Excellent mutilmedia courseware is the base to ensure teaching effect. Making excellent mutilmedia courseware by Director can make mutilmedia courseware more beautiful and have much more interaction and expressive force.

In order to further promote friendly cooperation with developing countries, Chinese government has been committed to increasing the intensity of cooperation and human resource development among developing countries. Healthcare is one of the key areas of China’s foreign aid. This article described the general conditions and experiences of national foreign aid training projects for developing countries undertaken by the Beijing Traditional Chinese Medicine Hospital and Beijing Traditional Chinese Medicine Research Institute. From aspects of participant enrollment, training program implementation, and training feedback, the training mode of national foreign aid on traditional medicine was introduced. This article focused on the selection of teachers, preparation of professional textbooks, and evaluation of teaching quality. Training programs over the years were guided by clear training concepts and accurate goal settings, which provided a useful exploration for the personnel training mode of national foreign aid on traditional medicine.

Objective To evaluate the shaping ability of ProTaper Universal (PU), ProTaper Next (PN) and M3 in preparation of curved root canals by CBCT (cone-beam computed tomography). Methods Sixty extracted maxillary first premolars were divided into two groups according to the canal curvature:10°-25° group and 26°-75°group. Then, each group was divided into PU group, PN group and M3 group and prepared with three instruments respectively. Canals were scanned by CBCT scanner before and after preparation. The efficacy of canal preparation was analyzed. The width of dentine removed was measured at three points (2 mm, 5 mm and 8 mm to root tip). The apical transportation indexes and the centring ratio values were recorded. Results PN group and M3 group needed less time in preparation and showed more efficiency than those of PU group in 26°-75° groups. One of root canals in PU group appeared deformation, neither PN group nor M3 group appeared deformation after 5 root canal preparations in average. None instrument was broken in three groups. PU group removed more materials at internal walls than PN group and M3 group at internal walls of root canals in 5 mm from the apex. There was no difference in the offset of the external walls of root canals. Compared with the offset of the internal and external walls of root canals in 2 mm from the apex, the apical transportations prepared by the PN group were less than those of M3 group and PU group in 26°-75° groups. Compared with the offset of the internal and external walls of root canals in 5 mm from the apex, the apical transportations prepared by the PN group and M3 group were less than those of PU group, and their centring ratio values were greater than those of PU group in 26°-75°groups. Conclusion ProTaper Next and M3 tested in this study are effective and time-saving in shaping curved root canals. The results indicate that both ProTaper Next and M 3are comparable to optimally enlarge root canal.

Objective To investigate the relationship between Pre-S1 antigen and hepatic function of the patients with chronic hepatitis B.Methods Serum samples of 300 cases with chronic hepatitis B patients were collected,Pre-S1 antigen and alanine aminotransferase (ALT),aspartate aminotransferase (AST) were checked,and the correlation were analyzed.Results Among 300 cases,patients with positive Pre-S1 antigen were 58.33％(175/300).In patients with positive Pre-S1 antigen,the abnormal ALT(＞40 U/L) detection rate was 78.75％ (138/175),the abnormal AST(＞40 U/L) detection rate was 77.17％ (136/175),Pre-S1 antigen negative patients ALT abnormal detection rate was 51.20％ (64/125),AST anomaly detection rate was 42.40％ (53/125).There was significant difference between two groups(x2 =2.875,5.223;P＜0.05).Conclusion In patients with chronic hepatitis B,Pre-S1 antigens can be used as a marker of hepatitis B virus infection,copy and hepatic cell injury.

Human papillomavirus is an oncogenic DNA virus and has more than 90 subtypes. High risk types (HPV 16, 18, 33) are associated with many kinds of malignant neoplasms of head and neck. The HPV DNA can induce mutations of p53, RB genes and increase cell transformation and over proliferation. The frequences of HPV positive are different in malignant neoplasms from different head and neck sites. HPV was more often detected in squamous cell carcinoma of oral cavity, oropharynx and larynx. In nasopharyngeal carcinoma, adenocarcinoma or lymphoma, the expression of HPV was quite low. The detection of HPV in cervical metastatic lymph nodes can improve diagnosis of primary sites.

