Retinoblastoma (RB) is a common intraocular malignant tumor of infants.It not only seriously threats children's eyesight,but also endangers their lives.RB develops from the immature cells of retina and its occurrence is closely related with the tumor suppressor gene RB1.The inactivation of two alleles of RB1 is the basis of RB occurrence and development.With the rapid development of biological technology,RB gene related research has made great progress.Researches showed that there are many changes in the chromosome level of RB.Many genes are also involved in the development and progression of RB,including oncogene MYCN,murine double minute 4 (MDM4,also known as MDMX),driver protein family members 14 (K IF14),DEK,E2F3,tumor suppressor gene calcium 11 (CDH11) and so on.This review summarizes the progress in gene research of RB,reveal pathogenesis of RB on DNA molecular level and provides a scientific basis for clinical doctors to formulate effective therapeutic plans.

Surgery and endovascular techniques are the most important methods in treating lower limb atherosclerosis occlusion.Different strategies should be adopted according to the features of lesion in lower limb.This article reviews the latest clinical outcomes in treating lower limb atheroselerosis occlusion with surgery and endovascular techniques.

Objective To observe the effect of treating child anorexia belonging to deficiency of spleen and stomach type with Jianpichongji. Methods A total of 100 children with anorexia were randomly recruited into a control group (40 cases)and a treatment group (60 cases). The control group was treated with Shibeigao ganule, and the treatment group was treated with Jianpichongji. Therapeutic effects between the two groups after the treatment were compared. Results The total effective rate was 90.0% and 77.5% in the treatment group and the control group respectively, showing statistical difference (P=0.035).Conclusion It was effective to treat child anorexia belonging to deficiency of spleen and stomach type with Jianpichongji.

Objective To observe the change of peripheral blood lymphocytes subsets in patients with active ulcerative colitis (UC) and explore its clinical significance. Methods The clinical data and lymphocyte subsets of 40 active UC patients who admitted to the Department of Gastroenterology, Peking University First Hospital,from June 2007 to January 2010 were retrospectively analyzed. Patients who had previously used immunosuppressants or tumor necrosis factor monoclonal antibody were excluded. Seventy-nine subjects with health examination served as controls. Peripheral blood lymphocyte subsets detected included total T cells, CD4 + T cells, CD8 + T cells, B cells, and NK cells. Only the first detection results of UC patients were used for analysis. Results The proportion of total T cells for UC patients (73.60% ± 8.35% ) was significantly higher than the controls (69. 76% ±7.37%) (P =0.012). CD8+T cell ratio (35.53% ± 10.99%) was significantly higher than the controls ( 30. 56% ± 6. 75 % ) ( P = 0. 011 ). When the UC patients were stratified according to inflammatory involvement,the total T cells, CD8 +T cell, and NK cell ratio were significantly different among pancolitis, non-pancolitis, and the controls ( all P ＜ 0. 05 ). When the UC patients were stratified according to the disease course, the total T cells and CD8 + T cell ratio were significantly different among chronic recurrent/persistent, onset, and the controls ( both P ＜ 0.05 ). When the UC patients were stratified according to the extraintestinal manifestations, the total T cells and CD8 + T cell ratio were significantly different among those with extraintestinal manifestations, those without extraintestinal manifestations, and the controls ( both P ＜ 0.05 ). Conclusions The proportion of total T cells and CD8 + T cells increase in UC patients. Such immune abnormalities are even more distinctive in those UC patients who suffer from pancolitis or chronic recurrent/persistent type or those with extraintestinal manifestations.

Objective To explore the effect of PCEA on intestinal peristalsis.Methods 120 patients with ASA Ⅰ～Ⅱ lower abdominal or lower limbs surgery were randomly divided into four groups with 30 cases each and received PCEA at the end of operation sespectively.Group A were given 0.0002% fentanyl,0.2% Ropivacaine and 0.005 % Droperidol;0.01% morphine,0.2% Ropivacaine and 0.005% droperidol in group B;0.2% Ropivacaine in group C;0.9% saline in group D.Postoperative analgesia was provided by PCA pump with LCP model.Results Satisfactory analgesia in group A was 93%,97% in group B 47% in group C and 13% in group D.Both group A and B did not show any significant difference in the analgesic effects(P＞ 0.05).Satisfactory analgesia in group A and B were significantly better than in group C and D(P＜0.05).The renewal of intestinal peristalsis were significantly faster in lower limbs surgery patients than lower abdominal patients(P＜ 0.01),but it did not show any significant difference in the same kinds of surgery in four groups (P＞0.05).Conclusion Both group A and B can get a satisfied analgesia effect;intestinal peristalsis of lower limbs surgery patients renewed were shorter than in lower abdominal patients.

