Attitude ; Humans ; Morals ; Research Personnel

Objective To investigate the associations of atrial natriuretic peptide(ANP)rs5065 and brain natriuretic peptide(BNP)rs198389 gene polymorphisms with human essential hypertension(EH).Methods A total of 976 patients with EH and 976 age-and sex-frequency matched normotensive(NT)control subjects were collected.The ANP rs5065 and BNP rs198389 polymorphisms were genotyped by real-time quantitative polymerase chain reaction(real-time Q-PCR)method--Taqman probe.Results There was significant difference in frequency distribution of AA and AG+GG genotypes,A and G allele of BNP rs198389 between the EH and NT groups(P＜0.05).The frequency of AG+GG genotype and G allele was significantly higher in the EH group(30.5% and 17.3%)than in the NT group(26.1% and 14.1%).There was no difference in distribution of ANP rs5065 polymorphism between EH and NT groups(P＞0.05).By unconditional Logistic regression analysis of rs198389,before adjusted for the EH risk factors,AG+GG genotype presented a significantly higher risk of EH(P=0.04)than AA genotype,and this risk was still significant after adjusted for the EH risk factors(P=0.02).G allele had more significantly increased risk of EH than A allele(P=0.04).However,there was no significant difference before or after adjusted for the EH risk factors in unconditional Logistic regression analysis of rs198389.Conclusion rs198389 polymorphisms of BNP gene may be associated with EH,but rs5065 polymorphisms of ANP gene may be not associated with EH.

OBJECTIVETo verify the clinical efficacy on post-stroke dysphagia treated with acupuncture at Lianquan (CV 23).

METHODSOne hundred and eighty patients were randomized into an acupuncture A group, an acupuncture B group and a rehabilitation group, 60 cases in each one. On the basis of the conventional medication, in the acupuncture A group, acupuncture was applied at Lianquan (CV 23); in the acupuncture B group, acupuncture was applied at Hegu (LI 4) and Neiguan (PC 6) and in the rehabilitation group, the swallowing rehabilitation training was adopted. The treatment was given once a day, 5 times a week, and the 4 weeks of treatment was required in all of the groups. The national institute of health stroke scale (NIHSS) and TV X-ray fluoroscope swallowing scale (VFSS) were used to evaluate neurologic deficit and swallowing function before and after treatment in the patients of each group. The morbidity of pneumonia and clinical efficacy were compared among the groups.

RESULTSThe scores of NIHSS and VFSS were improved apparently after treatment in the patients of the three groups (all P < 0.05) and the results in the acupuncture A group were superior to those in the other two groups (all P < 0.05). The morbidity of pneumonia in the acupuncture A group was lower than that in the acupuncture B group and the rehabilitation group [3.3% (2/60) vs 6.7% (4/60), 8.3% (5/60), both P < 0.05]. The effective rate in the acupuncture A group was better than that in either of the other two groups [95.0% (57/60) vs 81.7% (49/ 60), 75.0% (45/60), both P < 0.05].

CONCLUSIONOn the basis of the conventional medication, acupuncture at Lianquan (CV 23) effectively improves the swallowing function, relieves neurological deficit and reduces the morbidity of pneumonia in the patients of post-stroke dysphagia.

Acupuncture Therapy ; Aged ; Deglutition ; Deglutition Disorders ; etiology ; physiopathology ; therapy ; Female ; Humans ; Male ; Middle Aged ; Quality of Life ; Stroke ; complications ; Treatment Outcome

Erectile dysfunction (ED) results from the interaction of many pathological factors. Studies show that a high incidence of ED is associated with chronic diseases of various systems, and its pathogenesis is not fully understood. This article outlines the progress in recent studies on the impact of chronic diseases on erectile function.
Chronic Disease ; Erectile Dysfunction ; etiology ; Humans ; Male ; Penile Erection ; physiology

Objective: To prepare Lingyi Formula multicomponent microemulsion and evaluate its anti-lung cancer activity. Methods: Lingyi Formula multicomponent microemulsion was prepared by aqueous titration method using polysaccharides solution of Ganoderma lucidum and Coix lachryma-jobi var. ma-yuen as aqueous phase and coix seed oil as oil phase, loading ganoderma triterpenes. The average particle size, Zeta potential, and stability were detected. The results of antitumor efficacy including tumor inhibitory rate, body weight change, immune organ index, and concentration of TNF-α and IL-6 were investigated. Besides, pathological section of tumor tissue and TUNEL labeling were conducted subsequently. Results: The prepared microemulsion displayed spherical surface with mean droplet size of (69.92 ± 8.43) nm, polydispersity (PDI) of 0.060 ± 0.008, and Zeta potential of (-11.30 ± 1.34) mV. Tumor inhibitory rate of microemulsion (57.25%) was significantly higher than that of suspension (45.89%), immune tissue index as well as the concentration of TNF-α and IL-6 were increased significantly. TUNEL labeling and pathological section of tumor tissue showed that the antitumor activity of microemulsion was significantly effective compared with that of suspensions. Conclusion: Lingyi Formula multicomponent microemulsion has a good anti-lung cancer activity.

