Pla sequence composes of 480 nucleodides of pPCP1 plasmide originated from Yersinia pestis isolated from Viet Nam and collected by the technique of PCR of chemical line into TA vector and demonstrated in turn. PCR reaction gave specific result from 3 diversified sources for mould: total extracted ADN, ADN extracted from plasmide isolated from bacterium complete cell. A fast and accurate method of diagnosis was created basing on this operation. By BLAST programme for accessing Gene Bank, the pla-gene sequence of Yersinia pestis in Viet Nam is analogue to other strains worldwide
Yersinia pestis ; Genetic Markers ; Biochemistry

Using polymerase chain reaction (PCR) and sequencing, 492 nucleotides of pra gene was obtained from clinical swabs of the Vietnamese patients for comparative analysis with the corresponding sequences of the global strains of Mecobacterium leprae. As entry data for searching in Genbank, the Vietnamese strains of M.leprae showed very high homology level to the other global strains. In comparision to the standard TN strain, absolute homology (100%) for QH1 (designated as MLVN1) and 98-99% homology for QH2 strain (designated as MLVN2) of the Vietnamese M. leprae was observrd. There may be a number of variants among the M. leprae population in Vietnam. For final conclusion, thus, investigation must be done for many other isolates of patients and geographic origins. This is the first molecular-based identification for M. leprae in Vietnam. Our results afford establishing diagnostic and identification methodologies/techniques for M. leprae from clinical swabs from patients in Vietnam
Mycobacterium leprae ; Molecular Biology ; Polymerase Chain Reaction

By the method of capillary electrophresis with MEKC technique on the preparation without processing of samples such as celebrex, zycel. The procedure of quantifisation of celebroxib was established. Statintrics results found that after 6 times of electrophresis the Corr Area ratio between celebroxib and our standards gave RSD ≤ 3% and t/t is ≤ 2%. Thus the procedure altained systematic compatibility for quantifying the preparation. The method had high sensitivity and selectivity. The results was precis and repeatable
Pharmaceutical Preparations/administration & dosage ; Methods ; Electrophoresis

The total sequence of 18S rRNA and the neighbours including 1950 pairs of nucleotide (N) was received by PCR and expressed orderly. The examinated sample was 1 of 8 adult worms collected from 12.5 years old male patient. The worm was determined morphologically as Fasciolopsis buski. The comparison showed that in this intestinal fluke worm there is an almost absolutely analogous coefficient concerning nitrogen components of 18S rRNA in only 2 differences on 1950 N (0.01%) with the gene sequence of 18S rRNA kept in the bank of genes. In Viet Nam, this is the first molecular determination realized on human
Fasciolidae ; Trematoda ; RNA, Ribosomal, 18S

Clinical characteristics and cerebrospinal fluid abnormalities in 100 cases of confirmed B type of H.influenzae (Hib) meningititis were analysed in comparing with 105 cases of meningitis caused by other bacterial pathogens. The basic criteria for early diagnosis of Hib meningitis in under five year old children were found out. Results showed that 48.8% of cases of meningititis were due to Hib; 96% of CSF samples due to Hib had physiological change in comparing with 72.4% in meningitis due to other bacterial pathogens, in 92% there were clinical manifestations and biochemical/cytological typical changes in CSF, in 100% of cases of Hib meningitis(+), there were typical changes; 51% of cases there were positive blood culture and 47% there were Hib positive CSF and blood culture concurrently
Meningitis ; Child ; Haemophilus influenzae

Seventy-nice pediatric patient - family pairs were detected for Hib or Hib-antigen. The study also examined 22 pairs of H. influenzae strains isolated from CSF of patients and from upper respiratory tract of the family members of the patients to determine serotype, biotype, presence of Beta-lactamase, PCR with Hib-specified primers, and PFGE with SmaI restriction enzyme. The results showed that 44.4% of the patients` families had Hib carriers; all of 22 H. influenzae isolated pairs from patients and their families had serotype b; 17 of 22 pairs had characteristics of biotype II; 16 of 22 pairs had Beta-lactamase; and 9 of 22 pairs had both the PCR pattern and PFGE pattern. Close contact with Hib carriers was a factor of Hib meningitis contagiousness in children under 5 years of age
Family ; Haemophilus influenzae type b ; Meningitis ; child

Study on total 3rd grade pupils at 5 primary schools in Bac Can province in school year 2001-2002. Results: the rate of dental carries of primary teeth was 64%, of permanent teeth was 12.3%, the decayed, missing and filled teeth (DMFT) index was 2.1 in primary teeth and 0.18 in permanent teeth. There were 99.13% of water samples with low concentration of fluor, among them 98.26% of samples was lower than 0.5mg/L. All these rates and indexes were higher than national rates and indexes.
Dental Caries ; Water ; Pupil

Study on the trend of chronic renal failure treatment with traditional Chinese medicine in the recent years. Results: in clinical settings, serum urea and creatinine were decreased; renal blood supply was improved; metabolisms of serum lipid, phosphorus and calcium were improved; body immune was increased, and the quality of life was improved, thence the progression of disease was slowly. However, there were some problems with this treatment and it is needed further research.
Kidney Failure, Chronic ; Therapeutics ; Medicine, Chinese Traditional

Study on 408 gestational diabetes women (mean age: 28.6± 0.56 years old) admitted Nam Dinh Obstetrics and Gynecology Hospital between June and December 2004. Results: incidence of glucose tolerance disorder and gestational diabetes in labouring women was 2.94%. 13 participants were diagnosed diabetes (3.18%), 48.04% participants were workers, 96.24% participants were in the 1st and 2nd pregnancies. 69.23% of gestational diabetes women had labour lasted for more than 24 hours. The rates of fetal distress and C-section in gestational diabetic group were higher than normal group (46.15% and 38.46% versus 32.91% and 31.90%, respectively). The incidence of puerperium infections was higher than normal group (7.7% versus 0.76%), and incidence of babies with congenital malformation also higher (15.38% versus 0.25%).
Glucose Intolerance ; Women ; Epidemiology

Studies of 805 samples until 10/2004 show that the malaria prevalence was high in the first 5 months in 2003, among 7 deaths were unorganized migrants. They contract malaria all year round, especially in the beginning of the year with the highest of 11.96% in February. The density of maralia parasite (+) was high in months at the beginning and end of the year such as January, April and October with the density in the first month of 2.92%. Spleenomegaly was mostly seen in age 5-14 with 3.17% and mostly in grade I and II (97.8%). Spleenomegaly grade III and IV is predominated in over 14 years old group (60%). Vectors at the first three months of the year were high and various in species in accordance with the malaria prevalence. An. dirus and An. minimus as the two main vectors of transmitting were present in almost of the year both house inside and outside, which is the cause for the transmission
Malaria ; Epidemiology