (all P ＜ 0.05).The brain water contents began to increase at 6 hours after modeling in the the ischemia-reperfusion group and all the salidroside group.They reached the peak at 72 hours,and significantly higher than that in the sham operation group (all P ＜ 0.05).The brain water contents in all the salidroside group were significantly lower than those in the ischemiareperfusion group at 24 and 72 hours after modeling (all P ＜ 0.05) and showing a dosedependent trend.The brain water content in the salidroside 48 mg/kg group was close to that in the sham operation group at 7 days after modeling.Conclusions Salidroside may significantlydecrease brain edema and improve neurological function in global cerebral ischemia-reperfusion injury in rats,and it has a neuroprotective effect.

Background and purpose:As a tumor suppressor gene, ribosomal S6 kinase 4 (RSK4) plays important roles in inhibiting cell proliferation, migration and inducing cell apoptosis. However, the proteins interacting with RSK4 are still unknown. This study aimed to screen proteins interacting with RSK4 in breast cancer cell line MDA-MB-231 by lfag-tag affnity puriifcation and LC-MS/MS (liquid chromatography/mass spectrometry).Methods:The pcDNA3.1/EGFP-RSK4-Flag eukaryotic expression vector was constructed by inserting full lengthRSK4 gene into vector pcDNA3.1/EGFP-Flag. And then the recombinant plasmids were transferred into MDA-MB-231 cells. Real-timelfuorescent quantitative polymerase chain reaction (RTFQ-PCR) and Western blot were used to detect the expression of RSK4 in MDA-MB-231 cells. Affnity puriifcation and LC-MS/MS were applied to screen proteins interacting with RSK4, and the related action mechanism of RSK4 with its interacted proteins was detected based on bioinformatics gene ontology (GO) and ingenuity pathway analysis (IPA).Results:Twenty-four proteins, such as serine/threonine-pro-tein kinase 38 (STK38)/serine/threonine-protein kinase 38-like (STK38L), MOB kinase activator 2 (MOB2) and protein arginineN-methyltransferase 5 (PRMT5), were successfully identiifed by Flag-tag affnity puriifcation followed by LC-MS/MS analysis, which probably interacted with RSK4. Bioinformatics analysis of the identiifed proteins suggested the proteins interacting with RSK4 were involved in diverse biological pathways, such as apoptosis and cell migration. Conclusion:According to bioinformatics results of proteins interacting with RSK4 identiifed by affnity puriifcation and LC-MS/MS, biological networks of RSK4 are involved in apoptosis and migration in breast cancer cells.

Objective To evaluate the effect of cisplatin on analgesia with morphine in rats with incisional pain.Methods Forty-two adult male Sprague-Dawley rats,weighing 180-220 g,were randomly divided into 6 groups (n =7 each) using a random number table:normal saline group (group C),normal saline + Pglycoprotein inhibitor LY335979 group (group CL),normal saline + morphine group (group CM),cisplatin group (group S),cisplatin + morphine group (group SM) and cisplatin + morphine + LY335979 group (group SML).Cisplatin 2 mg/kg was injected intraperitoneally once every two days for 5 times in S,SM and SML groups,while the equal volume of normal saline was injected intraperitoneally in C,CL and CM groups.At 2 days after the end of administration,the incisional pain models were established.At 10 min after establishing the model,normal saline 2 ml was injected subcutaneously in C and S groups; LY335979 20 mg/kg was injected via the caudal vein and normal saline 2 ml was injected subcutaneously in group CL; morphine 2 mg/kg was injected subcutaneously in CM and SM groups; LY335979 20 mg/kg was injected via the caudal vein and morphine 2 mg/kg was injected subcutaneously in group SML.Cumulative pain score was used to evaluate analgesia.Results Compared with group C,cumulative pain scores were significantly decreased in group CM,and no significant change was found in cumulative pain scores in CL and S groups.Compared with group CM,cumulative pain scores were significantly increased in group SM,and no significant change was found in cumulative pain scores in group SML.Cumulative pain scores were significantly lower in group SML than in group SM.Conclusion Cisplatin can weaken analgesia induced by morphine in rats with incisional pain through enhancing P-glycoprotein function in the blood-brain barrier.

Objective To investigate the efficacy of ablative fractionated erbium: yttrium aluminum garnet (Er: YAG) laser in facial acne scars and enlarged pores. Methods Forty-one patients with mild to moderate pitted acne scars and 23 patients with enlarged pores were treated with 81 (9 × 9) bits of facula for 3 to 5 sessions at an interval of 1 month. For acne scars, the pulse duration was medium to long, energy at 800 to 1200 mJ, and number of stacking passes 4 to 8; for enlarged pores, the pulse duration was medium, energy at 800 to 1000 mJ and number of stacking passes 2 to 4. The clinical improvement was evaluated by 2 blinded dermatologists. Meanwhile, the satisfaction rate was self-assessed by patients. Three-dimensional (3D) micro-topography imaging system was used to evaluate the improvement in surface roughness. Results The efficacy reached 82.93% and 86.96% for ache scars and enlarged pores, respectively. The satisfaction rate was 88.80% and 91.30% in patients with ache scars and those with enlarged pores, respectively. After treatment, the Ra and Rz values, as the indicators of roughness, decreased by 18.74% and 21.01%, individually (P < 0.001) in 11 patients including 6 with acne scars and 5 with enlarged pores. Conclusion Ablative fractionated Er:YAG laser can efficiently resurface pitted ache scars and shrink enlarged pores.