Objective To discuss the significance and alteration of heart rate turbulence (HRT) of diabetics with ventricular premature beats of coronary heart disease (CHD). Methods 107 patients were chosen and divided into DM group(n=30), CHD group (n=32)and normal group(n=45).The turbulence onset(TO)and turbulence slope(TS) were calculated and analyzed. Results For the diabetics with ventricular premature beats, TO value was higher and TS value was lower than the normal group(all P<0.05).The value of TO and TS of the diabetics with ventricular premature beats were similar to those of CHD(P>0.05). Conclusions HRT can be used to estimate the integrality and stability of cardiac autonomic nerve function for the diabetics and it is an important factor for diagnosis.

Images corresponded to the right knee of a 27 years old, healthy, male volunteer, weighing 68 Kg, 175 cm height, with no knee joint disease, were selected. The scanning was performed using 1.5T Twinspeed/Excite magnetic resonance tomography. The image was uninterrupted sagittal planes picture, and the scanning way was from the inside to out. The three-dimensional tibia-femoral joint model was established by the MR1 images after they were handled by correlation analysis software (Mimics10.0, Geomagic Studio 8.0). At last, transformed the model format STL file to IGS file, which can be acceptable by analysis software 11.0. On the base of this way, a highly simulation tibia-femoral model was established. It provides a guarantee for the further finite element analysis concerning checking the surface of femoral condyle cartilage to repair interphalangeal joint injuries. The result demonstrated that 3-D computer model established by using 2-D MRI pictures makes up the CT scanning deficiencies on soft tissue, which confirms the model closer to the real anatomical structure.

Objective To discuss the technique, therapeutic effect and complications of CT-guided interstitial implantation of ~(125)I seeds for the treatment of non-small cell lung carcinoma (NSCLC). Methods With the help of treatment planning system, CT-guided interstitial implantation of ~(125)I seeds was performed in 21 patients with pathologically-proved NSCLC. Multi-plane implanting technique was adopted and ~(125)I seeds of (0.5 -0.8) mCi were placed at intervals of (1.0-1.5) cm. Checkup CT scan was carried out immediately after the procedure to make sure that the seeds were satisfactorily distributed. Follow-up CT scanning was made (2-6) months after the treatment to survey the distribution of the seeds within the tumor. The curative effect and complications were also evaluated and observed. The patients were followed for 12 months. Results Of 21 cases, complete remission was seen in 7, obvious remission in 12 and partial remission in 2. The one year survival rate was 90.5% (19/21). Complications included pneumothorax (n = 6) and hemoptysis (n = 2). No serious complications or treatment-related radiation injuries occurred. Conclusion CT-guided interstitial implantation of ~(125)I seeds is a safe and effective treatment for NSCLC with reliable short-term therapeutic effect.

Carboxysomes are extremely efficient microcompartments committed to CO2 fixation due to tailored CO2-concentrating mechanism (CCM). In cyanobacteria and some chemoautotrophs, carboxysomes as organelle-like microbodies encapsulate ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) and carbonic anhydrase (CA). Together with active inorganic carbon uptake transporters, carboxysomes accumulate HCO3(-) in the cytoplasm, leading to high efficiency of carbon fixation. Based on the elucidation of structures and functionalities, heterologous production of carboxysomes has been achieved so far. In fact, the genes encoding either vacant carboxysome shell or only interior components have been characterized. This review summarizes the discovery along with types, showcases molecular structures and roles of carboxysomes in CCM, and presents their broad applications in metabolic engineering.
Biological Transport ; Carbon ; metabolism ; Carbon Cycle ; Carbon Dioxide ; metabolism ; Cyanobacteria ; metabolism ; Metabolic Engineering

Objective To investigate the correlation between the severity of coronary artery disease with se-rum bilirubin and left ventricular cardiac function. Methods Two hundred and six patients underwent blood bio-chemical ,color-echocardiography and angiography of coronary artery. The patients were divided into 4 ordinal groups according to the Gensini score indicating the severity of coronary artery disease. Results There were significant differences in total bilirubin(TBIL), left atrial dimension (LAD), left ventricular dimension (LVD) and left ventric-ular ejection fraction (LVEF) between the four groups(P<0.05 or P<0.01). The Spearman correlation coefficent between Gensini's scores and LAD, LVD were 0.280 (P=0.000) and 0.176 (P=0.011), respectively. The Spearman correlation coefficient between Gemini's scores and TBIL, LVEF were -0.541 (P<0.000) and -0.222 (P=0.001), respectively. The multiple linear regression between the Gensini's score and parameters showed that TBIL and LVD were independently correlated with the Gensini scores (P=0.000 and P=0.002, respec-tively). Conclusions TBIL and LVD were independently correlated with Gensini's score. LVEF was negatively cor-related with the severity of coronary disease and is a useful predictor for the severity of coronary atherosclerotic heart disease.

