1.The Screening and Study of Arctic Marine Psychrophilic Bacterial for Anti-tumor and Antibacterial Activity
Hong-Qiang SHANG ; Tian-Jiao ZHU ; Qian-Qun GU ; Wei-Ming ZHU ; Hua-Shi GUAN ;
Microbiology 1992;0(05):-
In this study, 101 strains of bacteria were isolated from arct ic water and sediment samples. The methanol extracts of the fermented broth prod uced by these strains were screened in vitro for anti-tumor activity on mou se tsFT210 cells using the method of flow cytometry, and screened for antibacter ial activity by the method of paper disk diffusion. The result showed that one strain exhibited anti-tumor activity and eight strains had antibacterial activ ity. The stability of the antibacterial components produced by strain AR084 an d its optimum medium were also studied. The research indicated that arctic bac teria had potential application in pharmaceutics.
2.Antitumor screening of deep ocean water and sediment derived fungi and primary investigation of their secondary metabolites
Boyu ZHAO ; Tian-Jiao ZHU ; Yu FANG ; Gu CHUN ; Qun QIAN ; Wei-Ming ZHU ;
Chinese Journal of Marine Drugs 1994;0(04):-
Objective The cytotoxic microbial strains isolated from the deep ocean water and sediments were screened,and the secondary metabolites of bioactive fungus c2b were investi- gated.Methods Active bioactive microbial strains were screened using brine shrimp and chro- nic medulla leucocythemia leukocythemia(K562)cell line.The cytotoxic components of fun- gus c2b were isolated by bioassay-guided fractionation and solvent extraction,silica gel col- umn chromatography and preparative HPLC.Their structures were established by pbysico- chemical properties and spectral analyses.The cytotoxicities of compounds were evaluated by SRB method.Results and Conclusion Twenty-nine strains of fungi were isolated.Among them,seven strains showed cytotoxic activities.Six compounds(1~6)were isolated and i- dentified as N-acetyl histamine(1),chrysogine(2),ergosterol peroxide(3),5,8-epidioxy- 24-methylcholesta-6,22-dien-3?-ol(4)cerevisterol(5)and(4E,8E)-N-[(2'R,3'E)-2'-hy- droxy-3'-hexadecenoyl]-1-O-?-D-glycopyranosyl-9-methyl-4,8-sphingadiene(6),respective- ly.Compound 3 and 4 showed median cytotoxicity.
3.Clinical Cross Sectional Study of Herpes Simplex Virus Infection of Central Nervous System in Newborn Infants
tian-jiao, YANG ; qi-rong, ZHU ; xiao-hong, WANG ; jian-she, WANG
Journal of Applied Clinical Pediatrics 2004;0(12):-
Objective To investigate the prevalence of herpes simplex virus(HSV) infection of central nervous system(CNS) in newborn infant,and analyze its clinical characteristics.Methods Cerebrospinal fluid(CSF) was collected from 40 acute viral infection of central nervous system who were hospitalized during June 2001 to June 2002.Polymerse chain reacton techniques(nested-PCR)was used to detect HSVspecific DNA in CSF,enzyme-linked immunosorbert assays(ELISA)was applied to detect HSV-specific IgM and IgG antibody in CSF and serum specimens.Results Two cases of neonatal patients were HSV-1 DNA PCR positive in CSF,both(mo)-ther were normal during pregnancy without a history of genital herpes.Clinical presentations of one case belonged to disseminated HSV infections and the other was limited to CNS infections.HSV-2 DNA PCR of 40 cases of neonatal patients were negative in CSF.Conclusions The rate of HSV neonatal CNS infection was 5% among viral neonatal CNS infections.HSV type 1 in the period,which showed that HSV type 1 may be the common type of HSV neonatal CNS infections.The result seems to be related to low prevalence for HSV-2(among) pregnancy women in China.
4. Prediction of functional connectivity of fronto-limbic network in the outcome of subjects with clinical high-risk for psychosis
Journal of Shanghai Jiaotong University(Medical Science) 2020;40(9):1222-1228
Objective: To explore the role of functional connectivity (FC) within the fronto-limbic network in predicting the onset of psychosis in the subjects with clinical high-risk for psychosis (CHR). Methods: A total of 164 CHR subjects and 89 healthy controls (HC) who underwent resting-state functional MRI were recruited. FCs between frontal [medial prefrontal cortex and bilateral or-bitofrontal cortex (OFC)] and limbic (bilateral hippocampus and bilateral amygdala) brain regions at baseline were calculated. CHR subjects were further divided into 3 subgroups, i.e., CHR converters (CHR-C) group, symptomatic CHR (CHR-S) group and remitted CHR (CHR-R) group according to clinical outcome after one-year follow-up. The FCs of fronto-limbic network were compared between the groups and among the subgroups, and their interaction with brain regions, as well as their correlations with positive and negative symptoms were analyzed. Results: There was no significant main effect of group (P=0.110), but a significant interaction of subgroups and brain regions (P=0.049). CHR-C group had lower FC between bilateral OFC and bilateral amygdala than HC group and CHR-R group (all P<0.05). The FCs between left OFC and left hippocampus in the three CHR subgroups were all lower than that in HC group (all P<0.05). In addition, the FC between left OFC and right amygdala was positively correlated with the severity of negative symptoms in CHR-C group, while the FCs between left OFC and bilateral hippocampus and between right OFC and left hippocampus were negatively correlated with the severity of positive symptoms in CHR-C group. Conclusion: The decrease of OFC-hippocampus connectivity may be common in the subjects with CHR, while the decrease of OFC-amygdala connectivity may predict CHR subjects will convert to schizophrenia in the later stage.
