1.Rapid detection of fungal keratitis by semi-nested PCR
Nhung Hong Pham ; Trung Vu Nguyen ; Ngoc Hong Le ; Lan Thi Thu Pham ; Thu Anh Tran
Journal of Medical Research 2008;56(4):46-50
Background: Fungal keratitis is a serious ocular infection that can cause corneal scarring and blindness. Currently, diagnosis of fungal pathogens remains a difficult problem. Objectives: To investigate the application of semi-nested PCR targeted ITS genes for detection of fungal agents causing keratitis. Material and method: Ten identified fungal strains, 4 bacterial strains, 20 scraping samples from patients with suspected fungal keratitis and 2 scraping samples from patients with suspected bacterial keratitis were tested using semi-nested PCR. Results: Semi-nested PCR showed positive results for the samples of identified fungal strains and for the 20 scraping samples from patients with suspected fungal keratitis. Neither samples of bacterial strains nor scraping samples from suspected bacterial keratitis patients gave positive PCR results. Conclusion: Semi-nested PCR is a robust tool for specific and rapid detection of fungal agents causing keratitis.
Fungal keratitis
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semi-nested PCR
2.Molecular and Clinicopathological Features of Gastrointestinal Stromal Tumors in Vietnamese Patients
Quoc Dat NGO ; Quoc Thang PHAM ; Dang Anh Thu PHAN ; Anh Vu HOANG ; Thi Ngoc Ha HUA ; Sao Trung NGUYEN
Journal of Pathology and Translational Medicine 2019;53(6):361-368
BACKGROUND: Gastrointestinal stromal tumors (GISTs) are the most frequent mesenchymal neoplasms of the gastrointestinal tract. Management of GIST patients is currently based on clinicopathological features and associated genetic changes. However, the detailed characteristics and molecular genetic features of GISTs have not yet been described in the Vietnamese population.METHODS: We first identified 155 patients with primary GIST who underwent surgery with primary curative intent between 2011 and 2014 at University Medical Center at Ho Chi Minh City, Vietnam. We evaluated the clinicopathological features and immunohistochemical reactivity to p53 and Ki-67 in these patients. Additionally, KIT genotyping was performed in 100 cases.RESULTS: The largest proportion of GISTs was classified as high-risk (43.2%). Of the 155 GISTs, 52 (33.5%) were positive for Ki-67, and 58 (37.4%) were positive for p53. The expression of Ki-67 and p53 were correlated with mitotic rate, tumor size, risk assessment, and tumor stage. Out of 100 GIST cases, KIT mutation was found in 68%, of which 62 (91.2%) were found in exon 11, two (2.9%) in exon 9, and four (5.8%) in exon 17. No mutation in exon 13 was identified. Additionally, KIT mutations did not correlate with any clinicopathological features.CONCLUSIONS: The expression of Ki-67 and p53 were associated with high-risk tumors. Mutations in exon 11 were the most commonly found, followed by exon 17 and exon 9. Additionally, KIT mutation status was not correlated with any recognized clinicopathological features.
Academic Medical Centers
;
Asian Continental Ancestry Group
;
Exons
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Gastrointestinal Stromal Tumors
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Gastrointestinal Tract
;
Humans
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Molecular Biology
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Risk Assessment
;
Vietnam
3.Study on the production of measles antibody used for vaccine quality control
Thu Nu Anh Nguyen ; Huong Thu Ngo ; Thu Anh Pham ; Dung Trung Le ; Nga Thi Nguyen ; Hien Dang Nguyen ; Luan Thi Le ; Thanh Kim Dang ; Thanh Kim Dang ; Thanh Kim Dang ; Thanh Kim Dang ; Thai Minh Ngo ; Thai Minh Ngo ; Thai Minh Ngo ; Thai Minh Ngo
Journal of Preventive Medicine 2008;18(6):74-78
Background: With the help of Japan, the Center for Research and Production of vaccines and biologicals, Hanoi has received a WHO standard measles vaccine production technology, including techniques in the examination of vaccine quality. Therefore, it is needed to be initiative on production of measles antibody. Objective: Study on production of measles antibody in rabbits and selecting the appropriate antibody for production of high titre antibody, which meets the standard of vaccine quality control in Vietnam. Subject and methods: Using the measles antigen from Edmonston and AIK-C strains, which were provided by the Kitasato Institute, to produce measles antibody. Making immunoreaction in rabbits and determination of neutralization antibody titre. Results and Conclusion: Measles antigen of Edmonston Vero 7/P2 strain used in the production of measles antibody in rabbit created the highest antibody titre in comparison with AIK-C strain from vero cell and FL cell supplied by the Kitasato Institute of Japan. Antibody titre of Edmonston Vero 7/P2 strain reached up to 1/8192 and met the sera standard required for measles vaccine quality control, it is similar to the measles sera to be produced from the Kitasato Institute.
