1.Bone Mineral Density and Osteoporosis in Men with Ankylosing Spondylitis
Tam Thi Minh Mai ; Tho Duc Tran ; Thuy Thi Thanh Vu
Journal of Medical Research 2008;0(1):108-112
Introduction: Ankylosing Spondylitis is a chronic rheumatic arthritis with specifically related to lumbar spine and femoral neck. The disease is more common in young men. Inflame and inactive condition of patients with Ankylosing Spondylitis leads to a low bone mineral density. Determining osteomalacia has clinical significance because of the relation to bone density.\r\n', u'Objectives: To study the prevalence of osteoporosis and osteopenia in Ankylosing Spondylitis and to investigate correlation between bone mineral density and age at the beginning of the disease, BASDAI, BMI\r\n', u'Subjects and methods:119 male patients with ankylosing spondylitis, mean age of 29.0 \xb1 10.8 as defined by New York criteria modified in 1984. Bone mineral density was measured at the lumbar spine and hip with Unigamma X ray - Plus. Results: 26.1 % of patients had lumbar spine osteopenia and osteoporosis, while 41.2% had femoral neck osteopenia and osteoporosis. The beginning of the disease was 22.1 \xb1 7.8 and had correlation with bone density in lumbar spine. Low BMI group had decreased bone density in lumbar spine and femoral neck (BMI < 18.5), (p=0.0001 and p=0,005, respectively). Patients with active disease had lower bone density than those with stable disease.\r\n', u'Conclusion: Ankylosing spondylitis patients have decreased BMD values at both the spine and femur. Bone mineral density at lumbar spine and femoral neck had correlation with the BMI, Bone mineral density at femoral neck had correlation with BASDAI. \r\n', u'
Ankylosing spondylitis
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Bone mineral density
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Osteoporosis
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DXA.
2.Anticancer effects of saponin and saponin-phospholipid complex of Panax notoginseng grown in Vietnam
Kim Dang Thu ; Thanh Nguyen Hai ; Thuy Nguyen Duong ; Duc Vu Loi ; Thi Vu Thu ; Manh Vu Hung ; Boonsiri Patcharee ; Thanh Bui Tung
Asian Pacific Journal of Tropical Biomedicine 2016;6(9):795-800
Objective: To evaluate the antitumor activity both in vitro and in vivo of saponin–phospholipid complex of Panax notoginseng. Methods: The in vitro cytotoxic effect of saponins extract and saponin–phospholipid complex against human lung cancer NCI-H460 and breast cancer cell lines BT474 was examined using MTS assay. For in vivo evaluation of antitumor potential, saponin and saponin–phospholipid complex were administered orally in rats induced mammary carcinogenesis by 7,12-dimethylbenz(a)anthracene, for 30 days. Results: Our data showed that saponin–phospholipid complex had stronger anticancer effect compared to saponin extract. The IC50 values of saponin–phospholipid complex and saponin extract for NCI-H460 cell lines were 28.47μg/mL and 47.97μg/mL, respectively and these values for BT474 cells were 53.18μg/mL and 86.24μg/mL, respectively. In vivo experiments, administration of saponin, saponin–phospholipid complex and paclitaxel (positive control) effectively suppressed 7,12-dimethylbenz(a) anthracene-induced breast cancer evidenced by a decrease in tumor volume, the reduction of lipid peroxidation level and increase in the body weight, and elevated the enzymatic antioxidant activities of su-peroxide dismutase, catalase, glutathione peroxidase in rat breast tissue. Conclusions: Our study suggests that saponin extract from Panax notoginseng and saponin–phospholipid complex have potential to prevent cancer, especially breast cancer.
