1.The Anti-apoptosis Effect of GM1Ganglioside on Nerve Cell after the Spinal Cord Injury
Tao CHEN ; Peiyuan XIA ; Jianhong CHEN ; Haichuan MU
China Pharmacy 2001;0(12):-
OBJECTIVE:To investigate the anti-apoptosis effect and the mechanism of GM 1 ganglioside on nerve cell af?ter the spinal cord injury.METHODS:The rats with compressed injury at the T8.9level of spinal cord were employed as the model.Then the rats were divided into two groups at random,one for the control and one for GMI treatment.The rats in each group were administrated normal saline solution(20?l)and GM 1 (30?g,20?l)intrathecally10minutes after injury,respective?ly.The apoptosis of nerve cells in the injured spinal cord were examined by TUNEL and flow cytometry with nerve cells labeled with Annexin V/PI;and Caspase3activity was measured by fluorometric immunosorbent enzyme assay.RESULTS:The apoptotic cells appeared in both groups at4h and reached their peak on3rd day after the injury.The proportion of apoptotic cells and the intracelluar Caspase3activity in the GM 1 treated group were significant lower than those in the control group(P
2.Preparation and Clinical Observation of Sterilized Medical Bone Wax
Tao GUO ; Hongsheng MA ; Ping MU ; Shengchuan GAO ; Yan MA ; Dasheng DANG ; Tao LIU ; Chaohong CAI ; Weijie XIA
China Pharmacy 1991;0(05):-
OBJECTIVE:To prepare the sterilized medical bone wax and to establish the standard of quality control.METHODS:The bone wax was identified with chemical approach and the quality of bone wax was evaluated by saponification value.RESULTS:The bone wax was appropriate in formula,feasible in preparing technique and satisfactory in therapeutic efficacy with a satisfication rate of 98%.CONCLUSION:There are no obvious differences between the bone wax developed by our hospital and imported bone wax in quality,therefore the prepared bone wax can take the place of imported products.
3.Increased pretreatment levels of serum LDH and ALP as poor prognostic factors for nasopharyngeal carcinoma.
Guo LI ; Jin GAO ; Ya-Lan TAO ; Bing-Qing XU ; Zi-Wei TU ; Zhi-Gang LIU ; Mu-Sheng ZENG ; Yun-Fei XIA
Chinese Journal of Cancer 2012;31(4):197-206
Serum enzymes that play potential roles in tumor growth have recently been reported to have prognostic relevance in a diverse array of tumors. However, prognosis-related serum enzymes are rarely reported for nasopharyngeal carcinoma(NPC). To clarify whether the level of serum enzymes is linked to the prognosis of NPC, we reviewed the pretreatment data of lactate dehydrogenase(LDH), alkaline phosphatase (ALP), and glutamyl transferase (GGT) in 533 newly diagnosed NPC patients who underwent radical radiotherapy between May 2002 and October 2003 at Sun Yat-sen University Cancer Center. Patients were grouped according to the upper limit of normal values of LDH, ALP, and GGT. The Kaplan-Meier method and log-rank test were used for selecting prognostic factors from clinical characteristics and serum enzymes, and the chi-square test was applied to analyze the relationships of clinical characteristics and serum enzymes. Finally, a Cox proportional hazards model was used to identify the independent prognostic factors. We found that increased levels of LDH had poor effects on both overall survival and distant metastasis-free survival (P = 0.009 and 0.035, respectively), and increased pretreatment level of serum ALP had poor effects on both overall survival and local recurrence-free survival (P = 0.037 and 0.039, respectively). In multivariate analysis, increased LDH level was identified as an independent prognostic factor for overall survival. Therefore, we conclude that increased pretreatment serum LDH and ALP levels are poor prognostic factors for NPC.
Adolescent
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Adult
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Aged
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Alkaline Phosphatase
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blood
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Antineoplastic Combined Chemotherapy Protocols
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therapeutic use
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Child
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Cisplatin
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administration & dosage
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Female
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Fluorouracil
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administration & dosage
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Humans
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Kaplan-Meier Estimate
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L-Lactate Dehydrogenase
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blood
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Male
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Middle Aged
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Nasopharyngeal Neoplasms
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blood
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drug therapy
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pathology
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radiotherapy
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Neoplasm Metastasis
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Neoplasm Recurrence, Local
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Prognosis
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Proportional Hazards Models
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Radiotherapy, Computer-Assisted
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Radiotherapy, Conformal
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Radiotherapy, Intensity-Modulated
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Survival Rate
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Young Adult
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gamma-Glutamyltransferase
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blood
4.Effect of temperature on hospital admission among patients with chronic systolic heart failure.