measles antibody
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antibody titre
4.Predictors of twin pregnancy in in vitro fertilization with intracytoplasmic sperm injection cycles with day 3 double embryo transfer
Duy Le NGUYEN ; Hieu Le-Trung HOANG ; Vu Ngoc-Anh HO ; Toan Duong PHAM ; Nam Thanh NGUYEN ; Van Thi-Thu TRAN ; Tuong Manh HO ; Lan Ngoc VUONG
Clinical and Experimental Reproductive Medicine 2024;51(1):69-74
Objective:
The purpose of this study was to identify factors associated with twin pregnancy following day 3 double embryo transfer (DET).
Methods:
This retrospective cohort study incorporated data from 16,972 day 3 DET cycles. The participants were women aged between 18 and 45 years who underwent in vitro fertilization with intracytoplasmic sperm injection (IVF/ICSI) at My Duc Assisted Reproduction Technique Unit (IVFMD), My Duc Hospital, located in Ho Chi Minh City, Vietnam.
Results:
Of the 16,972 day 3 DET cycles investigated, 8,812 (51.9%) resulted in pregnancy. Of these, 6,108 cycles led to clinical pregnancy, with 1,543 (25.3% of clinical pregnancies) being twin pregnancies. Factors associated with twin pregnancy included age under 35 years (odds ratio [OR], 1.5; 95% confidence interval [CI], 1.32 to 1.71; p<0.001) and cycles involving the transfer of at least one grade I embryo. Relative to the transfer of two grade III embryos, the risk of twin pregnancy was significantly elevated following the transfer of two grade I embryos (OR, 1.40; 95% CI, 1.16 to 1.69; p<0.001) or a combination of one grade I and one grade II embryo (OR, 1.27; 95% CI, 1.05 to 1.55; p=0.001).
Conclusion
By analyzing a large number of IVF/ICSI cycles, we identified several predictors of twin pregnancy. These findings can assist medical professionals in tailoring treatment strategies for couples with infertility.
5.Identification of Enterovirus C105 for the first time in New Zealand
Cong Thanh Duong ; Tran Hien Nguyen ; Anh Tuan Nguyen ; Thi Thanh Ha Hoang ; Hong Thang Pham ; Thi THanh Ha Nguyen ; Anh Tuan Le ; Dai Quang Tran ; Hong Tram Tran ; Le Hai Nguyen ; Thi Thu Huong Phan ; Hai Son Vo ; Hoang Duc Bui ; Thien Nga Nguyen ; David Jacka ; Keith Sabin
Western Pacific Surveillance and Response 2015;6(1):52-54
In this report we describe a new approach in HIV sentinel surveillance that was piloted in Viet Nam in 2009 and is currently being rolled out in all provinces. It comprises a brief behavioural questionnaire added to the HIV sentinel surveillance surveys conducted routinely among people who inject drugs, female sex workers and men who have sex with men. Timely reporting of data from this system has resulted in improvements to HIV prevention efforts for most at-risk populations.
6.Identification of Soluble Epoxide Hydrolase Inhibitors from the Seeds of Passiflora edulis Cultivated in Vietnam
To Dao CUONG ; Hoang THI NGOC ANH ; Tran Thu HUONG ; Pham Ngoc KHANH ; Vu Thi HA ; Tran Manh HUNG ; Young Ho KIM ; Nguyen Manh CUONG
Natural Product Sciences 2019;25(4):348-353
Soluble epoxide hydrolases (sEH) are enzymes present in all living organisms, metabolize epoxy fatty acids to 1,2-diols. sEH in the metabolism of polyunsaturated fatty acids plays a key role in inflammation. In addition, the endogenous lipid mediators in cardiovascular disease are also broken down to diols by the action of sEH that enhanced cardiovascular protection. In this study, sEH inhibitory guided fractionation led to the isolation of five phenolic compounds trans-resveratrol (1), trans-piceatannol (2), sulfuretin (3), (+)-balanophonin (4), and cassigarol E (5) from the ethanol extract of the seeds of Passiflora edulis Sims cultivated in Vietnam. The chemical structures of isolated compounds were determined by the interpretation of NMR spectral data, mass spectra, and comparison with data from the literature. The soluble epoxide hydrolase (sEH) inhibitory activity of isolated compounds was evaluated. Among them, trans-piceatannol (2) showed the most potent inhibitory activity on sEH with an IC₅₀ value of 3.4 µM. This study marks the first time that sulfuretin (3) was isolated from Passiflora edulis as well as (+)-balanophonin (4), and cassigarol E (5) were isolated from Passiflora genus.
