Pulse diagnosis has been taking an important part, together with the other diagnostic techniques, for determination of symptoms and treatment of patients with acupuncture. However, the traditional expression of pulse is complicated and subjective, so that the understanding of pulse diagnosis in termes of modern medicine is almost impossible. We tried to objectify the expression of pulse wave.
The pulse wave recorded from the six positions on radial artery according to the method described in Chinese Medicine through a force transducer. The recordings were processed for Fourier analysis utilizing a computer system. Characteristics of the pulse wave can be expressed quantitatively as a form of power spectrum of its components. The preliminary results of this study was reported at the 31th annual meeting of the Japan Society of Acupuncture. This time, we report the analysis on the basic pulse waves which have been recognized as negative-positive and imaginaryreal pulses.
Method
The subjects were normal volunteers of 36 males and 17. females. A force tranducer (Nihon Kohden TF-11S) Equipped with 0.52cm² of sensitive area was placed on the radial artery under the pressure ranging from 20 to 550gr. The pulse wave thus recorded was led into the electronic computer (HITAC-20) to obtain the power spectrum of the frequencies.
Results
It was found that characteristics of the six kinds of pulse waves, i. e., floating-sunken, smooth-leap and slow-fast types, can be represented quantitatively in terms of frequency histogram. Further, a possibility to express so called "complex pulse" in Chinese Medicine by this method is suggested.

We investgated the effect of electric acupuncture on peripheral T lymphocyte subset from normal individuals, analysed by laser flow cytometric system with monoclonal antibodies for human T lymphocyte subset.
OKT3⁺ cells (peripheral T cells) and OKT4⁺ cells (helper/inducer cells) did not show any changes after electric acupuncture. OKT8⁺ cells (suprressor/cytotoxic T cells), however, OKT11⁺ cells (E rosette positive T cells) and Leu7⁺ cells (natural killer cells) increased and Leu11⁺ cells (another population of natural killer cells) decreased after electric acupuncture.
These changes of peripheral T lymphocyte subsets would suggest that electric acupuncture effects human immune system.

In our clinic, blood pressure measurement has been done for many patients with various chief complaints. Acupuncture treatment was undertaken to improve their chief complaints according to Kurono's whole-body adjustment. We carried out a research paying attention to the fact that the patients with elevations of the blood pressure at the first visit showed a tendency to gradual decrease of the values.
According to the values of the first measurement, patients were divided into two groups in accordance with WHO's criteria: Hypertension group and Boundary one.
The first measurement velues and the averaged values of the following seven measurements undertaken before treatment were compared using histgram and t-test.
The descent of the blood pressure was seen in both Hypertension and Boundary group.

Recently it is well known that human immune system is regulated by various other biological response systems; endocrine and nervous system. We have investigated the effect of electric acupuncture on human immune system for several years. Those studies suggest that electric acupuncture affects human immune system via neuro-immune system. In the present study we investigated the effect of endorphin on human peripheral lymphocytes.
At the present concentration of endorphin and naloxone there were no effect on viability of lymphocytes. ³H-thymidine up take of unstimulated lymphocytes were suppressed by a appropriate concentration of endorphin or naloxone. The mitogenic responsiveness of peripheral lymphocytes was suppressed at usual concentration of naloxone and higher concentration of endorphin.
In these data suggested that both of intrisic and extrinsic endorphin regulated human lymphocyte responsiveness.