Wen-fang XIA ; Qi-zhu TANG ; Sheng-bo YU ; Hong-ying CUI ; Mu QIN ; Tao LIU ; Bin KONG ; Qing-yan ZHAO ; He HUANG ; Cong-xin HUANG
Chinese Journal of Epidemiology 2013;34(1):67-70
OBJECTIVETo investigate the effect of temperature on hospital admission among patients with chronic systolic heart failure (CSHF).
METHODSData regarding in-hospital patients with CSHF were gathered from 12 hospitals in Hubei province, between 2000 and 2010. Patients with a history of congenital heart disease and the history of cancer from this series, were excluded. Chi-square (χ(2)) tests and t tests were used for descriptive analysis. Univariate and multivariate logistic regression methods were performed to determinate the risk of hospital admission of every month to compare with the previous one. We used 2-tailed 95% confidence interval (CI), and tests with P < 0.01 to consider the significant levels, statistically. We also used the SPSS 13.0 for Windows, release 15, 2006 (SPSS Inc, Chicago, Ill) for data analyses.
RESULTS(1) 48 964 patients were enrolled in the present study. The numbers of admission increased 18.71%, 13.84%, -21.90%, -34.62%, -21.97%, -3.81%, -2.04%, 10.13%, -17.13%, -0.85%, 21.54% and 42.70% from January to December when compared to the average number of admission. (2) The odds ratios (ORs) (95% CI, P values) of hospital admission in January, February and December were 1.09 (0.96 - 1.23, 0.54), 0.98 (0.84 - 1.10, 0.46) and 0.96 (0.84 - 1.08, 0.59), respectively in females which did not show any significant differences when compared to the number in August. However the ratios were 0.61 (0.54 - 0.69, < 0.01), 0.80 (0.68 - 0.92, < 0.01) and 0.73 (0.64 - 0.83, < 0.01), respectively, in males that showed significant differences when, compared to the figures in August. (3) The OR of admission increased more when temperature got lower for patients with coronary artery disease, hypertension heart disease or rheumatic heart disease, but not with dilated cardiomyopathy. (4) The OR of admission showed a different impact on patients with different occupation, along with the change of temperature. Low or high temperature did not seem to have different effects on the OR of admission in patients who were free-lanced or unemployed.
CONCLUSIONTemperature seemed to have significant effects on the risk of admission, which related to gender, etiology or occupation.
Adult ; Aged ; Aged, 80 and over ; Chronic Disease ; Climate ; Female ; Heart Failure ; Humans ; Inpatients ; statistics & numerical data ; Logistic Models ; Male ; Middle Aged ; Retrospective Studies ; Risk Factors ; Temperature
5.Evaluation of the effect of up-converting phosphor technology in detection of plague antigen-antibody by receiver operating characteristic curve method
Jin-xiao, XI ; Hong, ZHANG ; Peng-fei, GE ; Li-min, GUO ; Wen-ping, DA ; Bin, WU ; Da-qin, XU ; Shi-ming, WANG ; Wei-min, PAN ; Ke-jun, MIAO ; Ya-jun, GE ; Tao-xia, MU ; Guo-juan, CHEN
Chinese Journal of Endemiology 2012;31(6):640-642
Objective To evaluate the effect of up-converting phosphor technology(UPT) in detection of plague antigen-antibody by receiver operating characteristic curve (ROC) method,and to provide a scientific basis for field application of UPT rapid detection technology in plague prevention and control.Methods Two hundred and twenty four serum samples were collected from Marmots and ground squirrels in the plague foci,Yersinia pestis antibody was detected by UPT,ELISA,Colloidal-gold Strips and IHA,respectively; 108 organs and bone marrow samples were collected,and Yersinia pestis antigens were detected by UPT,ELISA,PCR and RIHA,respectively.IHA was used as the gold standard for antibody test results,RIHA,PCR + Colloidal-gold Strips,PCR + ELISA were used as the gold standard for antigen test results.The results were evaluated using ROC method.Results Antibodies detection:the AUCs of UPT,ELISA and Colloidal-gold Strips were greater than 0.5.The difference between UPT and other methods was not statistically significant (z =1.204,P > 0.05).Antigen detection:the AUCs of UPT,ELISA,Colloidal-gold Strips and PCR were greater than 0.5.There was no statistical difference between UPT and other methods(z =0.866,P > 0.05).Conclusions UPT as a new technology works well in the detection of plague antigen-antibody.The technology is simple,fast,accurate,and suitable for on-site monitoring of plague,emergency treatment of sudden plague,and suitable for promotion.