Cardiovascular Diseases
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Epoxide Hydrolases
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Ethanol
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Fatty Acids
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Fatty Acids, Unsaturated
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Inflammation
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Metabolism
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Passiflora
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Passifloraceae
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Phenol
;
Vietnam
7.Virological characteristics of cases of COVID-19 in northern Viet Nam, January–May 2020
Hang Khanh Le Nguyen ; Son Vu Nguyen ; Phuong Mai Vu Hoang ; Thanh Thi Le ; Huong thi Thu Tran ; Long Hai Pham Nguyen ; Thai Quang Pham ; Thuy Thanh Nguyen ; Anh Duc Dang ; Anh Phuong Nguyen ; Mai thi Quynh Le
Western Pacific Surveillance and Response 2021;12(4):65-70
Background:
Viet Nam confirmed its first case of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection on 23 January 2020 among travellers from Wuhan, China, and experienced several clusters of community transmission until September. Viet Nam implemented an aggressive testing, isolation, contact tracing and quarantine strategy in response to all laboratory-confirmed cases. We report the results of SARS-CoV-2 testing during the first half of 2020 in northern Viet Nam.
Methods:
Between January and May 2020, 15 650 upper respiratory tract specimens were collected from 14 470 suspected cases and contacts in northern Viet Nam. All were tested for SARS-CoV-2 by real-time RT-PCR. Individuals with positive specimens were tested every three days until two tests were negative. Positive specimens from 81 individuals were cultured.
Results:
Among 14 470 tested individuals, 158 (1.1%) cases of SARS-CoV-2 infection were confirmed; 89 were imported and 69 were associated with community transmission. Most patients (122, 77%) had negative results after two tests, while 11 and 4 still tested positive when sampled a third and fourth time, respectively. SARS-CoV-2 was isolated from 29 of 81 specimens (36%) with a cycle threshold (Ct) value <30. Seven patients who tested positive again after testing negative had Ct values >30 and negative cultures.
Conclusion
Early, widespread testing for SARS-CoV-2 in northern Viet Nam identified very few cases, which, when combined with other aggressive strategies, may have dramatically contained the epidemic. We observed rapid viral clearance and very few positive results after clearance. Large-scale molecular diagnostic testing is a critical part of early detection and containment of COVID-19 in Viet Nam and will remain necessary until vaccination is widely implemented.
8.Impact of long COVID-19 on posttraumatic stress disorderas modified by health literacy: an observational study inVietnam
Han Thi VO ; Tien Duc DAO ; Tuyen Van DUONG ; Tan Thanh NGUYEN ; Binh Nhu DO ; Tinh Xuan DO ; Khue Minh PHAM ; Vinh Hai VU ; Linh Van PHAM ; Lien Thi Hong NGUYEN ; Lan Thi Huong LE ; Hoang Cong NGUYEN ; Nga Hoang DANG ; Trung Huu NGUYEN ; Anh The NGUYEN ; Hoan Van NGUYEN ; Phuoc Ba NGUYEN ; Hoai Thi Thanh NGUYEN ; Thu Thi Minh PHAM ; Thuy Thi LE ; Thao Thi Phuong NGUYEN ; Cuong Quoc TRAN ; Kien Trung NGUYEN
Osong Public Health and Research Perspectives 2024;15(1):33-44
Objectives:
The prevalence of posttraumatic stress disorder (PTSD) has increased, particularly among individuals who have recovered from coronavirus disease 2019 (COVID-19) infection. Health literacy is considered a “social vaccine” that helps people respond effectively to the pandemic. We aimed to investigate the association between long COVID-19 and PTSD, and to examine the modifying role of health literacy in this association.
