OBJECTIVE To study the dynamic changes of drug resistance and infectious distribution of Pseudomonas aeruginosa and guide drug treatment in clinic.METHODS The susceptibility to 12 antibiotics were detected in 758 P.aeruginosa strains.RESULTS The resistance of P.aeruginosa to 12 antibiotics was increased in the past four years.The resistant rate to imipenem and amikacin was below 25%.The infection caused by P.aeruginosa happened frequently in Department for Cadre,Intensive Care Units(ICU) and Department of Respiration.Among 758 strains of P.aeruginosa,87 strains were identified multi-drug resistant and found more frequently in Department for Cadre,ICU,Department of Respiration and Department of Burn.CONCLUSIONS The drug resistance of P.aeruginosa has become a more serious problem than before.The monitor of drug-resistant P.aeruginosa could guide treatment and provided epidemiologic and dynamic changes data for clinic.The infection of multi-drug resistant P.aeruginosa meets most frequently in patients who received antibiotic treatment for a long time and had a long stay in the hospital.