Objective To evaluate the cancer risk of benzene and formaldehyde in different indoor air environments in Guiyang city.Methods From 2004 to 2005,benzene and formaldehyde monitoring was conducted in bedrooms,living rooms, kitchens,offices,classrooms and outdoor environments in winter,spring and autumn,and human health risk assessment was done. Results The mean benzene and formaldehyde concentration in different environments were lower than the China indoor air quality standard except for formaldehyde concentration in offices.The benzene cancer risks of male and female adults were 1.63?10~4 and 1.40?10~(-4)respectively.The formaldehyde cancer risks of male and female adults were 6.05?10~(-4)and 5.23?10~(-4) respectively.The human formaldehyde cancer risk was higher than benzene cancer risk,and the risk for male was higher than that for female.Benzene and formaldehyde cancer risks of different population in different indoor environments were above 1.00?10~(-6), the acceptable level of human cancer risk.Conclusion The benzene and formaldehyde concentrations in different environment in Guiyang city has the high cancer risks to human health.

OBJECTIVE To investigate the clinicopathological features of ossifying fibroma (OF) and fibrous dysplasia (FD) , and to evaluate the role of immunohistochemistry in differential diagnosis. METHODS Clinical presentation and morphological study of 40 cases with OF and 26 cases with FD were reviewed. Immunohistochemistry stains for PCNA, Ki-67, Laminin and CK 19 were performed in specimens of 10 cases with FD and 26 cases with OF. RESULTS The average age of OF was 22.2 years and the ratio of male and female was 2.33:1. The average age of FD was 17.2 years and the ratio of male and female is about1:1. The average age of the multibone type of FD was 11.2 years and the ratio of male and female is 1:2. Most of OF occurred in ethmoid sinus (75 %) and 70 % of the patients presented snuffle and hyposmia, while FD inclineed to arise from sphenoid (58 %) and 80.8 %of patients showed eyesight dropping and exophthalmos. The immunohistochemistry stains PCNA was positive in 19 cases (73.1 %) of OF and negative in 10 cases of FD. Ki-67, laminin, CK19 were of little significance. CONCLUSION The gender, age distribution, clinical and pathological features of OF and FD are different from each other. Immunohistochemistry staining for PCNA is of great value in their differential diagnosis.

Objective To explore the influence of smoking on olfactory disorder in Parkinson's disease (PD). Methods According to smoking or not, 167 PD patients ( PD group) and 100 normal controls ( normal control group) were divided into smoking subgroups and non-smoking subgroups.The olfactory identification threshold was tested by T&T olfactory assessment.Results Compared with normal control group, the scores of MMSE and montreal cognitive assessment in PD group were significantly decreased ( all P<0.05 ) , age, smoking history, the rate of male had no statistical significance ( all P>0.05 ) .The olfactory identification threshold in PD group was significantly higher than normal control group (t=6.785, P=0.000).Compared with PD smoking subgroup, the olfactory identification threshold in non-smoking subgroup was significantly higher (t=-3.000, P=0.003).The olfactory identification threshold in normal control smoking subgroup was significantly lower (t=0.784, P=0.435). The olfactory identification threshold of PD smokers had no correlation with smoking pack-years or duration ( r=-0.104, P=0.441;r=-0.156, P=0.246) .Conclusion Smoking may protect olfactory disorder in PD patients, and it has no correlation with smoking pack-years or duration.

Objective To explore the effects of extension nursing on quality of life in cervical cancer patients after radiotherapy. Methods A total of 80 hospitalization patients from January 2013 to January 2015 was selected to join the QQL-BREF test. These 80 patients were divided into the experimental group and the control group, who all accepted regular health education, and the experimental group also accepted nursing once a month after discharge. The quality of life of patients in the two groups were assessed one year later. Results For the domain of physiological field, psychological field, independence, social, environmental, faith, the experimental group scored 3.73 ± 0.33, 3.66 ± 0.44, 3.67 ± 0.05, 3.48 ± 0.59, 3.73 ± 0.58, while the control group scored 1.68 ± 0.40, 2.39 ± 0.43, 1.76 ± 0.39, 2.10 ± 0.51, 3.02 ± 0.45 (t=1.26-21.15, P < 0.05). There were 5 factors, expect faith factor, which conformed to statistical principle (P<0.05). In 24 factors, 7 elements including human characteristic, security, income, skills and personal religion were not related to the statistical principle (P>0.05), Other 17 elements showed statistical difference (t=2.45-17.31, P < 0.01). Conclusions Extension nursing can improve cervical cancer patients′quality of life efficiently after radiotherapy and reduce the recurrence rate.

Heart ; drug effects ; Heart Failure ; drug therapy ; Humans ; Hydrogen Bonding ; Nitrogen Oxides ; pharmacology ; therapeutic use