Bilirubin is the final product of aging red blood cells when they were destroyed and degraded. Recent studies have demonstrated that it is a kind of endogenous antioxidant, which plays an important role in anti-atherosclerosis, scavenging free radicals and protecting the tissues and organs from damage. More and more studies have focused on the correlation between bilirubin and ischemic stroke. This article summarized the relationship between the antioxidant system of bilirubin and the occurrence, development and prognosis of atherosclerosis and ischemic stroke.

Functional gastrointestinal disorders is a common functional disease without organic pathologic changes, mainly including functional dyspepsia, irritable bowel syndrome and functional constipation.As the dissatisfaction, side effects and recurrence with drug treatment, transcutaneous electrical stimulation has taken an important role in the treatment of functional gastrointestinal disorders in recent years.This article reviewed the progress of transcutaneous electrical stimulation in treatment of functional dyspepsia, irritable bowel syndrome and functional constipation.

To study the effects of Chinese herbal medicine Yinsiwei compound (YSW) on spatial learning and memory ability in rats with sporadic Alzheimer disease (SAD) and the ultrastructural basis of the hippocampal neurons.

Objective To investigate the efficacy and safety of percutaneous pericardial drainage with indwelling catheter via the puncturing route of left sternal broader in the third or fourth interspace Methods In the 38 patients with moderate to large pericardial effusion, the pericardial echo free space and the forecasted puncturing distance were measured with two dimensinal echocardiolgraphy (2DE) prior to pericardiocentesis in the three sites: subxiphoid, cardiac apex and the left sternal broader in the third or fourth interspace Under the guidance of 2DE, pigtail catheters were percutaneously indwelled for pericardial drainage via the location of left sternal broader in the third or fourth interspace Results Among the three sites, there were no difference of echo free space measured by 2DE, and the forecasted distance of advancing needle examined in left sternal broader was the smallest The procedures of indwelling catheter drainage for pericardial effusion were successfully performed in all the 38 patients Catheters lying in posterior pericardium were found in 94 8% of the patients (36/38) There were no cases with puncturing related complications, effusion leaking into pleurum or subcutaneous tissue Only 1 case was complicated with neural mediated syncope on the third day after indwelling catheter Conclusion Pericardiocentesis using left sternal broader route is a safe and simple approach for indwelling catheter drainage in patients with pericardial effusion This route may be a promising option for clinical use widely

Objective To explore the possible antiepileptic mechanism of Pingxian Powder. Methods In the experiment,Pentylenetetrazole (PTZ) was injected into abdomen of rat to make epileptic kindling model. The total number,percentage of positive area and integral optical density of N-methyl-D-aspartate receptor1 (NMDAR1) and ?-aminobutyric acid-A receptor (GABA-AR?1) of immunoreactive cells in the temporal cortex were studied by the immunohistochemical method and image analyzing system. Results The total number,percentage of positive area and integral optical density of NMDAR1 of immunoreactive cells in the temporal cortex of model group were more than normal group (P

Objective Though immunohistochemical study on the effects of Pingxian Powder on N-methyl-D-aspartate receptor1 (NMDAR1) and ?-aminobutyric acid-A receptor ?1 (GABA-AR ? 1 ) in central nervous system of pentylenetetrazol-induced epileptic rats, to explore its possible antiepileptic mechanism. Methods Sixty wistar rats were divided into control group (A), model group (B), phenobarbital group (C) and Pingxian Powder small (D), middle (E), Large (F) dosage group. Pentylenetetrazole (PTZ) was injected into abdomen of rat to reconstruct epileptic kindling model. The total number, percentage of positive area and integral optical density of NMDAR1 and GABA-AR ? 1 of immunoreactive cells in hippocampus CA 1 , CA 3 were studied by the immunohistochemical method and image analyzing system. Results The total number, percentage of positive area and integral optical density of NMDAR1 of immunoreactive cells in hippocampus formation of Group B were more than Group A (P