3.The role of rapid tissue expansion in separating xipho-omphalopagus conjoined twins in Vietnam
Tran Thiet SON ; Pham Thi Viet DUNG ; Ta Thi Hong THUY ; Vu Duy KIEN ; Nguyen Thanh LIEM
Archives of Plastic Surgery 2021;48(4):378-383
Conjoined twins are rare, and each set of conjoined twins has a unique conjoined anatomy. It is necessary to perform separation to increase the chance of patient survival. Tissue expansion is an advanced technique for providing sufficient soft tissue and skin for wound closure. We report the successful application of rapid tissue expansion in 10-month-old xipho-omphalopagus conjoined twins in Vietnam. A tissue expander was placed on the anterior body between the sternum and umbilicus with a baseline of 70 mL sterile saline (0.9% NaCl). The first injection into the tissue expander began on the 6th day after expander insertion, and injections continued every 2 days with approximately 30–70 mL per injection according to the expansion of the skin. The expander reached 335 mL after six injections and within 10 days. In order to prepare for surgical separation, expansion was completed on the 15th day after insertion. The expanded skin area was estimated to be 180 cm2, which was sufficient to cover both patients’ skin deficiencies. The twins presented for surgical separation 6 days following the completion of tissue expansion. Both babies were discharged in good health 1 month after separation.
4.The role of rapid tissue expansion in separating xipho-omphalopagus conjoined twins in Vietnam
Tran Thiet SON ; Pham Thi Viet DUNG ; Ta Thi Hong THUY ; Vu Duy KIEN ; Nguyen Thanh LIEM
Archives of Plastic Surgery 2021;48(4):378-383
Conjoined twins are rare, and each set of conjoined twins has a unique conjoined anatomy. It is necessary to perform separation to increase the chance of patient survival. Tissue expansion is an advanced technique for providing sufficient soft tissue and skin for wound closure. We report the successful application of rapid tissue expansion in 10-month-old xipho-omphalopagus conjoined twins in Vietnam. A tissue expander was placed on the anterior body between the sternum and umbilicus with a baseline of 70 mL sterile saline (0.9% NaCl). The first injection into the tissue expander began on the 6th day after expander insertion, and injections continued every 2 days with approximately 30–70 mL per injection according to the expansion of the skin. The expander reached 335 mL after six injections and within 10 days. In order to prepare for surgical separation, expansion was completed on the 15th day after insertion. The expanded skin area was estimated to be 180 cm2, which was sufficient to cover both patients’ skin deficiencies. The twins presented for surgical separation 6 days following the completion of tissue expansion. Both babies were discharged in good health 1 month after separation.
5.Subacute thyroiditis after receiving the vaccine for COVID-19: a case report and literature review
Thanh Binh VU ; Dinh Toi CHU ; Dinh Tuan LE ; Thi Thuy Dieu HOANG ; Philippe GAUTRET ; Van Thuan HOANG
Clinical and Experimental Vaccine Research 2022;11(2):226-229
A 38-year-old female patient, with healthy history, was vaccinated with ChAdOx1 nCoV-19 (Astra Zeneca Cambridge, UK). Five days after the second injection, the patient presented headache, vertigo, then fatigue, nervousness, palpitations, shortness of breath, small amplitude tremors, and sweating episodes. Laboratory investigation revealed a suppressed thyroid-stimulating hormone (TSH), with elevated free thyroxine. However, the TSH receptor antibody and anti-thyroid peroxidase antibody were normal and thyroid-stimulating immunoglobulin negative. The patient was maintained on Metoprolol, and no specific treatment was added. After 3 months of following, the patient now feels comfortable. Our literature review found that 21 cases of subacute thyroiditis (SAT) following coronavirus disease 2019 (COVID-19) vaccines were reported. Most patients were young women who presented neck pain and systemic symptoms, with or without fever. These symptoms can appear as early (3 to 5 days), or later (1 month) after vaccination, regardless of vaccine type and mechanism of action. Laboratory tests showed decreased levels of TSH and elevated thyroid hormone. The mechanism of this event remains unknown. Further study is recommended to investigate the possible predisposing factors to developing SAT after receiving the COVID-19 vaccine.
6.An Outbreak of Trichinosis with Molecular Identification of Trichinella sp. in Vietnam.