The changes of lysosomes of the hepatocytes in 27 healthy female rats aged 1, 5, 15, 30, 50, and 90 days respectively were observed on morphometry and by studying the ultrastructures with electron microscope. The results are as following:1. The volume density and numerical density of the lysosomes of rat hepatocytes are markedly increased as the age increases.2. The lysosomes originally scattered in cytoplasm gradually migrate to the periphery of bile canaliculi.3. The proportion among certain forms of secondary lysosome changes as the rats grow older and the alteration of autophagic vacuoles is more prominant than others.The significance of the lysosome changes mentioned above was discussed. The author, considering from all the results, suggests that the time around five days after the birth of the rats is a critical period for the development of the lysosomes of rat hepatocytes.

Objective To discuss the expression and its significance of neuron specific enolase(NSE) and soluble-protein 100(S100) in Hirschsprung′s disease(HD) and its allied disease.Methods Clinicopathologic data were reviewed from 120 cases of suspected HD patients.According to the morphological characteristic of the nerve stanza cell stained with HE under the microscope.NSE and S100 immunohistochemical staining was performed in 29 cases.Then we compared the effect of the staining with analyzed the distribution.Results The number of the nerve plexus and ganglion cell of allied HD was significantly higher than that in normal control group(P

Acute myeloid leukemia (AML) originates from rare leukemia stem cells (LSC).Because these chemotherapy-resistant LSC are thought to underlie disease relapse,effective therapeutic strategies specifically targeting these cells may be beneficial.With the increasing knowledge regarding the LSC,several major directions in targeted therapies with regard to LSC are being investigated.These include targeting cell surface molecules,signal pathways,and microenvironment of LSC.Since eliminating LSC-should provide an efficient,potentially curative treatment option for leukemia patients,this article reviews the recent progress of LSC targeting in AML therapy.

Objective To observe the effects of pregnancy induced hypertension on lipid metabolism and bilirubin metabolism in term neonates.Methods 200 term neonates were selected and divided into the two groups,100 cases whose mother were healthy were control group and another 100 cases whose mother had gestational hypertension were observation group.Birth weight,measured in cord blood triglyceride (TG),total cholesterol (TC) levels,high density lipoprotein (HDL-C),low density lipoprotein (LDL-C),adiponectin (APN) level were recorded.Results Birth weight of the observation group had significantly lower birth weight (2 927 ± 492) g,than the weight of the control group (3 452 ± 348) g.The incidence of lower birth weight was higher than in the observation group.TG,LDL-C levels of the observation group were (0.6 ± 0.24) mmol/L,(1.95 ± 0.25) mmol/L,were higher than those of the control group[(0.39 ± 0.17) mmol/L,(1.95 ± 0.25) mmol/L].There was a statistically significant difference between the groups (t =12.35,2.35,P ＜ 0.01 or P ＜ 0.05).HL-C and APN of the observation group were lower than those of the control group (t =1.79,7.00,all P ＜ 0.05).Bilirubin after bomed 24 hours,48 hours,72 hours and bilirubin peak of the observation group were (8.64 ± 2.17) mg/L,(11.06 ± 3.12) mg/L,(10.32 ± 2.47) mg/L,(12.04 ± 2.13) mg/L and were higher than those of the control group [(3.06 ± 0.94) mg/L,(6.98 ± 1.56) mg/L,(7.26±1.89)mg/L,(9.64±1.98)mg/L](t=23.48,11.65,9.84,8.27,allP＜0.01).Conclusion Maternal PIH may affect birth weight,lipid metabolism and bilirubin metabolism.

Objective To assess the diagnostic value of 3D-CE MRA for nutcracker syndrome (NCS).Methods The 3D-CE MRA appearances of 4 patients of NCS and control group ( 30 persons) were analyzed.Results The left kidney vein (LKV) crossed before abdominal aorta in 4 NCS patients and 30 controls- The average angle of the origin of the superior mesenteric artery (SMA) from the abdominal aorta was (84.50±3.60)° in control group,and became small (<30°) in 4 NCS patients.The distance of the origin of the SMA to the beginning of descent was (13.40±5.10) mm,and the SMA and abdominal aorta in the axial plane crossing the LKV was (16.40±5.10) mm in controls,and all decreased in 4 NCS patients whose LKV was obviously compressed.The average ratio of expansion to stenosis caliber of the LKV was 4,5 in 4 NCS patients,whereas 1.4 in the controls.Conclusion 3D-CE MRA can display relation between abdominal aorta,SMA and LKV,thus exactly diagnose the nutcracker syndrome.