Abnormality of sphincter of Oddi is divided into the anatomical and functional ones. Anatomical abnormality includes stenosis and insufficiency, and the dyskinesia of sphincter of Oddi is composed of hypermotility and hypomotility. Many biliopancreatic diseases are associated with the abnormality of sphincter of Oddi, which include congenital pancreaticobiliary maljunction, gallstones, primary choledocholithiasis and hepatolithiasis, post-cholecystectomy syndrome, and pancreatitis. The proper acknowledgement of abnormality of sphincter of Oddi is of importance to improve the diagnosis and therapy of corresponding biliopancreatic diseases.

New method of prostate cancer has grown more precise with the development of medical imaging.Multi-parametric MRI targeted biopsy and fusion biopsy reduce the cores,at the same time they have similar positive rates compared to systematic biopsy.The most used biopsy methods are still transrectal guided transrectal biopsy and transperineal biopsy in China.If we can predict biopsy result before the operation by physical and laboratory examination,it will help improve the detection rate and avoid repeating biopsies.And it has been indicated that age,tPSA,PSAD,fPSA/tPSA,PV,DRE,PHI and PCA3 are the predicting factors.Chinese Prostate Cancer Consortium Risk Calculator is fit for Chinses people.

This article introduces the explorations in improving minimally invasive therapies and techniques for intra-and extrahepatic bile duct stones in this center,including laparoscopic choledocholithotomy without the placement of T tube,laparoscopic partial hepatectomy for the treatment of intrahepatic bile duct stones using an anastomosis stapler for liver partition,and modification of percutaneous transhepatic cholangioscopy.The improved therapies and techniques above have achieved good clinical effects,and a scientific judgment of their clinical effects still depends on a large number of cases and randomized controlled clinical trials.

BACKGROUND:Three-dimensional printing technique has been applied in medical fields since it was invented in the end of 20th century. Recently it has been widely used in manufacturing scaffolds for bone tissue engineering. OBJECTIVE:To review the basic concept of the scaffold for bone tissue engineering, the basic requirements for three-dimensional printing technique in scaffold engineering, different materials used in bone tissue engineering, the advantages and limitations of three-dimensional printing technique and the outlook of three-dimensional printing technique applied in manufacturing scaffold for bone tissue engineering. METHODS: The first author did a computer-aided retrieval of the MEDLINE database, Science Direct database, CNKI database, and CQVIP database for articles relevant to three-dimensional printing technique used in manufacturing scaffolds for bone tissue engineering published between January 1990 and February 2015. The key words were three-dimensional printing, tissue engineering, rapid prototyping technology, scaffold, materials in English and Chinese, respectively. Repetitive studies were excluded, and 33 of 52 related literatures were adopted in result analysis. RESULTS AND CONCLUSION: Three-dimensional printing technique has many advantages such as high resolution, high velocity and the freedom to build unlimited geometries. There are some requirements for the powder and binder used to construct bone tissue engineering scaffolds using the three-dimensional printing technique, such as the flowability, stability and wettability. A wide range of materials can be used: synthetic and natural polymers, ceramics, as wel as composites of the aforementioned. Various kinds of powder take responsibility of different features of scaffolds, resulting from the advantages and disadvantages of different materials. Although this technique has some limitations such as high cost and the difficulty of commercial production, its application stil has a bright future.

Preterm birth is the main factor affecting the lives and quality of life for premature infants,which is also the main cause for a large economic burden on family,society and medical system.It has been confirmed that the bad neurodevelopment outcome can be effectively prevented by early intervention programs,which are including therapeutic developmental interventions targeting the infants,as well as psychosocial support and education for parents.This review summarizes early intervention programs for improving developmenr outcome in recent years.