Nguyen Van DE ; Nguyen Vu TRUNG ; Nguyen Hong HA ; Vu Thi NGA ; Nguyen Minh HA ; Pham Thanh THUY ; Le Van DUYET ; Jong Yil CHAI
The Korean Journal of Parasitology 2012;50(4):339-343
The 5th outbreak of trichinosis occurred in a mountainous area of North Vietnam in 2012, involving 24 patients among 27 people who consumed raw pork together. Six of these patients visited several hospitals in Hanoi for treatment. Similar clinical symptoms appeared in these patients within 5-8 days after eating infected raw pork, which consisted of fever, muscle pain, difficult moving, edema, difficult swallowing, and difficult breathing. ELISA revealed all (6/6) positive reactions against Trichinella spiralis antigen and all cases showed positive biopsy results for Trichinella sp. larvae in the muscle. The larvae detected in the patients were identified as T. spiralis (Vietnamese strain) by the molecular analysis of the mitochondrial cytochrome c oxidase subunit III (cox3) gene.
Adult
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Animals
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Antigens, Helminth/analysis/immunology
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*Disease Outbreaks
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Electron Transport Complex IV/genetics
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Enzyme-Linked Immunosorbent Assay
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Female
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Humans
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Larva
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Male
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Meat/*parasitology
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Mitochondria/genetics
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Muscles/parasitology/pathology
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Swine
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Trichinella spiralis/genetics/immunology/*isolation & purification
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Trichinellosis/*epidemiology/parasitology/pathology
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Vietnam/epidemiology
7.The potential efficacy of the E2-subunit vaccine to protect pigs against different genotypes of classical swine fever virus circulating in Vietnam
Ha Thi Thanh TRAN ; Duc Anh TRUONG ; Viet Duc LY ; Hao Thi VU ; Tuan Van HOANG ; Chinh Thi NGUYEN ; Nhu Thi CHU ; Vinh The NGUYEN ; Duyen Thuy NGUYEN ; Kohtaroh MIYAZAWA ; Takehiro KOKUHO ; Hoang Vu DANG
Clinical and Experimental Vaccine Research 2020;9(1):26-39
8.NecroX-5 exerts anti-inflammatory and anti-fibrotic effects via modulation of the TNFα/Dcn/TGFβ1/Smad2 pathway in hypoxia/reoxygenation-treated rat hearts.
Vu Thi THU ; Hyoung Kyu KIM ; Le Thanh LONG ; To Thanh THUY ; Nguyen Quang HUY ; Soon Ha KIM ; Nari KIM ; Kyung Soo KO ; Byoung Doo RHEE ; Jin HAN
The Korean Journal of Physiology and Pharmacology 2016;20(3):305-314
Inflammatory and fibrotic responses are accelerated during the reperfusion period, and excessive fibrosis and inflammation contribute to cardiac malfunction. NecroX compounds have been shown to protect the liver and heart from ischemia-reperfusion injury. The aim of this study was to further define the role and mechanism of action of NecroX-5 in regulating infl ammation and fi brosis responses in a model of hypoxia/reoxygenation (HR). We utilized HR-treated rat hearts and lipopolysaccharide (LPS)-treated H9C2 culture cells in the presence or absence of NecroX-5 (10 µmol/L) treatment as experimental models. Addition of NecroX-5 signifi cantly increased decorin (Dcn) expression levels in HR-treated hearts. In contrast, expression of transforming growth factor beta 1 (TGFβ1) and Smad2 phosphorylation (pSmad2) was strongly attenuated in NecroX-5-treated hearts. In addition, signifi cantly increased production of tumor necrosis factor alpha (TNFα), TGFβ1, and pSmad2, and markedly decreased Dcn expression levels, were observed in LPS-stimulated H9C2 cells. Interestingly, NecroX-5 supplementation effectively attenuated the increased expression levels of TNFα, TGFβ1, and pSmad2, as well as the decreased expression of Dcn. Thus, our data demonstrate potential antiinflammatory and anti-fibrotic effects of NecroX-5 against cardiac HR injuries via modulation of the TNFα/Dcn/TGFβ1/Smad2 pathway.
