Objective　To investigate the clinical significance and relationship among Pre-S2、6 common types of HBV serum immune markers(HBV-M)and HBV-DNA.Methods　Using PCR to detect HBV DNA and ELISA technique to detect Pre-S2,6 kinds of HBV-M in the meantime.Results　Positive infection rates of HBV DNA in 6 common types of the 1484 cases were 89.8%>55.6%>21.8%>7.5%>7.1%>6.8% respectively,namely the model (1)>(3)>(2)>(5)>(6)>(4);but the positive cases of HBV serum immune markers were in the order (2)>(1)>(3)>(4)>(5)>(6).The positive rates were significant difference between Pre-S2 and HBV DNA in (2)、(3) types (P<0.01) and no significant difference in other types(P>0.05).Conclusion　It is necessary to detect Pre-S2,HBV DNA and HBVserum immune markers,but HBV serum immune makers and Pre-S2 detected by ELISA only external indication and indirect evidence of HBV infection.While the detection of HBV DNA by PCR was direct evidence of HBV infection.Therefore they have their unique clinicl significance.

Objective To investigate immunological dysfunction of intestine mucosa barrier in a rat model of sepsis. Method Sixty Sprague-Dawley rats were assigned randomly(random number) into sepsis group (n = 45)and control group (n = 15). The animals in sepsis group were subjected to cecal ligation and puncture (CLP), whereas rats of control group underwent a sham surgery. The ileac mucosa and segments were harvested 3 h, 6 h and 12 hours after CLP, and the blood samples were collected. Pathological changes, protein levels of defensin-5 (RD-5) and trefoil factor-3(TFF_3) mRNA, lymphocytes apoptosis in the intestinal mucosa were determined. In an additional experiment, the gut-origin bacterial DNA in blood was detected. Results In the septic animals, in-testinal mucosa showed marked injury with loss of ileal villi, desquamation of epithelium, detachment of the lamina propria, hemorrhage and ulceration. Compared with control, the expression of TFF_3 mRNA and level of RD-5 pro-tein were decreased and the mucosal lymphocyte apoptosis increased (P < 0.05) in sepsis group. Compared with control group, the significant differences in RD-5 and TFF_3 mRNA appeared 3 hours after CLP and those differ-ences were progressively increased in 6 hours and 12 hours after CLP in sepsis group (P < 0.05, F of RD-5 = 11. 76, F of TFF_3 = 16.86 and F of apoptosis = 122.52). In addition, the gut-origin bacterial DNA in plasma de-tected was positive in all sepsis animals. Conclusions It suggests that immunological function of intestinal mucosa is impaired in septic rats and further worsened following the course of sepsis.