6.Study on genotypes and geographical distribution of 58 strains of Yersinia pestis in Gansu.
Peng-fei GE ; Hong ZHANG ; Zhao-biao GUO ; Ke-jun MIAO ; Da-qin XU ; Wen-ping DA ; Bin WU ; Li-min GUO ; Ya-jun GE ; Guo-juan CHEN ; Tao-xia MU ; Jin-xiao XI
Chinese Journal of Epidemiology 2010;31(7):840-840
Animals
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China
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epidemiology
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Genotype
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Geography
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Humans
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Marmota
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microbiology
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Plague
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epidemiology
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microbiology
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Yersinia pestis
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classification
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genetics
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isolation & purification
7.Analysis on genotype distributions and epidemiological characteristics of Yersinia pestis plague foci in Gansu province.
Xin-hua WANG ; Hong ZHANG ; Li-min GUO ; Ke-jun MIAO ; Wen-ping DA ; Bin WU ; Da-qin XU ; Ya-jun GE ; Tao-xia MU ; Guo-juan CHEN ; Xiao-yan ZHOU ; Shi-ming WANG ; Peng-fei GE ; Jin-xiao XI
Chinese Journal of Epidemiology 2013;34(5):433-437
OBJECTIVETo study the genotype distributions and epidemiological characteristics of Yersinia pestis in Gansu province.
METHODSPrimers were designed according to the confirmed 23 differential sections, to genotype the 202 Yersinia pestis DNA of Gansu province by PCR, and to analyze its distribution and epidemiological characteristics.
RESULTSYersinia pestis in Gansu province could be divided into eight genotypes: 1b, 5, 7, 8, 13, 26, new genotype 1 (GS1) and new genotype 2 (GS2). They were distributed in various regions. 1b, 8 and GS1 genotypes of Yersinia pestis had been identified since 1960s but the 7, 13 and 26 genotypes had not been isolated for more than 40 years while GS2 and 5 genotypes had been isolated since 1990s.
CONCLUSION1b, 8 and GS1 genotypes of Yersinia pestis continued to be violently prevalent since 1960s but 7, 13 and 26 genotypes had not been isolated for more than 40 years while GS2 and 5 genotypes had started to be popular since 1990s.
Animals ; China ; epidemiology ; DNA Primers ; Genetic Variation ; Genome, Bacterial ; Genotype ; Humans ; Plague ; epidemiology ; microbiology ; Yersinia pestis ; genetics ; isolation & purification
8.Effectiveness of azithromycin mass drug administration on trachoma: a systematic review.
Tao XIONG ; Yan YUE ; Wen-Xing LI ; Imti CHOONARA ; Shamim QAZI ; Hong-Ju CHEN ; Jun TANG ; Jing SHI ; Hua WANG ; Li-Nan ZENG ; Bin XIA ; Li-Na QIAO ; Yi QU ; De-Zhi MU
Chinese Medical Journal 2021;134(24):2944-2953
BACKGROUNDS:
Azithromycin mass drug administration (MDA) is a key part of the strategy for controlling trachoma. This systematic review aimed to comprehensively summarize the present studies of azithromycin MDA on trachoma; provide an overview of the impact of azithromycin MDA on trachoma in different districts; and explore the possible methods to enhance the effectiveness of azithromycin MDA in hyperendemic districts.
METHODS:
PubMed, Embase, the Cochrane Central Register of Controlled Trials, Web of Science, and ClinicalTrials.gov were searched up to February 2021 with no language restriction. Studies reporting the effect of azithromycin MDA on trachoma were included. Mathematical modeling studies, animal studies, case reports, and reviews were excluded. The trachomatous inflammation-follicular (TF) <5.0% was used to judge the effect of azithromycin MDA on eliminating trachoma as a public health problem. Two researchers independently conducted the selection process and risk of bias assessment.