Methods:
A cross-sectional study was conducted at 18 hospitals and health centers in Vietnamfrom December 2021 to October 2022. We recruited 4,463 individuals who had recovered from COVID-19 infection for at least 4 weeks. Participants provided information about their sociodemographics, clinical parameters, health-related behaviors, health literacy (usingthe 12-item short-form health literacy scale), long COVID-19 symptoms and PTSD (Impact Event Scale-Revised score of 33 or higher). Logistic regression models were used to examine associations and interactions.
Results:
Out of the study sample, 55.9% had long COVID-19 symptoms, and 49.6% had PTSD.Individuals with long COVID-19 symptoms had a higher likelihood of PTSD (odds ratio [OR], 1.86; 95% confidence interval [CI], 1.63–2.12; p < 0.001). Higher health literacy was associated with a lower likelihood of PTSD (OR, 0.98; 95% CI, 0.97–0.99; p = 0.001). Compared to those without long COVID-19 symptoms and the lowest health literacy score, those with long COVID-19 symptoms and a 1-point health literacy increment had a 3% lower likelihood of PTSD (OR, 0.97; 95% CI, 0.96–0.99; p = 0.001).
Conclusion
Health literacy was found to be a protective factor against PTSD and modified the negative impact of long COVID-19 symptoms on PTSD.
9. Ethanol extracts of Scutellaria baicalensis protect against lipopolysaccharideinduced acute liver injury in mice
Hai Nguyen THANH ; Tuan Anh LE ; Huong Duong Thi LY ; Tung Nguyen HUU ; Loi Vu DUC ; Thu Dang KIM ; Tung Bui THANH ; Hue Pham Thi MINH
Asian Pacific Journal of Tropical Biomedicine 2015;5(9):761-767
Objective: To investigated the protective potential of ethanol extracts of Scutellaria baicalensis (S. baicalensis) against lipopolysaccharide (LPS)-induced liver injury. Methods: Dried roots of S. baicalensis were extracted with ethanol and concentrated to yield a dry residue. Mice were administered 200 mg/kg of the ethanol extracts orally once daily for one week. Animals were subsequently administered a single dose of LPS (5 mg/kg of body weight, intraperitoneal injection). Both protein and mRNA levels of cytokines, such as tumor necrosis factor alpha, interleukin-1β, and interleukin-6 in liver tissues were evaluated by ELISA assay and quantitative PCR. Cyclooxygenase-2, inducible nitric oxide synthase, and nuclear factor-kB protein levels in liver tissues were analyzed by western blotting. Results: Liver injury induced by LPS significantly increased necrosis factor alpha, interleukin-1β, interleukin-6, cyclooxygenase-2, inducible nitric oxide synthase, and nuclear factor-κB in liver tissues. Treatment with ethanol extracts of S. baicalensis prevented all of these observed changes associated with LPS-induced injury in liver mice. Conclusions: Our study showed that S. baicalensis is potentially protective against LPSinduced liver injury in mice.
10.Isolation and toxicity of Xanthomonas oryzae pv. oryzae in rice and a collection of phages against the pathogen
Ngoc Quynh Anh Pham ; Vo Thanh Phuc ; Pham Do Tra My ; Le Thi My Duyen ; To Hue Ngoc ; Nguyen Minh Thien ; Nguyen Hai Duong ; Le Thi Thuy Tien ; Le Phi Nga ; Hoang Anh Hoang
Malaysian Journal of Microbiology 2024;20(no.1):15-23
Aims:
The objective of the study was to isolate bacteriophages and conduct a comprehensive analysis of their potential against Xanthomonas oryzae pv. oryzae (Xoo) strains in the Mekong Delta, Vietnam.
Methodology and results:
Twelve Xoo strains were isolated from rice fields located in the Mekong Delta, Vietnam. Among these strains, three strains Xoo L019, L020 and L024, showed the highest disease index of bacterial blight. Four
phages specific to Xoo were isolated from soil, water and leaf samples, and their morphologies were determined. In a test against 12 Xoo strains, phage L541, MLA23 or W41 could infect 10 of the 12 Xoo strains, while phage LBH01 could infect 8 of the 12 Xoo strains. The stability of the phages to pH, organic solvents, UV-A and UV-B was also evaluated.
Conclusion, significance and impact of study
The initial characterization of the phages indicates their potential as biocontrol agents against bacterial blight in rice. The study is one of the very first studies about Xoo phages in rice in Vietnam.