Objective To examine the impact of note-taking habits on academic scores of junior middle school students.Methods A sample of 412 junior middle school students were applied a self-developed note-taking habits inventory (NTHI) ,and the impact of note-taking habits on academic scores was examined by using bivariate correlation and linear regression analysis.Results Girls got significantly higher NTHI scores than boys [(3.67±0.55)vs(3.19±0.80),P＜0.01] ,and students from key classes got significantly higher NTHI scores than those of typical classes[(3.56±0.64)vs (3.37±0.75),P＜ 0.05].Total academic scores of Chinese,mathmatics and English was found significantly and positively related to total NTHI scores and the five subscales(r=0.280,P＜0.01 ;r=0.293,P＜ 0.01 ;r=0.126,P＜0.05 ;r=0.255,P＜0.01 ;r=0.239,P＜0.01 ;r=0.280,P ＜ 0.01).Attitude towards note-taking,full note-making and immediate note-rewriting showed direct and significant influence on total academic score of Chinese,mathmatics and English.Full note-making,attitude towards notetaking and immediate note-rewriting showed direct and significant influence on score of Chinese.So did of active note-making,immediate note-rewriting,full note-making and attitude towards note-taking on mathmatice scores,attitude towards note-taking and full note-making on score of Chinese.Conclusion Note-taking habits could be direct predictor of their academic scores for junior middle school students.

Objective To evaluate laparoscopy assisted radio frequency ablation (LRFA) and percutaneous radiofrequency ablation ( PRFA ) for the treatment of hepatocellular carcinoma ( HCC ).Methods A total of 525 HCC patients were enrolled,including LRFA group of 78 cases with 106 tumor nodules,and PRFA group of 447 cases with 565 tumor nodules.Results ( 1 ) In LRFA group complete ablation rate was 97.17％ (103/106); in LRFA group complete ablation rate was 93.09％ (526/565)(x2 =2.523,P =0.112).(2)The 1,3,5 year overall survival rate was 96.15％,55.12％ and 38.46％in LRFA group and 93.73％,48.54％ and 31.54％ in PRFA group respectively ( x2 =0.699,1.151,1.447 ; P =0.403,0.283,0.229 ).The 1,3,5 year disease-free survival rate was 94.87％,43.58％ and 28.21％ in LRFA group and 93.73％,48.54％ and 31.54％ in PRFA group respectively (x2 =0.915,0.303,0.174; P=0.339,0.582,0.676).The average disease-free survival time was 22.25 months in LRFA group and 21.53 months in PRFA group respectively.(3)The serious complications was 0％ (0/78)in LRFA group and 1.34％ ( 6/447 ) in PRFA group respectively.( 4 ) Recurrence rate was 23.07％(18/78) in LRFA group,and 34.89％ (156/447) in PRFA group ( x2 =4.189,P =0.041 ).Conclusions The therapeutical effect of LRFA equals that of PRFA,while enjoying lower recurrence rate,no serious complications and higher treatment safety.

Objective To investigate the effectiveness of intensive sit-to-stand training in improving the balance and walking of hemiplegic stroke patients. Methods Sixty hemiplegic stroke patients were randomly divided into an observation group and a control group with 30 cases in each.Both groups were treated with routine rehabilitation.The observation group also underwent extensive sit-to-stand training.The training lasted for 15 min/time,6 times/week for 4 weeks.All of the patients were assessed with respect to their body weight distribution,Berg balance scale (BBS) scores,timed up & go test (TUGT) times and using footprint gait analysis,before the treatment and 4 weeks post treatment. Results After 4 weeks of treatment body weight distribution,BBS scores,TUGT times and gait improved significantly in both groups.The BBS,TUGT and gait improvements were significantly greater in the observation than in the control group.There was no significant difference between the two groups in terms of body weight distribution. Conclusions Extensive sit-to-stand training combined with routine rehabilitation can distinctly improve the balance and walking of hemiplegic stroke survivors.

Urine glycosaminoglycans of two patients (full brother and sister )with Sanfilippo's syndrome were analysed by electrophoresis, enzymatic or chemical treatment and ion-exchange chromatography. Heparin sulfate is the main glycosaminoglycan accouting for 70% and 58% of urine glycosaminoglycans in both patients, whereas only 29% and 42% of urine glycosaminoglycans are cnondroitin sulfate. Tbe C/O ratio urine heparin sulfate of the patients is 2.32, indicating that the hexuronic acid component parts of the patient's heparin sulfate are much the same as general heparin sulfate. However, the analysis of glycosaminoglycans with DEAE-Sephadex A-25 chromatography and electrophoresis in 0.1 M HCL showed that urine heparin sulfate of the patients arc heterogeneous, and that it covers, at least, two molecular types with different amount of sulfate and charge density.

AIM To investigate the effect of resveratrol on human liver cancer HepG2, and to study the function of resveratrol in inducing HepG2 apoptosis. METHODS HepG2 cells were treated with resveratrol at various concentrations. MTT assay was used to examine growth and proliferation of HepG2 cells after treatment with resveratrol for 24 hours. HE staining and electromicroscope were used to observe the morphology of apoptotic cells, and TUNEL, flow cytometry were also used to analyze the HepG2 apoptosis. RESULTS Resveratrol significantly inhibited growth and proliferation of HepG2 cells in dose dependent manner. And resveratrol also induced the apoptosis of HepG2 cells. CONCLUSION Resveratrol may inhibit HepG2 cell growth and proliferation through inducing apoptosis.