Objective To determine serum soluble interleukin 2 receptor level in patients with posthepatitis B liver cirrhosis.Methods Serum sIL 2R was measured using enzyme linked immunosorbent assay.Results Serum sIL 2R was significantly higher in patients with posthepatitis B cirrhosis than that in controls(P

Objective:To obtain mutA gene of Strepto c occus mutans (Ms),and to express it in E.coli DH5?.Methods: mutA gene was amplified by PCR with specific primers from genome of Ms CH43 strain. After sequencing, the gene segment was inserted into vector pProEX and expressed in E.coli DH5?.The protein expression was induced by ITPG an d the protein products were examined by 180 ml/L SDSPAGE electrophorosis. Results:The length of PCR product was 147 bp and was identical to mu tA gene reported by GenBank.The mutA gene product was expressed in E.col i DH5? with Mr of 5.7?10 3.The maximum mutA protein product amount (20% of the total bacterial protein) was obtained when the A 600 value of DH5? was 1.666,IPTG concerntration 1.0 mmol/L and induction time 6 h.Conclus ion:mutA of Ms CH43 can be cloned and expressed in E.coli DH 5?.

Objective Since predicting protein secondary structure is the basis of predicting protein spacial structure, it is important to improve the prediction accuracy of secondary structure. Methods A two-stage BP neural network was constructed based on the method of combining hydrophobicity of amino acid residues with PSSM which contains evolution information. CB513 dataset was employed in our study. After excluding the protein chains containing X,B and those with sequence length shorter than 30 amino acids, 492 protein chains in the dataset were used. The results of protein secondary structure prediction of our study were compared with those from the networks using only PSSN as their inputs. Accuracy of the network was tested by 4-fold cross-validation. Results In our study, α-helix was predicted with an averaged accuracy of nearly 79%, sensitivity of 79% and specificity of 91%. Total prediction accuracy of secondary structure reached 75.96%, which was higher than that of only using PSSM as input. Conclusion The new method developed can better predict protein secondary structure, especially α-helix with a higher accuracy.

Objective:To study the safety and efficacy of primary closure of non-dilaed common bile ducts (CBD) after laparoscopic common bile duct exploration.Methods:A retrospective study was conducted on the clinical data of 120 patients who underwent primary closure of CBD after laparoscopic CBD exploration (LCBDE) at the Shengjing Hospital of China Medical University from April 1, 2014 to November 30, 2019. There were 44 males and 76 females, aged 22.0 to 88.0 years, (average age of 57.2 years). These patients were divided into the dilated CBD group (diameter of CBD >8 mm) and the normal CBD group (diameter of CBD ≤8 mm). The following factors, including operating time, intraoperative bleeding, postoperative hospitalization stay, abdominal drainage tube indwelling time and postoperative complications were compared.Results:The dilated CBD group consisted of 76 patients, with 25 males and 51 females, and a median age of 62.5 years. The normal CBD group consisted of 44 patients, with 19 males and 25 females, and a median age of 57.5 years. There were no significant differences in gender, age, albumin level, total bilirubin and other baseline data between groups ( P>0.05). The operation times of the two groups were: the normal CBD group [106.6(87.3, 146.3] min vs the dilated CBD group [112.0(90.5, 134.5)] min; intraoperative blood loss [the normal CBD group 20(10, 30)ml vs dilated CBD group 20(10, 20)ml]; postoperative hospital stay [the normal CBD group 7.0 (5.3, 9.0) d vs the dilated CBD group 7.0 (5.0, 7.0) d]; and postoperative abdominal drainage tube removal time [the normal CBD group 6 (4, 7) d vs the dilated CBD group 5 (4, 6)d]. The differences were all not significant ( P>0.05). There was also no significant difference in the incidences of postoperative complications (including bile leakage, biliary stricture, and stone recurrence between groups, all P>0.05). Conclusion:In patients with a normal diameter (≤8 mm) common bile duct, it was safe and efficacious to perform primary closure after laparoscopic common bile duct exploration.