Animals
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Decorin
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Fibrosis
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Heart*
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Inflammation
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Liver
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Models, Theoretical
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Phosphorylation
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Rats*
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Reperfusion
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Reperfusion Injury
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Transforming Growth Factor beta
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Tumor Necrosis Factor-alpha
9.NecroX-5 protects mitochondrial oxidative phosphorylation capacity and preserves PGC1alpha expression levels during hypoxia/reoxygenation injury.
Vu Thi THU ; Hyoung Kyu KIM ; Le Thanh LONG ; Bayalagmaa NYAMAA ; In Sung SONG ; To Thanh THUY ; Nguyen Quang HUY ; Jubert MARQUEZ ; Soon Ha KIM ; Nari KIM ; Kyung Soo KO ; Byoung Doo RHEE ; Jin HAN
The Korean Journal of Physiology and Pharmacology 2016;20(2):201-211
Although the antioxidant and cardioprotective effects of NecroX-5 on various in vitro and in vivo models have been demonstrated, the action of this compound on the mitochondrial oxidative phosphorylation system remains unclear. Here we verify the role of NecroX-5 in protecting mitochondrial oxidative phosphorylation capacity during hypoxia-reoxygenation (HR). Necrox-5 treatment (10 microM) and non-treatment were employed on isolated rat hearts during hypoxia/reoxygenation treatment using an ex vivo Langendorff system. Proteomic analysis was performed using liquid chromatography-mass spectrometry (LC-MS) and non-labeling peptide count protein quantification. Real-time PCR, western blot, citrate synthases and mitochondrial complex activity assays were then performed to assess heart function. Treatment with NecroX-5 during hypoxia significantly preserved electron transport chain proteins involved in oxidative phosphorylation and metabolic functions. NecroX-5 also improved mitochondrial complex I, II, and V function. Additionally, markedly higher peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC1alpha) expression levels were observed in NecroX-5-treated rat hearts. These novel results provide convincing evidence for the role of NecroX-5 in protecting mitochondrial oxidative phosphorylation capacity and in preserving PGC1alpha during cardiac HR injuries.
Animals
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Anoxia
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Blotting, Western
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Citric Acid
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Electron Transport
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Heart
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Mitochondria
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Oxidative Phosphorylation*
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Peroxisomes
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Rats
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Real-Time Polymerase Chain Reaction
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Spectrum Analysis
10. Anticancer effects of saponin and saponin–phospholipid complex of Panax notoginseng grown in Vietnam
Thu DANG KIM ; Tung BUI THANH ; Hai NGUYEN THANH ; Duong NGUYEN THUY ; Loi VU DUC ; Thu VU THI ; Thu VU THI ; Hung VU MANH ; Patcharee BOONSIRI
Asian Pacific Journal of Tropical Biomedicine 2016;6(9):795-800
Objective To evaluate the antitumor activity both in vitro and in vivo of saponin–phospholipid complex of Panax notoginseng. Methods The in vitro cytotoxic effect of saponins extract and saponin–phospholipid complex against human lung cancer NCI-H460 and breast cancer cell lines BT474 was examined using MTS assay. For in vivo evaluation of antitumor potential, saponin and saponin–phospholipid complex were administered orally in rats induced mammary carcinogenesis by 7,12-dimethylbenz(a)anthracene, for 30 days. Results Our data showed that saponin–phospholipid complex had stronger anticancer effect compared to saponin extract. The IC50 values of saponin–phospholipid complex and saponin extract for NCI-H460 cell lines were 28.47 μg/mL and 47.97 μg/mL, respectively and these values for BT474 cells were 53.18 μg/mL and 86.24 μg/mL, respectively. In vivo experiments, administration of saponin, saponin–phospholipid complex and paclitaxel (positive control) effectively suppressed 7,12-dimethylbenz(a) anthracene-induced breast cancer evidenced by a decrease in tumor volume, the reduction of lipid peroxidation level and increase in the body weight, and elevated the enzymatic antioxidant activities of superoxide dismutase, catalase, glutathione peroxidase in rat breast tissue. Conclusions Our study suggests that saponin extract from Panax notoginseng and saponin–phospholipid complex have potential to prevent cancer, especially breast cancer.