RESULTS:
A total of 1543 studies were screened, of which 67 studies including 13 cluster-randomized controlled trials and 54 non-randomized studies were included. The effect of azithromycin MDA on trachoma was closely related to the baseline prevalence in districts. For the districts with baseline prevalence between 5.0% and 9.9%, a single round of MDA achieved a TF <5.0%. For the districts with baseline between 10.0% and 29.9%, annual MDA for 3 to 5 years reduced TF <5.0%. However, for the districts with high level of baseline prevalence (TF >30.0%), especially with baseline TF >50.0%, annual MDA was unable to achieve the TF <5.0% even after 5 to 7 years of treatment. Quarterly MDA is more effective in controlling trachoma in these hyperendemic districts.
CONCLUSIONS
Azithromycin MDA for controlling trachoma depends on the baseline prevalence. The recommendation by the World Health Organization that annual MDA for 3 to 5 years in the districts with TF baseline >10.0% is not appropriate for all eligible districts.
Anti-Bacterial Agents/therapeutic use*
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Azithromycin/therapeutic use*
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Humans
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Infant
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Mass Drug Administration
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Prevalence
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Trachoma/epidemiology*
9.Inverted U-Shaped Associations between Glycemic Indices and Serum Uric Acid Levels in the General Chinese Population: Findings from the China Cardiometabolic Disease and Cancer Cohort (4C) Study.
Yuan Yue ZHU ; Rui Zhi ZHENG ; Gui Xia WANG ; Li CHEN ; Li Xin SHI ; Qing SU ; Min XU ; Yu XU ; Yu Hong CHEN ; Xue Feng YU ; Li YAN ; Tian Ge WANG ; Zhi Yun ZHAO ; Gui Jun QIN ; Qin WAN ; Gang CHEN ; Zheng Nan GAO ; Fei Xia SHEN ; Zuo Jie LUO ; Ying Fen QIN ; Ya Nan HUO ; Qiang LI ; Zhen YE ; Yin Fei ZHANG ; Chao LIU ; You Min WANG ; Sheng Li WU ; Tao YANG ; Hua Cong DENG ; Jia Jun ZHAO ; Lu Lu CHEN ; Yi Ming MU ; Xu Lei TANG ; Ru Ying HU ; Wei Qing WANG ; Guang NING ; Mian LI ; Jie Li LU ; Yu Fang BI
Biomedical and Environmental Sciences 2021;34(1):9-18
Objective:
The relationship between serum uric acid (SUA) levels and glycemic indices, including plasma glucose (FPG), 2-hour postload glucose (2h-PG), and glycated hemoglobin (HbA1c), remains inconclusive. We aimed to explore the associations between glycemic indices and SUA levels in the general Chinese population.
Methods:
The current study was a cross-sectional analysis using the first follow-up survey data from The China Cardiometabolic Disease and Cancer Cohort Study. A total of 105,922 community-dwelling adults aged ≥ 40 years underwent the oral glucose tolerance test and uric acid assessment. The nonlinear relationships between glycemic indices and SUA levels were explored using generalized additive models.
Results:
A total of 30,941 men and 62,361 women were eligible for the current analysis. Generalized additive models verified the inverted U-shaped association between glycemic indices and SUA levels, but with different inflection points in men and women. The thresholds for FPG, 2h-PG, and HbA1c for men and women were 6.5/8.0 mmol/L, 11.0/14.0 mmol/L, and 6.1/6.5, respectively (SUA levels increased with increasing glycemic indices before the inflection points and then eventually decreased with further increases in the glycemic indices).
Conclusion
An inverted U-shaped association was observed between major glycemic indices and uric acid levels in both sexes, while the inflection points were reached earlier in men than in women.
Aged
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Asian Continental Ancestry Group
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Blood Glucose/analysis*
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China/epidemiology*
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Cohort Studies
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Diabetes Mellitus/blood*
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Female
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Glucose Tolerance Test
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Glycated Hemoglobin A/analysis*
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Glycemic Index
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Humans
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Male
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Middle Aged
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Uric Acid/blood*