1.Study on gene mutations of single S region in immunoglobulin class switch recombination
Shenghe TIAN ; Kazuo KINOSHITA ; Tasuku HONJO
Chinese Journal of Immunology 2000;0(11):-
Objective:To investigate the gene rearrangements in immunoglobulin single switch(S) region during class switch recombination.Methods:An artificial substrate containing single S region(FS?-2) driven by tetracycline-responsive promoter was constructed and underwent CSR in a switch-inducible B lymphoma line was stimulated by cytokines,then the S region mutations and its dependent factors were detected by detected PCR and sequencing.Results:Gene rearrangements including deletions,insertions as well as point mutations could occur even in this single S region after cytokines stimulation;Furthermore,when the upstream promoter was suppressed by its inhibitor,CSR frequency was significantly decreased,indicating a transcription dependent S region recombination.Conclusion:Class switch recombination mechanism can exert a similar effect on single S region.
2.The Establishment of IL - 2 Dependent Cell Line from IL - 3 Dependent Mouse Hemopoietic Progenitor Cell Line and Its Analysis.
Kwang Ho LEE ; Chang Kwon KO ; Min Dong SONG ; Tasuku HONJO
Korean Journal of Immunology 1997;19(1):39-48
No abstract available.
Animals
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Cell Line*
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Mice*
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Polymerase Chain Reaction
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Stem Cells*
3.Career Choice.
Daizo USHIBA ; Shigekoto KAIHARA ; Taiichi SAITO ; Tsuyoshi SHIMIZU ; Rintaro NAKAYA ; Kazumasa HOSHINO ; Tasuku HONJO ; Wataru MORI
Medical Education 1986;17(1):8-15
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