Recently it is well known that human immune system is regulated by various other biological response systems; endocrine and nervous system. We have investigated the effect of electric acupuncture on human immune system for several years. Those studies suggest that electric acupuncture affects human immune system via neuro-immune system. In the present study we investigated the effect of endorphin on human peripheral lymphocytes.
At the present concentration of endorphin and naloxone there were no effect on viability of lymphocytes. ³H-thymidine up take of unstimulated lymphocytes were suppressed by a appropriate concentration of endorphin or naloxone. The mitogenic responsiveness of peripheral lymphocytes was suppressed at usual concentration of naloxone and higher concentration of endorphin.
In these data suggested that both of intrisic and extrinsic endorphin regulated human lymphocyte responsiveness.

In recent years there is much talk of prevention or early detection of diseases. The number of persons undergoing complete general testing and physical examinations has increased. However among the patients coming to the acupuncture clinic are persons who upon undergoing physical examinations at hospitals were told results showed nothing wrong and yet they continue to experimence subjective or non-objective symptoms. Concerned with this problem I checked the distribution of patients coming to the clinic.
1, 838 persons from among the 2, 297 new patients who visited our clinic during the 5 years, 2-months from Oct. 1, 1973 to Dec. 30, 1980 who had been diagnosed by a physician were used as subjects of this examination. Results indicated that of these 1, 838 persons 258 or in other words, 14% of the subjects were experiencing subjective or non-objective symptoms.
I reported on the 1, 649 patients who visited the clinic in 1974, however, as together with this discussion some interesting results concerning the patient distribution of non-“subjective and non-objective symptom” patients were obtained, I'd like to also report on these.

Pulse diagnosis is one of the most important diagnostic method in Chinese medicine, however, the expression of this diagnostic technique is subjective and cannot be in this form compared with modern medicine. Many attempts to describe the pulse conditions objectively have been made, however, there has been little success.
Any wave can be described by the frequency distribution of the energy, according to Fourier. Using this method, we tried to objectify the pulse wave. For the recording of the pulse, a pick-up (Nihon Kohden TF-11S) was fixed in a place at the radial artery and pressure applied using a micromanipulator. The output was put into a data recorder through an amplifier and monitored by an oscillograph. Data was then processed using computer (HITAC-20) and power spectrums were displayed.
In the above described method, the minimum and maximum force which should be applied to detect the pulse wave varied from person to person. The range was from 10-50g and 400-600g for minimum and maximum, respectively. This can be considered as individual variations of so called “floating and sunken” pulses. The best shape of pulse waves were obtained with 100-300g of pressure.
A typical power spectrum of pulse wave was composed of standard sine curve (approximately 1Hz, basic wave) and its harmonics. Sharply peaked waves occured when the basic component was great and oppositely, the flatter waves contained abundant of harmonics. The ratio of the basic wave to its harmonics represents the characteristics of wave pattern, i. e., objective description of pulse conditions can be made.
We will then, investigate the correspondence of the recordings to the expressions in the Chinese medicine, influences of acupuncture, etc.