5.Research progress on triterpenoids of Betula plants
Yan-xin LI ; Ting GONG ; Jing-jing CHEN ; Tian-jiao CHEN ; Jin-ling YANG ; Ping ZHU
Acta Pharmaceutica Sinica 2023;57(5):1211-1220
The secondary metabolites of plants are important sources of natural drugs.
6.Etiology and treatment of vitreous hemorrhage in children
Zhengwei LIU ; Ping FEI ; Jie PENG ; Jiao LYU ; Jingjing LIU ; Tian TIAN ; Xin LI ; Xuehao CUI ; Kaiqin YU ; Xiuyu ZHU ; Peiquan ZHAO
Chinese Journal of Ocular Fundus Diseases 2017;33(4):434-437
Vitreous hemorrhage in children is caused by trauma or non-traumatic factors.Long-term vitreous hemorrhage not only affects children's vision,but also can lead secondary glaucoma,traumatic retinal detachment and other serious complications.Ocular trauma,some ocular and systemic diseases are the common etiology leading to vitreous hemorrhage in children.A small amount of vitreous hemorrhage can be treated by observation and conservative treatment.However,if the vitreous hemorrhage has no obvious absorption or serious complications appeared,it needs to be treated by surgery.The choice of treatment time and methods need to be further studied.
8.Association of TIAM1 gene polymorphisms with Kawasaki disease and its clinical characteristics.
Xian WANG ; Tian-Jiao ZHU ; Xiong-Fei ZHOU ; Zhi-Ting WAN
Chinese Journal of Contemporary Pediatrics 2015;17(11):1217-1220
OBJECTIVETo investigate the association of single nucleotide polymorphisms (SNP) rs22833188 and rs2833195 in TIAM1 gene with the susceptibility to Kawasaki disease (KD) and its clinical characteristic in children.
METHODSA case-control study was performed in this study. One hundred and eighty-eight children with KD and 197 normal children served as controls were enrolled. The genotypes of two SNPs rs22833188 and rs2833195 in TIAM1 gene were detected using PCR-RFLP.
RESULTSThere were no significant differences in the genotype (AA, AG and GG) and allele frequencies of SNP rs2833188 between the KD and control groups. Significant differences in the genotype (CC, GC and GG) frequency of SNP rs2833195 were noted between the KD and control groups (P=0.017). The frequency of C allele in the KD group was higher than in the control group (P=0.015). The polymorphism of SNP rs2833188 was associated with the occurrence of rash (P=0.011), and the polymorphism of SNP rs2833195 was associated with the occurrence of conjunctival hyperemia (P=0.021).
CONCLUSIONSThe polymorphism of rs2833195 in TIAM1 gene is associated with the susceptibility to KD. The polymorphisms rs2833188 and rs2833195 in TIAM1 gene may be associated with some clinical characteristics in children with KD.
Child ; Child, Preschool ; Female ; Genetic Predisposition to Disease ; Genotype ; Guanine Nucleotide Exchange Factors ; genetics ; Humans ; Infant ; Male ; Mucocutaneous Lymph Node Syndrome ; genetics ; Polymorphism, Single Nucleotide ; T-Lymphoma Invasion and Metastasis-inducing Protein 1
9.Current situation and consideration of medical instrument clinical trial in China.
Zhong-Ge XIAO ; Li-Ming ZHOU ; Zhou-Ping TIAN ; Jian JIN ; Hui ZHU
Chinese Journal of Medical Instrumentation 2009;33(5):369-371
OBJECTIVETo improve the quality of medical instrument clinical trial in China.
METHODSA systematic analysis on current situation of medical instrument clinical trial in China is conducted to find a way out of problems.
RESULTS & CONCLUSIONSThere is a low level of medical instrument clinical trial now in China. There are still many shortcomings in law and regulations of medical instrument clinical trial. The sponsors and s do not know medical instrument clinical trial well, the measures of quality related to medical instrument clinical trial must be improved.
China ; Clinical Trials as Topic ; standards ; Equipment and Supplies ; Humans
10.A study on the determination of the total flavonol glycosides in health food
Zhu-Nan NI ; Ying TAN ; Cun YU ; Jing ZHANG ; Tian-Jiao WANG
Journal of Preventive Medicine 2017;29(6):574-578
Objective To develop a HPLC method for the determination of the total flavonol glycosides in health food by acid hydrolysis. Methods The sample was extracted by methanol and was hydrolyzed into 3 aglycones by acid hydrolysis. They were quercetin, kaempferide and isorhamnetin. Chromatographic separation was carried out by the method of HPLC. The content of total flavonol glycosides was calculated. Results Methanol -25% hydrochloric acid solution (4:1) was added into selected samples, then 85 ℃ water bath heating and refluxing were conducted for 30 min. Optimum chromatographic conditions were as follows. Chromatographic column: ODS C18 (150 mm ×4.6 mm,5μm) . Mobile phase: used methanol-0.6% phosphoric acid (47:53) . Column temperature was set at 35 ℃. Detection wavelength was 360 nm. The linear ranges of quercetin, kaempferide and isorhamnetin were (4.766-95.52) μg/mL(r=0.9991) , (5.052-101.0) μg/mL (r=0.9994) and (2.027-48.66) μg/mL (r=0.9994) respectively. The relative standard deviation of the samples was 0.57%. The recovery rate was 98.25% (RSD=3.82%), 99.81% (RSD=3.31%) and 101.8% (RSD=4. 86%) . The detection limits of quercetin and kaempferide were both 0.50 μg, while Isorhamnetin was 0.80 μg, the total flavonol glycosides was 4.52 μg. The content of total flavonol glycosides detected by this method was lower than the content of the total glavonoids detected by the method provided by technical specification for inspection and evaluation of health food (2003 Edition) . Conclusion This method proved to be simple, stable and had strong repeatability and could be used for the determination of total flavonol glycosides in health food.